• The pathogenicities of the two biotypes of feline coronavirus differ. • Internal mutation occurs in the two biotypes of feline coronavirus. • Effusive and noneffusive FIP can be transformed mutually based on immune status.

The spliceosome is a large ribonucleoprotein complex that removes introns from pre-mRNAs. At its functional core lies the essential pre-mRNA processing factor 8 (Prp8) protein. Across diverse eukaryotes, this protein cofactor of RNA catalysis harbors a self-splicing element called an intein. Inteins in Prp8 are extremely pervasive and are found at 7 different sites in various species. Here, we focus on the Prp8 intein from Cryptococcus neoformans (Cne), a human fungal pathogen. We solved the crystal structure of this intein, revealing structural homology among protein splicing sequences in eukaryotes, including the Hedgehog C terminus. Working with the Cne Prp8 intein in a reporter assay, we find that the biologically relevant divalent metals copper and zinc inhibit intein splicing, albeit by 2 different mechanisms. Copper likely stimulates reversible modifications on a catalytically important cysteine, whereas zinc binds at the terminal asparagine and the same critical cysteine. Importantly, we also show that copper treatment inhibits Prp8 protein splicing in Cne. Lastly, an intein-containing Prp8 precursor model is presented, suggesting that metal-induced protein-splicing inhibition would disturb function of both Prp8 and the spliceosome. These results indicate that Prp8 protein splicing can be modulated, with potential functional implications for the spliceosome.

Porcine circovirus (PCV) belongs to the genus Circovirus within the family Circoviridae; it has the smallest genome and a complicated classification system comprising PCV1, PCV2, PCV3, and PCV4. Most types of these viruses can cause animals to develop serious diseases; in pigs in particular, it may manifest as postweaning multisystemic wasting syndrome (PMWS), reproductive failure, porcine dermatitis and nephropathy syndrome (PDNS), congenital tremors (CTs), proliferative and necrotizing pneumonia (PNP), lymphoid injury, and immunosuppression. Different types of PCVs cause different types of diseases and sometimes feature no pathogenicity; these various PCV types are associated with different pathomechanisms in animals. In this review, the genomic composition and systemic pathomechanisms of porcine circoviruses are introduced, and future research prospects are discussed.