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Vasile Coman
Institute of Life Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400372 Cluj-Napoca, Romania

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Journal article
Published: 20 July 2021 in Algal Research
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Recent advances in functional foods and nutraceuticals have emphasized the beneficial impact of bioactive molecules on human health and longevity. The omega-3 (ω-3) long-chain polyunsaturated fatty acids (LC-PUFAs), namely eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have been shown to exert numerous positive effects on cardiovascular events, inflammatory disease, some cancers, and neurological disorders. The primary sources of ω-3 LC-PUFAs for human consumption are cold-water fish and seafood; however, these are harvested currently beyond a sustainable capacity. Therefore, new sources of ω-3 LC-PUFAs such as oleaginous microalgae are regarded as promising alternatives to fish and seafood. In this context, the aim of the present review is to summarize the recent biotechnological progress made regarding the production, purification, and concentration of ω-3 LC-PUFAs from marine microalgae.

ACS Style

Daniel Gabriel Barta; Vasile Coman; Dan Cristian Vodnar. Microalgae as sources of omega-3 polyunsaturated fatty acids: Biotechnological aspects. Algal Research 2021, 58, 102410 .

AMA Style

Daniel Gabriel Barta, Vasile Coman, Dan Cristian Vodnar. Microalgae as sources of omega-3 polyunsaturated fatty acids: Biotechnological aspects. Algal Research. 2021; 58 ():102410.

Chicago/Turabian Style

Daniel Gabriel Barta; Vasile Coman; Dan Cristian Vodnar. 2021. "Microalgae as sources of omega-3 polyunsaturated fatty acids: Biotechnological aspects." Algal Research 58, no. : 102410.

Review
Published: 23 September 2020 in Experimental Gerontology
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Our gut microbiota is a complex and dynamic ecosystem with a paramount role in shaping our metabolic and immunological functions. Recent research suggests that aging may negatively affect the composition, diversity, and function of our microbiota mainly due to alterations in diet and immunologic reactivity (i.e. immunosenescence), and increased incidence of certain diseases and, therefore, increased exposure to certain medication (e.g. antibiotics, proton pump inhibitors). In turn, this aging-related gut dysbiosis may contribute to the initiation and/or progress of other metabolic diseases, and consequently, to a decrease in healthy longevity. On the positive side, promising therapeutic interventions, such as diet supplementation with prebiotics, probiotics and synbiotics, or fecal microbiota transplantation, aimed to counteract these aging-related deleterious consequences, could improve our health, and extend our healthy lifespan. In this context, the current review aims to assess the latest progress in identifying the key elements affecting the gut microbiota of the older adults and their mechanism of action, and the effectiveness of the therapeutic interventions aimed at restoring the diversity and healthy functions of the gut microbiota in older individuals.

ACS Style

Vasile Coman; Dan Cristian Vodnar. Gut microbiota and old age: Modulating factors and interventions for healthy longevity. Experimental Gerontology 2020, 141, 111095 -111095.

AMA Style

Vasile Coman, Dan Cristian Vodnar. Gut microbiota and old age: Modulating factors and interventions for healthy longevity. Experimental Gerontology. 2020; 141 ():111095-111095.

Chicago/Turabian Style

Vasile Coman; Dan Cristian Vodnar. 2020. "Gut microbiota and old age: Modulating factors and interventions for healthy longevity." Experimental Gerontology 141, no. : 111095-111095.

Journal article
Published: 22 May 2020 in Plants
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An efficient micropropagation protocol for Rheum rhabarbarum L. was developed in this study. The in vitro rhubarb plants obtained in the multiplication stage (proliferation rate: 5.0 ± 0.5) were rooted in vitro (96% rooting percentage) and acclimatized ex vitro in floating perlite, with 90% acclimatization percentage. To assess the genetic fidelity between the mother plant and in vitro propagated plants, sequence-related amplified polymorphism (SRAP) markers were used. All banding profiles from the micropropagated plants were monomorphic and similar to those of the mother plant indicating 100% similarity. Regarding the polyphenolic profile, gallic, protocatechuic, p-hydroxybenzoic, vanillic, chlorogenic, caffeic, syringic, p-coumaric and ferulic acid were present in different amounts (2.3–2690.3 μg g−1 dry plant), according to the extracted matrix. Aglicons and glycosides of different classes of flavonoids were also identified. The rhizome extracts (both from in vitro and field grown plants) contained resveratrol, a stilbene compound with high antioxidant properties, ranging between 229.4 to 371.7 μg g−1 plant. Our results suggest that in vitro propagation of Rheum rhabarbarum L. represents a reliable alternative to obtain a large number of true-to-type planting material with high bioactive compound content of this valuable nutritional and medicinal species.

ACS Style

Doina Clapa; Orsolya Borsai; Monica Hârța; Victoriţa Bonta; Katalin Szabo; Vasile Coman; Otilia Bobiș. Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L. Plants 2020, 9, 656 .

AMA Style

Doina Clapa, Orsolya Borsai, Monica Hârța, Victoriţa Bonta, Katalin Szabo, Vasile Coman, Otilia Bobiș. Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L. Plants. 2020; 9 (5):656.

