This page has only limited features, please log in for full access.
Herein, we propose the light scattering intensity of silver and gold alloy as signal transducer to enhance the sensitivity and dynamic linearity of immunoassay for quantitative detection of Cronobacter muytjensii (C. muytjensii) in powered infant formula (PIF). Silver and gold alloy, namely, silver-coated gold nanocomposite ([email protected]), was obtained by growing silver on the surface of multi-dendritic colloidal gold. The silver growth was triggered by the hydrolysis of urease to urea in the presence of silver nitrate and glucose. Owing to integrating the advantages of light scattering of [email protected] and enzyme-catalyzed mediated signal amplification, the developed light scattering based immunoassay shows high sensitivity for detection of C. muytjensii, with a detection limit of 51 CFU/mL, which is three orders of magnitude lower than that of conventional enzyme-linked immunosorbent assay (ELISA, 6.39 × 104 CFU/mL). In addition, the proposed method also exhibits wide dynamic curve from 3.4 × 102 CFU/mL to 3.4 × 109 CFU/mL for quantitative detection of C. muytjensii, and is significantly superior conventional ELISA (3.4 × 105 CFU/mL to 3.4 × 109 CFU/mL). The accuracy and precision of the proposed immunoassy were evaluated by analysis of C. muytjensii-fortified PIF samples. The average recoveries of the intra- and inter-assays are within 87.90 %–107.91 %, and the coefficient of variation ranges from 2.72 %–9.32 %. This finding indicates an acceptable accuracy for quantitative detection of C. muytjensii in real PIF samples.
Bao Gao; Tongtong Ma; Lin Feng; Xiaolin Huang; Xuelan Chen; Yonghua Xiong. Light scattering intensity as signal transducer to enhance the performance of immunoassay for Cronobacter detection in powdered infant formula. Sensors and Actuators B: Chemical 2021, 344, 130312 .
AMA StyleBao Gao, Tongtong Ma, Lin Feng, Xiaolin Huang, Xuelan Chen, Yonghua Xiong. Light scattering intensity as signal transducer to enhance the performance of immunoassay for Cronobacter detection in powdered infant formula. Sensors and Actuators B: Chemical. 2021; 344 ():130312.
Chicago/Turabian StyleBao Gao; Tongtong Ma; Lin Feng; Xiaolin Huang; Xuelan Chen; Yonghua Xiong. 2021. "Light scattering intensity as signal transducer to enhance the performance of immunoassay for Cronobacter detection in powdered infant formula." Sensors and Actuators B: Chemical 344, no. : 130312.
Tailored to the increasing demands for sensing technologies, the fabrication of dual-modal sensing technologies through combining two signal transduction channels into one method has been proposed and drawn considerable attention. The integration of two sensing signals not only promotes the analytical efficiency with reduced assumption, but also improves the analytical performances with enlarged detection linear range, enhanced accuracy, and boosted application flexibility. The two top-rated output signals for developing dual-modal sensors are colorimetric and fluorescent signals because of their outstanding merits for point of care applications and real-time sensitive sensing. Given the rapid development of material chemistry and nanotechnology, the recent decade has witnessed great advance in colorimetric/fluorimetric signal based dual-modal sensing technologies. The new sensing strategy leads to a broad avenue for various applications in disease diagnosis, environmental monitoring and food safety because of the complementary and synergistic effects of the two output signals. In this state-of-the-art review, we comprehensively summarize different types of colorimetric/fluorimetric dual-modal sensing methods by highlighting representative research in the last 5 years, digging into their sensing methodologies, particularly the working principles of the signal transduction systems. Then, the challenges and future prospects for boosting further development of this research field are discussed.
Yaofeng Zhou; Xiaolin Huang; Xinyu Hu; Weipeng Tong; Yuankui Leng; Yonghua Xiong. Recent advances in colorimetry/fluorimetry-based dual-modal sensing technologies. Biosensors and Bioelectronics 2021, 190, 113386 .
AMA StyleYaofeng Zhou, Xiaolin Huang, Xinyu Hu, Weipeng Tong, Yuankui Leng, Yonghua Xiong. Recent advances in colorimetry/fluorimetry-based dual-modal sensing technologies. Biosensors and Bioelectronics. 2021; 190 ():113386.
Chicago/Turabian StyleYaofeng Zhou; Xiaolin Huang; Xinyu Hu; Weipeng Tong; Yuankui Leng; Yonghua Xiong. 2021. "Recent advances in colorimetry/fluorimetry-based dual-modal sensing technologies." Biosensors and Bioelectronics 190, no. : 113386.
Owing to the over-increasing demands in resisting and managing the coronavirus disease 2019 (COVID-19) pandemic, development of rapid, highly sensitive, accurate, and versatile tools for monitoring total antibody concentrations at the population level has been evolved as an urgent challenge on measuring the fatality rate, tracking the changes in incidence and prevalence, comprehending medical sequelae after recovery, as well as characterizing seroprevalence and vaccine coverage. To this end, herein we prepared highly luminescent quantum dot nanobeads (QBs) by embedding numerous quantum dots into polymer matrix, and then applied it as a signal-amplification label in lateral flow immunoassay (LFIA). After covalently linkage with the expressed recombinant SARS-CoV-2 spike protein (RSSP), the synthesized QBs were used to determine the total antibody levels in sera by virtue of a double-antigen sandwich immunoassay. Under the developed condition, the QB-LFIA can allow the rapid detection of SARS-CoV-2 total antibodies within 15 min with about one order of magnitude improvement in analytical sensitivity compared to conventional gold nanoparticle-based LFIA. In addition, the developed QB-LFIA performed well in clinical study in dynamic monitoring of serum antibody levels in the whole course of SARS-CoV-2 infection. In conclusion, we successfully developed a promising fluorescent immunological sensing tool for characterizing the host immune response to SARS-CoV-2 infection and confirming the acquired immunity to COVID-19 by evaluating the SRAS-CoV-2 total antibody level in the crowd.
