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Korean traditional food (KTF), originated from ancestral agriculture and the nomadic traditions of the Korean peninsula and southern Manchuria, is based on healthy food that balances disease prevention and treatment. Fermented foods that include grains, herbs, fruits, and mushrooms are also an important practice in KTF, providing high levels of Lactobacilli, which confer relevant health benefits, including antiviral properties. Some of these probiotics may also protect against the Influenza virus through the modulation of innate immunity. The emerging of the COVID-19 pandemic, in addition to other diseases of viral origin, and the problems associated with other respiratory disorders, highlight how essential is a healthy eating pattern to strengthen our immune system. Key Findings and Conclusions: The present review covers the information available on edible plants, herbs, mushrooms, and preparations used in KTF to outline their multiple medicinal effects (e.g., antidiabetic, chemopreventive, antioxidative, anti-inflammatory, antibacterial), emphasizing their role and effects on the immune system with an emphasis on modulating properties of the gut microbiota that further support strong respiratory immunity. Potential functional foods commonly used in Korean cuisine such as Kimchi (a mixture of fermented vegetables), Meju, Doenjang, Jeotgal, and Mekgeolli and fermented sauces, among others, are highlighted for their great potential to improve gut-lung immunity. The traditional Korean diet and dietary mechanisms that may target viruses ACE-2 receptors or affect any step of a virus infection pathway that can determine a patient's prognosis are also highlighted. The regular oral intake of bioactive ingredients used in Korean foods can offer protection for some viral diseases, through protective and immunomodulatory effects, as evidenced in pre-clinical and clinical studies.
Gitishree Das; J. Basilio Heredia; Maria De Lourdes Pereira; Ericsson Coy-Barrera; Sonia Marlene Rodrigues Oliveira; Erick Paul Gutiérrez-Grijalva; Luis Ángel Cabanillas-Bojórquez; Han-Seung Shin; Jayanta Kumar Patra. Korean traditional foods as antiviral and respiratory disease prevention and treatments: A detailed review. Trends in Food Science & Technology 2021, 116, 415 -433.
AMA StyleGitishree Das, J. Basilio Heredia, Maria De Lourdes Pereira, Ericsson Coy-Barrera, Sonia Marlene Rodrigues Oliveira, Erick Paul Gutiérrez-Grijalva, Luis Ángel Cabanillas-Bojórquez, Han-Seung Shin, Jayanta Kumar Patra. Korean traditional foods as antiviral and respiratory disease prevention and treatments: A detailed review. Trends in Food Science & Technology. 2021; 116 ():415-433.
Chicago/Turabian StyleGitishree Das; J. Basilio Heredia; Maria De Lourdes Pereira; Ericsson Coy-Barrera; Sonia Marlene Rodrigues Oliveira; Erick Paul Gutiérrez-Grijalva; Luis Ángel Cabanillas-Bojórquez; Han-Seung Shin; Jayanta Kumar Patra. 2021. "Korean traditional foods as antiviral and respiratory disease prevention and treatments: A detailed review." Trends in Food Science & Technology 116, no. : 415-433.
The fungal pathogen Fusarium oxysporum f. sp. dianthi (Fod) is the causal agent of the vascular wilt of carnation (Dianthus caryophyllus L.) and the most prevalent pathogen in the areas where this flower is grown. For this reason, the development of new control strategies against Fod in carnation has been continuously encouraged, in particular those based on the implementation of plant resistance inducers that can trigger defensive responses to reduce the disease incidence, even at lower economical and environmental cost. In the present study, the effect of the soil supplementation of a biotic elicitor (i.e., ultrasound-assisted dispersion obtained from Fod mycelium) on disease severity and phenolic-based profiles of roots over two carnation cultivars was evaluated. Results suggest that the tested biotic elicitor, namely, eFod, substantially reduced the progress of vascular wilting in a susceptible cultivar (i.e., ‘Mizuki’) after two independent in vivo tests. The LC-MS-derived semi-quantitative levels of phenolic compounds in roots were also affected by eFod, since particular anthranilate derivatives, conjugated benzoic acids, and glycosylated flavonols were upregulated by elicitation after 144 and 240 h post eFod addition. Our findings indicate that the soil-applied eFod has an effect as a resistance inducer, promoting a disease severity reduction and accumulation of particular phenolic-like compounds.