Chicago/Turabian Style

Doina Clapa; Orsolya Borsai; Monica Hârța; Victoriţa Bonta; Katalin Szabo; Vasile Coman; Otilia Bobiș. 2020. "Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L." Plants 9, no. 5: 656.

Review article
Published: 16 April 2020 in Critical Reviews in Biotechnology
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Dietary habits that include an excess of added sugars have been strongly associated with an increased risk of obesity, heart disease, diabetes, and tooth decay. With this association in view, modern food systems aim to replace added sugars with low calorie sweeteners, such as polyols. Polyols are generally not carcinogenic and do not trigger a glycemic response. Furthermore, owing to the absence of the carbonyl group, they are more stable compared to monosaccharides and do not participate in Maillard reactions. As such, since polyols are stable at high temperatures, and they do not brown or caramelize when heated. Therefore, polyols are widely used in the diets of hypocaloric and diabetic patients, as well as other specific cases where controlled caloric intake is required. In recent years, erythritol and mannitol have gained increased importance, especially in the food and pharmaceutical industries. In these areas, research efforts have been made to improve the productivity and yield of the two polyols, relying on biotechnological manufacturing methods. The present review highlights the recent advances in the biotechnological production of erythritol and mannitol and summarizes the benefits of using the two polyols in the food and pharmaceutical industries.

ACS Style

Gheorghe Adrian Martău; Vasile Coman; Dan Cristian Vodnar. Recent advances in the biotechnological production of erythritol and mannitol. Critical Reviews in Biotechnology 2020, 40, 608 -622.

AMA Style

Gheorghe Adrian Martău, Vasile Coman, Dan Cristian Vodnar. Recent advances in the biotechnological production of erythritol and mannitol. Critical Reviews in Biotechnology. 2020; 40 (5):608-622.

Chicago/Turabian Style

Gheorghe Adrian Martău; Vasile Coman; Dan Cristian Vodnar. 2020. "Recent advances in the biotechnological production of erythritol and mannitol." Critical Reviews in Biotechnology 40, no. 5: 608-622.

Book chapter
Published: 01 January 2020 in Advances in Food and Nutrition Research
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Fruits and vegetables are essential for human nutrition, delivering a substantial proportion of vitamins, minerals, and fibers in our daily diet. Unfortunately, half the fruits and vegetables produced worldwide end up as wastes, generating environmental issues caused mainly by microbial degradation. Most wastes are generated by industrial processing, the so-called by-products. These by-products still contain many bioactive compounds post-processing, such as macronutrients (proteins and carbohydrates) and phytochemicals (polyphenols and carotenoids). Recently, the recovery of these bioactive compounds from industry by-products has received significant attention, mainly due to their possible health benefits for humans. This chapter focuses on the bioactive potential of fruit and vegetable by-products with possible applications in the food industry (functional foods) and in the health sector (nutraceuticals).

ACS Style

Vasile Coman; Bernadette-Emőke Teleky; Laura Mitrea; Gheorghe Adrian Martău; Katalin Szabo; Lavinia-Florina Călinoiu; Dan Cristian Vodnar. Bioactive potential of fruit and vegetable wastes. Advances in Food and Nutrition Research 2020, 91, 157 -225.

AMA Style

Vasile Coman, Bernadette-Emőke Teleky, Laura Mitrea, Gheorghe Adrian Martău, Katalin Szabo, Lavinia-Florina Călinoiu, Dan Cristian Vodnar. Bioactive potential of fruit and vegetable wastes. Advances in Food and Nutrition Research. 2020; 91 ():157-225.

Chicago/Turabian Style

Vasile Coman; Bernadette-Emőke Teleky; Laura Mitrea; Gheorghe Adrian Martău; Katalin Szabo; Lavinia-Florina Călinoiu; Dan Cristian Vodnar. 2020. "Bioactive potential of fruit and vegetable wastes." Advances in Food and Nutrition Research 91, no. : 157-225.

Journal article
Published: 15 November 2019 in Molecules
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Currently, research studies on nanoparticle cytotoxicity, uptake or internalization into the body’s cells are of great interest for the improvement of diagnostic and therapeutic applications. We report here the synthesis and characterization of very stable novel warfarin-capped gold nanoparticles with an average diameter of 54 ± 10 nm which were prepared using sodium warfarin as a reducing agent. The nanoparticles were tested in terms of cytotoxicity and cellular internalization in vitro on two cell lines: normal lung fibroblast HFL-1 and human retinal pigment epithelial D407 cells. Our results showed that the normal lung fibroblast HFL-1 cells were more sensitive to the nanoparticle treatment compared to the human retinal pigment epithelial D407 cells. Moreover, any signs of potential cytotoxicity occurred during the first 24 h of treatment, the cellular viability remaining largely unchanged for longer exposure times. Transmission electron microscopy and dark field hyperspectral imaging revealed that the nanoparticles were effectively delivered and released to the HFL-1 and D407 cells’ cytoplasm. Our results provide valuable information to further investigate sodium warfarin-capped gold nanoparticles for possible biological applications.