Yaofeng Zhou; Yuan Chen; Wenjuan Liu; Hao Fang; Xiangmin Li; Li Hou; Yuanjie Liu; Weihua Lai; Xiaolin Huang; Yonghua Xiong. Development of a rapid and sensitive quantum dot nanobead-based double-antigen sandwich lateral flow immunoassay and its clinical performance for the detection of SARS-CoV-2 total antibodies. Sensors and Actuators B: Chemical 2021, 343, 130139 -130139.
AMA StyleYaofeng Zhou, Yuan Chen, Wenjuan Liu, Hao Fang, Xiangmin Li, Li Hou, Yuanjie Liu, Weihua Lai, Xiaolin Huang, Yonghua Xiong. Development of a rapid and sensitive quantum dot nanobead-based double-antigen sandwich lateral flow immunoassay and its clinical performance for the detection of SARS-CoV-2 total antibodies. Sensors and Actuators B: Chemical. 2021; 343 ():130139-130139.
Chicago/Turabian StyleYaofeng Zhou; Yuan Chen; Wenjuan Liu; Hao Fang; Xiangmin Li; Li Hou; Yuanjie Liu; Weihua Lai; Xiaolin Huang; Yonghua Xiong. 2021. "Development of a rapid and sensitive quantum dot nanobead-based double-antigen sandwich lateral flow immunoassay and its clinical performance for the detection of SARS-CoV-2 total antibodies." Sensors and Actuators B: Chemical 343, no. : 130139-130139.
The analytical performance of immunochromatographic assay (ICA) is usually determined by the biological activity of antibody and gold nanoparticle conjugates (AuNP probes). However, conventional probes are constructed using the nondirectional coupling method that can cause the improper orientation of antibodies with the poor accessibility of antigen-binding sites. To address these issues, we report a site-specific directional coupling strategy to enhance the bioactivity of AuNP probes through the specific covalent binding of the aldehyde group in the Fc domain of antibodies with the hydrazide group modified on the surface of AuNPs. Through this design, the antibodies can be erected on the AuNP surface to fully expose the Fab domain and achieve the maximized functional availability. Leveraging these AuNP probes as ICA labels, we demonstrate an improved detection of the hepatitis B surface antigen with less used amount of labeled antibody (0.2 mg/pmol AuNPs), shorter reaction time (10 min), better antibody bioactivity, and higher detection sensitivity (2 ng/mL) compared with the carbodiimide method. Overall, this work provides great promise for the design and the construction of high-performance probes to enhance the detection performance of ICA sensors.
Shu Zhou; Jing Hu; Xirui Chen; Hong Duan; Yanna Shao; Tong Lin; Xiangmin Li; Xiaolin Huang; Yonghua Xiong. Hydrazide-assisted directional antibody conjugation of gold nanoparticles to enhance immunochromatographic assay. Analytica Chimica Acta 2021, 1168, 338623 .
AMA StyleShu Zhou, Jing Hu, Xirui Chen, Hong Duan, Yanna Shao, Tong Lin, Xiangmin Li, Xiaolin Huang, Yonghua Xiong. Hydrazide-assisted directional antibody conjugation of gold nanoparticles to enhance immunochromatographic assay. Analytica Chimica Acta. 2021; 1168 ():338623.
Chicago/Turabian StyleShu Zhou; Jing Hu; Xirui Chen; Hong Duan; Yanna Shao; Tong Lin; Xiangmin Li; Xiaolin Huang; Yonghua Xiong. 2021. "Hydrazide-assisted directional antibody conjugation of gold nanoparticles to enhance immunochromatographic assay." Analytica Chimica Acta 1168, no. : 338623.
The ultrasensitive detection of disease-related biomarkers is critical to improving the early diagnosis, monitoring, and treatment of diseases. Herein, we reported a dynamic light scattering (DLS)-based immunosensor for the high-sensitivity detection of alpha-fetoprotein (AFP), an important biomarker in the early diagnosis of primary liver cancer. A metal–organic framework and gold nanoparticle composite ([email protected]) was designed as a low-background scattering signal probe. After its structure was decomposed in a weak alkaline solution, the numerous small-sized AuNPs (∼4 nm) were released from the MOF structure. Controllable gold in situ growth strategy without self-nucleation was then executed to increase the AuNP size. Owing to the double signal amplification of the burst release of numerous small-sized AuNPs from MOFs and the gold in situ growth for an enhanced AuNP size, the proposed DLS immunosensor was successfully applied for the ultrasensitive detection of AFP with the detection limit of 0.36 pg mL−1. The reliability of the proposed method was evaluated by analyzing 22 actual clinic serum samples, and the results were further verified by the chemiluminescence immunosensor. This work features [email protected] and the gold in situ growth amplified modulation of DLS-based signal transduction, which demonstrates the great potential as a novel bioanalytical platform for a myriad of purposes.
Yu Li; Jing Yuan; Shengnan Zhan; Jiaqi Hu; Yuqian Guo; Lu Ding; Xiaolin Huang; Yonghua Xiong. Dynamic light scattering immunosensor based on metal-organic framework mediated gold growth strategy for the ultra-sensitive detection of alpha-fetoprotein. Sensors and Actuators B: Chemical 2021, 341, 130030 .
AMA StyleYu Li, Jing Yuan, Shengnan Zhan, Jiaqi Hu, Yuqian Guo, Lu Ding, Xiaolin Huang, Yonghua Xiong. Dynamic light scattering immunosensor based on metal-organic framework mediated gold growth strategy for the ultra-sensitive detection of alpha-fetoprotein. Sensors and Actuators B: Chemical. 2021; 341 ():130030.
Chicago/Turabian StyleYu Li; Jing Yuan; Shengnan Zhan; Jiaqi Hu; Yuqian Guo; Lu Ding; Xiaolin Huang; Yonghua Xiong. 2021. "Dynamic light scattering immunosensor based on metal-organic framework mediated gold growth strategy for the ultra-sensitive detection of alpha-fetoprotein." Sensors and Actuators B: Chemical 341, no. : 130030.
