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Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established.
Ji-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants 2020, 9, 688 .
AMA StyleJi-Sun Park, Zuh-Kyung Seong, Mi-Sun Kim, Jang-Ho Ha, Ki-Beom Moon, Hyo-Jun Lee, Hyeong-Kyu Lee, Jae-Heung Jeon, Sang Un Park, Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants. 2020; 9 (6):688.
Chicago/Turabian StyleJi-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. 2020. "Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton." Plants 9, no. 6: 688.
A fast and reliable ultra-performance liquid chromatography-diode array detection method was developed and validated for the quantitative assessment of turmeric extracts from different geographical locations. Acclaim RSLC PolarAdvantage II column (2.2 μm, 2.1 × 100 mm) was used to analyze individual curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) from turmeric samples. The detection was done on ultraviolet absorbance at 425 nm and the column temperature was maintained at 45 °C. A mobile phase consisting of acetonitrile and water was found to be suitable for separation, at a flow rate of 1 mL/min with linear gradient elution. Linearity, specificity, precision, recovery and robustness were measured to validate the method and instrument. Under the described conditions, curcuminoids were collected within one minute. The calibration curve of each curcuminoid showed good linearity (correlation coefficient > 0.999). The relative standard deviations (RSD) of intra-day, inter-day precision and repeatability were less than 0.73%, 2.47% and 2.47%, respectively. In the recovery test, the accuracy ranged from 98.54%-103.91% with RSD values of less than 2.79%. The developed method was used for quantification of individual curcuminoids of turmeric samples. Analysis of turmeric samples from Nepal and South Korea revealed that curcuminoid content was related to geographical location. Turmeric cultivated in warmer climates were found to have higher curcumionoid content than turmeric samples from cooler climates, the southern part of Nepal was found to have two times higher content of curcuminoids than turmeric from the north.
Amrit Poudel; Jitendra Pandey; Hyeong-Kyu Lee. Geographical Discrimination in Curcuminoids Content of Turmeric Assessed by Rapid UPLC-DAD Validated Analytical Method. Molecules 2019, 24, 1805 .
AMA StyleAmrit Poudel, Jitendra Pandey, Hyeong-Kyu Lee. Geographical Discrimination in Curcuminoids Content of Turmeric Assessed by Rapid UPLC-DAD Validated Analytical Method. Molecules. 2019; 24 (9):1805.
Chicago/Turabian StyleAmrit Poudel; Jitendra Pandey; Hyeong-Kyu Lee. 2019. "Geographical Discrimination in Curcuminoids Content of Turmeric Assessed by Rapid UPLC-DAD Validated Analytical Method." Molecules 24, no. 9: 1805.
Royal jelly (RJ) has been used traditionally for dietary, cosmetic and health purposes for a long time in different parts of the world. Scientific studies have also shown its numerous health-promoting properties including hypoglycemic and anti-hypercholesterolemic action. In this study, we investigated the anti-adipogenic activity of RJ in 3 T3-L1 cells and isolated the major responsible root component for the activity. An active anti-adipogenic compound was isolated through bioassay-guided isolation process by successive treatment of RJ and its active fractions on 3 T3-L1 cell line. (E)-10-Hydroxy-2-decenoic Acid (10-HDA) was identified using NMR spectroscopy and ultra-performance liquid chromatography (UPLC). As 10-HDA showed significant anti-adipogenic activity with Oil Red O staining and TG content assay on 3 T3-L1 adipocytes, further study was carried out in molecular level for the expression of adipogenic transcription factors such as PPARγ, FABP4, C/EBPα, SREBP-1c, and Leptin. The effect of 10-HDA on preliminary molecules such as pAkt, pERK, C/EBPβ, and pCREB were studied in the early stage of adipogenesis. The effect of 10-HDA on reactive oxygen species (ROS) production in fully differentiating adipocytes was measured by nitro blue tetrazolium (NBT) assay. Results showed that triacylglycerol accumulation and ROS production was markedly suppressed by 10-HDA. Preliminary molecules such as pAkt, pERK, pCERB, and C/EBPβ were found to be down-regulated by 10-HDA, which led to down-regulation of key adipogenic transcription factors such as PPARγ, FABP4, CEBPα, SREBP-1c, and Leptin on 3 T3-L1 adipocytes. Our results suggest that anti-adipogenesis of 10-HDA on 3 T3-L1 adipocyte takes place via two mechanisms: inhibition of cAMP/PKA pathway and inhibition of p-Akt and MAPK dependent insulin signaling pathway. So it is considered that 10-HDA, a major component of RJ, can be a potential therapeutic medicine for obesity.