Janneth Santos-Rodríguez; Ericsson Coy-Barrera; Harold Ardila. Mycelium Dispersion from Fusarium oxysporum f. sp. dianthi Elicits a Reduction of Wilt Severity and Influences Phenolic Profiles of Carnation (Dianthus caryophyllus L.) Roots. Plants 2021, 10, 1447 .
AMA StyleJanneth Santos-Rodríguez, Ericsson Coy-Barrera, Harold Ardila. Mycelium Dispersion from Fusarium oxysporum f. sp. dianthi Elicits a Reduction of Wilt Severity and Influences Phenolic Profiles of Carnation (Dianthus caryophyllus L.) Roots. Plants. 2021; 10 (7):1447.
Chicago/Turabian StyleJanneth Santos-Rodríguez; Ericsson Coy-Barrera; Harold Ardila. 2021. "Mycelium Dispersion from Fusarium oxysporum f. sp. dianthi Elicits a Reduction of Wilt Severity and Influences Phenolic Profiles of Carnation (Dianthus caryophyllus L.) Roots." Plants 10, no. 7: 1447.
Alzheimer’s disease is the most common form of dementia, representing 60–70% of dementia cases. The enzyme acetylcholinesterase (AChE) cleaves the ester bonds in acetylcholine and plays an important role in the termination of acetylcholine activity at cholinergic synapses in various regions of the nervous system. The inhibition of acetylcholinesterase is frequently used to treat Alzheimer’s disease. In this study, a merged BindingDB and ChEMBL dataset containing molecules with reported half-maximal inhibitory concentration (IC50) values for AChE (7032 molecules) was used to build machine learning classification models for selecting potential AChE inhibitors from the SistematX dataset (8593 secondary metabolites). A total of seven fivefold models with accuracy above 80% after cross-validation were obtained using three types of molecular descriptors (VolSurf, DRAGON 5.0, and bit-based fingerprints). A total of 521 secondary metabolites (6.1%) were classified as active in this stage. Subsequently, virtual screening was performed, and 25 secondary metabolites were identified as potential inhibitors of AChE. Separately, the crystal structure of AChE in complex with (–)-galantamine was used to perform molecular docking calculations with the entire SistematX dataset. Consensus analysis of both methodologies was performed. Only eight structures achieved combined probability values above 0.5. Finally, two sesquiterpene lactones, structures 15 and 24, were predicted to be able to cross the blood–brain barrier, which was confirmed in the VolSurf+ quantitative model, revealing these two structures as the most promising secondary metabolites for AChE inhibition among the 8593 molecules tested. A consensus analysis of classification models and molecular docking calculations identified four potential inhibitors of acetylcholinesterase from the SistematX dataset (8593 structures).
Chonny Herrera-Acevedo; Camilo Perdomo-Madrigal; Kenyi Herrera-Acevedo; Ericsson Coy-Barrera; Luciana Scotti; Marcus Tullius Scotti. Machine learning models to select potential inhibitors of acetylcholinesterase activity from SistematX: a natural products database. Molecular Diversity 2021, 1 -16.
AMA StyleChonny Herrera-Acevedo, Camilo Perdomo-Madrigal, Kenyi Herrera-Acevedo, Ericsson Coy-Barrera, Luciana Scotti, Marcus Tullius Scotti. Machine learning models to select potential inhibitors of acetylcholinesterase activity from SistematX: a natural products database. Molecular Diversity. 2021; ():1-16.