ACS Style

Loredana Florina Leopold; Dumitriţa Rugină; Ioana Oprea; Zorița Diaconeasa; Nicolae Leopold; Maria Suciu; Vasile Coman; Dan Cristian Vodnar; Adela Pintea; Cristina Coman. Warfarin-Capped Gold Nanoparticles: Synthesis, Cytotoxicity, and Cellular Uptake. Molecules 2019, 24, 4145 .

AMA Style

Loredana Florina Leopold, Dumitriţa Rugină, Ioana Oprea, Zorița Diaconeasa, Nicolae Leopold, Maria Suciu, Vasile Coman, Dan Cristian Vodnar, Adela Pintea, Cristina Coman. Warfarin-Capped Gold Nanoparticles: Synthesis, Cytotoxicity, and Cellular Uptake. Molecules. 2019; 24 (22):4145.

Chicago/Turabian Style

Loredana Florina Leopold; Dumitriţa Rugină; Ioana Oprea; Zorița Diaconeasa; Nicolae Leopold; Maria Suciu; Vasile Coman; Dan Cristian Vodnar; Adela Pintea; Cristina Coman. 2019. "Warfarin-Capped Gold Nanoparticles: Synthesis, Cytotoxicity, and Cellular Uptake." Molecules 24, no. 22: 4145.

Review
Published: 13 October 2019 in Journal of the Science of Food and Agriculture
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There is an urgent need to improve human diet globally. Compelling evidence gathered over the past several decades suggests that a suboptimal diet is associated with many chronic diseases and may be responsible for more deaths than any other risks worldwide. The main components in our diet that need higher intake are whole grains, fruit and vegetables, and nuts and seeds; all of these are important sources of dietary fiber and polyphenols. The health benefits of dietary fiber and polyphenols are also supported by several decades of valuable research. However, the conclusions drawn from interventional human trials are not straightforward and the action mechanisms in improving human health are not fully understood. Moreover, there is a great inter-individual variation caused by different individual capabilities of processing, absorbing and using these compounds effectively. Data on the bioavailability and bioefficacy of hydroxycinnamic acids (HCAs) are limited when compared to other classes of polyphenols (e.g. anthocyanins). This review aims to summarize the latest research advances related to HCA bioavailability and their biological effects revealed by epidemiological data, pre-clinical and clinical studies. Moreover, we aim to review the effects of HCAs on gut microbiota diversity and function and its respective influence on host health. © 2019 Society of Chemical Industry.

ACS Style

Vasile Coman; Dan C Vodnar. Hydroxycinnamic acids and human health: recent advances. Journal of the Science of Food and Agriculture 2019, 100, 483 -499.

AMA Style

Vasile Coman, Dan C Vodnar. Hydroxycinnamic acids and human health: recent advances. Journal of the Science of Food and Agriculture. 2019; 100 (2):483-499.

Chicago/Turabian Style

Vasile Coman; Dan C Vodnar. 2019. "Hydroxycinnamic acids and human health: recent advances." Journal of the Science of Food and Agriculture 100, no. 2: 483-499.

Review
Published: 03 September 2019 in Nanomaterials
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With a continuous increase in the production and use in everyday life applications of engineered nanomaterials, concerns have appeared in the past decades related to their possible environmental toxicity and impact on edible plants (and therefore, upon human health). Soybean is one of the most commercially-important crop plants, and a perfect model for nanomaterials accumulation studies, due to its high biomass production and ease of cultivation. In this review, we aim to summarize the most recent research data concerning the impact of engineered nanomaterials on the soya bean, covering both inorganic (metal and metal-oxide nanoparticles) and organic (carbon-based) nanomaterials. The interactions between soybean plants and engineered nanomaterials are discussed in terms of positive and negative impacts on growth and production, metabolism and influences on the root-associated microbiota. Current data clearly suggests that under specific conditions, nanomaterials can negatively influence the development and metabolism of soybean plants. Moreover, in some cases, a possible risk of trophic transfer and transgenerational impact of engineered nanomaterials are suggested. Therefore, comprehensive risk-assessment studies should be carried out prior to any mass productions of potentially hazardous materials.

ACS Style

Vasile Coman; Ioana Oprea; Loredana Florina Leopold; Dan Cristian Vodnar; Cristina Coman. Soybean Interaction with Engineered Nanomaterials: A Literature Review of Recent Data. Nanomaterials 2019, 9, 1248 .

AMA Style

Vasile Coman, Ioana Oprea, Loredana Florina Leopold, Dan Cristian Vodnar, Cristina Coman. Soybean Interaction with Engineered Nanomaterials: A Literature Review of Recent Data. Nanomaterials. 2019; 9 (9):1248.

Chicago/Turabian Style

Vasile Coman; Ioana Oprea; Loredana Florina Leopold; Dan Cristian Vodnar; Cristina Coman. 2019. "Soybean Interaction with Engineered Nanomaterials: A Literature Review of Recent Data." Nanomaterials 9, no. 9: 1248.

