This page has only limited features, please log in for full access.

Dr. Konstantin V. Kiselev
Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia

Basic Info

Basic Info is private.

Research Keywords & Expertise

0 Calcium
0 Gene Expression
0 phenolic compounds
0 resveratrol
0 Heat Stress

Fingerprints

Vitis amurensis
resveratrol
Gene Expression
stilbenes
Calcium
Calcium-dependent protein kinase
Arabidopsis thaliana
Piceid
phenolic compounds
Calmodulin-like proteins
Gene overexpression
Plant transgene

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 24 June 2021 in Plants
Reads 0
Downloads 0

Excessive ultraviolet B (UV-B) irradiation is one of the most serious threats leading to severe crop production losses. It is known that secondary metabolite biosynthesis plays an important role in plant defense and forms a protective shield against excessive UV-B irradiation. The contents of stilbenes and other plant phenolics are known to sharply increase after UV-B irradiation, but there is little direct evidence for the involvement of stilbenes and other plant phenolics in plant UV-B protection. This study showed that foliar application of trans-resveratrol (1 and 5 mM) and trans-piceid (5 mM) considerably increased tolerance to a shock of UV-B (10 min at 1800 µW cm−2 of irradiation intensity) of four-week-old Arabidopsis thaliana plants that are naturally incapable of stilbene production. Application of trans-resveratrol and trans-piceid increased the leaf survival rates by 1–2%. This stilbene-induced improvement in UV-B tolerance was higher than after foliar application of the stilbene precursors, p-coumaric and trans-cinnamic acids (only 1–3%), but less than that after treatment with octocrylene (19–24%), a widely used UV-B absorber. Plant treatment with trans-resveratrol increased expression of antioxidant and stress-inducible genes in A.thaliana plants and decreased expression of DNA repair genes. This study directly demonstrates an important positive role of stilbenes in plant tolerance to excessive UV-B irradiation, and offers a new approach for plant UV-B protection.

ACS Style

Zlata Ogneva; Vlada Volkonskaia; Alexandra Dubrovina; Andrey Suprun; Olga Aleynova; Konstantin Kiselev. Exogenous Stilbenes Improved Tolerance of Arabidopsis thaliana to a Shock of Ultraviolet B Radiation. Plants 2021, 10, 1282 .

AMA Style

Zlata Ogneva, Vlada Volkonskaia, Alexandra Dubrovina, Andrey Suprun, Olga Aleynova, Konstantin Kiselev. Exogenous Stilbenes Improved Tolerance of Arabidopsis thaliana to a Shock of Ultraviolet B Radiation. Plants. 2021; 10 (7):1282.

Chicago/Turabian Style

Zlata Ogneva; Vlada Volkonskaia; Alexandra Dubrovina; Andrey Suprun; Olga Aleynova; Konstantin Kiselev. 2021. "Exogenous Stilbenes Improved Tolerance of Arabidopsis thaliana to a Shock of Ultraviolet B Radiation." Plants 10, no. 7: 1282.

Journal article
Published: 23 June 2021 in Plants
Reads 0
Downloads 0

Plant endophytes are known to alter the profile of secondary metabolites in plant hosts. In this study, we identified the main bacterial and fungal representatives of the wild grape Vitis amurensis Rupr. microbiome and investigated a cocultivation effect of the 14 endophytes and the V. amurensis cell suspension on biomass accumulation and stilbene biosynthesis. The cocultivation of the V. amurensis cell culture with the bacteria Agrobacterium sp., Bacillus sp., and Curtobacterium sp. for 2 weeks did not significantly affect the accumulation of cell culture fresh biomass. However, it was significantly inhibited by the bacteria Erwinia sp., Pantoea sp., Pseudomonas sp., and Xanthomonas sp. and fungi Alternaria sp., Biscogniauxia sp., Cladosporium sp., Didymella sp. 2, and Fusarium sp. Cocultivation of the grapevine cell suspension with the fungi Didymella sp. 1 and Trichoderma sp. resulted in cell death. The addition of endophytic bacteria increased the total stilbene content by 2.2–5.3 times, while the addition of endophytic fungi was more effective in inducing stilbene accumulation by 2.6–16.3 times. The highest content of stilbenes in the grapevine cells cocultured with endophytic fungi was 13.63 and 13.76 mg/g of the cell dry weight (DW) after cultivation with Biscogniauxia sp. and Didymella sp. 2, respectively. The highest content of stilbenes in the grapevine cells cocultured with endophytic bacteria was 4.49 mg/g DW after cultivation with Xanthomonas sp. The increase in stilbene production was due to a significant activation of phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) gene expression. We also analyzed the sensitivity of the selected endophytes to eight antibiotics, fluconazole, and trans-resveratrol. The endophytic bacteria were sensitive to gentamicin and kanamycin, while all selected fungal strains were resistant to fluconazole with the exception of Cladosporium sp. All endophytes were tolerant of trans-resveratrol. This study showed that grape endophytes stimulate the production of stilbenes in grape cell suspension, which could further contribute to the generation of a new stimulator of stilbene biosynthesis in grapevine or grape cell cultures.

ACS Style

Olga Aleynova; Andrey Suprun; Nikolay Nityagovsky; Alexandra Dubrovina; Konstantin Kiselev. The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr. Plants 2021, 10, 1276 .

AMA Style

Olga Aleynova, Andrey Suprun, Nikolay Nityagovsky, Alexandra Dubrovina, Konstantin Kiselev. The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr. Plants. 2021; 10 (7):1276.