Chicago/Turabian StyleChonny Herrera-Acevedo; Camilo Perdomo-Madrigal; Kenyi Herrera-Acevedo; Ericsson Coy-Barrera; Luciana Scotti; Marcus Tullius Scotti. 2021. "Machine learning models to select potential inhibitors of acetylcholinesterase activity from SistematX: a natural products database." Molecular Diversity , no. : 1-16.
The current treatments against Leishmania parasites present high toxicity and multiple side effects, which makes the control and elimination of leishmaniasis challenging. Natural products constitute an interesting and diverse chemical space for the identification of new antileishmanial drugs. To identify new drug options, an in-house database of 360 kauranes (tetracyclic diterpenes) was generated, and a combined ligand- and structure-based virtual screening (VS) approach was performed to select potential inhibitors of Leishmania major (Lm) pteridine reductase I (PTR1). The best-ranked kauranes were employed to verify the validity of the VS approach through LmPTR1 enzyme inhibition assay. The half-maximal inhibitory concentration (IC50) values of selected bioactive compounds were examined using the random forest (RF) model (i.e., 2β-hydroxy-menth-6-en-5β-yl ent-kaurenoate (135) and 3α-cinnamoyloxy-ent-kaur-16-en-19-oic acid (302)) were below 10 μM. A compound similar to 302, 3α-p-coumaroyloxy-ent-kaur-16-en-19-oic acid (302a), was also synthesized and showed the highest activity against LmPTR1. Finally, molecular docking calculations and molecular dynamics simulations were performed for the VS-selected, most-active kauranes within the active sites of PTR1 hybrid models, generated from three Leishmania species that are known to cause cutaneous leishmaniasis in the new world (i.e., L. braziliensis, L. panamensis, and L. amazonensis) to explore the targeting potential of these kauranes to other species-dependent variants of this enzyme.
Chonny Herrera-Acevedo; Areli Flores-Gaspar; Luciana Scotti; Francisco Mendonça-Junior; Marcus Scotti; Ericsson Coy-Barrera. Identification of Kaurane-Type Diterpenes as Inhibitors of Leishmania Pteridine Reductase I. Molecules 2021, 26, 3076 .
AMA StyleChonny Herrera-Acevedo, Areli Flores-Gaspar, Luciana Scotti, Francisco Mendonça-Junior, Marcus Scotti, Ericsson Coy-Barrera. Identification of Kaurane-Type Diterpenes as Inhibitors of Leishmania Pteridine Reductase I. Molecules. 2021; 26 (11):3076.
Chicago/Turabian StyleChonny Herrera-Acevedo; Areli Flores-Gaspar; Luciana Scotti; Francisco Mendonça-Junior; Marcus Scotti; Ericsson Coy-Barrera. 2021. "Identification of Kaurane-Type Diterpenes as Inhibitors of Leishmania Pteridine Reductase I." Molecules 26, no. 11: 3076.
Colombia is the main producer of cape gooseberry (Physalis peruviana L.), a plant known for its various consumption practices and medicinal properties. This plant is generally grown in eroded soils and is considered moderately tolerant to unfavorable conditions, such as nutrient-poor soils or high salt concentrations. Most studies conducted on this plant focus on fruit production and composition because it is the target product, but a small number of studies have been conducted to describe the effect of abiotic stress, e.g., salt stress, on growth and biochemical responses. In order to better understand the mechanism of inherent tolerance of this plant facing salt stress, the present study was conducted to determine the metabolic and growth differences of P. peruviana plants at three different BBCH-based growth substages, varying salt conditions. Hence, plants were independently treated with two NaCl solutions, and growth parameters and LC-ESI-MS-derived semi-quantitative levels of metabolites were then measured and compared between salt treatments per growth substage. A 90 mM NaCl treatment caused the greatest effect on plants, provoking low growth and particular metabolite variations. The treatment discrimination-driving feature classification suggested that glycosylated flavonols increased under 30 mM NaCl at 209 substages, withanolides decreased under 90 mM NaCl at 603 and 703 substages, and up-regulation of a free flavonol at all selected stages can be considered a salt stress response. Findings locate such response into a metabolic context and afford some insights into the plant response associated with antioxidant compound up-regulation.