Herein, we report a novel multiplex fluorescent competitive immunosensor using self-luminous bacteria as a bifunctional probe for the simultaneous detection and quantification of multiple pathogens. The self-luminous bacteria are prepared through the facile one-step co-culture of bacteria and organic fluorescent dyes. Cronobacter muytjensii, Listeria monocytogenes, and Escherichia coli O157:H7 are fluorescently labelled with 7-hydroxycoumarin, fluorescein isothiocyanate, and rhodamine B, respectively, due to the dyes small spectral overlap in excitation/emission. The obtained fluorescent bacteria retain specific immunorecognition activity, but exhibit excellent fluorescent sensing ability. By using these fluorescent bacteria as bifunctional probes for competitive recognition and optical sensing, we achieved the sensitive and simultaneous quantitative determination of three pathogens with sensitivities of below 8.6 × 103 CFU/mL. The sensitivities can be significantly enhanced to 1 CFU/mL in real milk by integrating with a pre-incubation step, showing great potential for the ultrasensitive determination of trace bacteria and even single colony with the overall detection time of less than 10 h. In summary, this work provides an alternative multiplexing analytical platform as a powerful and rapid diagnostic tool for monitoring foodborne pathogen contamination.
Yuqian Guo; Yaofeng Zhou; Jinmei Fu; Hao Fang; Yu Li; Xiaolin Huang; Yonghua Xiong. A self-luminous bifunctional bacteria directed fluorescent immunosensor for the simultaneous detection and quantification of three pathogens in milk. Sensors and Actuators B: Chemical 2021, 338, 129757 .
AMA StyleYuqian Guo, Yaofeng Zhou, Jinmei Fu, Hao Fang, Yu Li, Xiaolin Huang, Yonghua Xiong. A self-luminous bifunctional bacteria directed fluorescent immunosensor for the simultaneous detection and quantification of three pathogens in milk. Sensors and Actuators B: Chemical. 2021; 338 ():129757.
Chicago/Turabian StyleYuqian Guo; Yaofeng Zhou; Jinmei Fu; Hao Fang; Yu Li; Xiaolin Huang; Yonghua Xiong. 2021. "A self-luminous bifunctional bacteria directed fluorescent immunosensor for the simultaneous detection and quantification of three pathogens in milk." Sensors and Actuators B: Chemical 338, no. : 129757.
The use of luminescent nanobeads to improve the sensitivity of sandwich immunochromatographic assay (ICA) has obtained increasing concern. Illustrating the relationship among luminescent intensity, nanobead size, nitrocellulose membrane aperture, and ICA sensitivity is important for achieving the optimal target detection. Thus, we synthesized six differently sized quantum dot beads (QBs) (95, 140, 180, 235, 325, and 405 nm) as ICA labels and applied them in three aperture membranes (10, 15, and 25 μm). Results indicate that increasing the QB size to less than an appropriate size of 235 nm is beneficial for ICA sensitivity because of the increased fluorescence. However, oversized QBs result in reduced sensitivity due to the decreased diffusion or settlement of the QB on the membrane that causes obvious background signal. The small aperture membrane perfectly matching the QB size contributes to ICA sensitivity by decreasing the migration velocity of the QB probe for increased binding of the [email protected] complex at the T zone. Consequently, the best detection of hepatitis B surface antigen with a sensitivity of 0.156 ng/mL is achieved using 235 nm QBs in 15 μm membrane. Further performance evaluation of our QB235-ICACN95 demonstrates excellent accuracy, selectivity, and practicability. This work provides a new idea to manipulate the sensitivity of sandwich ICA by tuning the QB size and the membrane aperture, and a theoretical guidance for selecting the probe and membrane to achieve the best detection of target analytes.
Hong Duanab; Xirui Chenab; Yuhao Wuab; Yuankui Lengab; Xiaolin Huangab; Yonghua Xiongabc. Integrated nanoparticle size with membrane porosity for improved analytical performance in sandwich immunochromatographic assay. Analytica Chimica Acta 2020, 1141, 136 -143.
AMA StyleHong Duanab, Xirui Chenab, Yuhao Wuab, Yuankui Lengab, Xiaolin Huangab, Yonghua Xiongabc. Integrated nanoparticle size with membrane porosity for improved analytical performance in sandwich immunochromatographic assay. Analytica Chimica Acta. 2020; 1141 ():136-143.
Chicago/Turabian StyleHong Duanab; Xirui Chenab; Yuhao Wuab; Yuankui Lengab; Xiaolin Huangab; Yonghua Xiongabc. 2020. "Integrated nanoparticle size with membrane porosity for improved analytical performance in sandwich immunochromatographic assay." Analytica Chimica Acta 1141, no. : 136-143.
A polyethyleneimine (PEI)-assisted copper in-situ growth (CISG) strategy was proposed as a controlled signal amplification strategy to enhance the sensitivity of gold nanoparticle-based lateral flow sensors (AuNP-LFS). The controlled signal amplification is achieved by introducing PEI as a structure-directing agent to regulate the thermodynamics of anisotropic Cu nanoshell growth on the AuNP surface, thus controlling shape and size of the resultant [email protected] core–shell nanostructures and confining free reduction and self-nucleation of Cu2+ for improved reproducibility and decreased false positives. The PEI-CISG-enhanced AuNP-LFS showed ultrahigh sensitivities with the detection limits of 50 fg mL−1 for HIV-1 capsid p24 antigen and 6 CFU mL−1 for Escherichia coli O157:H7. We further demonstrated its clinical diagnostic efficacy by configuring PEI-CISG into a commercial AuNP-LFS detection kit for SARS-CoV-2 antibody detection. Altogether, this work provides a reliable signal amplification platform to dramatically enhance the sensitivity of AuNP-LFS for rapid and accurate diagnostics of various infectious diseases.