Journal article
Published: 01 December 2014 in Waste Management
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This study refers to two chemical leaching systems for the base and precious metals extraction from waste printed circuit boards (WPCBs); sulfuric acid with hydrogen peroxide have been used for the first group of metals, meantime thiourea with the ferric ion in sulfuric acid medium were employed for the second one. The cementation process with zinc, copper and iron metal powders was attempted for solutions purification. The effects of hydrogen peroxide volume in rapport with sulfuric acid concentration and temperature were evaluated for oxidative leaching process. 2M H2SO4 (98% w/v), 5% H2O2, 25 °C, 1/10 S/L ratio and 200 rpm were founded as optimal conditions for Cu extraction. Thiourea acid leaching process, performed on the solid filtrate obtained after three oxidative leaching steps, was carried out with 20 g/L of CS(NH2)2, 6g/L of Fe(3+), 0.5M H2SO4, The cross-leaching method was applied by reusing of thiourea liquid suspension and immersing 5 g/L of this reagent for each other experiment material of leaching. This procedure has lead to the doubling and, respectively, tripling, of gold and silver concentrations into solution. These results reveal a very efficient, promising and environmental friendly method for WPCBs processing.

ACS Style

Ionela Birloaga; Vasile Coman; Bernd Kopacek; Francesco Vegliò. An advanced study on the hydrometallurgical processing of waste computer printed circuit boards to extract their valuable content of metals. Waste Management 2014, 34, 2581 -2586.

AMA Style

Ionela Birloaga, Vasile Coman, Bernd Kopacek, Francesco Vegliò. An advanced study on the hydrometallurgical processing of waste computer printed circuit boards to extract their valuable content of metals. Waste Management. 2014; 34 (12):2581-2586.

Chicago/Turabian Style

Ionela Birloaga; Vasile Coman; Bernd Kopacek; Francesco Vegliò. 2014. "An advanced study on the hydrometallurgical processing of waste computer printed circuit boards to extract their valuable content of metals." Waste Management 34, no. 12: 2581-2586.

Evaluation study
Published: 01 November 2013 in Waste Management
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This study focuses on the electrodeposition of Ni and Ni-Fe alloys from synthetic solutions similar to those obtained by the dissolution of electron gun (an electrical component of cathode ray tubes) waste. The influence of various parameters (pH, electrolyte composition, Ni(2+)/Fe(2+) ratio, current density) on the electrodeposition process was investigated. Scanning electron microscopy (SEM) and X-ray fluorescence analysis (XRFA) were used to provide information about the obtained deposits' thickness, morphology, and elemental composition. By controlling the experimental parameters, the composition of the Ni-Fe alloys can be tailored towards specific applications. Complementarily, the differences in the nucleation mechanisms for Ni, Fe and Ni-Fe deposition from sulfate solutions have been evaluated and discussed using cyclic voltammetry and potential step chronoamperometry. The obtained results suggest a progressive nucleation mechanism for Ni, while for Fe and Ni-Fe, the obtained data points are best fitted to an instantaneous nucleation model.

ACS Style

B. Robotin; Adriana Ispas; Vasile Coman; Andreas Bund; P. Ilea. Nickel recovery from electronic waste II Electrodeposition of Ni and Ni–Fe alloys from diluted sulfate solutions. Waste Management 2013, 33, 2381 -2389.

AMA Style

B. Robotin, Adriana Ispas, Vasile Coman, Andreas Bund, P. Ilea. Nickel recovery from electronic waste II Electrodeposition of Ni and Ni–Fe alloys from diluted sulfate solutions. Waste Management. 2013; 33 (11):2381-2389.

Chicago/Turabian Style

B. Robotin; Adriana Ispas; Vasile Coman; Andreas Bund; P. Ilea. 2013. "Nickel recovery from electronic waste II Electrodeposition of Ni and Ni–Fe alloys from diluted sulfate solutions." Waste Management 33, no. 11: 2381-2389.

Review article
Published: 30 April 2013 in Resources, Conservation and Recycling
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Nickel is an important metal, heavily utilized in industry mainly due to its anticorrosion properties. As a consequence, nickel containing wastes such as spent batteries and catalysts, wastewater and bleed-off electrolytes are generated in various processes. These wastes could have a negative impact on the environment and human health if they contaminate soil, water and air. The present review addresses the environmental and economical aspects of nickel recovery/removal from various types of wastes. The main physico-chemical technologies for processing various effluents and wastewaters containing nickel are reviewed and discussed. Nickel recovery from spent batteries, catalysts, electronic waste and other sources is described. Hydrometallurgical approaches are emphasized. Recovery of nickel from wastes is important not only for economical aspects, but also for environmental protection.

ACS Style

Vasile Coman; B. Robotin; P. Ilea. Nickel recovery/removal from industrial wastes: A review. Resources, Conservation and Recycling 2013, 73, 229 -238.

AMA Style

Vasile Coman, B. Robotin, P. Ilea. Nickel recovery/removal from industrial wastes: A review. Resources, Conservation and Recycling. 2013; 73 ():229-238.