Daissy Monroy-Velandia; Ericsson Coy-Barrera. Effect of Salt Stress on Growth and Metabolite Profiles of Cape Gooseberry (Physalis peruviana L.) along Three Growth Stages. Molecules 2021, 26, 2756 .
AMA StyleDaissy Monroy-Velandia, Ericsson Coy-Barrera. Effect of Salt Stress on Growth and Metabolite Profiles of Cape Gooseberry (Physalis peruviana L.) along Three Growth Stages. Molecules. 2021; 26 (9):2756.
Chicago/Turabian StyleDaissy Monroy-Velandia; Ericsson Coy-Barrera. 2021. "Effect of Salt Stress on Growth and Metabolite Profiles of Cape Gooseberry (Physalis peruviana L.) along Three Growth Stages." Molecules 26, no. 9: 2756.
Background The aim of this study was to measure the dental pulp inflammatory response through neuropeptides (SP and CGRP) as a response to occlusal trauma, orthodontic movements and a combination of both, as well as the angiogenic defense mechanism through VEGF expression, which could be the initial step to mineralized tissue formation. Methods Forty human dental pulp samples were collected from healthy first premolars with extraction indicated due to orthodontic reasons from a sample of 20 patients. Patients were divided into four groups with 10 premolars each (1 mandibular and 1 maxillary premolar from each patient): healthy pulp control group, occlusal trauma group, moderate orthodontic forces group; and occlusal trauma plus moderate orthodontic forces group. Stimuli were applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and VEGF were measured by radioimmunoassay. The Kruskal–Wallis test was performed to assess significant differences among groups and Mann–Whitney’s U post hoc pairwise comparisons were also performed. Results The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic forces group, followed by the moderate orthodontic forces, the occlusal trauma and the control groups, with statistically significant differences between all groups for each of the 3 peptides analyzed (Kruskal–Wallis p < 0.001). All possible pairwise post-hoc comparisons were also significant for each peptide analyzed (Mann–Whitney’s U p < 0.001). Conclusion SP, CGRP, and VEGF expressions significantly increase in human dental pulps when stimulated by occlusal trauma combined with moderate orthodontic forces, as compared with these two stimuli applied independently. Name of the registry: Importance of Neurogenic Inflammation in the Angiogenic Response of the Dental Pulp as a Defensive Response. Trial registration number: NCT03804034. Date of registration: 01/15/2019 Retrospectively registered. URL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT03804034?term=NCT03804034&draw=2&rank=1.
Javier Caviedes-Bucheli; Luis Fernando Lopez-Moncayo; Hernan Dario Muñoz-Alvear; Jose Francisco Gomez-Sosa; Luis Eduardo Diaz-Barrera; Hernando Curtidor; Hugo Roberto Munoz. Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli. BMC Oral Health 2021, 21, 1 -8.
AMA StyleJavier Caviedes-Bucheli, Luis Fernando Lopez-Moncayo, Hernan Dario Muñoz-Alvear, Jose Francisco Gomez-Sosa, Luis Eduardo Diaz-Barrera, Hernando Curtidor, Hugo Roberto Munoz. Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli. BMC Oral Health. 2021; 21 (1):1-8.
Chicago/Turabian StyleJavier Caviedes-Bucheli; Luis Fernando Lopez-Moncayo; Hernan Dario Muñoz-Alvear; Jose Francisco Gomez-Sosa; Luis Eduardo Diaz-Barrera; Hernando Curtidor; Hugo Roberto Munoz. 2021. "Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli." BMC Oral Health 21, no. 1: 1-8.
