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Genetic diversity and structure of common bean (Phaseolus vulgaris L.) germplasm from Serbia, comprising 118 landraces and 18 cultivars, was assessed with the application of 27 Single Sequence Repeats (SSR) markers. Thirteen accessions from Agricultural Institute of Slovenia were used as references for gene pool determination. Main parameters of genetic diversity were calculated for each SSR loci, i.e. number of different and rare alleles, number of effective alleles, Shannon's information index, observed and expected heterozygosity and polymorphic information content. A total of 445 allelic variants, with 16.5 alleles per locus on average, were detected. Mean gene diversity (He = 0.79) indicated sufficient reservoir of genetic variation preserved in studied bean germplasm. Landraces displayed higher variability compared to cultivars (405 in relation to 233 allelic variants). Genetic structure and relatedness of accessions was assessed by model-based method and hierarchical clustering method in combination with genetic distance calculation. The Bayesian clustering model implemented in STRUCTURE software, on the primary level (K = 2), revealed clear separation of accessions into two groups, corresponding to gene pool affiliation. Mesoamerican gene pool (M) was represented with 23.5% of accessions, while Andean (A) was larger, composed of 68.4% of studied germplasm. Small group (8.1%) showed admixed genetic structure between two gene pools. Additional variation in respect to two recognized gene pools was revealed (K = 3), whose basis was acknowledged to be within Andean gene pool. Further subdivision of accessions (K = 8), mainly according to the seed forms, was observed. Genetic distance analysis associated with Neighbour-joining clustering method revealed grouping pattern of landraces and cultivars corresponding to the gene pool and their seed phenotypes. Classification and structuring of the bean accessions according to and beyond the gene pool of origin should facilitate conservation strategies and breeding of this material. Combining the information of phenotypic variation obtained in previous research and molecular data reveled in this study will assist in selection of parental components for breeding, or in the choice of smaller sample in order to further acknowledge their breeding value. In addition, obtained results of this work should serve as an additional information on common bean germplasm variation in Western Balkans and beyond, in Europe.
Aleksandra Savić; Barbara Pipan; Mirjana Vasić; Vladimir Meglič. Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR). Scientia Horticulturae 2021, 288, 110405 .
AMA StyleAleksandra Savić, Barbara Pipan, Mirjana Vasić, Vladimir Meglič. Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR). Scientia Horticulturae. 2021; 288 ():110405.
Chicago/Turabian StyleAleksandra Savić; Barbara Pipan; Mirjana Vasić; Vladimir Meglič. 2021. "Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR)." Scientia Horticulturae 288, no. : 110405.
Buckwheat is a nutritionally valuable crop, an alternative to common cereals also usable in gluten-free diets. The selection of buckwheat genotypes suitable for further breeding requires the characterization and evaluation of genetic resources. The main objective of this work was to evaluate selected phenotypic and morphological traits using international buckwheat descriptors, including total phenolic content and antioxidant activity, on a unique set of 136 common buckwheat accessions grown in 2019–2020 under Czech Republic conditions. In addition, UHPLC-ESI- MS/MS was used to analyze a wide spectrum of 20 phenolic compounds in buckwheat seeds, including four flavanols, three phenolic acids, seven flavonols, four flavones, and two flavanones. Significant differences among years and genotypes were observed for morphological traits (plant height and 1000-seed weight) and antioxidant activity, as well as levels of observed chemical compounds. Antioxidant activity, crude protein content, plant height and rutin content were characterized by higher mean values in 2020 than in 2019 and vice versa for total polyphenol content and 1000-seed weight. Crude protein content was the most stable across years, while total polyphenol content and rutin content varied greatly from year to year. The most abundant phenolic compounds were rutin, hyperoside, epicatechin, catechin, vitexin, isovitexin, orientin and isoorientin. Protein content was negatively correlated with plant height, catechin and epicatechin content. On the other hand, AA and TPC were positively correlated with rutin, hyperoside and chlorogenic acid. Five accessions showed high stability of the evaluated traits under changing conditions within both years of observation. These materials can be used in breeding programmes aimed at improving buckwheat genotypes with emphasis on quality traits.
Dagmar Janovská; Michal Jágr; Pavel Svoboda; Václav Dvořáček; Vladimir Meglič; Petra Hlásná Čepková. Breeding Buckwheat for Nutritional Quality in the Czech Republic. Plants 2021, 10, 1262 .
AMA StyleDagmar Janovská, Michal Jágr, Pavel Svoboda, Václav Dvořáček, Vladimir Meglič, Petra Hlásná Čepková. Breeding Buckwheat for Nutritional Quality in the Czech Republic. Plants. 2021; 10 (7):1262.
Chicago/Turabian StyleDagmar Janovská; Michal Jágr; Pavel Svoboda; Václav Dvořáček; Vladimir Meglič; Petra Hlásná Čepková. 2021. "Breeding Buckwheat for Nutritional Quality in the Czech Republic." Plants 10, no. 7: 1262.