Yaofeng Zhou; Yuan Chen; Yang Liu; Hao Fang; Xiaolin Huang; Yuankui Leng; Zhengqiong Liu; Li Hou; Wei Zhang; Weihua Lai; Yonghua Xiong. Controlled copper in situ growth-amplified lateral flow sensors for sensitive, reliable, and field-deployable infectious disease diagnostics. Biosensors and Bioelectronics 2020, 171, 112753 -112753.
AMA StyleYaofeng Zhou, Yuan Chen, Yang Liu, Hao Fang, Xiaolin Huang, Yuankui Leng, Zhengqiong Liu, Li Hou, Wei Zhang, Weihua Lai, Yonghua Xiong. Controlled copper in situ growth-amplified lateral flow sensors for sensitive, reliable, and field-deployable infectious disease diagnostics. Biosensors and Bioelectronics. 2020; 171 ():112753-112753.
Chicago/Turabian StyleYaofeng Zhou; Yuan Chen; Yang Liu; Hao Fang; Xiaolin Huang; Yuankui Leng; Zhengqiong Liu; Li Hou; Wei Zhang; Weihua Lai; Yonghua Xiong. 2020. "Controlled copper in situ growth-amplified lateral flow sensors for sensitive, reliable, and field-deployable infectious disease diagnostics." Biosensors and Bioelectronics 171, no. : 112753-112753.
Compared with absorbance, scattering-based dynamic light scattering (DLS) signal has higher sensitivity because its light-scattering intensity is very sensitive to changes in size, thereby enhancing the sensitivity. Herein, we first developed a DLS-enhanced direct competitive enzyme-linked immunosorbent assay (DLS-dcELISA) for ultrasensitive detection of aflatoxin B1 (AFB1) in corn. By using hydroxyl radical-induced gold nanoparticle (AuNP) aggregation to amplify AuNP scattering signals, the developed DLS-dcELISA exhibited ultrahigh sensitivity for AFB1. The detection limit was 0.12 pg mL−1, which was 153- and 385-fold lower than those obtained using plasmonic and colorimetric dcELISA. In addition, the DLS-dcELISA exhibited excellent selectivity, high accuracy, and strong practicality. Overall, this work presented a simple and universal strategy for improving the sensitivity of traditional ELISA platform only by using the sensitive DLS signals. This technique can replace absorbance-based plasmonic or colored signals as immunoassay signal output for enhanced competitive detection of mycotoxins.
Shengnan Zhan; Jiaqi Hu; Yu Li; Xiaolin Huang; Yonghua Xiong. Direct competitive ELISA enhanced by dynamic light scattering for the ultrasensitive detection of aflatoxin B1 in corn samples. Food Chemistry 2020, 342, 128327 .
AMA StyleShengnan Zhan, Jiaqi Hu, Yu Li, Xiaolin Huang, Yonghua Xiong. Direct competitive ELISA enhanced by dynamic light scattering for the ultrasensitive detection of aflatoxin B1 in corn samples. Food Chemistry. 2020; 342 ():128327.
Chicago/Turabian StyleShengnan Zhan; Jiaqi Hu; Yu Li; Xiaolin Huang; Yonghua Xiong. 2020. "Direct competitive ELISA enhanced by dynamic light scattering for the ultrasensitive detection of aflatoxin B1 in corn samples." Food Chemistry 342, no. : 128327.
Immunochromatographic assays (ICAs) are one of the most popular on-site diagnostic tools. The bio-activity of the probe holds great promise for the detection performance of ICA. The orientation of antibodies on nanoparticle surface plays a vital role in improving the bio-activity of the probe. Hydrazide mediated oriented coupling (HDZ-O) strategy can erect the antibodies on the particle surface via the nucleophilic addition reaction between the hydrazide group of particles and the aldehyde group of the Fc region of antibodies. In this work, we synthesized the hydrazide and carboxylic group modified quantum dot beads (QBs-NH-NH2, and QBs-COOH) and compared two coupling strategies for preparing QB probes systematically. Results showed the reaction time for producing QB probe by using HDZ-O method was 20 min, and the optimal labeled antibody content was 80 μg per mg of QBs. Those for carbodiimide method were 180 min and 320 μg per mg of QBs. Moreover, the QB probe by HDZ-O showed a higher bio-activity than that by carbodiimide method under their optimal labeled antibody concentrations. Subsequently, the QB probe by HDZ-O strategy was applied further in sandwich ICA for hepatitis B surface antigen (HBsAg) detection. The results indicated that the strip has a widely dynamic linearity with HBsAg concentration ranging from 0.05 ng/mL to 3200 ng/mL. The limit of detection of the strip is 0.05 ng/mL, which is approximately fourfold lower than that of the probe prepared by carbodiimide method. In addition, the ICA strip also showed acceptable accuracy and precision for HBsAg quantitative detection in real serum samples, which have average recoveries in intra- and inter-assays that vary from 89.16%–97.63% with the variable coefficients less than 6.98%. The proposed ICA strip showed good agreement (R2 > 0.94) with a commercial chemiluminescence kit for 40 of clinical serum samples. All in all, this study proposed a robust strategy to immobilize the antibodies with proper orientation on the surface of QBs, and the resultant QB probe can improve the detection performance of sandwich LFIA platform remarkably.
Jing Hu; Shu Zhou; Lifeng Zeng; Qi Chen; Hong Duan; Xirui Chen; Xiangmin Li; Yonghua Xiong. Hydrazide mediated oriented coupling of antibodies on quantum dot beads for enhancing detection performance of immunochromatographic assay. Talanta 2020, 223, 121723 .
AMA StyleJing Hu, Shu Zhou, Lifeng Zeng, Qi Chen, Hong Duan, Xirui Chen, Xiangmin Li, Yonghua Xiong. Hydrazide mediated oriented coupling of antibodies on quantum dot beads for enhancing detection performance of immunochromatographic assay. Talanta. 2020; 223 ():121723.