Chicago/Turabian Style

Vasile Coman; B. Robotin; P. Ilea. 2013. "Nickel recovery/removal from industrial wastes: A review." Resources, Conservation and Recycling 73, no. : 229-238.

Journal article
Published: 01 February 2013 in Analytical and Bioanalytical Chemistry
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Conventionally, microbial bioelectrochemical assays have been conducted using immobilized cells on an electrode that is placed in an electrochemical batch cell. In this paper, we describe a developed microfluidic platform with integrated microelectrode arrays for automated bioelectrochemical assays utilizing a new double mediator system to map redox metabolism and screen for genetic modifications in Saccharomyces cerevisiae cells. The function of this new double mediator system based on menadione and osmium redox polymer (PVI-Os) is demonstrated. “Wiring” of S. cerevisiae cells using PVI-Os shows a significant improvement of bioelectrochemical monitoring in a microfluidic environment and functions as an effective immobilization matrix for cells that are not strongly adherent. The function of the developed microfluidic platform is demonstrated using two strains of S. cerevisiae, ENY.WA and its deletion mutant EBY44, which lacks the enzyme phosphoglucose isomerase. The cellular responses to introduced glucose and fructose were recorded for the two S. cerevisiae strains, and the obtained results are compared with previously published work when using an electrochemical batch cell, indicating that microfluidic bioelectrochemical assays employing the menadione–PVI-Os double mediator system provides an effective means to conduct automated microbial assays. Figure Microfluidic platform for bioelectrochemical assays using osmium redox polymer “wired” living yeast cells

ACS Style

Arto Heiskanen; Vasile Coman; Natalie Kostesha; David Sabourin; Nick Haslett; Keith Baronian; Lo Gorton; Martin Dufva; Jenny Emnéus. Bioelectrochemical probing of intracellular redox processes in living yeast cells—application of redox polymer wiring in a microfluidic environment. Analytical and Bioanalytical Chemistry 2013, 405, 3847 -3858.

AMA Style

Arto Heiskanen, Vasile Coman, Natalie Kostesha, David Sabourin, Nick Haslett, Keith Baronian, Lo Gorton, Martin Dufva, Jenny Emnéus. Bioelectrochemical probing of intracellular redox processes in living yeast cells—application of redox polymer wiring in a microfluidic environment. Analytical and Bioanalytical Chemistry. 2013; 405 (11):3847-3858.

Chicago/Turabian Style

Arto Heiskanen; Vasile Coman; Natalie Kostesha; David Sabourin; Nick Haslett; Keith Baronian; Lo Gorton; Martin Dufva; Jenny Emnéus. 2013. "Bioelectrochemical probing of intracellular redox processes in living yeast cells—application of redox polymer wiring in a microfluidic environment." Analytical and Bioanalytical Chemistry 405, no. 11: 3847-3858.

Comparative study
Published: 01 December 2012 in Bioelectrochemistry
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The electrochemical characterization of a class II cellobiose dehydrogenase (CDH), from the ascomycete fungus Neurospora crassa, adsorbed on graphite (G), was performed in regard to direct (DET) and mediated electron transfer (MET). The effects of the applied potential, mediator (1,4 benzoquinone) concentration and flow carrier pH on the amperometric response of the G/CDH modified electrodes were investigated under flow conditions. From the calibration curves, recorded at two pH values (5.2 and 7.0) for nine different sugars, the kinetic and the analytical parameters were evaluated under DET and MET operation modes. These results together with those obtained from long term operational stability measurements showed that: (i) for all nine investigated sugars the sensitivity was higher for MET than for DET and for pH 5.2 compared to pH 7.0; (ii) irrespective of DET or MET operation mode, the sensitivity of the new enzyme towards the investigated sugars decreased in the following sequence: cellobiose>lactose>(cellotriose≈cellopentaose) >(maltotriose≈maltotetraose≈maltopentaose)>(maltose≈glucose); (iii) for all tested substrates, the apparent CDH affinity was roughly higher in DET than in MET operation mode.

ACS Style

Gabor Kovacs; Roberto Ortiz; Vasile Coman; Wolfgang Harreither; Ion V. Popescu; Roland Ludwig; Lo Gorton. Graphite electrodes modified with Neurospora crassa cellobiose dehydrogenase: Comparative electrochemical characterization under direct and mediated electron transfer. Bioelectrochemistry 2012, 88, 84 -91.

AMA Style

Gabor Kovacs, Roberto Ortiz, Vasile Coman, Wolfgang Harreither, Ion V. Popescu, Roland Ludwig, Lo Gorton. Graphite electrodes modified with Neurospora crassa cellobiose dehydrogenase: Comparative electrochemical characterization under direct and mediated electron transfer. Bioelectrochemistry. 2012; 88 ():84-91.

Chicago/Turabian Style

Gabor Kovacs; Roberto Ortiz; Vasile Coman; Wolfgang Harreither; Ion V. Popescu; Roland Ludwig; Lo Gorton. 2012. "Graphite electrodes modified with Neurospora crassa cellobiose dehydrogenase: Comparative electrochemical characterization under direct and mediated electron transfer." Bioelectrochemistry 88, no. : 84-91.