Prakash Raj Pandeya; Ramakanta Lamichhane; Kyung-Hee Lee; Se-Gun Kim; Dae-Ho Lee; Hyeong-Kyu Lee; Hyun-Ju Jung. Bioassay-guided isolation of active anti-adipogenic compound from royal jelly and the study of possible mechanisms. BMC Complementary and Alternative Medicine 2019, 19, 1 -14.
AMA StylePrakash Raj Pandeya, Ramakanta Lamichhane, Kyung-Hee Lee, Se-Gun Kim, Dae-Ho Lee, Hyeong-Kyu Lee, Hyun-Ju Jung. Bioassay-guided isolation of active anti-adipogenic compound from royal jelly and the study of possible mechanisms. BMC Complementary and Alternative Medicine. 2019; 19 (1):1-14.
Chicago/Turabian StylePrakash Raj Pandeya; Ramakanta Lamichhane; Kyung-Hee Lee; Se-Gun Kim; Dae-Ho Lee; Hyeong-Kyu Lee; Hyun-Ju Jung. 2019. "Bioassay-guided isolation of active anti-adipogenic compound from royal jelly and the study of possible mechanisms." BMC Complementary and Alternative Medicine 19, no. 1: 1-14.
Melanin plays an important role in protecting the skin against ultraviolet light and is responsible for skin color. However, overproduction of melanin is related to several skin disorders, such as age spots, freckles, café au lait spots, Becker’s nevus and other hyperpigmentation syndromes. The aim of this study was to identify the effects of kaempferol-7-O-β-d-glucuronide (K7G) and tilianin, isolated from Cryptotaenia japonica, on melanogenesis and their mechanisms of action in murine B16 melanoma cells. The α-melanocyte-stimulating hormone (α-MSH)-induced melanin production was significantly inhibited by K7G and tilianin in a dose-dependent manner. The effects of these compounds on the signaling pathway of melanogenesis were examined. K7G and tilianin downregulated the expression of microphthalmia-associated transcription factor (MITF) and melanocyte-specific enzymes, i.e., tyrosinase and TRP1. These compounds also inhibited the phosphorylation of cyclic adenosine monophosphate (cAMP)-response element binding protein (CREB) in a dose-dependent manner. In addition, these compounds increased the phosphorylation of extracellular signal-regulated kinase (ERK) but decreased the phosphorylation of c-Jun N-terminal kinase (JNK) in B16 cells. Based on the above results, the anti-melanogenic effects of these compounds are caused by suppression of the MAPK signaling pathway through the down-regulation of α-MSH-induced CREB accumulation. This finding suggests that K7G and tilianin may be good candidates for further research to develop therapeutic agents for hyperpigmentation diseases.
Zuh-Kyung Seong; Sung-Yoon Lee; Amrit Poudel; Sei-Ryang Oh; Hyeong-Kyu Lee. Constituents of Cryptotaenia japonica Inhibit Melanogenesis via CREB- and MAPK-Associated Signaling Pathways in Murine B16 Melanoma Cells. Molecules 2016, 21, 1296 .
AMA StyleZuh-Kyung Seong, Sung-Yoon Lee, Amrit Poudel, Sei-Ryang Oh, Hyeong-Kyu Lee. Constituents of Cryptotaenia japonica Inhibit Melanogenesis via CREB- and MAPK-Associated Signaling Pathways in Murine B16 Melanoma Cells. Molecules. 2016; 21 (10):1296.
Chicago/Turabian StyleZuh-Kyung Seong; Sung-Yoon Lee; Amrit Poudel; Sei-Ryang Oh; Hyeong-Kyu Lee. 2016. "Constituents of Cryptotaenia japonica Inhibit Melanogenesis via CREB- and MAPK-Associated Signaling Pathways in Murine B16 Melanoma Cells." Molecules 21, no. 10: 1296.