Background Tartary buckwheat (Fagopyrum tataricum) is a nutritionally balanced and flavonoid-rich crop plant that has been in cultivation for 4000 years and is now grown globally. Despite its nutraceutical and agricultural value, the characterization of its genetics and its domestication history is limited. Results Here, we report a comprehensive database of Tartary buckwheat genomic variation based on whole-genome resequencing of 510 germplasms. Our analysis suggests that two independent domestication events occurred in southwestern and northern China, resulting in diverse characteristics of modern Tartary buckwheat varieties. Genome-wide association studies for important agricultural traits identify several candidate genes, includingFtUFGT3andFtAP2YT1that significantly correlate with flavonoid accumulation and grain weight, respectively. Conclusions We describe the domestication history of Tartary buckwheat and provide a detailed resource of genomic variation to allow for genomic-assisted breeding in the improvement of elite cultivars.
Kaixuan Zhang; Ming He; Yu Fan; Hui Zhao; Bin Gao; Keli Yang; Faliang Li; Yu Tang; Qiang Gao; Tao Lin; Muriel Quinet; Dagmar Janovská; Vladimir Meglič; Jacek Kwiatkowski; Olga Romanova; Nikhil Chrungoo; Tatsuro Suzuki; Zlata Luthar; Mateja Germ; Sun-Hee Woo; Milen I. Georgiev; Meiliang Zhou. Resequencing of global Tartary buckwheat accessions reveals multiple domestication events and key loci associated with agronomic traits. Genome Biology 2021, 22, 1 -17.
AMA StyleKaixuan Zhang, Ming He, Yu Fan, Hui Zhao, Bin Gao, Keli Yang, Faliang Li, Yu Tang, Qiang Gao, Tao Lin, Muriel Quinet, Dagmar Janovská, Vladimir Meglič, Jacek Kwiatkowski, Olga Romanova, Nikhil Chrungoo, Tatsuro Suzuki, Zlata Luthar, Mateja Germ, Sun-Hee Woo, Milen I. Georgiev, Meiliang Zhou. Resequencing of global Tartary buckwheat accessions reveals multiple domestication events and key loci associated with agronomic traits. Genome Biology. 2021; 22 (1):1-17.
Chicago/Turabian StyleKaixuan Zhang; Ming He; Yu Fan; Hui Zhao; Bin Gao; Keli Yang; Faliang Li; Yu Tang; Qiang Gao; Tao Lin; Muriel Quinet; Dagmar Janovská; Vladimir Meglič; Jacek Kwiatkowski; Olga Romanova; Nikhil Chrungoo; Tatsuro Suzuki; Zlata Luthar; Mateja Germ; Sun-Hee Woo; Milen I. Georgiev; Meiliang Zhou. 2021. "Resequencing of global Tartary buckwheat accessions reveals multiple domestication events and key loci associated with agronomic traits." Genome Biology 22, no. 1: 1-17.
Understanding the genetic background of drought tolerance in common bean (Phaseolus vulgaris L.) can aid its resilience improvement. However, drought response studies in large seeded genotypes of Andean origin are insufficient. Here, a novel Andean intra-gene pool genetic linkage map was created for quantitative trait locus (QTL) mapping of drought-responsive traits in a recombinant inbred line population from a cross of two cultivars differing in their response to drought. Single environment and QTL × environment analysis revealed 49 QTLs for physiology, phenology, and yield-associated traits under control and/or drought conditions. Notable QTLs for days to flowering (Df1.1 and Df 1.2) were co-localized with a putative QTL for days to pods (Dp1.1) on linkage group 1, suggesting pleiotropy for genes controlling them. QTLs with stable effects for number of seeds per pod (Sp2.1) in both seasons and putative water potential QTLs (Wp1.1, Wp5.1) were detected. Detected QTLs were validated by projection on common bean consensus linkage map. Drought response-associated QTLs identified in the novel Andean recombinant inbred line (RIL) population confirmed the potential of Andean germplasm in improving drought tolerance in common bean. Yield-associated QTLs Syp1.1, Syp1.2, and Sp2.1 in particular could be useful for marker-assisted selection for higher yield of Andean common beans.
Aleš Sedlar; Mateja Zupin; Marko Maras; Jaka Razinger; Jelka Šuštar-Vozlič; Barbara Pipan; Vladimir Meglič. QTL Mapping for Drought-Responsive Agronomic Traits Associated with Physiology, Phenology, and Yield in an Andean Intra-Gene Pool Common Bean Population. Agronomy 2020, 10, 225 .
AMA StyleAleš Sedlar, Mateja Zupin, Marko Maras, Jaka Razinger, Jelka Šuštar-Vozlič, Barbara Pipan, Vladimir Meglič. QTL Mapping for Drought-Responsive Agronomic Traits Associated with Physiology, Phenology, and Yield in an Andean Intra-Gene Pool Common Bean Population. Agronomy. 2020; 10 (2):225.
Chicago/Turabian StyleAleš Sedlar; Mateja Zupin; Marko Maras; Jaka Razinger; Jelka Šuštar-Vozlič; Barbara Pipan; Vladimir Meglič. 2020. "QTL Mapping for Drought-Responsive Agronomic Traits Associated with Physiology, Phenology, and Yield in an Andean Intra-Gene Pool Common Bean Population." Agronomy 10, no. 2: 225.
