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Recently, the demand for natural antioxidants in food manufacturing has increased significantly because of the possible toxicity of synthetic antioxidants. In this context, this research work was performed to evaluate the potentiality of seed (seed with kernel) and pulp (edible portion: pulp with peel) of Syzygium claviflorum to serve as natural antioxidants. The free radical scavenging capacity of S. claviflorum extracts was evaluated by 2,2′-azino-bis (3-ethylbenzothiazolin)-6-sulfonic acid (ABTS), and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assays. In DPPH and ABTS free radical scavenging assays seed extract demonstrated significantly (p < 0.05) higher free radical scavenging and antioxidant properties compared to pulp extract. Free radical-scavenging capacity of S. claviflorum extract from seed was comparable with natural and artificial antioxidants like ascorbic acid and butylated hydroxytoluene (BHT). In ABTS assays, seed extract of S. claviflorum (IC50 = 3.80 ± 0.02 μg/ml) displayed higher free radical-scavenging capacity than the ascorbic acid (IC50 = 10.23 ± 0.06 μg/ml) and BHT with IC50 = 10.23 ± 0.06 μg/ml (a synthetic antioxidant widely used as an additive in food products). In bioactive compound analysis, seed extract was found to have higher amount of total tannin (302.2 ± 4.18 mg TAE g−1 of dry extract), phenolics (206.61 ± 3.78 mg GAE g−1 of dry extract), and flavonoids (1.37 ± 0.23 mg QE g−1 of dry Extract) compared to pulp extract. In addition, nine phenolic compounds were identified in the seed and pulp of S. claviflorum by HPLC-DAD. Among the identified phenolic compounds, the contents of epicatechin, catechin hydrate, and gallic acid were the most dominant phenolic compounds in the seed and pulp extracts. Bioactive and phenolics compounds were highly correlated to antioxidant capacity as measure by the DPPH, ABTS assays. Therefore, it can be summarized that the seed of S. claviflorum is a rich source of phenolic compounds with excellent free radical-scavenging and antioxidant properties and has immense potential to replace the synthetic antioxidants in food preservation.
Shafi Ahmed; Abdullah Jubair; Mohammad Afzal Hossain; Monir Hossain; Shofiul Azam; Mrityunjoy Biswas. Free radical-scavenging capacity and HPLC-DAD screening of phenolic compounds from pulp and seed of Syzygium claviflorum fruit. Journal of Agriculture and Food Research 2021, 6, 100203 .
AMA StyleShafi Ahmed, Abdullah Jubair, Mohammad Afzal Hossain, Monir Hossain, Shofiul Azam, Mrityunjoy Biswas. Free radical-scavenging capacity and HPLC-DAD screening of phenolic compounds from pulp and seed of Syzygium claviflorum fruit. Journal of Agriculture and Food Research. 2021; 6 ():100203.
Chicago/Turabian StyleShafi Ahmed; Abdullah Jubair; Mohammad Afzal Hossain; Monir Hossain; Shofiul Azam; Mrityunjoy Biswas. 2021. "Free radical-scavenging capacity and HPLC-DAD screening of phenolic compounds from pulp and seed of Syzygium claviflorum fruit." Journal of Agriculture and Food Research 6, no. : 100203.
Nontoxic polymers PVP and Na-alginate may provide a favorable environment for the survival of bacteria. Therefore, PVP and Na-alginate were added to a growth medium to develop a liquid inoculant of Bradyrhizobium japonicum strain. The strain was identified by 16S rDNA sequencing. The addition of PVP (1.8%) and Na-alginate (0.2%) in the medium promoted a better survival (1.93 × 109 cells mL−1) of B. japonicum strain compared to the control (3.50 × 102 cells mL−1) after 6 months of storage. The combination of PVP and Na-alginate ensured 1.53 × 107 cells mL−1 up to 12 months of storage under ambient temperature (28 ± 2 °C), whereas PVP (1.8%) or Na-alginate (0.2%) alone produced similar cell counts only up to 8 months and 6 months, respectively. Consecutive field experiments proved the efficacy of the liquid inoculant on nodulation and yield of soybean. The combination of PVP and Na-alginate-based inoculation of B. japonicum strain significantly increased the nodule number per plant, number of pods per plant, number of seeds per pod, seed yield, and yield per hectare (p ≤ 0.05). Thus, the combination of PVP- and Na-alginate-based inoculation of B. japonicum has great potential to popularize the organic cultivation of soybean.
Pulak Maitra; Jubair Al-Rashid; Dipa Mandal; Shofiul Azam; Noorain Rasul. Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of Bradyrhizobium japonicum for Soybean (Glycine max (L.) Merr.). Agronomy 2021, 11, 1009 .