Leishmaniasis is a parasitic morbid/fatal disease caused by Leishmania protozoa. Twelve million people worldwide are appraised to be currently infected, including ca. two million infections each year, and 350 million people in 88 countries are at risk of becoming infected. In Colombia, cutaneous leishmaniasis (CL) is a public health problem in some tropical areas. Therapeutics is based on traditional antileishmanial drugs, but this practice has several drawbacks for patients. Thus, the search for new antileishmanial agents is a serious need, but the lack of adequately funded research programs on drug discovery has hampered its progress. Some Colombian researchers have conducted different research projects focused on the assessment of the antileishmanial activity of naturally occurring and synthetic compounds against promastigotes and/or amastigotes. Results of such studies have separately demonstrated important hits and reasonable potential, but a holistic view of them is lacking. Hence, we present the outcome from a systematic review of the literature (under PRISMA guidelines) on those Colombian studies investigating antileishmanials during the last thirty-two years. In order to combine the general efforts aiming at finding a lead against Leishmania panamensis (one of the most studied and incident parasites in Colombia causing CL) and to recognize structural features of representative compounds, fingerprint-based analyses using conventional machine learning algorithms and clustering methods are shown. Abstraction from such a meta-description led to describe some function-determining molecular features and simplify the clustering of plausible isofunctional hits. This systematic review indicated that the Colombian efforts for the antileishmanials discovery are increasingly intensified, though improvements in the followed pathways must be definitively pursued. In this context, a brief discussion about scope, strengths and limitations of such advances and relationships is addressed.
Jeysson Sánchez-Suárez; Freddy A. Bernal; Ericsson Coy-Barrera. Colombian Contributions Fighting Leishmaniasis: A Systematic Review on Antileishmanials Combined with Chemoinformatics Analysis. Molecules 2020, 25, 5704 .
AMA StyleJeysson Sánchez-Suárez, Freddy A. Bernal, Ericsson Coy-Barrera. Colombian Contributions Fighting Leishmaniasis: A Systematic Review on Antileishmanials Combined with Chemoinformatics Analysis. Molecules. 2020; 25 (23):5704.
Chicago/Turabian StyleJeysson Sánchez-Suárez; Freddy A. Bernal; Ericsson Coy-Barrera. 2020. "Colombian Contributions Fighting Leishmaniasis: A Systematic Review on Antileishmanials Combined with Chemoinformatics Analysis." Molecules 25, no. 23: 5704.
Water contamination by mercury and chromium has a direct effect in human health. A promising technology to remove heavy metals by membrane filtration is the use of hybrid membranes produced with whey protein fibrils (WPF) and activated carbon (AC). In this study, the best conditions to produce WPF by heat treatment were determined to maximize the removal of mercury and chromium from water using a central composed design. The results indicated that the best conditions to prepare WPF were 74 °C, 7 h and 3.8% of whey protein with adsorption capacities of 25 and 18 mg/g and removal efficiencies of 81 and 57% for mercury and chromium, respectively. WPF and AC were used to prepare a hybrid membrane that was characterized using transmission electron microscopy, atomic force microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy and Brunauer–Emmett–Teller surface area measurements. Batch filtration experiments were performed with the hybrid membrane for chromium and mercury removal at 25, 50 and 100 mg/L to determine its adsorption capacities. A high performance of the hybrid membrane was demonstrated removing efficiently mercury and chromium from water, thus supporting more than ten filtration cycles.
Laura Ramírez-Rodríguez; Luis Díaz Barrera; María Quintanilla-Carvajal; Didilia Mendoza-Castillo; Adrián Bonilla-Petriciolet; Carlos Jiménez-Junca. Preparation of a Hybrid Membrane from Whey Protein Fibrils and Activated Carbon to Remove Mercury and Chromium from Water. Membranes 2020, 10, 386 .
AMA StyleLaura Ramírez-Rodríguez, Luis Díaz Barrera, María Quintanilla-Carvajal, Didilia Mendoza-Castillo, Adrián Bonilla-Petriciolet, Carlos Jiménez-Junca. Preparation of a Hybrid Membrane from Whey Protein Fibrils and Activated Carbon to Remove Mercury and Chromium from Water. Membranes. 2020; 10 (12):386.