Three new phenylacylphenol derivatives, stewartianol (1), deoxystewartianol-4′-O-arabinoglucoside (2), and stewartianol-3-O-glucoside (3), along with nine known compounds, methylesculin (4), fraxoside (5), fraxetin (6), scopletin (7), (+)-dihydromyricetin (8), (+)-taxifolin-7-O-β-d-glucose (9), (+)-taxifolin (10), (+)-dihydrokaempferol-7-O-β-d-glucose (11), and 3-acetyl-ursolic acid (12), were isolated from the twigs of Stewartia pseudocamellia; commonly used as folk medicine in Korea. The structures of the isolated compounds were identified using spectroscopic analysis, including 1D, 2D NMR, MS and compared with published data. The compounds were tested for their anti-melanogenic activity in cultured murine B16 melanoma cells. Stewartianol (1) and stewartianol-3-O-glucoside (3) showed an inhibitory effect significantly on melanogenesis in a concentration-dependent manner.
Zuh-Kyung Seong; Hui-Seong Kim; Yu-Mi Won; Jae-Lim Kim; Hyuk-Hwan Song; Doo-Young Kim; Sei-Ryang Oh; Hyun-Woo Cho; Jung-Hee Cho; Hyeong-Kyu Lee. Phenylacylphenol derivatives with anti-melanogenic activity from Stewartia pseudocamellia. Archives of Pharmacal Research 2016, 39, 636 -645.
AMA StyleZuh-Kyung Seong, Hui-Seong Kim, Yu-Mi Won, Jae-Lim Kim, Hyuk-Hwan Song, Doo-Young Kim, Sei-Ryang Oh, Hyun-Woo Cho, Jung-Hee Cho, Hyeong-Kyu Lee. Phenylacylphenol derivatives with anti-melanogenic activity from Stewartia pseudocamellia. Archives of Pharmacal Research. 2016; 39 (5):636-645.
Chicago/Turabian StyleZuh-Kyung Seong; Hui-Seong Kim; Yu-Mi Won; Jae-Lim Kim; Hyuk-Hwan Song; Doo-Young Kim; Sei-Ryang Oh; Hyun-Woo Cho; Jung-Hee Cho; Hyeong-Kyu Lee. 2016. "Phenylacylphenol derivatives with anti-melanogenic activity from Stewartia pseudocamellia." Archives of Pharmacal Research 39, no. 5: 636-645.
Kefir is a microbial symbiont mixture that produces jelly-like grains. As a widely used neutraceutical, however, the therapeutic applicabilityof kefir is not certain. In order to investigate the pharmacological effects of kefir, we used a mouse asthma model, in which airway inflammation and airway remodeling was produced by ovalbumin sensitization and challenge. BALB/c mice sensitized and challenged to ovalbumin, were treated with kefir (50 mg/kg administered by intra-gastric mode) 1 h before the ovalbumin challenge. Kefir significantly suppressed ovalbumin-induced airway hyper-responsiveness (AHR) to inhaled methacholine. Intra-gastric administration of kefir significantly inhibited the increase in the total inflammatory cell count induced by ovalbumin, and the eosinophil count in bronchoalveolar lavage fluid (BALF). Type 2 helper T cell (Th2) cytokines, such as interleukin-4 and interleukin-13, and total immunoglobulin E (Ig E) levels, were also reduced to normal levels in bronchoalveolar lavage fluid. Histological studies demonstrate that kefir substantially inhibited ovalbumin-induced eosinophilia in lung tissue and mucus hyper-secretion by goblet cells in the airway. Kefir displayed anti-inflammatory and anti-allergic effects in a mouse asthma model and may possess new therapeutic potential for the treatment of allergic bronchial asthma.
Mee-Young Lee; Kyung-Seop Ahn; Ok-Kyung Kwon; M Kim; In-Young Lee; Sei-Ryang Oh; Hyeong-Kyu Lee. Anti-inflammatory and anti-allergic effects of kefir in a mouse asthma model. Immunobiology 2007, 212, 647 -654.
AMA StyleMee-Young Lee, Kyung-Seop Ahn, Ok-Kyung Kwon, M Kim, In-Young Lee, Sei-Ryang Oh, Hyeong-Kyu Lee. Anti-inflammatory and anti-allergic effects of kefir in a mouse asthma model. Immunobiology. 2007; 212 (8):647-654.