Background Chicory (Cichorium intybus L.) is a traditional European crop that is highly appreciated for its contents of bioactive compounds, especially phenolics, which have high antioxidant activities. Among other factors, agricultural practice might affect the contents of these bioactive compounds, which are also important from a nutritional point of view, and affect the shelf-life. Results The antioxidant potential (AOP) of chicory plants treated with different fertilisers was investigated in vitro using DPPH radical scavenging and in vivo using the yeast Saccharomyces cerevisiae. Additionally, total phenolics content (TPC) was evaluated using Folin–Ciocalteu reagent, and total flavonoids content (TFC) using the aluminium chloride method. Four different chicory cultivars were included: ‘Treviso’, ‘Verona’ and ‘Anivip’ as red cultivars; and ‘Castelfranco’ as a red-spotted cultivar. These were grown in pots under controlled glasshouse conditions using organic and/or mineral fertilisers. The combination of organic and mineral fertilisers during red chicory growth resulted in significantly higher in-vitro and in-vivo AOPs compared to the control. For the red-spotted cultivar ‘Castelfranco’, this combined organic and mineral fertilisation decreased AOPs in vitro and increased AOPs in vivo. Among the cultivars examined, ‘Castelfranco’ treated with combined organic plus mineral fertilisers showed the highest AOP in vivo, accompanied by the lowest TPC and TFC. Conclusions These data show that application of different fertilisers has different impacts on red and red-spotted chicory cultivars in terms of TFC and TPC, which for red-spotted chicory resulted in different AOPs in vitro and in vivo. The in-vitro AOP is well reflected in the in-vivo AOP for the red chicory cultivars, but less so for the red-spotted cultivar ‘Castelfranco’. Based on the in-vivo AOPs for these chicory cultivars analysed, the combined organic plus mineral fertiliser treatment is recommended.
Lovro Sinkovič; Polona Jamnik; Mojca Korošec; Rajko Vidrih; Vladimir Meglič. In-vitro and in-vivo antioxidant assays of chicory plants (Cichorium intybus L.) as influenced by organic and conventional fertilisers. BMC Plant Biology 2020, 20, 1 -10.
AMA StyleLovro Sinkovič, Polona Jamnik, Mojca Korošec, Rajko Vidrih, Vladimir Meglič. In-vitro and in-vivo antioxidant assays of chicory plants (Cichorium intybus L.) as influenced by organic and conventional fertilisers. BMC Plant Biology. 2020; 20 (1):1-10.
Chicago/Turabian StyleLovro Sinkovič; Polona Jamnik; Mojca Korošec; Rajko Vidrih; Vladimir Meglič. 2020. "In-vitro and in-vivo antioxidant assays of chicory plants (Cichorium intybus L.) as influenced by organic and conventional fertilisers." BMC Plant Biology 20, no. 1: 1-10.
Drought is one of the major threats to common bean (Phaseolus vulgaris L.), affecting its growth and productivity and, thus, contributing to considerable losses in yield in many regions worldwide. The development of varieties tolerant of drought stress has, therefore, become one of the primary goals in many common bean breeding programs. Plants have developed various mechanisms of their adaptation, to a greater or lesser extent, to drought. These are expressed, on the molecular level, by changes of gene expression and of protein content, together with responses at physiological and morphological levels. The response of common bean to drought is still not sufficiently well characterized due to its genetic complexity and its diverse, often ambiguous, phenotypic effects. Understanding these mechanisms is thus of fundamental importance for developing varieties that are better adapted to such stress conditions. In this chapter, we present research that provides an insight into the morpho-physiological adaptation and its underlying molecular changes in common bean plants exposed to drought. We include our contribution to establishing the basis for breeding of common bean with greater tolerance to this abiotic stress that uses molecular markers and identification of quantitative trait loci (QTLs).
Aleš Sedlar; Marjetka Kidrič; Jelka Šuštar-Vozlič; Barbara Pipan; Tanja Zadražnik; Vladimir Meglič. Drought Stress Response in Agricultural Plants: A Case Study of Common Bean (Phaseolus vulgaris L.). Drought - Detection and Solutions 2020, 1 .
AMA StyleAleš Sedlar, Marjetka Kidrič, Jelka Šuštar-Vozlič, Barbara Pipan, Tanja Zadražnik, Vladimir Meglič. Drought Stress Response in Agricultural Plants: A Case Study of Common Bean (Phaseolus vulgaris L.). Drought - Detection and Solutions. 2020; ():1.
Chicago/Turabian StyleAleš Sedlar; Marjetka Kidrič; Jelka Šuštar-Vozlič; Barbara Pipan; Tanja Zadražnik; Vladimir Meglič. 2020. "Drought Stress Response in Agricultural Plants: A Case Study of Common Bean (Phaseolus vulgaris L.)." Drought - Detection and Solutions , no. : 1.