Chicago/Turabian StyleJing Hu; Shu Zhou; Lifeng Zeng; Qi Chen; Hong Duan; Xirui Chen; Xiangmin Li; Yonghua Xiong. 2020. "Hydrazide mediated oriented coupling of antibodies on quantum dot beads for enhancing detection performance of immunochromatographic assay." Talanta 223, no. : 121723.
Escherichia coli O157:H7 is a common harmful foodborne pathogen that can cause severe diseases at low infectious doses. Traditional lateral flow immunoassay (LFIA) for the rapid screening of E. coli O157:H7 in food suffers from low sensitivity due to its dependence on 20- to 40-nm gold nanoparticles (AuNP) with insufficient brightness as labels. To address this issue, we reported for the first time the successful synthesis of gold superparticles (GSP) by encapsulating numerous small AuNP into a polymer nanobead using an evaporation-induced self-assembly method. Results indicated that the resultant GSP exhibited remarkably enhanced absorbance compared with the most widely used 40 nm AuNP in LFIA. In addition, the absorbance of GSP could be easily tuned by varying GSP sizes. Under optimized conditions, we achieved a rapid and sensitive determination of E. coli O157:H7 in milk with a detection limit of 5.95 × 102 cfu/mL when using the GSP with a size of 342 nm as LFIA signal reporters, exhibiting improvement of approximately 32-fold relative to the conventional 40 nm AuNP-LFIA method. We further demonstrated the selectivity, accuracy, reliability, and practicality of the proposed GSP-LFIA strip. In summary, this work offers a new strategy for improving LFIA sensitivity using assembled GSP as markers and demonstrates huge potential in rapidly and sensitively detecting foodborne pathogens.
Yu Li; Xirui Chen; Jing Yuan; Yuankui Leng; Weihua Lai; Xiaolin Huang; Yonghua Xiong. Integrated gold superparticles into lateral flow immunoassays for the rapid and sensitive detection of Escherichia coli O157:H7 in milk. Journal of Dairy Science 2020, 103, 6940 -6949.
AMA StyleYu Li, Xirui Chen, Jing Yuan, Yuankui Leng, Weihua Lai, Xiaolin Huang, Yonghua Xiong. Integrated gold superparticles into lateral flow immunoassays for the rapid and sensitive detection of Escherichia coli O157:H7 in milk. Journal of Dairy Science. 2020; 103 (8):6940-6949.
Chicago/Turabian StyleYu Li; Xirui Chen; Jing Yuan; Yuankui Leng; Weihua Lai; Xiaolin Huang; Yonghua Xiong. 2020. "Integrated gold superparticles into lateral flow immunoassays for the rapid and sensitive detection of Escherichia coli O157:H7 in milk." Journal of Dairy Science 103, no. 8: 6940-6949.
Conventional gold nanoparticle-based lateral flow immunoassay (LFIA) usually suffers a huge challenge in measuring target concentration in food matrices with dark color because of its poor resistance to the background matrix and color interference. To address this issue, we first report a novel bifunctional magneto-gold nanohybrid (MGNH) for the simultaneous magnetic separation and colorimetric target sensing by integrating MGNHs into LFIA. Under optimum conditions, an ultrasensitive detection of ochratoxin A (OTA) in grape juice was achieved with a limit of detection at 0.094 ng mL−1. The average recoveries of this MGNH-LFIA ranged from 92.31% to 108.97% with a coefficient of variation of below 12%. The excellent selectivity of our MGNH–LFIA against OTA was demonstrated. Besides, our MGNH–LFIA is comparable to liquid chromatography coupled with mass spectrometry in terms of accuracy, reproducibility, and practicability. The designed MGNH-LFIA platform is readily extended for improving other small molecule detection in food samples.
Liangwen Hao; Jing Chen; Xirui Chen; Tongtong Ma; Xiaoxia Cai; Hong Duan; Yuankui Leng; Xiaolin Huang; Yonghua Xiong. A novel magneto-gold nanohybrid-enhanced lateral flow immunoassay for ultrasensitive and rapid detection of ochratoxin A in grape juice. Food Chemistry 2020, 336, 127710 .
AMA StyleLiangwen Hao, Jing Chen, Xirui Chen, Tongtong Ma, Xiaoxia Cai, Hong Duan, Yuankui Leng, Xiaolin Huang, Yonghua Xiong. A novel magneto-gold nanohybrid-enhanced lateral flow immunoassay for ultrasensitive and rapid detection of ochratoxin A in grape juice. Food Chemistry. 2020; 336 ():127710.
Chicago/Turabian StyleLiangwen Hao; Jing Chen; Xirui Chen; Tongtong Ma; Xiaoxia Cai; Hong Duan; Yuankui Leng; Xiaolin Huang; Yonghua Xiong. 2020. "A novel magneto-gold nanohybrid-enhanced lateral flow immunoassay for ultrasensitive and rapid detection of ochratoxin A in grape juice." Food Chemistry 336, no. : 127710.
As a global public health problem, food safety has attracted increasing concern. To minimize the risk exposure of food to harmful ingredients, food quality and safety inspection that covers the whole process of “from farm to fork” is much desired. Fluorescent sensing is a promising and powerful screening tool for sensing hazardous substances in food and thus plays a crucial role in promoting food safety assurance. However, traditional fluorphores generally suffer the problem of aggregation‐caused quenching (ACQ) effect, which limit their application in food quality and safety inspection. In this regard, luminogens with aggregation‐induced emission property (AIEgens) showed large potential in food analysis since AIEgens effectively surmount the ACQ effect with much better detection sensitivity, accuracy, and robustness. In this contribution, we review the latest developments of food safety monitoring by AIEgens, which will focus on the molecular design of AIEgens and their sensing principles. Several examples of AIE‐based sensing applications for screening food contaminations are highlighted, and future perspectives and challenges in this emerging field are tentatively elaborated. We hope this review can motivate new research ideas and interest to aid food safety and quality control, and facilitate more collaborative endeavors to advance the state‐of‐the‐art sensing developments and reduce actual translational gap between laboratory research and industrial production.