Chicago/Turabian StyleMee-Young Lee; Kyung-Seop Ahn; Ok-Kyung Kwon; M Kim; In-Young Lee; Sei-Ryang Oh; Hyeong-Kyu Lee. 2007. "Anti-inflammatory and anti-allergic effects of kefir in a mouse asthma model." Immunobiology 212, no. 8: 647-654.
The EtOAc-soluble fraction from a methanolic extract ofHovenia dulcis Thunb. exhibited neuroprotective activity against glutamate-induced neurotoxicity in mouse hippocampal HT22 cells. The neuroprotective activity-guided isolation resulted in 8 phenolic compounds (1–8), such as vanillic acid (1), ferulic acid (2), 3,5-dihydroxystilbene (3), (+)-aromadendrin (4), methyl vanillate (5), (-)-catechin (6), 2,3,4-trihydrobenzoic acid (7), and (+)-afzelechin (8). Among these, compounds6 and8 had a neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells. Furthermore, compound6 had a DPPH free radical scavenging effect with an IC50 value of 57.7 μM, and a superoxide anion radical scavenging effect with an 8.0 μM. Both compounds6 and8 had ABTS cation radical scavenging effects with IC50 values of 7.8 μM and 23.7 μM, respectively. These results suggest that compounds6 and8 could be neuroprotectants owing to their free radical scavenging activities.
Gao Li; Byung-Sun Min; Changji Zheng; Joongku Lee; Sei-Ryang Oh; Kyung-Seop Ahn; Hyeong-Kyu Lee. Neuroprotective and free radical scavenging activities of phenolic compounds fromHovenia dulcis. Archives of Pharmacal Research 2005, 28, 804 -809.
AMA StyleGao Li, Byung-Sun Min, Changji Zheng, Joongku Lee, Sei-Ryang Oh, Kyung-Seop Ahn, Hyeong-Kyu Lee. Neuroprotective and free radical scavenging activities of phenolic compounds fromHovenia dulcis. Archives of Pharmacal Research. 2005; 28 (7):804-809.
Chicago/Turabian StyleGao Li; Byung-Sun Min; Changji Zheng; Joongku Lee; Sei-Ryang Oh; Kyung-Seop Ahn; Hyeong-Kyu Lee. 2005. "Neuroprotective and free radical scavenging activities of phenolic compounds fromHovenia dulcis." Archives of Pharmacal Research 28, no. 7: 804-809.
Dendropanax morbifera Leveille (Araliaceae) is used in Korea for a variety of disease, such as migraine headache and dysmenorrheal. A new polyacetylene (1) and six known compounds (2–7) were isolated from the leaves of this plant by conventional chromatographic techniques. The structure of the new polyacetylene (1) was determined as (9Z,16S)-16-hydroxy-9,17-octadecadiene-12,14-diynoic acid by spectroscopic means including 2D NMR, which comprised the determination of a chiral by modified Mosher’s ester method. Compounds 1–7 were investigated in vitro for their anticomplement activity against the classical pathway of the complement system. Of these, compound 1 showed significant anticomplement activity with 50% inhibitory concentration (IC50) value of 56.98 μM, whereas compounds 2–7 were inactive.
Bo-Young Park; Byung-Sun Min; Sei-Ryang Oh; Jung-Hee Kim; Tae-Jin Kim; Dong-Hee Kim; Ki-Hwan Bae; Hyeong-Kyu Lee. Isolation and anticomplement activity of compounds from Dendropanax morbifera. Journal of Ethnopharmacology 2004, 90, 403 -408.
AMA StyleBo-Young Park, Byung-Sun Min, Sei-Ryang Oh, Jung-Hee Kim, Tae-Jin Kim, Dong-Hee Kim, Ki-Hwan Bae, Hyeong-Kyu Lee. Isolation and anticomplement activity of compounds from Dendropanax morbifera. Journal of Ethnopharmacology. 2004; 90 (2-3):403-408.
Chicago/Turabian StyleBo-Young Park; Byung-Sun Min; Sei-Ryang Oh; Jung-Hee Kim; Tae-Jin Kim; Dong-Hee Kim; Ki-Hwan Bae; Hyeong-Kyu Lee. 2004. "Isolation and anticomplement activity of compounds from Dendropanax morbifera." Journal of Ethnopharmacology 90, no. 2-3: 403-408.