Chicago/Turabian Style

Olga Aleynova; Andrey Suprun; Nikolay Nityagovsky; Alexandra Dubrovina; Konstantin Kiselev. 2021. "The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr." Plants 10, no. 7: 1276.

Journal article
Published: 23 June 2021 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Exogenous application of double-stranded RNAs (dsRNAs) and small-interfering RNAs (siRNAs) to plant surfaces has emerged as a promising method for regulation of essential genes in plant pathogens and for plant disease protection. Yet, regulation of plant endogenous genes via external RNA treatments has not been sufficiently investigated. In this study, we targeted the genes of chalcone synthase (CHS), the key enzyme in the flavonoid/anthocyanin biosynthesis pathway, and two transcriptional factors, MYBL2 and ANAC032, negatively regulating anthocyanin biosynthesis in Arabidopsis. Direct foliar application of AtCHS-specific dsRNAs and siRNAs resulted in an efficient downregulation of the AtCHS gene and suppressed anthocyanin accumulation in A. thaliana under anthocyanin biosynthesis-modulating conditions. Targeting the AtMYBL2 and AtANAC032 genes by foliar dsRNA treatments markedly reduced their mRNA levels and led to a pronounced upregulation of the AtCHS gene. The content of anthocyanins was increased after treatment with AtMYBL2-dsRNA. Laser scanning microscopy showed a passage of Cy3-labeled AtCHS-dsRNA into the A. thaliana leaf vessels, leaf parenchyma cells, and stomata, indicating the dsRNA uptake and spreading into leaf tissues and plant individual cells. Together, these data show that exogenous dsRNAs were capable of downregulating Arabidopsis genes and induced relevant biochemical changes, which may have applications in plant biotechnology and gene functional studies.

ACS Style

Konstantin Kiselev; Andrey Suprun; Olga Aleynova; Zlata Ogneva; Alexander Kalachev; Alexandra Dubrovina. External dsRNA Downregulates Anthocyanin Biosynthesis-Related Genes and Affects Anthocyanin Accumulation in Arabidopsis thaliana. International Journal of Molecular Sciences 2021, 22, 6749 .

AMA Style

Konstantin Kiselev, Andrey Suprun, Olga Aleynova, Zlata Ogneva, Alexander Kalachev, Alexandra Dubrovina. External dsRNA Downregulates Anthocyanin Biosynthesis-Related Genes and Affects Anthocyanin Accumulation in Arabidopsis thaliana. International Journal of Molecular Sciences. 2021; 22 (13):6749.

Chicago/Turabian Style

Konstantin Kiselev; Andrey Suprun; Olga Aleynova; Zlata Ogneva; Alexander Kalachev; Alexandra Dubrovina. 2021. "External dsRNA Downregulates Anthocyanin Biosynthesis-Related Genes and Affects Anthocyanin Accumulation in Arabidopsis thaliana." International Journal of Molecular Sciences 22, no. 13: 6749.

Journal article
Published: 09 April 2021 in Metabolites
Reads 0
Downloads 0

Grapes and wines represent the most important source of edible stilbenes and other phenolic metabolites, which demonstrate a wide range of valuable biological activities. However, there is no information about the profile and content of phenolic compounds in Russian wines. We firstly analyzed phenolics (stilbenes, phenolic acids, and flavonols) in some representatives of Russian wines, including eleven red and seven white Russian wines from Fanagoria, Krasnodarsky Territory. The Russian red wines contained six stilbenes (trans-resveratrol, cis-resveratrol, trans-, cis-piceid, trans-piceatannol, δ-viniferin), while the white wines contained only five stilbenes (cis-resveratrol, trans-, cis-piceid, trans-piceatannol, trans-resveratrol). More than a half of the total stilbenes in the wines (65% of all stilbenes) were presented by trans-piceid and cis-piceid, while trans-resveratrol reached 16% of all the stilbenes. The red wines also contained six phenolic acids and six flavonols, while the white wines contained six phenolic acids and only three flavonols. Myrecitin-3-O-glucoside, quercetin-3-O-glucoside, and myricetin were the major flavonols in the red wines, while dihydroquercetin-3-O-rhamnoside was the major flavonol in the white wines. The red wines contained markedly higher amounts of stilbenes, phenolic acids, and flavonols than the white wines. Thus, the data showed that young red Russian Fanagoria wines represent a rich source of phenolic compounds. The study also revealed that younger wines were more abundant in phenolics, and wine storage for six months in the dark at +10 °C led to a decrease in the total content of phenolics, primarily monomeric stilbenes and quercetin-3-O-glucoside and quercetin flavonols.

ACS Style

Andrey Suprun; Alexandra Dubrovina; Alexey Tyunin; Konstantin Kiselev. Profile of Stilbenes and Other Phenolics in Fanagoria White and Red Russian Wines. Metabolites 2021, 11, 231 .

AMA Style

Andrey Suprun, Alexandra Dubrovina, Alexey Tyunin, Konstantin Kiselev. Profile of Stilbenes and Other Phenolics in Fanagoria White and Red Russian Wines. Metabolites. 2021; 11 (4):231.

Chicago/Turabian Style

Andrey Suprun; Alexandra Dubrovina; Alexey Tyunin; Konstantin Kiselev. 2021. "Profile of Stilbenes and Other Phenolics in Fanagoria White and Red Russian Wines." Metabolites 11, no. 4: 231.

