Fertilization and cultivation managements exert significant effects on crop growth and soil-associated nutrients in croplands. However, there is a lack of knowledge regarding how these practices affect soil phosphorus-cycling enzymes and functional genes involved in regulating global P-cycling, especially under intense agricultural management practices in sloping croplands. A long-term field (15-year) trial was conducted in a 15° sloping field based on five treatments: no fertilizer amendments + downslope cultivation (CK); mixed treatment of mineral fertilizer and organic manure + downslope cultivation (T1); mineral fertilizer alone + downslope cultivation (T2); 1.5-fold mineral fertilizer + downslope cultivation (T3); and mineral fertilizer + contour cultivation (T4). Bulk and rhizosphere soil samples were collected after the maize crop was harvested to determine the P fraction, P-cycling enzymes, and phosphatase-encoding genes. Results indicated that fertilization management significantly increased the inorganic (Pi) and organic soil (Po) P fractions compared to CK, except for NaOH-extractable Po in T1 and T3 in bulk and rhizosphere soils, respectively. For the cultivation treatments, the content of Pi pools in the downslope cultivation of T1 and T3 was significantly larger than that in the contour cultivation of T4 in bulk and rhizosphere soils. However, the content of NaOH-extractable Po in T1 and T3 was lower compared to T4 in bulk soil and vice versa for the NaHCO₃-P and HCl-Po fractions in the rhizosphere. We also found that fertilization and cultivation managements significantly increased the activity of acid phosphatase (ACP), alkaline phosphatase (ALP), phytase, phosphodiesterases (PDE), and phoC and phoD gene abundance in bulk and rhizosphere soils, with a larger effect on the activity of ALP and the phosphatase encoding phoD gene, especially in T1 and T3 in the rhizosphere. Soil organic carbon (SOC) and microbial biomass C and P (MBC and MBP) were the main predictors for regulating P-cycling enzymes and phoC- and phoD gene abundance. A strong association of P-cycling enzymes, especially ALP and phytase, and the abundance of phoD genes with the P fraction indicated that the soil P cycle was mainly mediated by microbial-related processes. Together, our results demonstrated that an adequate amount of mineral fertilizer alone or combined with organic fertilizer plus downslope cultivation is more effective in promoting soil P availability by enhancing the activity of ALP, phytase, and phoD genes. This provides valuable information for sustaining soil microbial-regulated P management practices in similar agricultural lands worldwide.

This study aims to investigate the roles of slope aspect, land use and soil depth in altering the soil organic carbon (C), total nitrogen (N), and total phosphorus (P) traits in the karst trough valley area experiencing extensive ecological restoration. A total of 54 soil samples were collected at 0–10, 10–20, and 20–30 cm soil depths from secondary forest, plantation forest, and grassland on the relatively more shaded east-facing slope and the contrasting west-facing slope, respectively. The independent and interactive effects of slope aspect, land use, and soil depth on soil C, N, and P concentrations and stoichiometry were determined. The results show that soil C and N concentrations were markedly higher on the east-facing slope than on the west-facing slope, and soil P concentrations showed an opposite trend, leading to significant differences in soil C:P and N:P but not in C:N ratios between the two aspects. Soil C and N concentrations were not affected by land use, and soil P concentration was significantly higher in plantation forest than in secondary forest and grassland. Soil C and N concentrations significantly decreased with increasing soil depth, but soil P concentration presented no significant changes with soil depth. Both the land use and soil depth did not differ in terms of their elemental stoichiometry. There were no significant interactive effects of slope aspect, land use and soil depth on soil C, N, and P traits. Our results indicate that soil C, N, and P changes are more sensitive to slope aspect rather than land use and soil depth in the karst trough valley area under ecological restoration.