In South-Eastern Europe, the majority of runner-bean (Phaseolus coccineus L.) production is based on local populations grown mainly in home gardens. The local runner-bean plants are well adapted to their specific growing conditions and microclimate agro-environments, and show great morpho-agronomic diversity. Here, 142 runner-bean accessions from the five South-Eastern European countries of Slovenia, Bosnia and Herzegovina, Serbia, North Macedonia and Romania were sown and cultivated in their respective countries and characterised using 28 quantitative and qualitative morpho-agronomic descriptors for Phaseolus spp. based on inflorescences, leaves, plants, pods and seeds. For each of these morpho-agronomic descriptors, the accessions can be classified into two or three specific groups. The highest correlations were observed within the fluorescence, seed and pod traits. The highest variability, at 76.39%, was between the different countries, representing different geographic origins, while the variability within the countries was 23.61%. Cluster analysis based on these collected morpho-agronomic data also classified the accessions into three groups according to genetic origins. The data obtained serve as useful genetic information for plant breeders for the breeding of new bean varieties for further studies of the morpho-agronomic traits of the runner bean.
Lovro Sinkovič; Barbara Pipan; Mirjana Vasić; Marina Antić; Vida Todorović; Sonja Ivanovska; Creola Brezeanu; Jelka Šuštar-Vozlič; Vladimir Meglič. Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe. Sustainability 2019, 11, 6165 .
AMA StyleLovro Sinkovič, Barbara Pipan, Mirjana Vasić, Marina Antić, Vida Todorović, Sonja Ivanovska, Creola Brezeanu, Jelka Šuštar-Vozlič, Vladimir Meglič. Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe. Sustainability. 2019; 11 (21):6165.
Chicago/Turabian StyleLovro Sinkovič; Barbara Pipan; Mirjana Vasić; Marina Antić; Vida Todorović; Sonja Ivanovska; Creola Brezeanu; Jelka Šuštar-Vozlič; Vladimir Meglič. 2019. "Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe." Sustainability 11, no. 21: 6165.
Unpredicted persistence of all forms of B. napus present in the agro-ecosystem is the most common consequence of preservation and self-recruitment of seeds originating from soil seed bank. In nature, spontaneous intra- and inter-specific hybridization of B. napus is possible with sexually compatible species from the Brassicaceae family. The aim of this chapter is (a) to identify the distribution pattern and population dynamics of volunteers and feral populations along statistical regions in Slovenia; (b) to assess the global diversity of naturally appearing B. napus plants; (c) to evaluate the genetic differentiation between volunteers and feral populations; (d) to obtain the spatial and temporal distribution of spontaneous pollination potential and estimation of gene flow conservation; (e) to find the empirically assigned out-crossing rate of B. napus under a fragmented landscape structure, during 4-year monitoring; and (f) to observe that ecologically, evolutionary, and agronomically oriented studies could be conducted at the DNA level using short sequence repeat (SSR) markers. In total, we collected 261 samples of volunteer and feral populations. Our results showed that alleles from both volunteer and feral populations were distributed in three genetic clusters with relatively similar levels of diversity. Naturally occurring out-crossing rate is 13.71%. The global Mantel correlation coefficient of genetic and spatial relatedness between genotypes is 0.044.
Vladimir Meglič; Barbara Pipan. Spatial and Temporal Assessment of Brassica napus L. Maintaining Genetic Diversity and Gene Flow Potential: An Empirical Evaluation. Brassica Germplasm - Characterization, Breeding and Utilization 2018, 1 .
AMA StyleVladimir Meglič, Barbara Pipan. Spatial and Temporal Assessment of Brassica napus L. Maintaining Genetic Diversity and Gene Flow Potential: An Empirical Evaluation. Brassica Germplasm - Characterization, Breeding and Utilization. 2018; ():1.
Chicago/Turabian StyleVladimir Meglič; Barbara Pipan. 2018. "Spatial and Temporal Assessment of Brassica napus L. Maintaining Genetic Diversity and Gene Flow Potential: An Empirical Evaluation." Brassica Germplasm - Characterization, Breeding and Utilization , no. : 1.
Dragan Žnidarčič; Filip Vučanjk; Žarko M. Ilin; Barbara Pipan; Vladimir Meglič; Lovro Sinkovič. The Influence of Different Substrates on the Growth, Yield and Quality of Slovenian Sweetpotato Cultivars under Greenhouse Conditions. Vegetables - Importance of Quality Vegetables to Human Health 2018, 1 .
AMA StyleDragan Žnidarčič, Filip Vučanjk, Žarko M. Ilin, Barbara Pipan, Vladimir Meglič, Lovro Sinkovič. The Influence of Different Substrates on the Growth, Yield and Quality of Slovenian Sweetpotato Cultivars under Greenhouse Conditions. Vegetables - Importance of Quality Vegetables to Human Health. 2018; ():1.
Chicago/Turabian StyleDragan Žnidarčič; Filip Vučanjk; Žarko M. Ilin; Barbara Pipan; Vladimir Meglič; Lovro Sinkovič. 2018. "The Influence of Different Substrates on the Growth, Yield and Quality of Slovenian Sweetpotato Cultivars under Greenhouse Conditions." Vegetables - Importance of Quality Vegetables to Human Health , no. : 1.