Original article
Published: 06 March 2021 in Plant Cell, Tissue and Organ Culture (PCTOC)
Reads 0
Downloads 0

Calmodulins (CaMs) and calmodulin-like proteins (CMLs) are important plant Ca2+ sensor proteins implicated in the regulation of plant response to a variety of biotic and abiotic environmental cues. The knowledge on the relationship of calcium sensor proteins with plant defense responses and secondary metabolite production remains limited. The present study aimed for a comprehensive analysis of CaM and CML gene expression in a highly resistant grapevine Vitis amurensis Rupr., known as a reach stilbene source, in response to stress hormones (salicylic acid or SA, methyl jasmonate or MeJA), phenolic precursors (p-coumaric acids or CAs), and ultraviolet irradiation (UV-C). Using quantitative real-time PCR (qRT-PCR), we analyzed the expression of 3 VaCaM and 54 VaCML genes in the leaves and cell cultures of V. amurensis coupled to the activation of stilbene biosynthesis in response to the treatments. MeJa highly up-regulated 4 and 8 VaCML genes in the V. amurensis leaves and the cell cultures at both concentrations tested, respectively; while SA induced 4 and 6 VaCMLs. UV-C irradiation considerably up-regulated 4 and 9 VaCML genes in the leaves and cell cultures, respectively, both 1 h and 24 h post-treatment. At the same time, the MeJa, SA, and UV treatments exerted a profound down-regulation effect on 3 VaCML genes. VaCaM showed a lower responsiveness to the treatments, with irregular VaCaM8 and VaCaM10 transcriptional changes. CA treatment mostly did not affect VaCaM or VaCML expression. The highly up-regulated CaM and CML genes are suggested as promising candidates playing important roles in stress hormone signaling, UV-C responses, and stilbene biosynthesis.

ACS Style

Konstantin V. Kiselev; Olga A. Aleynova; Zlata V. Ogneva; Andrey R. Suprun; Alexey A. Ananev; Nikolay N. Nityagovsky; Alexandra S. Dubrovina. The effect of stress hormones, UV-C, and stilbene precursors on calmodulin (CaM) and calmodulin-like gene (CML) expression in Vitis amurensis Rupr. Plant Cell, Tissue and Organ Culture (PCTOC) 2021, 146, 59 -68.

AMA Style

Konstantin V. Kiselev, Olga A. Aleynova, Zlata V. Ogneva, Andrey R. Suprun, Alexey A. Ananev, Nikolay N. Nityagovsky, Alexandra S. Dubrovina. The effect of stress hormones, UV-C, and stilbene precursors on calmodulin (CaM) and calmodulin-like gene (CML) expression in Vitis amurensis Rupr. Plant Cell, Tissue and Organ Culture (PCTOC). 2021; 146 (1):59-68.

Chicago/Turabian Style

Konstantin V. Kiselev; Olga A. Aleynova; Zlata V. Ogneva; Andrey R. Suprun; Alexey A. Ananev; Nikolay N. Nityagovsky; Alexandra S. Dubrovina. 2021. "The effect of stress hormones, UV-C, and stilbene precursors on calmodulin (CaM) and calmodulin-like gene (CML) expression in Vitis amurensis Rupr." Plant Cell, Tissue and Organ Culture (PCTOC) 146, no. 1: 59-68.

Journal article
Published: 30 January 2021 in Plants
Reads 0
Downloads 0

: Recent studies have revealed that foliar application of double-stranded RNAs (dsRNAs) or small-interfering RNAs (siRNAs) encoding specific genes of plant pathogens triggered RNA interference (RNAi)-mediated silencing of the gene targets. However, a limited number of reports documented silencing of plant endogenes or transgenes after direct foliar RNA application. This study analyzed the importance of physiological conditions (plant age, time of day, soil moisture, high salinity, heat, and cold stresses) and different dsRNA application means (brush spreading, spraying, infiltration, inoculation, needle injection, and pipetting) for suppression of neomycin phosphotransferase II (NPTII) transgene in Arabidopsis thaliana, as transgenes are more prone to silencing. We observed a higher NPTII suppression when dsRNA was applied at late day period, being most efficient at night, which revealed a diurnal variation in dsRNA treatment efficacy. Exogenous NPTII-dsRNA considerably reduced NPTII expression in 4-week-old plants and only limited it in 2- and 6-week-old plants. In addition, a more discernible NPTII downregulation was detected under low soil moisture conditions. Treatment of adaxial and abaxial leaf surfaces by brushes, spraying, and pipetting showed a higher NPTII suppression, while infiltration and inoculation were less efficient. Thus, appropriate plant age, late time of day, low soil moisture, and optimal dsRNA application modes are important for exogenously induced gene silencing.

ACS Style

Konstantin V. Kiselev; Andrey R. Suprun; Olga A. Aleynova; Zlata V. Ogneva; Alexandra S. Dubrovina. Physiological Conditions and dsRNA Application Approaches for Exogenously induced RNA Interference in Arabidopsis thaliana. Plants 2021, 10, 264 .

AMA Style

Konstantin V. Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata V. Ogneva, Alexandra S. Dubrovina. Physiological Conditions and dsRNA Application Approaches for Exogenously induced RNA Interference in Arabidopsis thaliana. Plants. 2021; 10 (2):264.

Chicago/Turabian Style

Konstantin V. Kiselev; Andrey R. Suprun; Olga A. Aleynova; Zlata V. Ogneva; Alexandra S. Dubrovina. 2021. "Physiological Conditions and dsRNA Application Approaches for Exogenously induced RNA Interference in Arabidopsis thaliana." Plants 10, no. 2: 264.