Chicago/Turabian StyleLaura Ramírez-Rodríguez; Luis Díaz Barrera; María Quintanilla-Carvajal; Didilia Mendoza-Castillo; Adrián Bonilla-Petriciolet; Carlos Jiménez-Junca. 2020. "Preparation of a Hybrid Membrane from Whey Protein Fibrils and Activated Carbon to Remove Mercury and Chromium from Water." Membranes 10, no. 12: 386.
In this paper, we describe the solvent-free three-component synthesis of 2,4,5-trisubstituted-1H-pyrrol-3-ol-type compounds from L-tryptophan. The first step of the synthetic methodology involved the esterification of L-tryptophan in excellent yields (93–98%). Equimolar mixtures of alkyl 2-aminoesters, 1,3-dicarbonyl compounds, and potassium hydroxide (0.1 eq.) were heated under solvent-free conditions. The title compounds were obtained in moderate to good yields (45%–81%). Density functional theory using “Becke, 3-parameter, Lee–Yang–Parr” correlational functional (DFT-B3LYP) calculations were performed to understand the molecular stability of the synthesized compounds and the tautomeric equilibrium from 3H-pyrrol-3-one type intermediates to 1H-pyrrol-3-ol type aromatized rings.
Diego Quiroga; Lili Dahiana Becerra; Ericsson Coy-Barrera. Solvent Free Three-Component Synthesis of 2,4,5-trisubstituted-1H-pyrrol-3-ol-type Compounds from L-tryptophan: DFT-B3LYP Calculations for the Reaction Mechanism and 3H-pyrrol-3-one↔1H-pyrrol-3-ol Tautomeric Equilibrium. Molecules 2020, 25, 4402 .
AMA StyleDiego Quiroga, Lili Dahiana Becerra, Ericsson Coy-Barrera. Solvent Free Three-Component Synthesis of 2,4,5-trisubstituted-1H-pyrrol-3-ol-type Compounds from L-tryptophan: DFT-B3LYP Calculations for the Reaction Mechanism and 3H-pyrrol-3-one↔1H-pyrrol-3-ol Tautomeric Equilibrium. Molecules. 2020; 25 (19):4402.
Chicago/Turabian StyleDiego Quiroga; Lili Dahiana Becerra; Ericsson Coy-Barrera. 2020. "Solvent Free Three-Component Synthesis of 2,4,5-trisubstituted-1H-pyrrol-3-ol-type Compounds from L-tryptophan: DFT-B3LYP Calculations for the Reaction Mechanism and 3H-pyrrol-3-one↔1H-pyrrol-3-ol Tautomeric Equilibrium." Molecules 25, no. 19: 4402.
Leishmaniasis refers to a complex of diseases, caused by the intracellular parasitic protozoans belonging to the genus Leishmania. Among the three types of disease manifestations, the most severe type is visceral leishmaniasis, which is caused by Leishmania donovani, and is diagnosed in more than 20,000 cases annually, worldwide. Because the current therapeutic options for disease treatment are associated with several limitations, the identification of new potential leads/drugs remains necessary. In this study, a combined approach was used, based on two different virtual screening (VS) methods, which were designed to select promising antileishmanial agents from among the entire sesquiterpene lactone (SL) dataset registered in SistematX, a web interface for managing a secondary metabolite database that is accessible by multiple platforms on the Internet. Thus, a ChEMBL dataset, including 3159 and 1569 structures that were previously tested against L. donovani amastigotes and promastigotes in vitro, respectively, was used to develop two random forest models, which performed with greater than 74% accuracy in both the cross-validation and test sets. Subsequently, a ligand-based VS assay was performed against the 1306 SistematX-registered SLs. In parallel, the crystal structures of three L. donovani target proteins, N-myristoyltransferase, ornithine decarboxylase, and mitogen-activated protein kinase 3, and a homology model of pteridine reductase 1 were used to perform a structure-based VS, using molecular docking, of the entire SistematX SL dataset. The consensus analysis of these two VS approaches resulted in the normalization of probability scores and identified 13 promising, enzyme-targeting, antileishmanial SLs from SistematX that may act against L. donovani. A combined approach based on two different virtual screening methods (structure-based and ligand-based) was performed using an in-house dataset composed of 1306 sesquiterpene lactones to identify potential antileishmanial (Leishmania donovani) structures.