Drought is one of the major abiotic stress conditions limiting crop growth and productivity. Glycosylation of proteins is very important post-translational modification that is involved in many physiological functions and biological pathways. To understand the involvement of N-glycoproteins in the mechanism of drought response in leaves of common bean, a proteomic approach using lectin affinity chromatography, SDS-PAGE and LC-MS/MS was applied. Quantification of N-glycoproteins was performed using MaxQuant with a label free quantification approach. Thirty five glycoproteins were changed in abundance in leaves of common bean under drought. The majority of these proteins were classified into functional groups that include cell wall processes, defence/stress related proteins and proteins related to proteolysis. Beta-glucosidase showed the highest increase in abundance among proteins involved in cell wall metabolism, suggesting its role in cell wall modification under drought stress. These results fit with the general concept of the stress response in plants and suggest that drought stress might affect biochemical metabolism in the cell wall. The structures of N-glycans were determined manually from spectra, where structures of high mannose, complex and hybrid types of N-glycans were found. The present study provided an insight into the glycoproteins related to drought stress in common bean at the proteome level, which is important for further understanding of molecular mechanisms of drought response in this important legume.
Tanja Zadražnik; Anders Moen; Wolfgang Egge-Jacobsen; Vladimir Meglič; Jelka Šuštar-Vozlič. Towards a better understanding of protein changes in common bean under drought: A case study of N-glycoproteins. Plant Physiology and Biochemistry 2017, 118, 400 -412.
AMA StyleTanja Zadražnik, Anders Moen, Wolfgang Egge-Jacobsen, Vladimir Meglič, Jelka Šuštar-Vozlič. Towards a better understanding of protein changes in common bean under drought: A case study of N-glycoproteins. Plant Physiology and Biochemistry. 2017; 118 ():400-412.
Chicago/Turabian StyleTanja Zadražnik; Anders Moen; Wolfgang Egge-Jacobsen; Vladimir Meglič; Jelka Šuštar-Vozlič. 2017. "Towards a better understanding of protein changes in common bean under drought: A case study of N-glycoproteins." Plant Physiology and Biochemistry 118, no. : 400-412.
Aquaporin proteins are part of the complex response of common bean (Phaseolus vulgaris L.) to drought which affects the quality and quantity of yield of this important crop. To better understand the role of aquaporins in common bean, drought-induced gene expression of several aquaporins was determined in two cultivars, the more drought tolerant Tiber and the less tolerant Starozagorski čern. The two bean cultivars were selected among 16 European genotypes based on the tolerance to drought determined by time needed for plants to wilt after withholding irrigation and yield at harvest. The expression patterns of two plasma membrane intrinsic proteins, PvPIP1;2 and PvPIP2;7, and two tonoplast intrinsic proteins, PvTIP1;1 and PvTIP4;1 in leaves of 21 day old plants were determined by RT-qPCR in both cultivars under three degrees of drought stress, and under rehydration and control conditions. Gene expression of all four examined aquaporins was down-regulated in drought stressed plants. After rehydration it returned to the level of control plants or was even higher. The responses of PvPIP2;7 and PvTIP1;1 during drought and rehydration were particularly pronounced. The gene expression of PvPIP2;7 and PvTIP4;1 during drought was cultivar specific, with greater down-regulation of these two aquaporins in drought tolerant Tiber. Under drought stress the relative water content and water potential of leaves were higher in Tiber than in Starozagorski plants. The differences in these physiological parameters indicate greater prevention of water loss in Tiber during drought, which may be associated with rapid and adequate down-regulation of aquaporins. These results suggest that the ability of plants to conserve water during drought stress involves timely and sufficient down-regulation of gene expression of specific aquaporins.
Mateja Zupin; Aleš Sedlar; Marjetka Kidrič; Vladimir Meglič. Drought-induced expression of aquaporin genes in leaves of two common bean cultivars differing in tolerance to drought stress. Journal of Plant Research 2017, 130, 735 -745.
AMA StyleMateja Zupin, Aleš Sedlar, Marjetka Kidrič, Vladimir Meglič. Drought-induced expression of aquaporin genes in leaves of two common bean cultivars differing in tolerance to drought stress. Journal of Plant Research. 2017; 130 (4):735-745.
Chicago/Turabian StyleMateja Zupin; Aleš Sedlar; Marjetka Kidrič; Vladimir Meglič. 2017. "Drought-induced expression of aquaporin genes in leaves of two common bean cultivars differing in tolerance to drought stress." Journal of Plant Research 130, no. 4: 735-745.