Journal article
Published: 26 October 2020 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Calmodulin-like proteins (CMLs) represent a large family of plant calcium sensor proteins involved in the regulation of plant responses to environmental cues and developmental processes. In the present work, we identified four alternatively spliced mRNA forms of the grapevine CML21 gene that encoded proteins with distinct N-terminal regions. We studied the transcript abundance of CML21v1, CML21v2, CML21v3, and CML21v4 in wild-growing grapevine Vitis amurensis Rupr. in response to desiccation, heat, cold, high salinity, and high mannitol stress using quantitative real-time RT-PCR. The levels of all four splice variants of VaCML21 were highly induced in response to cold stress. In addition, VaCML21v1 and VaCML21v2 forms were highly modulated by all other abiotic stress treatments. Constitutive expression of VaCML21v2 and VaCML21v4 improved biomass accumulation of V. amurensis callus cell cultures under prolonged low temperature stress. Heterologous expression of the grapevine CML21v2 and VaCML21v4 splice variants in Arabidopsis improved survival rates of the transgenic plants after freezing. The VaCML21v2 overexpression enhanced activation of the cold stress-responsive marker genes AtDREB1A and AtDREB2A, while VaCML21v4 overexpression—AtCOR47, AtRD29A, AtRD29B, and AtKIN1 genes after freezing stress in the transgenic Arabidopsis. The results indicate that the grapevine CML21 gene acts as a positive regulator in the plant response to cold stress. The detected variety of CML21 transcripts and their distinct transcriptional responses suggested that this expansion of mRNA variants could contribute to the diversity of grapevine adaptive reactions.

ACS Style

Olga A. Aleynova; Konstantin V. Kiselev; Zlata V. Ogneva; Alexandra S. Dubrovina. The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response. International Journal of Molecular Sciences 2020, 21, 7939 .

AMA Style

Olga A. Aleynova, Konstantin V. Kiselev, Zlata V. Ogneva, Alexandra S. Dubrovina. The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response. International Journal of Molecular Sciences. 2020; 21 (21):7939.

Chicago/Turabian Style

Olga A. Aleynova; Konstantin V. Kiselev; Zlata V. Ogneva; Alexandra S. Dubrovina. 2020. "The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response." International Journal of Molecular Sciences 21, no. 21: 7939.

Review article
Published: 11 January 2020 in Biotechnology and Applied Biochemistry
Reads 0
Downloads 0

Plant stilbenes have attracted special attention, since they possess valuable health benefits and improve plant resistance to environmental stresses. Stilbenes are synthesized via the phenylpropanoid pathway, where stilbene synthase (STS, EC 2.3.1.95) directly catalyzes the formation of t‐resveratrol (monomeric stilbene). This review discusses the features of using STS genes in genetic engineering and plant biotechnology with the purpose to increase plant resistance to environmental stresses and to modify secondary metabolite production. This article is protected by copyright. All rights reserved

ACS Style

Konstantin V. Kiselev; Alexandra S. Dubrovina. Overexpression of stilbene synthase genes to modulate the properties of plants and plant cell cultures. Biotechnology and Applied Biochemistry 2020, 68, 13 -19.

AMA Style

Konstantin V. Kiselev, Alexandra S. Dubrovina. Overexpression of stilbene synthase genes to modulate the properties of plants and plant cell cultures. Biotechnology and Applied Biochemistry. 2020; 68 (1):13-19.

Chicago/Turabian Style

Konstantin V. Kiselev; Alexandra S. Dubrovina. 2020. "Overexpression of stilbene synthase genes to modulate the properties of plants and plant cell cultures." Biotechnology and Applied Biochemistry 68, no. 1: 13-19.

Journal article
Published: 13 December 2019 in Plants
Reads 0
Downloads 0

Plant calmodulins (CaMs) and calmodulin-like proteins (CMLs) are important plant Ca2+-binding proteins that sense and decode changes in the intracellular Ca2+ concentration arising in response to environmental stimuli. Protein Ca2+ sensors are presented by complex gene families in plants and perform diverse biological functions. In this study, we cloned, sequenced, and characterized three CaM and 54 CML mRNA transcripts of Vitis amurensis Rupr., a wild-growing grapevine with a remarkable stress tolerance. Using real-time quantitative RT-PCR, we analyzed transcript abundance of the identified VaCaMs and VaCMLs in response to water deficit, high salinity, high mannitol, cold and heat stresses. Expression of VaCaMs and 32 VaCMLs actively responded to the abiotic stresses and exhibited both positive and negative regulation patterns. Other VaCML members showed slight transcriptional regulation, remained essentially unresponsive or responded only after one time interval of the treatments. The substantial alterations in the VaCaM and VaCML transcript levels revealed their involvement in the adaptation of wild-growing grapevine to environmental stresses.

ACS Style

Alexandra S. Dubrovina; Olga A. Aleynova; Zlata V. Ogneva; Andrey R. Suprun; Alexey A. Ananev; Konstantin V. Kiselev. The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis. Plants 2019, 8, 602 .

AMA Style

Alexandra S. Dubrovina, Olga A. Aleynova, Zlata V. Ogneva, Andrey R. Suprun, Alexey A. Ananev, Konstantin V. Kiselev. The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis. Plants. 2019; 8 (12):602.

Chicago/Turabian Style

Alexandra S. Dubrovina; Olga A. Aleynova; Zlata V. Ogneva; Andrey R. Suprun; Alexey A. Ananev; Konstantin V. Kiselev. 2019. "The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis." Plants 8, no. 12: 602.