AMA StylePulak Maitra, Jubair Al-Rashid, Dipa Mandal, Shofiul Azam, Noorain Rasul. Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of Bradyrhizobium japonicum for Soybean (Glycine max (L.) Merr.). Agronomy. 2021; 11 (5):1009.
Chicago/Turabian StylePulak Maitra; Jubair Al-Rashid; Dipa Mandal; Shofiul Azam; Noorain Rasul. 2021. "Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of Bradyrhizobium japonicum for Soybean (Glycine max (L.) Merr.)." Agronomy 11, no. 5: 1009.
Mycotoxins are secondary metabolites of filamentous fungi that contaminate food products such as fruits, vegetables, cereals, beverages, and other agricultural commodities. Their occurrence in the food chain, especially in beverages, can pose a serious risk to human health, due to their toxicity, even at low concentrations. Mycotoxins, such as aflatoxins (AFs), ochratoxin A (OTA), patulin (PAT), fumonisins (FBs), trichothecenes (TCs), zearalenone (ZEN), and the alternaria toxins including alternariol, altenuene, and alternariol methyl ether have largely been identified in fruits and their derived products, such as beverages and drinks. The presence of mycotoxins in beverages is of high concern in some cases due to their levels being higher than the limits set by regulations. This review aims to summarize the toxicity of the major mycotoxins that occur in beverages, the methods available for their detection and quantification, and the strategies for their control. In addition, some novel techniques for controlling mycotoxins in the postharvest stage are highlighted.
Shofiul Azam; Shafi Ahmed; Islam; Pulak Maitra; Islam; Dianzhen Yu. Critical Assessment of Mycotoxins in Beverages and Their Control Measures. Toxins 2021, 13, 323 .
AMA StyleShofiul Azam, Shafi Ahmed, Islam, Pulak Maitra, Islam, Dianzhen Yu. Critical Assessment of Mycotoxins in Beverages and Their Control Measures. Toxins. 2021; 13 (5):323.
Chicago/Turabian StyleShofiul Azam; Shafi Ahmed; Islam; Pulak Maitra; Islam; Dianzhen Yu. 2021. "Critical Assessment of Mycotoxins in Beverages and Their Control Measures." Toxins 13, no. 5: 323.
The risk of degenerative diseases decreases with the consumption of high antioxidant containing fruits. This study aimed to analyze the proximate composition and antioxidant activity of pulp and seed of Syzygium cumini. Proximate composition was determined by following the AOAC method. Minerals and heavy metals were analyzed using a flame photometer and an atomic absorption spectrometer. In-vitro antioxidant activity was evaluated by using phosphomolybdenum, ferrous ion chelating ability assay and reducing power assay methods. In proximate composition analysis pulp contained significantly (p
S. Ahmed; K.S. Ahmed; M.S. Hossain; M.S. Azam; M. Rahman; M. Hoque. Proximate composition and antioxidant activity of Syzygium cumini fruit grown at different regions in Bangladesh. Food Research 2020, 4, 1693 -1699.
AMA StyleS. Ahmed, K.S. Ahmed, M.S. Hossain, M.S. Azam, M. Rahman, M. Hoque. Proximate composition and antioxidant activity of Syzygium cumini fruit grown at different regions in Bangladesh. Food Research. 2020; 4 (5):1693-1699.
Chicago/Turabian StyleS. Ahmed; K.S. Ahmed; M.S. Hossain; M.S. Azam; M. Rahman; M. Hoque. 2020. "Proximate composition and antioxidant activity of Syzygium cumini fruit grown at different regions in Bangladesh." Food Research 4, no. 5: 1693-1699.
Zearalenone (ZEA) is an estrogenic and ochratoxin A (OTA) is a hepatotoxic Fusarium mycotoxin commonly seen in cereals and fruits products. No previous investigation has studied on a single platform for the multi degradation mycotoxin. The current study aimed to investigate the bifunctional activity of a novel fusion recombinant. We have generated a recombinant fusion enzyme (ZHDCP) by combining two single genes named zearalenone hydrolase (ZHD) and carboxypeptidase (CP) in frame deletion by crossover polymerase chain reaction (PCR). We identified enzymatic properties and cell cytotoxicity assay of ZHDCP enzyme. Our findings have demonstrated that ZEA was completely degraded to the non-toxic product in 2 h by ZHDCP enzyme at an optimum pH of 7 and a temperature of 35 °C. For the first time, it was found out that ZEA 60% was degraded by CP degrades in 48 h. Fusion ZHDCP and CP enzyme were able to degrade 100% OTA in 30 min at pH 7 and temperature 30 °C. ZEA- and OTA-induced cell death and increased cell apoptosis rate and regulated mRNA expression of Sirt1, Bax, Bcl2, Caspase3, TNFα, and IL6 genes. Our novel findings demonstrated that the fusion enzyme ZHDCP possess bifunctional activity (degrade OTA and ZEA), and it could be used to degrade more mycotoxins.
