Panama disease, or Fusarium wilt, the most serious disease in banana cultivation, is caused by Fusarium oxysporum f. sp. cubense (FOC) and has led to great economic losses worldwide. One effective way to combat this disease is by enhancing host plant resistance. The cerato-platanin protein (CPP) family is a group of small secreted cysteine-rich proteins in filamentous fungi. CPPs as elicitors can trigger the immune system resulting in defense responses in plants. In this study, we characterized a novel cerato-platanin-like protein in the secretome of Fusarium oxysporum f. sp. cubense race 4 (FOC4), named FocCP1. In tobacco, the purified recombinant FocCP1 protein caused accumulation of reactive oxygen species (ROS), formation of necrotic reaction, deposition of callose, expression of defense-related genes, and accumulation of salicylic acid (SA) and jasmonic acid (JA) in tobacco. These results indicated that FocCP1 triggered a hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco. Furthermore, FocCP1 enhanced resistance tobacco mosaic virus (TMV) disease and Pseudomonas syringae pv. tabaci 6605 (Pst. 6605) infection in tobacco and improved banana seedling resistance to FOC4. All results provide the possibility of further research on immune mechanisms of plant and pathogen interactions, and lay a foundation for a new biological strategy of banana wilt control in the future.

Melatonin (MEL) is a pleiotropic agent with crucial functions reported in a variety of stress responses and developmental processes. Although MEL involvement in plant defense against natural leaf senescence has been widely reported, the precise regulatory mechanisms by which it delays stress-induced senescence remain unclear. In this study, we found that foliar spraying of melatonin markedly ameliorated dehydration-induced leaf senescence in Nicotiana tabacum, accompanied by attenuated oxidative damage, expression of senescence-related genes, and reduced endogenous ABA production. Metabolite profiling indicated that melatonin-treated plants accumulated higher concentrations of sugars, sugar alcohol, and organic acids, but fewer concentrations of amino acids in the leaves, than untreated plants after exposure to dehydration. Gene expression analysis revealed that the delayed senescence of stressed plants achieved by melatonin treatment might be partially ascribed to the upregulated expression of genes involved in ROS scavenging, chlorophyll biosynthesis, photosynthesis, and carbon/nitrogen balances, and downregulated expression of senescence-associated genes. Furthermore, hormone responses showed an extensively modulated expression, complemented by carotenoid biosynthesis regulation to achieve growth acceleration in melatonin-treated plants upon exposure to dehydration stress. These findings may provide more comprehensive insights into the role of melatonin in alleviating leaf senescence and enhancing dehydration resistance.

The fabrication of durable materials with excellent oil-adsorption capacity and separation performance for the treatment of oily wastewater is meaningful based on the special property of smart responsiveness. Herein, a solvent-responsive melamine sponge (MS) was developed via silanization and the in situ growth of a zeolitic imidazolate framework-8 (ZIF-8). Detailed characterization of the resultant composite MS was conducted using scanning electron microscopy (SEM), energy-dispersive spectrometry (EDS), and X-ray diffraction (XRD). The multiscale hierarchical MS substrate exhibited highly hydrophobic properties in the pH range of 1–11, along with a satisfactory adsorption capacity in the range of 65.4–134.2 g/g for different oils. The modified surface transformed from superhydrophobic/superlipophilic to superhydrophilic/underwater superoleophobic upon ethanol wetting, reverting to its original superhydrophobic state upon drying. The separation flux of the MS substrate was above 1.5 × 10⁴ L/m²h for both oil and water removal, and the separation efficiency was greater than 98.7%. The absence of obvious changes in separation performance after 50 successive immiscible oil−water separations indicated the excellent durability and robustness of the anchored ZIF-8 nanoparticles on the surface of the modified MS substrate. More importantly, oil-in-water emulsion separation was successfully carried out via the ZIF-8 MS composite, showing high separation efficiency (over 99.1%). The developed smart sponge, which had high oil-adsorption capacity, excellent chemical stability, and fire resistance, has a wide range of potential practical applications in the convenient treatment of oily wastewater.

