Inland waters transport large amounts of dissolved organic matter (DOM) from terrestrial environments to the oceans, but DOM also reacts en route, with substantial water column losses by mineralization and sedimentation. For DOM transformations along the aquatic continuum, lakes play an important role as they retain waters in the landscape allowing for more time to alter DOM. We know DOM losses are significant at the global scale, yet little is known about how the reactivity of DOM varies across landscapes and climates. DOM reactivity is inherently linked to its chemical composition. We used fluorescence spectroscopy to explore DOM quality from 560 lakes distributed across Sweden and encompassed a wide climatic gradient typical of the boreal ecozone. Six fluorescence components were identified using parallel factor analysis (PARAFAC). The intensity and relative abundance of these components were analyzed in relation to lake chemistry, catchment, and climate characteristics. Land cover, particularly the percentage of water in the catchment, was a primary factor explaining variability in PARAFAC components. Likewise, lake water retention time influenced DOM quality. These results suggest that processes occurring in upstream water bodies, in addition to the lake itself, have a dominant influence on DOM quality. PARAFAC components with longer emission wavelengths, or red‐shifted components, were most reactive. In contrast, protein‐like components were most persistent within lakes. Generalized characteristics of PARAFAC components based on emission wavelength could ease future interpretation of fluorescence spectra. An important secondary influence on DOM quality was mean annual temperature, which ranged between −6.2 and +7.5 °C. These results suggest that DOM reactivity depends more heavily on the duration of time taken to pass through the landscape, rather than temperature. Projected increases in runoff in the boreal region may force lake DOM toward a higher overall amount and proportion of humic‐like substances.

Total organic carbon (TOC) concentrations from seven boreal catchments in northern Sweden were monitored between June 1996 and May 1998 to examine spatial and temporal variations in streamwater TOC export and its relationship with catchment characteristics. The annual average export of TOC ranged between 36 and 76 kg ha^–1 yr^–1 and correlated positively with the areal extent of wetlands (r² = 0.72; p = 0.03). The daily output of TOC was 5–11 times higher during the spring than during any other season. In total, the four week long spring period contributed between 50% and 68% of the annual TOC export from the seven catchments. The relative importance of the spring snow melt period for the annual TOC export, however, correlated negatively with the percentage of wetlands (r² = 0.83; p<0.01). We suggest that the smaller relative importance of the spring runoff period for the annual TOC export from wetland dominated catchments is a result of the hydrological flow paths associated with the snow melt period. While a large fraction of the spring runoff from forested areas reaches the stream via subsurface flow paths across riparian soils rich in TOC, the flow paths through wetland dominated systems include a much larger component of low-TOC snow melt water via surface flow over ice and frozen peat.