Evaluation study
Published: 26 September 2012 in Journal of Laboratory Automation
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A microfluidic component library for building systems driving parallel or serial microfluidic-based assays is presented. The components are a miniaturized eight-channel peristaltic pump, an eight-channel valve, sample-to-waste liquid management, and interconnections. The library of components was tested by constructing various systems supporting perfusion cell culture, automated DNA hybridizations, and in situ hybridizations. The results showed that the MainSTREAM components provided (1) a rapid, robust, and simple method to establish numerous fluidic inputs and outputs to various types of reaction chips; (2) highly parallel pumping and routing/valving capability; (3) methods to interface pumps and chip-to-liquid management systems; (4) means to construct a portable system; (5) reconfigurability/flexibility in system design; (6) means to interface to microscopes; and (7) compatibility with tested biological methods. It was found that LEGO Mindstorms motors, controllers, and software were robust, inexpensive, and an accessible choice as compared with corresponding custom-made actuators. MainSTREAM systems could operate continuously for weeks without leaks, contamination, or system failures. In conclusion, the MainSTREAM components described here meet many of the demands on components for constructing and using microfluidics systems.

ACS Style

David Sabourin; Peder Skafte-Pedersen; Martin Jensen Søe; Mette Hemmingsen; Massimo Alberti; Vasile Coman; Jesper Petersen; Jenny Emnéus; Jörg P. Kutter; Detlef Snakenborg; Flemming Jørgensen; Christian Clausen; Kim Holmstrøm; Martin Dufva. The MainSTREAM Component Platform. Journal of Laboratory Automation 2012, 18, 212 -228.

AMA Style

David Sabourin, Peder Skafte-Pedersen, Martin Jensen Søe, Mette Hemmingsen, Massimo Alberti, Vasile Coman, Jesper Petersen, Jenny Emnéus, Jörg P. Kutter, Detlef Snakenborg, Flemming Jørgensen, Christian Clausen, Kim Holmstrøm, Martin Dufva. The MainSTREAM Component Platform. Journal of Laboratory Automation. 2012; 18 (3):212-228.

Chicago/Turabian Style

David Sabourin; Peder Skafte-Pedersen; Martin Jensen Søe; Mette Hemmingsen; Massimo Alberti; Vasile Coman; Jesper Petersen; Jenny Emnéus; Jörg P. Kutter; Detlef Snakenborg; Flemming Jørgensen; Christian Clausen; Kim Holmstrøm; Martin Dufva. 2012. "The MainSTREAM Component Platform." Journal of Laboratory Automation 18, no. 3: 212-228.

Journal article
Published: 22 February 2010 in Electroanalysis
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Due to the recent interest in enzymatic biofuel cells (BECs), sugar oxidizing enzymes other than the commonly used glucose oxidase are gaining more importance as possible bioelements of implantable microscale-devices, which can, for example, be used in biosensors and pacemakers. In this study we used rational and semi-rational protein design to improve the catalytic activity of the enzyme pyranose 2-oxidase (P2Ox) with its alternative soluble electron acceptors 1,4-benzoquinone and ferricenium ion, which can serve as electron mediators, to possibly boost the power output of enzymatic BECs. Using a screening assay based on 96-well plates, we identified the variant H450G, which showed lower K-M and higher k(cat) values for both 1,4-benzoquinone and ferricenium ion compared to the wild-type enzyme, when either D-glucose or D-galactose were used as saturating electron donors. Besides this variant, we analyzed the variants V546C and T169G/V546C for their possible application in enzymatic BECs. The results obtained in homogeneous solution were compared with those obtained when P2Ox was immobilized on the surface of graphite electrodes and either "wired" to an osmium redox polymer or using soluble 1,4-benzoquinone as mediator. According to the spectrophotometrically determined kinetic constants, the possible energy output, measured in flow injection analysis experiments with these variants, increased up to 4-fold compared to systems employing the wild-type enzyme. Our results show that by increasing the catalytic activity of the redox enzyme P2Ox with its alternative electron acceptors 1,4-benzoquinone and ferricenium ion, it is possible to achieve a higher energy output of an enzymatic BFC when using the same concentration of sugar substrate

ACS Style

Oliver Spadiut; Dagmar Brugger; Vasile Coman; Dietmar Haltrich; Lo Gorton. Engineered Pyranose 2-Oxidase: Efficiently Turning Sugars into Electrical Energy. Electroanalysis 2010, 22, 813 -820.

AMA Style

Oliver Spadiut, Dagmar Brugger, Vasile Coman, Dietmar Haltrich, Lo Gorton. Engineered Pyranose 2-Oxidase: Efficiently Turning Sugars into Electrical Energy. Electroanalysis. 2010; 22 (7-8):813-820.