Shofiul Azam; Dianzhen Yu; Na Liu; Aibo Wu. Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme. Toxins 2019, 11, 301 .
AMA StyleShofiul Azam, Dianzhen Yu, Na Liu, Aibo Wu. Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme. Toxins. 2019; 11 (5):301.
Chicago/Turabian StyleShofiul Azam; Dianzhen Yu; Na Liu; Aibo Wu. 2019. "Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme." Toxins 11, no. 5: 301.
Emerging evidence implies a key role of angiopoietin-like protein 8 (Angptl8) in the metabolic transition between fasting and feeding, whereas much less is known about the mechanism of its own expression. Here we show that hepatic Angptl8 is rhythmically expressed, which involving the liver X receptor alpha (LXRα) and glucocorticoid receptor (GR) modulation during feeding and fasting periods, respectively. In addition, Angptl8 mRNA is very unstable, which contributes to the nature of its daily rhythmicity by rapidly responding to fasting/feeding transition. To explore its pathological function in dexamethasone (DEX)-induced fatty liver, we reversed its suppression by glucocorticoids through adenoviral delivery of Angptl8 gene in mouse liver. Surprisingly, hepatic overexpression of Angptl8 dramatically elevated plasma triglyceride (TG) and non-esterified fatty acid (NEFA) levels in DEX-treated mice, suggesting a metabolic interaction between Angptl8 and glucocorticoid signaling. Moreover, intracellular hepatic Angptl8 is implicated in the regulation of lipid homeostasis by the experiments with ectopic expression of a nonsecreted Angptl8 mutant (Δ25-Angptl8). Altogether, our data demonstrate the molecular mechanism of the diurnal rhythm of Angptl8 expression regulated by glucocorticoid signaling and LXRα pathway, and provide new evidence to understand the role of Angptl8 in maintaining plasma TG homeostasis.
Fabin Dang; Rong Wu; Pengfei Wang; Yuting Wu; Shofiul Azam; Qian Xu; YaQiong Chen; Yi Liu. Fasting and Feeding Signals Control the Oscillatory Expression of Angptl8 to Modulate Lipid Metabolism. Scientific Reports 2016, 6, 36926 .
AMA StyleFabin Dang, Rong Wu, Pengfei Wang, Yuting Wu, Shofiul Azam, Qian Xu, YaQiong Chen, Yi Liu. Fasting and Feeding Signals Control the Oscillatory Expression of Angptl8 to Modulate Lipid Metabolism. Scientific Reports. 2016; 6 (1):36926.
Chicago/Turabian StyleFabin Dang; Rong Wu; Pengfei Wang; Yuting Wu; Shofiul Azam; Qian Xu; YaQiong Chen; Yi Liu. 2016. "Fasting and Feeding Signals Control the Oscillatory Expression of Angptl8 to Modulate Lipid Metabolism." Scientific Reports 6, no. 1: 36926.
The screening method for discovering new lipid synthesis inhibitors has not been well developed. Lipid synthesis genes are responsible for the synthesis of fatty acids in normal and tumor cells. Mortierella alpina(M. alpina) fungus has been found to produce large amounts of fatty acids because it possesses a full complement of lipid synthesis genes. We therefore hypothesized that M. alpina could be a good screening tool to find new compounds that inhibit fatty acid synthesis. We developed a M. alpina liquid culture based method to analyze the inhibitory effects of lipid synthesis inhibitors. We applied a color indicator method to monitor the oil production using some well documented lipid inhibitors (C75, Cerulenin) to verify the system, and analyzing pictures using image analysis software. The experimental drug nocodazole inhibited the lipid production in M. alpina almost to the same extent as the control inhibitors. Compared with biomass and protein levels, there was a profound effect on fatty acid level. We report the development of a fast and effective method for screening lipid synthesis inhibitors which can be used against obesity and cancer. This method can further be used to screen additional lipid and fatty acid synthesis inhibitors from natural compound libraries.
Shofiul Azam; Zhennan Gu; Haiqin Chen; Yong Q. Chen. Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening. Advances in Bioscience and Bioengineering 2015, 3, 1 .
AMA StyleShofiul Azam, Zhennan Gu, Haiqin Chen, Yong Q. Chen. Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening. Advances in Bioscience and Bioengineering. 2015; 3 (1):1.
Chicago/Turabian StyleShofiul Azam; Zhennan Gu; Haiqin Chen; Yong Q. Chen. 2015. "Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening." Advances in Bioscience and Bioengineering 3, no. 1: 1.