Drought is an abiotic stress that strongly influences plant growth, development and productivity. Proteome changes in the stem of the drought-tolerant common bean (Phaseolus vulgaris L.) cultivar Tiber have were when the plants were exposed to drought. Five-week-old plants were subjected to water deficit by withholding irrigation for 7, 12 and 17days, whereas control plants were regularly irrigated. Relative water content (RWC) of leaves, as an indicator of the degree of cell and tissue hydration, showed the highest statistically significant differences between control and drought-stressed plants after 17days of treatment, where RWC remained at 90% for control and declined to 45% for stressed plants. Plants exposed to drought for 17days and control plants at the same developmental stage were included in quantitative proteomic analysis using in-gel stable isotope labeling of proteins in combination with mass spectrometry. The quantified proteins were grouped into several functional groups, mainly into energy metabolism, photosynthesis, proteolysis, protein synthesis and proteins related to defense and stress. 70kDa heat shock protein showed the greatest increase in abundance under drought of all the proteins, suggesting its role in protecting plants against stress by re-establishing normal protein conformations and thus cellular homeostasis. The abundance of proteins involved in protein synthesis also increased under drought stress, important for recovery of damaged proteins involved in the plant cell's metabolic activities. Other important proteins in this study were related to proteolysis and folding, which are necessary for maintaining proper cellular protein homeostasis. Taken together, these results reveal the complexity of pathways involved in the drought stress response in common bean stems and enable comparison with the results of proteomic analysis of leaves, thus providing important information to further understand the biochemical and molecular mechanisms of drought response in this important legume.
Tanja Zadražnik; Wolfgang Egge-Jacobsen; Vladimir Meglič; Jelka Šuštar-Vozlič. Proteomic analysis of common bean stem under drought stress using in-gel stable isotope labeling. Journal of Plant Physiology 2017, 209, 42 -50.
AMA StyleTanja Zadražnik, Wolfgang Egge-Jacobsen, Vladimir Meglič, Jelka Šuštar-Vozlič. Proteomic analysis of common bean stem under drought stress using in-gel stable isotope labeling. Journal of Plant Physiology. 2017; 209 ():42-50.
Chicago/Turabian StyleTanja Zadražnik; Wolfgang Egge-Jacobsen; Vladimir Meglič; Jelka Šuštar-Vozlič. 2017. "Proteomic analysis of common bean stem under drought stress using in-gel stable isotope labeling." Journal of Plant Physiology 209, no. : 42-50.
The study of reliability of morphological characterization of lightsprouts for differentiation of potato varieties was performed at the Agricultural Institute of Slovenia in cooperation with Biotechnical Faculty Podgorica in order to introduce simple method for further characterization of potato accessions in Montenegrin gene bank. Seven selected, potentially different, potato accessions preserved in the Montenegrin gene bank were used for morphological characterization of lightsprouts. Using UPOV guidelines 11 lightsprout traits were estimated. Molecular assessment was carried out in parallel with morphological characterization by six microsatellite (SSR) markers. The latter successfully distinguished all accessions but two, while four different lightsprout phenotypes were identified in morphological characterization. Though molecular markers showed more strength in resolving relationships between genotypes, characterization of lightsprouts still demonstrated its usefulness due to cheap, simple and rapid procedure.
Peter Dolnicar; Drago Milosevic; Zoran Jovovic; Vladimir Meglic; Marko Maras; Ana Velimirovic. Reliability of morphological and molecular characterization of lightsprouts for differentiation of potato accessions. Genetika 2016, 48, 525 -532.
AMA StylePeter Dolnicar, Drago Milosevic, Zoran Jovovic, Vladimir Meglic, Marko Maras, Ana Velimirovic. Reliability of morphological and molecular characterization of lightsprouts for differentiation of potato accessions. Genetika. 2016; 48 (2):525-532.
Chicago/Turabian StylePeter Dolnicar; Drago Milosevic; Zoran Jovovic; Vladimir Meglic; Marko Maras; Ana Velimirovic. 2016. "Reliability of morphological and molecular characterization of lightsprouts for differentiation of potato accessions." Genetika 48, no. 2: 525-532.
Cultivation of common bean has a long tradition in the Former Yugoslav Republic of Macedonia (FYROM) and is still nowadays important part of the human diet. In a study reported here 71 accessions from the FYROM were assessed for genetic diversity with the aim to provide information on genetic structure of Macedonian common bean germplasm and to depict its peculiarities. A total of 71 accessions were assessed using 13 microsatellite and 16 morphological markers. The average number of alleles per microsatellite was 5.8, and ranged from three to 16 alleles. High capacity of selected markers for distinguishing genotypes was identified by the calculation of a very low value of probability of identity. The relationship among 71 studied accessions was assessed by hierarchical cluster analysis. A very clear separation of accessions into two groups was observed in the UPGMA dendrogram. The larger represented Andean gene pool and contained 40 accessions (56% of total), while the other 31 accessions (44% of total) composed Mesoamerican gene pool. The two groups were successfully discriminated by eight morphological traits. Within the larger Andean cluster in the UPGMA dendrogram a sub-group of 16 climbing accessions was separated from 24 bush accessions. The absence of the string in the pods of the climbers suggests that this sub-group comprises snap beans grown primarily for their fresh pods. There were eight morphological traits in total that distinguished the two Andean sub-groups. Assessment of genetic relationship among accessions, their classification into respective gene pool and identification of morphological peculiarities provided valuable information for the management of plant gene bank and Macedonian bean breeding program.