Xiaolin Huang; Qian Guo; Ruoyao Zhang; Zheng Zhao; Yuankui Leng; Jacky W. Y. Lam; Yonghua Xiong; Ben Zhong Tang. AIEgens: An emerging fluorescent sensing tool to aid food safety and quality control. Comprehensive Reviews in Food Science and Food Safety 2020, 19, 2297 -2329.
AMA StyleXiaolin Huang, Qian Guo, Ruoyao Zhang, Zheng Zhao, Yuankui Leng, Jacky W. Y. Lam, Yonghua Xiong, Ben Zhong Tang. AIEgens: An emerging fluorescent sensing tool to aid food safety and quality control. Comprehensive Reviews in Food Science and Food Safety. 2020; 19 (4):2297-2329.
Chicago/Turabian StyleXiaolin Huang; Qian Guo; Ruoyao Zhang; Zheng Zhao; Yuankui Leng; Jacky W. Y. Lam; Yonghua Xiong; Ben Zhong Tang. 2020. "AIEgens: An emerging fluorescent sensing tool to aid food safety and quality control." Comprehensive Reviews in Food Science and Food Safety 19, no. 4: 2297-2329.
In this study, we developed an indirect competitive plasmonic immunoassay using glucose oxidase (GOx)-induced redox reaction to etch Au nanorods (AuNRs) for qualitative and quantitative detection of aflatoxin M1 (AFM1) in milk. In this system, streptavidin (SA) was selected as a linker between biotinylated anti-AFM1-monoantibody (bio-mAb) and biotinylated GOx (bio-GOx) to form the immunocomplexes bio-mAb-SA-bio-GOx. After the oxidation of the glucose and I-, the resultant I2 could etch cetytrimethylammonium bromide (CTAB)-stabilized AuNRs. This resulted in the blue shift of the longitudinal localized surface plasmon resonance (LSPR) extinction peak, with a color change from blue to pink. The linear range and limit of detection (LOD) of the plasmonic immunoassay were 0.25-10 ng mL-1 and 0.11 ng mL-1, respectively. It displayed quantitative detection with high sensitivity, specificity, recovery, and accuracy, which is promising for qualitative and quantitative detection of AFM1 in food safety.
Bolong Fang; Shaolan Xu; Youju Huang; Fengmei Su; Zhen Huang; Hao Fang; Juan Peng; Yonghua Xiong; Weihua Lai. Gold nanorods etching-based plasmonic immunoassay for qualitative and quantitative detection of aflatoxin M1 in milk. Food Chemistry 2020, 329, 127160 .
AMA StyleBolong Fang, Shaolan Xu, Youju Huang, Fengmei Su, Zhen Huang, Hao Fang, Juan Peng, Yonghua Xiong, Weihua Lai. Gold nanorods etching-based plasmonic immunoassay for qualitative and quantitative detection of aflatoxin M1 in milk. Food Chemistry. 2020; 329 ():127160.
Chicago/Turabian StyleBolong Fang; Shaolan Xu; Youju Huang; Fengmei Su; Zhen Huang; Hao Fang; Juan Peng; Yonghua Xiong; Weihua Lai. 2020. "Gold nanorods etching-based plasmonic immunoassay for qualitative and quantitative detection of aflatoxin M1 in milk." Food Chemistry 329, no. : 127160.
An ultra-high sensitivity label-free optical fiber biosensor for inactivated Staphylococcus aureus (S. aureus) detection is proposed and investigated in this study, with additional advantages of robust and stability compared to traditional tapered fiber structure. The proposed fiber biosensor is based on a tapered singlemode- no core-singlemode fiber coupler (SNSFC) structure, where the no core fiber was tapered to small diameter (taper-waist diameter of about 10 μm) and functionalized with the pig immunoglobulin G (IgG) antibody for detection of S. aureus. The measured maximum wavelength shift of the sensor for an S. aureus concentration of 7 × 101 CFU/mL (colony forming unit per milliliter) is 2.04 nm, which is equivalent to a limit of detection (LOD) of 3.1 CFU/mL (a highest LOD reported so far for optical fiber biosensors), considering the maximum wavelength variation of the sensor in phosphate buffered saline (PBS) is ±0.03 nm over 40 min, where 3 times of maximum wavelength variation (3 × 0.03 = 0.09 nm) is defined as measurement limit. The response time of the developed fiber sensor is less than 30 min. The ultra-sensitive biosensor has potential to be widely applied to various areas such as disease, medical diagnostics and food safety inspection.
Ling Chen; Yuan-Kui Leng; Bin Liu; Juan Liu; Sheng-Peng Wan; Tao Wu; Jinhui Yuan; Liyang Shao; Guoqiang Gu; Yong Qing Fu; Hengyi Xu; Yonghua Xiong; Xing-Dao He; Qiang Wu. Ultrahigh-sensitivity label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection. Sensors and Actuators B: Chemical 2020, 320, 128283 .
AMA StyleLing Chen, Yuan-Kui Leng, Bin Liu, Juan Liu, Sheng-Peng Wan, Tao Wu, Jinhui Yuan, Liyang Shao, Guoqiang Gu, Yong Qing Fu, Hengyi Xu, Yonghua Xiong, Xing-Dao He, Qiang Wu. Ultrahigh-sensitivity label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection. Sensors and Actuators B: Chemical. 2020; 320 ():128283.
Chicago/Turabian StyleLing Chen; Yuan-Kui Leng; Bin Liu; Juan Liu; Sheng-Peng Wan; Tao Wu; Jinhui Yuan; Liyang Shao; Guoqiang Gu; Yong Qing Fu; Hengyi Xu; Yonghua Xiong; Xing-Dao He; Qiang Wu. 2020. "Ultrahigh-sensitivity label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection." Sensors and Actuators B: Chemical 320, no. : 128283.