Original article
Published: 17 October 2019 in Biotechnology and Applied Biochemistry
Reads 0
Downloads 0

Stilbenes are natural compounds protecting plants against microbial pathogens and known to possess valuable biologically active properties. In the present study, we established transgenic grapevine callus cell cultures overexpressing three stilbene synthase (STS) genes of spruce Picea jezoensis PjSTS1a, PjSTS2, and PjSTS3. Transformation of Vitis amurensis calli with the PjSTS1a, PjSTS2, and PjSTS3 genes significantly increased total content of stilbenes in 3.6-6, 2.5-2.9, and 4.1-16.1 times, respectively, in comparison with the control calli. The most pronounced positive effect on the accumulation of stilbenes was observed for the PjSTS3-overexpressing calli where the total content of stilbenes was increased up to 3.1 mg/g DW, and the stilbene production reached 25.4 mg/L. These values were higher than those achieved for the grapevine callus cell cultures overexpressing three STS genes from V. amurensis. Thus, transformation of grapevine cell cultures with spruce STS genes with a relatively low degree of homology to the endogenous VaSTSs is a more effective strategy for induction of plant secondary metabolite biosynthesis than using the grapevine genes for the overexpression experiments.

ACS Style

Andrey Suprun; Z. V. Ogneva; A. S. Dubrovina; K. V. Kiselev. Effect of spruce PjSTS1a , PjSTS2 , or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr. Biotechnology and Applied Biochemistry 2019, 67, 234 -239.

AMA Style

Andrey Suprun, Z. V. Ogneva, A. S. Dubrovina, K. V. Kiselev. Effect of spruce PjSTS1a , PjSTS2 , or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr. Biotechnology and Applied Biochemistry. 2019; 67 (2):234-239.

Chicago/Turabian Style

Andrey Suprun; Z. V. Ogneva; A. S. Dubrovina; K. V. Kiselev. 2019. "Effect of spruce PjSTS1a , PjSTS2 , or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr." Biotechnology and Applied Biochemistry 67, no. 2: 234-239.

Review
Published: 08 May 2019 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Recent investigations documented that plants can uptake and process externally applied double-stranded RNAs (dsRNAs), hairpin RNAs (hpRNAs), and small interfering RNAs (siRNAs) designed to silence important genes of plant pathogenic viruses, fungi, or insects. The exogenously applied RNAs spread locally and systemically, move into the pathogens, and induce RNA interference-mediated plant pathogen resistance. Recent findings also provided examples of plant transgene and endogene post-transcriptional down-regulation by complementary dsRNAs or siRNAs applied onto the plant surfaces. Understanding the plant perception and processing of exogenous RNAs could result in the development of novel biotechnological approaches for crop protection. This review summarizes and discusses the emerging studies reporting on exogenous RNA applications for down-regulation of essential fungal and insect genes, targeting of plant viruses, or suppression of plant transgenes and endogenes for increased resistance and changed phenotypes. We also analyze the current understanding of dsRNA uptake mechanisms and dsRNA stability in plant environments.

ACS Style

Alexandra S. Dubrovina; Konstantin V. Kiselev. Exogenous RNAs for Gene Regulation and Plant Resistance. International Journal of Molecular Sciences 2019, 20, 2282 .

AMA Style

Alexandra S. Dubrovina, Konstantin V. Kiselev. Exogenous RNAs for Gene Regulation and Plant Resistance. International Journal of Molecular Sciences. 2019; 20 (9):2282.

Chicago/Turabian Style

Alexandra S. Dubrovina; Konstantin V. Kiselev. 2019. "Exogenous RNAs for Gene Regulation and Plant Resistance." International Journal of Molecular Sciences 20, no. 9: 2282.

Journal article
Published: 29 March 2019 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Recent investigations show that exogenously applied small interfering RNAs (siRNA) and long double-stranded RNA (dsRNA) precursors can be taken up and translocated in plants to induce RNA interference (RNAi) in the plant or in its fungal pathogen. The question of whether genes in the plant genome can undergo suppression as a result of exogenous RNA application on plant surface is almost unexplored. This study analyzed whether it is possible to influence transcript levels of transgenes, as more prone sequences to silencing, in Arabidopsis genome by direct exogenous application of target long dsRNAs. The data revealed that in vitro synthesized dsRNAs designed to target the gene coding regions of enhanced green fluorescent protein (EGFP) or neomycin phosphotransferase II (NPTII) suppressed their transcript levels in Arabidopsis. The fact that, simple exogenous application of polynucleotides can affect mRNA levels of plant transgenes, opens new opportunities for the development of new scientific techniques and crop improvement strategies.

ACS Style

Alexandra S. Dubrovina; Olga A. Aleynova; Alexander V. Kalachev; Andrey Suprun; Zlata V. Ogneva; Konstantin V. Kiselev. Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA. International Journal of Molecular Sciences 2019, 20, 1585 .

AMA Style

Alexandra S. Dubrovina, Olga A. Aleynova, Alexander V. Kalachev, Andrey Suprun, Zlata V. Ogneva, Konstantin V. Kiselev. Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA. International Journal of Molecular Sciences. 2019; 20 (7):1585.

Chicago/Turabian Style

Alexandra S. Dubrovina; Olga A. Aleynova; Alexander V. Kalachev; Andrey Suprun; Zlata V. Ogneva; Konstantin V. Kiselev. 2019. "Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA." International Journal of Molecular Sciences 20, no. 7: 1585.