Chonny Herrera-Acevedo; Mayara Dos Santos Maia; Élida Batista Vieira Sousa Cavalcanti; Ericsson Coy-Barrera; Luciana Scotti; Marcus Tullius Scotti. Selection of antileishmanial sesquiterpene lactones from SistematX database using a combined ligand-/structure-based virtual screening approach. Molecular Diversity 2020, 1 -17.
AMA StyleChonny Herrera-Acevedo, Mayara Dos Santos Maia, Élida Batista Vieira Sousa Cavalcanti, Ericsson Coy-Barrera, Luciana Scotti, Marcus Tullius Scotti. Selection of antileishmanial sesquiterpene lactones from SistematX database using a combined ligand-/structure-based virtual screening approach. Molecular Diversity. 2020; ():1-17.
Chicago/Turabian StyleChonny Herrera-Acevedo; Mayara Dos Santos Maia; Élida Batista Vieira Sousa Cavalcanti; Ericsson Coy-Barrera; Luciana Scotti; Marcus Tullius Scotti. 2020. "Selection of antileishmanial sesquiterpene lactones from SistematX database using a combined ligand-/structure-based virtual screening approach." Molecular Diversity , no. : 1-17.
The effects of an adequate supply of vitamin A and iron, in comparison with diets low or absent in vitamin A and low in iron, on the mRNA expression of some biomarkers of iron homeostasis as hepcidin ( Hamp), transferrin receptor-1 ( Tfrc), iron regulatory protein-2 ( Ireb2) and ferritin ( Fth1) in rats were investigated. 35 male Wistar rats were randomly divided into 5 dietary groups: control, sufficient in iron and insufficient in vitamin A (FesvAi), sufficient in iron and depleted in vitamin A (FesvAd), insufficient in iron and sufficient in vitamin A (FeivAs) and insufficient in both iron and vitamin A (FeivAi). After 6 weeks rats showed no significant effects of variations in vitamin A on the expression of Hamp relative to the control group (FesvAi: 1.37-fold; FesvAd: 1.22-fold); however, iron deficiency showed significant reduction on it relative to the control group (FeivAs: 71.4-fold, P = 0.0004; FeivAi: 16.1-fold, P = 0.0008). Vitamin A deficiency (FesvAd) affects expression of Fth1 independent of low dietary iron in spleen (0.29-fold, P = 0.002) and duodenum (5.15-fold, P = 0.02). Variations of dietary iron and vitamin A showed significant effects relative to the control group for expression of Tfrc in spleen (FesvAd: 0.18-fold, P = 0.01; FeivAs: 0.24-fold, P < 0.0001; FeivAi: 0.42-fold, P = 0.014), Ireb2 in spleen (FeivAs: 3.7-fold, P < 0.0001; FeivAi: 2.9-fold, P < 0.0001) and Ireb2 in duodenum (FeivAs: 2.68-fold, P = 0.012; FeivAi: 2.60-fold, P = 0.014). These results show that vitamin A and iron must be supplied together to regulate some of the main biomarkers of iron metabolism as a strategy to reduce prevalence of iron deficiency anemia.
Mauricio Restrepo-Gallego; Luis E. Díaz. Vitamin A does not influence mRNA expression of hormone hepcidin but other biomarkers of iron homeostasis in young male Wistar rats. International Journal for Vitamin and Nutrition Research 2020, 1 -8.
AMA StyleMauricio Restrepo-Gallego, Luis E. Díaz. Vitamin A does not influence mRNA expression of hormone hepcidin but other biomarkers of iron homeostasis in young male Wistar rats. International Journal for Vitamin and Nutrition Research. 2020; ():1-8.