Fabrication of durable materials with excellent oil adsorption capacity and separation performance for the treatment of oily wastewater is meaningful based on smart responsiveness. Herein, a solvent-responsive melamine sponge (MS) was developed via silanization followed by the in-situ growth of zeolitic imidazolate framework-8 (ZIF-8). Detailed characterization of the resultant composite MS was conducted using scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The multiscale hierarchical MS substrate exhibited highly hydrophobic property in the pH range of 1-11, along with a satisfactory adsorption capacity in the range of 65.4-134.2 g/g for different oils. The modified surface transformed from superhydrophobicity/superlipophilicity to superhydrophilicity/underwater superoleophobicity upon ethanol wetting, reverting to its original superhydrophobic state upon drying. The separation flux of the MS substrate was above 1.5×104L/m2h for both oil and water removal, along with the separation efficiency more than 98.7%. No obvious changes of the separation performance after 50 successive immiscible oil/water separation indicated the excellent durability and robustness of the anchored ZIF-8 nanoparticles on the surface of modified MS substrate. More importantly, oil-in-water emulsion separation was successfully carried out via the ZIF-8 MS composite with a high separation efficiency over 99.1%. The developed smart sponge with high oil absorption capacity, excellent chemical stability and fire resistance exhibited a wide range of potential practical applications in the convenient treatment of oily wastewater.

Introduction: Polyglutamic acid (γ-PGA) is a promising biostimulant for enhancing crop growth and stress resistance, while its agricultural application is limited by poor leaf retention, low mobility within plants, and susceptibility to rain wash-off. Methods: This study developed PGA nanofertilizers via a facile one-step self-assembly strategy in crude γ-PGA aqueous solution without adding salt ions to overcome these limitations. SEM images show that the obtained nanoparticles appear uniform spherical morphology and good dispersibility in water with an average hydrodynamic diameter of 182 nm confirmed by DLS. XRD and DSC patterns indicate a strong reduction in crystallinity consistent with a largely amorphous or highly disordered state. Results: Fluorescence imaging of FITC-labeled PGA nanofertilizers (FITC@PGA) indicates systemic, vascular-localized signals consistent with bidirectional movement from absorption of both roots and leaves. Importantly, the PGA nanofertilizers exhibited superior rainfastness and leaf retention compared to crude γ-PGA. Physiological assessments showed that foliar application of PGA nanofertilizers significantly enhanced chlorophyll content, root development, and antioxidant enzyme activities compared to that of crude γ-PGA, which led to significant improvement for tomato growth and stress tolerance. Discussion: It is clear shown that the nano-engineering strategy will provide a promising approach for developing efficient and eco-friendly nanofertilizer.

Habitat and temporal variation can both influence microbial community dynamics, although their relative importance in reservoir buffer zones with complex hydrology regimes and dramatically altered environments remains controversial. To elucidate this, we investigated spatiotemporal variation in soil bacterial diversity and ecological processes from the flooding period to the dry period (April and June, respectively) using high‐throughput 16S amplicon sequencing in three habitats (abandoned cropland, grassland, and woodland) within the Chushandian Reservoir's buffer strip, China. The results showed that habitat was more important than temporal variation in shaping soil bacterial diversity and ecological processes in the reservoir buffer zone. Bacterial communities responded to temporal variation both in terms of species composition and function; temporal variation affected bacterial communities mostly by altering the abundance of shared species and by causing the resurgence or extinction of specific taxa within the same habitat. The main driver of these changes was the resilience capacity of habitats to the changing moisture environment. The magnitude and underlying mechanisms of the changes in bacterial community diversity and ecological processes differed markedly between the three habitats, owing mostly to the characteristics of their vegetation, thus the allocation ratios of different habitat vegetation types and landscape diversity should pay attention for the reservoir buffer zone management, improving the integrated ecological benefits of the reservoir ecosystem from a multiscale.