Journal article
Published: 01 March 2019 in Russian Journal of Genetics
Reads 0
Downloads 0

Calcium-dependent protein kinases (CDPK or CPK) are involved in protecting plants from abiotic stresses, but the properties and functions of CDPK are still poorly understood. Analysis of the Amur grape Vitis amurensis Rupr. is of great interest, since it demonstrates high tolerance to adverse environmental conditions. It was demonstrated that exposure of V. amurensis to salt and, to a lesser extent, osmotic and cold stress resulted in a considerable increase in the VaCPK1 and VaCPK26 transcription level. The present study was focused on the analysis of the effect of constitutive expression of the recombinant VaCPK1 and VaCPK26 genes on the resistance of V. amurensis cell cultures and Arabidopsis thaliana plants to salt, osmotic, and temperature stresses. It was demonstrated that expression of recombinant VaCPK26, as well as to a lesser extent VaCPK1, led to a 1.2–1.7 times increase in biomass accumulation by V. amurensis cell cultures and to a 1.2–2.1 times increase in survival of A. thaliana plants under salt stress. In addition, VaCPK26-expressing lines of A. thaliana lines demonstrated a slight degree of drought tolerance and were characterized by increased expression of some stress-induced protective genes involved in the formation of salt and dehydration tolerance. The data obtained indicate that the VaCPK1 and VaCPK26 genes can act as positive regulators of plant response to salt stress.

ACS Style

A. S. Dubrovina; K. V. Kiselev. The Role of Calcium-Dependent Protein Kinase Genes VaCPK1 and VaCPK26 in the Response of Vitis amurensis (in vitro) and Arabidopsis thaliana (in vivo) to Abiotic Stresses. Russian Journal of Genetics 2019, 55, 319 -329.

AMA Style

A. S. Dubrovina, K. V. Kiselev. The Role of Calcium-Dependent Protein Kinase Genes VaCPK1 and VaCPK26 in the Response of Vitis amurensis (in vitro) and Arabidopsis thaliana (in vivo) to Abiotic Stresses. Russian Journal of Genetics. 2019; 55 (3):319-329.

Chicago/Turabian Style

A. S. Dubrovina; K. V. Kiselev. 2019. "The Role of Calcium-Dependent Protein Kinase Genes VaCPK1 and VaCPK26 in the Response of Vitis amurensis (in vitro) and Arabidopsis thaliana (in vivo) to Abiotic Stresses." Russian Journal of Genetics 55, no. 3: 319-329.

Short communication
Published: 14 February 2019 in Journal of Plant Physiology
Reads 0
Downloads 0

This study examines the effects of p-coumaric (CoA) and caffeic (CaA) acids on stilbene biosynthesis in one-year-old needles of Picea jezoensis (Lindl. et Gord.) Fisch ex Carr. under control conditions and after ultraviolet (UV-C) irradiation. HPLC analysis revealed that while UV-C irradiation slightly affected the total stilbene content, CoA and CaA feeding increased the total content of stilbenes by 1.2-1.3-fold. UV-C treatment combined with CoA-feeding of the P. jezoensis cuttings exerted the most pronounced stimulatory effect on stilbene accumulation leading to the total stilbene content of 9.18 mg/g of dry weight or DW. This increase correlated with the elevated transcription of the stilbene synthase PjSTS1a and PjSTS1b genes. UV-C treatment in combination with CaA feeding of P. jezoensis did not considerably influence stilbene content. These results revealed a positive effect of UV-C radiation and phenolic precursors on the content of stilbenes in spruce needles.

ACS Style

K.V. Kiselev; V.P. Grigorchuk; Z.V. Ogneva; Andrey Suprun; A.S. Dubrovina. The effect of ultraviolet-C and precursor feeding on stilbene biosynthesis in spruce Picea jezoensis. Journal of Plant Physiology 2019, 234-235, 133 -137.

AMA Style

K.V. Kiselev, V.P. Grigorchuk, Z.V. Ogneva, Andrey Suprun, A.S. Dubrovina. The effect of ultraviolet-C and precursor feeding on stilbene biosynthesis in spruce Picea jezoensis. Journal of Plant Physiology. 2019; 234-235 ():133-137.

Chicago/Turabian Style

K.V. Kiselev; V.P. Grigorchuk; Z.V. Ogneva; Andrey Suprun; A.S. Dubrovina. 2019. "The effect of ultraviolet-C and precursor feeding on stilbene biosynthesis in spruce Picea jezoensis." Journal of Plant Physiology 234-235, no. : 133-137.

Journal article
Published: 11 February 2019 in Biologia plantarum
Reads 0
Downloads 0

An inhibitor of DNA methylation 5-azacytidine (5A) is a chemical analog of the nucleoside cytidine. This study investigated the influence of 5A-induced DNA hypomethylation on the accumulation of somatic DNA mutations (nucleotide substitutions,...

ACS Style

K.V. Kiselev; Z.V. Ogneva; A.S. Dubrovina; N.N. Nityagovsky; Andrey Suprun. Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine. Biologia plantarum 2019, 63, 398 -404.

AMA Style

K.V. Kiselev, Z.V. Ogneva, A.S. Dubrovina, N.N. Nityagovsky, Andrey Suprun. Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine. Biologia plantarum. 2019; 63 (1):398-404.

Chicago/Turabian Style

K.V. Kiselev; Z.V. Ogneva; A.S. Dubrovina; N.N. Nityagovsky; Andrey Suprun. 2019. "Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine." Biologia plantarum 63, no. 1: 398-404.