Herein, we report a multicolor immunochromatographic test strip (ICTS) nanosensor for the rapid and simultaneous visual detection of fumonisin B1 (FB1), zearalenone (ZEN), ochratoxin A (OTA), and aflatoxin B1 (AFB1). Four colored AuNPs, including gold nanospheres (red), gold nanocacti (purple), gold nanoflowers (blue), and hyperbranched Au plasmonic blackbodies (black), were synthesized and functionalized with the corresponding antibodies to target each analyte. The developed multicolor platform improved bare-eye-based visual detection ability with cut-off values of 120, 60, 2.5, and 5 ng/mL for FB1, ZEN, OTA, and AFB1, respectively. The limits of detection for FB1, ZEN, OTA, and AFB1 were calculated as 3.27, 0.70, 0.10, and 0.06 ng/mL, respectively, and the average recoveries of the four mycotoxins ranged from 82.36% to 116.23% with a small coefficient of variation (below 12.98%). Accuracy, stability, reliability, and practicability were demonstrated by analyzing artificially and naturally contaminated corn samples. In summary, the designed multicolor ICTS nanosensor can serve as an alternative rapid diagnostic platform enabling the sensitive, simultaneous, and on-site screening detection of mycotoxins and other analytes.
Yuhao Wu; Yaofeng Zhou; Hai Huang; Xirui Chen; Yuankui Leng; Weihua Lai; Xiaolin Huang; Yonghua Xiong. Engineered gold nanoparticles as multicolor labels for simultaneous multi-mycotoxin detection on the immunochromatographic test strip nanosensor. Sensors and Actuators B: Chemical 2020, 316, 128107 .
AMA StyleYuhao Wu, Yaofeng Zhou, Hai Huang, Xirui Chen, Yuankui Leng, Weihua Lai, Xiaolin Huang, Yonghua Xiong. Engineered gold nanoparticles as multicolor labels for simultaneous multi-mycotoxin detection on the immunochromatographic test strip nanosensor. Sensors and Actuators B: Chemical. 2020; 316 ():128107.
Chicago/Turabian StyleYuhao Wu; Yaofeng Zhou; Hai Huang; Xirui Chen; Yuankui Leng; Weihua Lai; Xiaolin Huang; Yonghua Xiong. 2020. "Engineered gold nanoparticles as multicolor labels for simultaneous multi-mycotoxin detection on the immunochromatographic test strip nanosensor." Sensors and Actuators B: Chemical 316, no. : 128107.
Conventional lateral flow test strip (LFTS) sensors are insufficiently accurate and reliable due to their single-target detection with limited sample information in a single test. The increasing demand for the simultaneous determination of multiple analytes has recently been accelerating the rapid development of high-throughput and multiplexed LFTS sensing technologies. In this contribution, we systematically summarize the recent achievements on the design, development, and application of multiplexed LFTS sensors for improved rapid on-site diagnostics. The discussion focuses on emerging design strategies to increase multiplexing capacity for enhancing analytical efficiency and precision. As a proof-of-concept, several typical examples are presented. The advantages and disadvantages of such approaches are critically analyzed. Finally, we briefly discuss the current challenges and future perspectives.
Yuhao Wu; Yaofeng Zhou; Yuankui Leng; Weihua Lai; Xiaolin Huang; Yonghua Xiong. Emerging design strategies for constructing multiplex lateral flow test strip sensors. Biosensors and Bioelectronics 2020, 157, 112168 .
AMA StyleYuhao Wu, Yaofeng Zhou, Yuankui Leng, Weihua Lai, Xiaolin Huang, Yonghua Xiong. Emerging design strategies for constructing multiplex lateral flow test strip sensors. Biosensors and Bioelectronics. 2020; 157 ():112168.
Chicago/Turabian StyleYuhao Wu; Yaofeng Zhou; Yuankui Leng; Weihua Lai; Xiaolin Huang; Yonghua Xiong. 2020. "Emerging design strategies for constructing multiplex lateral flow test strip sensors." Biosensors and Bioelectronics 157, no. : 112168.
Conventional colloidal gold immunochromatographic assay (AuNP-ICA) cannot meet the requirements for rapid and sensitive detection of Escherichia coli (E. coli) O157:H7 because of its poor sensitivity. Herein, a novel two-step cascade signal amplification strategy that integrates in-situ gold growth and nanozyme-mediated catalytic deposition was proposed to enhance the detection sensitivity of conventional AuNP-ICA dramatically. The enhanced strip displayed ultrahigh sensitivity in E. coli O157:H7 detection and had a detection limit of 1.25×101 CFU/mL, which is approximately 400-fold lower than that of traditional AuNP-ICA (5×103 CFU/mL). The amplified strip had no background signal in the T line zone in the absence of E. coli O157:H7 even after one round of cascade signal amplification. The enhanced strip demonstrated excellent selectivity against E. coli O157:H7 with a negligible cross-reaction to nine other common pathogens. Intra- and inter-assays showed that the improved strip has acceptable accuracy and precision for determining E. coli O157:H7. The average recoveries in a real milk sample ranged from 87.33% to 112.15%, and the coefficients of variation were less than 10%. The enhanced strip also showed great potential in detecting a single E. coli O157:H7 cell in a 75 μL solution.
Jinmei Fu; Yaofeng Zhou; Xiaolin Huang; Wenjing Zhang; Yuhao Wu; Hao Fang; Cunzheng Zhang; Yonghua Xiong. Dramatically Enhanced Immunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coli O157:H7 in Milk. Journal of Agricultural and Food Chemistry 2020, 68, 1118 -1125.