Marko Maras; Afrodita Ibusoska; Suzana Kratovalieva; Rukie Agic; Jelka Sustar-Vozlic; Vladimir Meglic. Genetic diversity of common bean accessions from former Yugoslav Republic of Macedonia as revealed by molecular and morphological markers. Genetika 2016, 48, 729 -742.
AMA StyleMarko Maras, Afrodita Ibusoska, Suzana Kratovalieva, Rukie Agic, Jelka Sustar-Vozlic, Vladimir Meglic. Genetic diversity of common bean accessions from former Yugoslav Republic of Macedonia as revealed by molecular and morphological markers. Genetika. 2016; 48 (2):729-742.
Chicago/Turabian StyleMarko Maras; Afrodita Ibusoska; Suzana Kratovalieva; Rukie Agic; Jelka Sustar-Vozlic; Vladimir Meglic. 2016. "Genetic diversity of common bean accessions from former Yugoslav Republic of Macedonia as revealed by molecular and morphological markers." Genetika 48, no. 2: 729-742.
Protein breakdown by proteases is basic to the plant response to abiotic stresses such as drought. A large number of genes encoding proteases or putative proteases exist in plants. Only a few of those involved in the response to drought have been characterized, and their regulation is poorly understood. We have identified two new subtilases from leaves of Phaseolus vulgaris L. cultivar Zorin, PvSLP1 and PvSLP2. PvSLP1 was identified at the gene level, using primers based on the gene sequence of the putative drought induced serine protease from Arachis hypogaea L. In P. vulgaris, expression of the PvSLP1 transcript did not change on water withdrawal. PvSLP2 was isolated and characterized at the protein level, together with complete gene and cDNA sequences. The deduced amino acid sequences of both PvSLP1 and PvSLP2 are characteristic of plant subtilases of the S8 family of clan SB. PvSLP2 shows 33% sequence identity to PvSLP1. Expression of the PvSLP2 transcript did not change on withdrawal of water, but its proteolytic activity in leaves increased, depending on the age and position of the leaf. In addition, the level of activity in senescent leaves of well watered plants was higher than in mature or young leaves. These results, together with the fact that PvSLP2 cleaves peptide bonds following an Arg residue, point to regulation of PvSLP2 subtilase activity at translational and/or post-translational levels and suggest a specific role in the response to drought and senescence.
Maruška Budič; Jerica Sabotič; Vladimir Meglič; Janko Kos; Marjetka Kidrič. Characterization of two novel subtilases from common bean (Phaseolus vulgaris L.) and their responses to drought. Plant Physiology and Biochemistry 2013, 62, 79 -87.
AMA StyleMaruška Budič, Jerica Sabotič, Vladimir Meglič, Janko Kos, Marjetka Kidrič. Characterization of two novel subtilases from common bean (Phaseolus vulgaris L.) and their responses to drought. Plant Physiology and Biochemistry. 2013; 62 ():79-87.
Chicago/Turabian StyleMaruška Budič; Jerica Sabotič; Vladimir Meglič; Janko Kos; Marjetka Kidrič. 2013. "Characterization of two novel subtilases from common bean (Phaseolus vulgaris L.) and their responses to drought." Plant Physiology and Biochemistry 62, no. : 79-87.
The majority of common bean plants are cultivated under drought conditions. Maintaining crop yields under drought stress is thus one of the biggest challenges facing bean production. In order to improve our understanding of the complex mechanisms involved in the response of common bean (Phaseolus vulgaris) to drought stress, a proteomic approach was used to identify drought-responsive proteins in leaves of two cultivars differing in their response to drought, Tiber and more sensitive Starozagorski čern. 2D-DIGE was used to compare differences in protein abundance between control and stressed plants. Fifty-eight proteins whose abundance changed significantly were identified by LC-MS/MS in Tiber and 64 in Starozagorski čern. The majority of identified proteins were classified into functional categories that include energy metabolism, photosynthesis, ATP interconversion, protein synthesis and proteolysis, stress and defence related proteins. Details of the function of the identified proteins and their abundance profiles in Tiber and Starozagorski are discussed. Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways and molecular functions affected by drought stress. The results form the basis for a further understanding of the biochemical mechanisms of drought response in common bean.
Tanja Zadražnik; Kristin Hollung; Wolfgang Egge-Jacobsen; Vladimir Meglič; Jelka Šuštar-Vozlič. Differential proteomic analysis of drought stress response in leaves of common bean (Phaseolus vulgaris L.). Journal of Proteomics 2013, 78, 254 -272.
AMA StyleTanja Zadražnik, Kristin Hollung, Wolfgang Egge-Jacobsen, Vladimir Meglič, Jelka Šuštar-Vozlič. Differential proteomic analysis of drought stress response in leaves of common bean (Phaseolus vulgaris L.). Journal of Proteomics. 2013; 78 ():254-272.