Short communication
Published: 04 February 2019 in Acta Physiologiae Plantarum
Reads 0
Downloads 0

This paper investigated the effects of p-coumaric acid (CA) on stilbene biosynthesis in healthy cuttings of grapevine Vitis amurensis Rupr. under control conditions and after ultraviolet (UV-C) irradiation. It has been shown that the content of the detected six stilbenes (cis-piceid, t-piceid, t-ε-viniferin, cis-ε-viniferin, t-resveratrol, and t-δ-viniferin) increased after feeding with CA up to 0.5 mg g−1 of the dry weight (DW), which is 2.4 times higher than that under control conditions. Feeding with CA and UV-C irradiation exerted a combined positive effect on the content of stilbenes increasing it to 0.7 mg g−1 DW. In addition, the CA treatment improved viability of the cuttings after the UV-C treatment. The elevation in stilbene content induced by CA and UV-C correlated with induction of stilbene biosynthetic genes encoding stilbene synthases, resveratrol O-glucosyltransferase, polyphenol oxidase, and cationic peroxidase. The data indicate that feeding with the precursors of phenolic compounds could be an effective strategy for activation of stilbene production.

ACS Style

Konstantin V. Kiselev; Zlata V. Ogneva; Andrey Suprun; Valeria P. Grigorchuk; Alexandra S. Dubrovina. Action of ultraviolet-C radiation and p-coumaric acid on stilbene accumulation and expression of stilbene biosynthesis-related genes in the grapevine Vitis amurensis Rupr. Acta Physiologiae Plantarum 2019, 41, 28 .

AMA Style

Konstantin V. Kiselev, Zlata V. Ogneva, Andrey Suprun, Valeria P. Grigorchuk, Alexandra S. Dubrovina. Action of ultraviolet-C radiation and p-coumaric acid on stilbene accumulation and expression of stilbene biosynthesis-related genes in the grapevine Vitis amurensis Rupr. Acta Physiologiae Plantarum. 2019; 41 (2):28.

Chicago/Turabian Style

Konstantin V. Kiselev; Zlata V. Ogneva; Andrey Suprun; Valeria P. Grigorchuk; Alexandra S. Dubrovina. 2019. "Action of ultraviolet-C radiation and p-coumaric acid on stilbene accumulation and expression of stilbene biosynthesis-related genes in the grapevine Vitis amurensis Rupr." Acta Physiologiae Plantarum 41, no. 2: 28.

Original research paper
Published: 02 January 2019 in Biotechnology Letters
Reads 0
Downloads 0

Studies concerning 2b protein from Cucumovirus showed 2b to effectively repress functioning of the plant silencing complex, current study aimed whether retransformation with 2b gene able to restore silenced transgene expression in plant cells. A rolB-transgenic cell culture of Vitis amurensis Rupr. that was continuously subcultured during more than 10 years and exhibited decreased transcription of the rolB transgene was retransformed with the 2b gene of Cucumovirus-NK. Three cell lines retransformed with 2b showed a significant up-regulation of rolB expression accompanied with enhancements in their stilbenes content level in more than 2,7-fold compared to parental rolB-transgenic cell line. The mentioned increase in the level of stilbenes content was due to activation of certain stilbene synthase genes expression responsible for stilbenes biosynthesis in V. amurensis cells. Restoration of rolB expression upon 2b-retransformation led to increase in the expression levels of VaSTS2-VaSTS5 and VaSTS7 isoforms. 2b from CMV-NK can reactivate a silenced transgene expression, even after 10 years of subcultivation, nevertheless, optimization of the methods concerning 2b introduction in plant genomes is necessary to avoid undesirable silencing effects.

ACS Style

Alexey P. Tyunin; Nikolay N. Nityagovsky; Yuri A. Karetin; Konstantin V. Kiselev. Reactivation of rolB transgene expression in Vitis amurensis Rupr. cells upon retransformation with 2b gene from Cucumovirus isolate NK. Biotechnology Letters 2019, 41, 443 -451.

AMA Style

Alexey P. Tyunin, Nikolay N. Nityagovsky, Yuri A. Karetin, Konstantin V. Kiselev. Reactivation of rolB transgene expression in Vitis amurensis Rupr. cells upon retransformation with 2b gene from Cucumovirus isolate NK. Biotechnology Letters. 2019; 41 (3):443-451.

Chicago/Turabian Style

Alexey P. Tyunin; Nikolay N. Nityagovsky; Yuri A. Karetin; Konstantin V. Kiselev. 2019. "Reactivation of rolB transgene expression in Vitis amurensis Rupr. cells upon retransformation with 2b gene from Cucumovirus isolate NK." Biotechnology Letters 41, no. 3: 443-451.

Short communication
Published: 21 December 2018 in Tissue and Cell
Reads 0
Downloads 0

An ultrastructural study of developing spermatids in sea urchins, Strongylocentrotus intermedius, showed that macroautophagy is involved in formation of residual bodies and removal of excessive cytoplasm by spermatids during spermatogenesis in this species. During late stages of spermatogenesis spermatids sequester excessive cytoplasm into vesicles, surrounded by a double membrane. Subsequently, these vesicles fused to one another into larger vacuoles, up to 1.5 μm in diameter. Finally, the vacuoles transformed into residual bodies by condensing their content into finely granular material of varying electron density, separated from cytoplasm by a single membrane. An immunoelectron microscopic study of late spermatids with the antibodies, raised against microtubule-associated protein 1 A/1B-light chain 3 (LC3), which is a marker of autophagosomes, showed that residual bodies in late spermatids of S. intermedius were LC3-positive.

