Biotrickling filters’ control for H₂S removal has special challenges because of complexity of the systems. Feedback and feedforward control were implemented in an anoxic biotrickling filter, operated in co-current flow mode and using nitrite as an electron acceptor. The feedback controller was tuned by three methods—two based on Ziegler-Nichols’ rules (step-response and maintained oscillation) and the third using the Approximate M-constrained Integral Gain Optimization (AMIGO). Inlet H₂S staircase step perturbations were studied using a feedforward control and the effect of EBRT considered by feedback control. The tuning method by maintained oscillation shows the lower errors. The selected controller was a PI, because unstable behavior at the lowest H₂S inlet loading was found under a PID controller. The PI control was able to maintain an outlet H₂S concentration of 14.7 ± 0.45 ppm_V at three EBRT, studied at 117 s, 92 s and 67 s. Therefore, desulfurized biogas could be used to feed a fuel cell. Feedforward control enhances BTF performance compared to the system without control. The maximum outlet H₂S concentration was reduced by 26.18%, although sulfur selectivity did not exceed 55%, as elemental sulfur was the main oxidation product.