Chicago/Turabian StyleTanja Zadražnik; Kristin Hollung; Wolfgang Egge-Jacobsen; Vladimir Meglič; Jelka Šuštar-Vozlič. 2013. "Differential proteomic analysis of drought stress response in leaves of common bean (Phaseolus vulgaris L.)." Journal of Proteomics 78, no. : 254-272.
Tatjana Kavar; Marko Maras; Marjetka Kidrič; Jelka Šuštar-Vozlič; Vladimir Meglič. THE EXPRESSION PROFILES OF SELECTED GENES IN DIFFERENT BEAN SPECIES (PHASEOLUS SPP.) AS RESPONSE TO WATER DEFICIT. Journal of Central European Agriculture 2011, 12, 557 -576.
AMA StyleTatjana Kavar, Marko Maras, Marjetka Kidrič, Jelka Šuštar-Vozlič, Vladimir Meglič. THE EXPRESSION PROFILES OF SELECTED GENES IN DIFFERENT BEAN SPECIES (PHASEOLUS SPP.) AS RESPONSE TO WATER DEFICIT. Journal of Central European Agriculture. 2011; 12 (4):557-576.
Chicago/Turabian StyleTatjana Kavar; Marko Maras; Marjetka Kidrič; Jelka Šuštar-Vozlič; Vladimir Meglič. 2011. "THE EXPRESSION PROFILES OF SELECTED GENES IN DIFFERENT BEAN SPECIES (PHASEOLUS SPP.) AS RESPONSE TO WATER DEFICIT." Journal of Central European Agriculture 12, no. 4: 557-576.
Petra Kozjak; Jelka Šuštar-Vozlič; Vladimir Meglič. Adventitious presence of GMOs in maize in the view of coexistence. Acta agriculturae Slovenica 2011, 97, 275 -284.
AMA StylePetra Kozjak, Jelka Šuštar-Vozlič, Vladimir Meglič. Adventitious presence of GMOs in maize in the view of coexistence. Acta agriculturae Slovenica. 2011; 97 (3):275-284.
Chicago/Turabian StylePetra Kozjak; Jelka Šuštar-Vozlič; Vladimir Meglič. 2011. "Adventitious presence of GMOs in maize in the view of coexistence." Acta agriculturae Slovenica 97, no. 3: 275-284.
Hbr markers are based on location presence/absence of the Heartbreaker family of miniature inverted repeat transposable elements (MITEs). Together with the cost-effective technique--Hbr display were developed in 2000. We chose 15 populations from the Slovenian maize germplasm bank and described ten individual samples per each population by 268 Hbr markers and 35 morphological traits (IPGRI descriptors). Samples from the same population had highly similar DNA fingerprints, while the between populations differences were very high. Therefore, only a minor part of the total genetic variance existed within populations (23.3%), and the major part among populations (76.7%). Beli zob (the only dent type population) and stajerski dvanajsterec were the most divergent populations, others were closely related. They shared the majority of bands in the way that each band was shared by different set of populations. This is suggesting the origin from the common gene pool and the high extent of migrations.
T. Kavar; V. Meglič; L. Rozman. Diversity of Slovenian maize (Zea mays) populations by Hbr (MITE) markers and morphological traits. Генетика 2007, 43, 989 -995.
AMA StyleT. Kavar, V. Meglič, L. Rozman. Diversity of Slovenian maize (Zea mays) populations by Hbr (MITE) markers and morphological traits. Генетика. 2007; 43 (9):989-995.
Chicago/Turabian StyleT. Kavar; V. Meglič; L. Rozman. 2007. "Diversity of Slovenian maize (Zea mays) populations by Hbr (MITE) markers and morphological traits." Генетика 43, no. 9: 989-995.
International audienceThe genetic structure of Apis mellifera carnica bee from Slovenia, collected from 269 localities of ten Slovenian districts, was assessed by mitochondrial and nuclear DNA analyses. The level of genetic variability within and among districts was low. All of the samples were fixed for one newly found mtDNA haplotype of the C phylogenetic lineage, designated as C2C. A low level of variability was observed for all microsatellite loci, showing a very homogenous structure of the Carniolan bee population. Samples collected in the neighbouring district of Croatia expressed very similar results. On the other hand, high genetic differentiation was observed in comparison with A. m. macedonica population. Present study represents the first attempt to characterise indigenous honeybee populations in Slovenia using molecular methods. Furthermore, it indicates that the Carniolan bee from Slovenia still represents an indigenous gene pool within A. m. carnica
Simona Sušnik; Peter Kozmus; Janez Poklukar; Vladimir Meglic. Molecular characterisation of indigenous Apis mellifera carnica in Slovenia. Apidologie 2004, 35, 623 -636.
AMA StyleSimona Sušnik, Peter Kozmus, Janez Poklukar, Vladimir Meglic. Molecular characterisation of indigenous Apis mellifera carnica in Slovenia. Apidologie. 2004; 35 (6):623-636.
Chicago/Turabian StyleSimona Sušnik; Peter Kozmus; Janez Poklukar; Vladimir Meglic. 2004. "Molecular characterisation of indigenous Apis mellifera carnica in Slovenia." Apidologie 35, no. 6: 623-636.