Chicago/Turabian Style

Oliver Spadiut; Dagmar Brugger; Vasile Coman; Dietmar Haltrich; Lo Gorton. 2010. "Engineered Pyranose 2-Oxidase: Efficiently Turning Sugars into Electrical Energy." Electroanalysis 22, no. 7-8: 813-820.

Journal article
Published: 15 October 2009 in Journal of the American Chemical Society
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The present study explores genetic engineering of the respiratory chain and the application of two different flexible osmium redox polymers to achieve efficient electric communication between the gram-positive organism Bacillus subtilis and an electrode. Poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-bipyridyl)(2)Cl(2)](+/2+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for efficient electrical "wiring" of viable gram-positive bacterial cells to electrodes. Using a B. subtilis strain that overproduces succinate/quinone oxidoreductase (respiratory complex II), we were able to improve the current response several fold using succinate as substrate, in both batch and flow analysis modes, and using gold and graphite electrodes. The efficiency of the osmium redox polymer, working as electron transfer mediator between the cells and the electrode, was compared with that of a soluble mediator (hexacyanoferrate). The results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes

ACS Style

Vasile Coman; Tobias Gustavsson; Arnonas Finkelsteinas; Claes von Wachenfeldt; Cecilia Hägerhäll; Lo Gorton. Electrical Wiring of Live, Metabolically Enhanced Bacillus subtilis Cells with Flexible Osmium-Redox Polymers. Journal of the American Chemical Society 2009, 131, 16171 -16176.

AMA Style

Vasile Coman, Tobias Gustavsson, Arnonas Finkelsteinas, Claes von Wachenfeldt, Cecilia Hägerhäll, Lo Gorton. Electrical Wiring of Live, Metabolically Enhanced Bacillus subtilis Cells with Flexible Osmium-Redox Polymers. Journal of the American Chemical Society. 2009; 131 (44):16171-16176.

Chicago/Turabian Style

Vasile Coman; Tobias Gustavsson; Arnonas Finkelsteinas; Claes von Wachenfeldt; Cecilia Hägerhäll; Lo Gorton. 2009. "Electrical Wiring of Live, Metabolically Enhanced Bacillus subtilis Cells with Flexible Osmium-Redox Polymers." Journal of the American Chemical Society 131, no. 44: 16171-16176.

Original research paper
Published: 07 October 2009 in Fuel Cells
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We report on the fabrication and characterisation of the very first direct electron transfer-based glucose/oxygen biofuel cell (BFC) operating in neutral glucose-containing buffer and human serum. Corynascus thermophilus cellobiose dehydrogenase and Myrothecium verrucaria bilirubin oxidase were used as anodic and cathodic bioelements, respectively. The following characteristics of the mediator-, separator- and membrane-less, a priori, non-toxic and simple miniature BFC, was obtained: an open-circuit voltage of 0.62 and 0.58 V, a maximum power density of ca. 3 and 4 mu W cm(-2) at 0.37 and 0.19 V of cell voltage, in phosphate buffer and human serum, respectively

ACS Style

V. Coman; Roland Ludwig; W. Harreither; D. Haltrich; L. Gorton; T. Ruzgas; And S. Shleev. A Direct Electron Transfer-Based Glucose/Oxygen Biofuel Cell Operating in Human Serum. Fuel Cells 2009, 1 .

AMA Style

V. Coman, Roland Ludwig, W. Harreither, D. Haltrich, L. Gorton, T. Ruzgas, And S. Shleev. A Direct Electron Transfer-Based Glucose/Oxygen Biofuel Cell Operating in Human Serum. Fuel Cells. 2009; ():1.

Chicago/Turabian Style

V. Coman; Roland Ludwig; W. Harreither; D. Haltrich; L. Gorton; T. Ruzgas; And S. Shleev. 2009. "A Direct Electron Transfer-Based Glucose/Oxygen Biofuel Cell Operating in Human Serum." Fuel Cells , no. : 1.

Journal article
Published: 01 September 2009 in Electrochimica Acta
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In the present study three different strains of Escherichia coli (JM109 – a native “wild type” strain, JM109/pBSD 1300 – a strain overproducing the membrane anchor domain of Bacillus subtilis succinate-quinone reductase, SQR, a protein that contains two transmembraneously arranged heme groups and JM109/pLUV 1900 – a strain overproducing cytochrome c550 from B. subtilis, a protein where the cytochrome domain is anchored to the membrane with a transmembrane helix) were immobilised on the surface of a spectrographic graphite electrode and tested for electrical communication using mediators. Such compounds as ferricyanide, 2,6-dichlorophenolindophenol (DCPIP) and ubiquinone (Q0) were used as soluble mediators and two flexible osmium redox polymers; poly(1-vinylimidazole)12-[Os-(4,4′-dimethyl-2,2′-di’pyridyl)2Cl2]2+/+ (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N′-methylated-2,2′-biimidazole)3]2+/3+ (osmium redox polymer II) were co-immobilised with the bacterial cells onto the electrode surface. The effects of applied potential, buffer pH and different substrates were compared for the different combinations bacterial strains – mediators. Through the introduction of the cytochromes in the bacterial membrane it was established that it had great effect on the ability of the bacterial cells to effectively communicate with artificial mediators. The introduction of the transmembraneously arranged heme groups of B. subtilis made it possible for this strain to communicate with the Os-polymers, whereas the introduction of the cytochrome c550 had an effect especially increasing ability of Q0 to act as an efficient e− acceptor.