ACS Style

Alexander V. Kalachev; Olga V. Yurchenko; Konstantin V. Kiselev. Macroautophagy is involved in residual bodies formation during spermatogenesis in sea urchins, Strongylocentrotus intermedius. Tissue and Cell 2018, 56, 79 -82.

AMA Style

Alexander V. Kalachev, Olga V. Yurchenko, Konstantin V. Kiselev. Macroautophagy is involved in residual bodies formation during spermatogenesis in sea urchins, Strongylocentrotus intermedius. Tissue and Cell. 2018; 56 ():79-82.

Chicago/Turabian Style

Alexander V. Kalachev; Olga V. Yurchenko; Konstantin V. Kiselev. 2018. "Macroautophagy is involved in residual bodies formation during spermatogenesis in sea urchins, Strongylocentrotus intermedius." Tissue and Cell 56, no. : 79-82.

Research note
Published: 10 September 2018 in Plant Cell, Tissue and Organ Culture (PCTOC)
Reads 0
Downloads 0

Plant cell and tissue cultures are considered as a source of valuable secondary metabolites but usually produce insufficient level of the compounds, which is the limiting factor for their application in biotechnology. We obtained 18 callus cell cultures from different organs of wild grape Vitis amurensis Rupr. collected at different seasons and analyzed stilbene accumulation in combination with calli growth parameters. This analysis showed that temporal and tissue origin of the calli affected the rate of stilbene biosynthesis. Stem-derived calli accumulated higher stilbene levels and exhibited a higher expression of phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) genes than calli derived from the leaves and petioles. The highest content of stilbenes was detected in the calli initiated from grapevine stems collected in the autumn. In general, all “autumn” cell cultures contained more than 2 mg g− 1 dry wt (up to 11 mg g− 1 dry wt) and exhibited high PAL and STS genes expression in comparison with the calli initiated in the summer. The content of stilbenes in the “autumn” cell cultures were comparable to the highest stilbene contents detected in other plant sources described in the literature. Thus, selecting the most optimal explant source for cell culture establishment could be an effective approach towards developing plant cell cultures producing high stilbene levels.

ACS Style

A. P. Tyunin; Andrey Suprun; N. N. Nityagovsky; A. Y. Manyakhin; Y. A. Karetin; A. S. Dubrovina; K. V. Kiselev. The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr. Plant Cell, Tissue and Organ Culture (PCTOC) 2018, 136, 189 -196.

AMA Style

A. P. Tyunin, Andrey Suprun, N. N. Nityagovsky, A. Y. Manyakhin, Y. A. Karetin, A. S. Dubrovina, K. V. Kiselev. The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr. Plant Cell, Tissue and Organ Culture (PCTOC). 2018; 136 (1):189-196.

Chicago/Turabian Style

A. P. Tyunin; Andrey Suprun; N. N. Nityagovsky; A. Y. Manyakhin; Y. A. Karetin; A. S. Dubrovina; K. V. Kiselev. 2018. "The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr." Plant Cell, Tissue and Organ Culture (PCTOC) 136, no. 1: 189-196.

Journal article
Published: 13 July 2018 in Applied Biochemistry and Microbiology
Reads 0
Downloads 0

It is known that calcium-dependent protein kinases (CDPK or CPK) are implicated in the regulation of plant development and stress adaptation. However, there is a lack of information on the properties and functions of certain CDPK family members. The present study investigates the functions of four CDPK genes of the grapevine Vitis amurensis Rupr. in the formation of its high stress resistance level and the production of valuable secondary metabolites. Overexpression of the CPK30 gene of V. amurensis considerably increased the resistance of V. amurensis transgenic cell lines to salt and cold stresses, while CPK16, CPK25, and CPK32 overexpression did not influence the salt and temperature stress tolerance. VaCPK16 and VaCPK32 overexpression increased stilbene production in V. amurensis cell cultures by 2.1–3.1 and 1.6–3.1 times, respectively. The data indicate that the VaCPK30 gene is involved in the formation of grapevine salt and cold stress resistance, while the VaCPK16 and VaCPK32 genes contribute to increased stilbene accumulation.

ACS Style

A. S. Dubrovina; O. A. Aleynova; A. Y. Manyakhin; K. V. Kiselev. The Role of Calcium-Dependent Protein Kinase Genes CPK16, CPK25, CPK30, and CPK32 in Stilbene Biosynthesis and the Stress Resistance of Grapevine Vitis amurensis Rupr. Applied Biochemistry and Microbiology 2018, 54, 410 -417.

AMA Style

A. S. Dubrovina, O. A. Aleynova, A. Y. Manyakhin, K. V. Kiselev. The Role of Calcium-Dependent Protein Kinase Genes CPK16, CPK25, CPK30, and CPK32 in Stilbene Biosynthesis and the Stress Resistance of Grapevine Vitis amurensis Rupr. Applied Biochemistry and Microbiology. 2018; 54 (4):410-417.

Chicago/Turabian Style

A. S. Dubrovina; O. A. Aleynova; A. Y. Manyakhin; K. V. Kiselev. 2018. "The Role of Calcium-Dependent Protein Kinase Genes CPK16, CPK25, CPK30, and CPK32 in Stilbene Biosynthesis and the Stress Resistance of Grapevine Vitis amurensis Rupr." Applied Biochemistry and Microbiology 54, no. 4: 410-417.