AMA StyleJinmei Fu, Yaofeng Zhou, Xiaolin Huang, Wenjing Zhang, Yuhao Wu, Hao Fang, Cunzheng Zhang, Yonghua Xiong. Dramatically Enhanced Immunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coli O157:H7 in Milk. Journal of Agricultural and Food Chemistry. 2020; 68 (4):1118-1125.
Chicago/Turabian StyleJinmei Fu; Yaofeng Zhou; Xiaolin Huang; Wenjing Zhang; Yuhao Wu; Hao Fang; Cunzheng Zhang; Yonghua Xiong. 2020. "Dramatically Enhanced Immunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coli O157:H7 in Milk." Journal of Agricultural and Food Chemistry 68, no. 4: 1118-1125.
Beyond its extraordinary genome editing ability, the CRISPR-Cas systems have opened a new era of biosensing applications due to its high base resolution and isothermal signal amplification. However, the reported CRISPR-Cas sensors are largely only used for the detection of nucleic acids with limited application for non-nucleic-acid targets. To realize the full potential of the CRISPR-Cas sensors and broaden their applications for detection and quantitation of non-nucleic-acid targets, we herein report CRISPR-Cas12a sensors that are regulated by functional DNA (fDNA) molecules such as aptamers and DNAzymes that are selective for small organic molecule and metal ion detection. The sensors are based on the Cas12a-dependent reporter system consisting of Cas12a, CRISPR RNA (crRNA), and its single-stranded DNA substrate labeled with a fluorophore and quencher at each end (ssDNA-FQ), and fDNA molecules that can lock a DNA activator for Cas12a-crRNA, preventing the ssDNA cleavage function of Cas12a in the absence of the fDNA targets. The presence of fDNA targets can trigger the unlocking of the DNA activator, which can then activate the cleavage of ssDNA-FQ by Cas12a, resulting in an increase of the fluorescent signal detectable by commercially available portable fluorimeters. Using this method, ATP and Na+ have been detected quantitatively under ambient temperature (25 °C) using a simple and fast detection workflow (two steps and <15 min), making the fDNA-regulated CRISPR system suitable for field tests or point-of-care diagnostics. Since fDNAs can be obtained to recognize a wide range of targets, the methods demonstrated here can expand this powerful CRISPR-Cas sensor system significantly to many other targets and thus provide a new toolbox to significantly expand the CRISPR-Cas system into many areas of bioanalytical and biomedical applications.
Ying Xiong; Jingjing Zhang; Zhenglin Yang; Quanbing Mou; Yuan Ma; Yonghua Xiong; Yi Lu. Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets. Journal of the American Chemical Society 2019, 142, 207 -213.
AMA StyleYing Xiong, Jingjing Zhang, Zhenglin Yang, Quanbing Mou, Yuan Ma, Yonghua Xiong, Yi Lu. Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets. Journal of the American Chemical Society. 2019; 142 (1):207-213.
Chicago/Turabian StyleYing Xiong; Jingjing Zhang; Zhenglin Yang; Quanbing Mou; Yuan Ma; Yonghua Xiong; Yi Lu. 2019. "Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets." Journal of the American Chemical Society 142, no. 1: 207-213.
The traditional gold nanoparticle (AuNP) growth-based plasmonic ELISA (pELISA) strictly and directly controlled by reducing reagents can achieve high sensitivity, but it remains fragile toward the surrounding environment. This work developed a sandwich pELISA for Cronobacter detection in powdered infant formula samples by mediating AuNP growth through DNA. In this assay, DNA adsorbed on the surface of gold nanoseeds guided the anisotropic crystal growth with hydroxylamine as a reducing reagent, and the catalase-hydrogen peroxide (Cat-H2O2) system was introduced to bridge the DNA-directed AuNP growth and pELISA, as such DNA can be cleaved into fragments by the hydroxyl radical generated from oxidation of H2O2 through Fenton reagents. Under optimized conditions, the proposed pELISA can qualitatively detect Cronobacter species (Cronobacter muytjensii ATCC 51329) by the naked eye with a cut-off limit of 3 × 105 cfu/mL. This method also revealed a good linear range (3 × 102 to 3 × 107 cfu/mL) for quantitative detection of C. muytjensii ATCC 51329 with a limit of detection of 1.6 × 102 cfu/mL, which is approximately 162.5 times lower than that of horseradish peroxidase-based conventional ELISA (2.6 × 104 cfu/mL). By taking advantage of highly stable DNA-directed AuNP growth, the proposed method shows a good performance in powdered infant formula samples spiked with different concentrations of C. muytjensii ATCC 51329 with average recoveries ranging from 90.79 to 119.09% and coefficient of variation ranging from 4.24 to 9.55%. These values corresponded to an acceptable accuracy and precision for the proposed method. In brief, this work shows potential for screening other analytes in food safety, clinical diagnostics, and environmental monitoring.
Yunqing Wu; Ying Xiong; Xuelan Chen; Dan Luo; Bao Gao; Jing Chen; Xiaolin Huang; Yuankui Leng; Yonghua Xiong. Plasmonic ELISA based on DNA-directed gold nanoparticle growth for Cronobacter detection in powdered infant formula samples. Journal of Dairy Science 2019, 102, 10877 -10886.
AMA StyleYunqing Wu, Ying Xiong, Xuelan Chen, Dan Luo, Bao Gao, Jing Chen, Xiaolin Huang, Yuankui Leng, Yonghua Xiong. Plasmonic ELISA based on DNA-directed gold nanoparticle growth for Cronobacter detection in powdered infant formula samples. Journal of Dairy Science. 2019; 102 (12):10877-10886.
Chicago/Turabian StyleYunqing Wu; Ying Xiong; Xuelan Chen; Dan Luo; Bao Gao; Jing Chen; Xiaolin Huang; Yuankui Leng; Yonghua Xiong. 2019. "Plasmonic ELISA based on DNA-directed gold nanoparticle growth for Cronobacter detection in powdered infant formula samples." Journal of Dairy Science 102, no. 12: 10877-10886.