ACS Style

Sergey Alferov; Vasile Coman; Tobias Gustavsson; Anatoly Reshetilov; Claes von Wachenfeldt; Cecilia Hägerhäll; Lo Gorton. Electrical communication of cytochrome enriched Escherichia coli JM109 cells with graphite electrodes. Electrochimica Acta 2009, 54, 4979 -4984.

AMA Style

Sergey Alferov, Vasile Coman, Tobias Gustavsson, Anatoly Reshetilov, Claes von Wachenfeldt, Cecilia Hägerhäll, Lo Gorton. Electrical communication of cytochrome enriched Escherichia coli JM109 cells with graphite electrodes. Electrochimica Acta. 2009; 54 (22):4979-4984.

Chicago/Turabian Style

Sergey Alferov; Vasile Coman; Tobias Gustavsson; Anatoly Reshetilov; Claes von Wachenfeldt; Cecilia Hägerhäll; Lo Gorton. 2009. "Electrical communication of cytochrome enriched Escherichia coli JM109 cells with graphite electrodes." Electrochimica Acta 54, no. 22: 4979-4984.

Journals
Published: 01 January 2008 in Physical Chemistry Chemical Physics
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We report the fabrication and characterisation of a non-compartmentalised, mediator and cofactor free glucose–oxygen biofuel cell based on adsorbed enzymes exhibiting direct bioelectrocatalysis, viz. cellobiose dehydrogenase from Dichomera saubinetii and laccase from Trametes hirsuta as the anodic and cathodic bioelements, respectively, with the following characteristics: an open-circuit voltage of 0.73 V; a maximum power density of 5 μW cm−2 at 0.5 V of the cell voltage and an estimated half-life of >38 h in air-saturated 0.1 M citrate–phosphate buffer, pH 4.5 containing 5 mM glucose.

ACS Style

Vasile Coman; Cristina Vaz-Domínguez; Roland Ludwig; Wolfgang Harreither; Dietmar Haltrich; Antonio L. De Lacey; Tautgirdas Ruzgas; Lo Gorton; Sergey Shleev. A membrane-, mediator-, cofactor-less glucose/oxygen biofuel cell. Physical Chemistry Chemical Physics 2008, 10, 6093 -6096.

AMA Style

Vasile Coman, Cristina Vaz-Domínguez, Roland Ludwig, Wolfgang Harreither, Dietmar Haltrich, Antonio L. De Lacey, Tautgirdas Ruzgas, Lo Gorton, Sergey Shleev. A membrane-, mediator-, cofactor-less glucose/oxygen biofuel cell. Physical Chemistry Chemical Physics. 2008; 10 (40):6093-6096.

Chicago/Turabian Style

Vasile Coman; Cristina Vaz-Domínguez; Roland Ludwig; Wolfgang Harreither; Dietmar Haltrich; Antonio L. De Lacey; Tautgirdas Ruzgas; Lo Gorton; Sergey Shleev. 2008. "A membrane-, mediator-, cofactor-less glucose/oxygen biofuel cell." Physical Chemistry Chemical Physics 10, no. 40: 6093-6096.

Full paper
Published: 30 January 2007 in Electroanalysis
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The catalytic properties of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on a graphite electrode were investigated for a large variety of carbohydrate substrates. The effects of applied potential, pH and buffer composition were tested and optimized, and the most suitable conditions were used to evaluate the detection limit, linear range, and sensitivity of the sensor for different carbohydrates in the flow injection mode. Subsequently, the long term stability of the modified electrodes was determined. Additionally, the direct and mediated electron transfer between the active site of the enzyme and the electrode has been investigated by amperometric flow injection measurements in the absence and presence of the mediator 1,4‐benzoquinone in the presence of cellobiose or lactose.

ACS Style

Wolfgang Harreither; Vasile Coman; Roland Ludwig; Dietmar Haltrich; Lo Gorton. Investigation of Graphite Electrodes Modified with Cellobiose Dehydrogenase from the AscomyceteMyriococcum thermophilum. Electroanalysis 2007, 19, 172 -180.

AMA Style

Wolfgang Harreither, Vasile Coman, Roland Ludwig, Dietmar Haltrich, Lo Gorton. Investigation of Graphite Electrodes Modified with Cellobiose Dehydrogenase from the AscomyceteMyriococcum thermophilum. Electroanalysis. 2007; 19 (2-3):172-180.

Chicago/Turabian Style

Wolfgang Harreither; Vasile Coman; Roland Ludwig; Dietmar Haltrich; Lo Gorton. 2007. "Investigation of Graphite Electrodes Modified with Cellobiose Dehydrogenase from the AscomyceteMyriococcum thermophilum." Electroanalysis 19, no. 2-3: 172-180.