Chicago/Turabian StyleMauricio Restrepo-Gallego; Luis E. Díaz. 2020. "Vitamin A does not influence mRNA expression of hormone hepcidin but other biomarkers of iron homeostasis in young male Wistar rats." International Journal for Vitamin and Nutrition Research , no. : 1-8.
Sun overexposure is associated with the development of diseases that primarily affect the skin, which can lead to skin cancer. Among the main measures of photoprotection is the use of sunscreens. However, there is currently concern about the reported harmful effects to both humans and the environment due to several of the sunscreen ingredients available on the market. For this reason, the search for and development of new agents with photoprotective properties is required. In searching for these metabolites, researchers have turned their attention to microbial sources, especially the microbiota in unusual hostile environments. Among the diverse microorganisms available in nature, Actinobacteria and specifically Streptomyces, have been shown to be a source of metabolites with various biological activities of interest, such as antimicrobial, antitumor and immunomodulator activities. Herein, we present the results of a systematic review of the literature in which Streptomyces isolates were studied as a source of compounds with photoprotective properties. A meta-analysis of the structure-property and structure-activity relationships of those metabolites identified in the qualitative analysis phase was also carried out. These findings indicate that Streptomyces are a source of metabolites with potential applications in the development of new, safe and more eco-friendly sunscreens.
Jeysson Sánchez-Suárez; Luis Eduardo Díaz Barrera; Luisa Villamil; Luis Díaz. Streptomyces-Derived Metabolites with Potential Photoprotective Properties—A Systematic Literature Review and Meta-Analysis on the Reported Chemodiversity. Molecules 2020, 25, 3221 .
AMA StyleJeysson Sánchez-Suárez, Luis Eduardo Díaz Barrera, Luisa Villamil, Luis Díaz. Streptomyces-Derived Metabolites with Potential Photoprotective Properties—A Systematic Literature Review and Meta-Analysis on the Reported Chemodiversity. Molecules. 2020; 25 (14):3221.
Chicago/Turabian StyleJeysson Sánchez-Suárez; Luis Eduardo Díaz Barrera; Luisa Villamil; Luis Díaz. 2020. "Streptomyces-Derived Metabolites with Potential Photoprotective Properties—A Systematic Literature Review and Meta-Analysis on the Reported Chemodiversity." Molecules 25, no. 14: 3221.
Iron deficiency is the leading cause of anemia, a significant global public health problem. Different methods exist for assessing iron nutritional status, including laboratory tests that focus on storage, transportation, and iron functional compartment parameters. Classical markers such as bone marrow, serum iron, ferritin, hemoglobin, erythrocyte parameters, transferrin, transferrin receptors, and zinc protoporphyrin are discussed in this review. Additional parameters calculated from these indicators, including transferrin saturation, ferritin index and Thomas plot, and some emergent parameters such as hepcidin, erythroferrone, and low hemoglobin density are also discussed. There is no a single indicator for assessing iron nutritional status. Therefore, the use of more than one indicator may be the best practice to obtain the correct diagnosis, also considering the influence of inflammation/infection on many of these indicators. The constant validation of the current parameters, the improvement of assessment methods, and the identification of new indicators will be the key to refine the assessment of iron nutritional status and the right choice of treatment for its improvement.
Mauricio Restrepo-Gallego; Luis E. Díaz; Patrícia H.C Rondó. Classic and emergent indicators for the assessment of human iron status. Critical Reviews in Food Science and Nutrition 2020, 1 -14.
AMA StyleMauricio Restrepo-Gallego, Luis E. Díaz, Patrícia H.C Rondó. Classic and emergent indicators for the assessment of human iron status. Critical Reviews in Food Science and Nutrition. 2020; ():1-14.
Chicago/Turabian StyleMauricio Restrepo-Gallego; Luis E. Díaz; Patrícia H.C Rondó. 2020. "Classic and emergent indicators for the assessment of human iron status." Critical Reviews in Food Science and Nutrition , no. : 1-14.
Polyurethanes (PUs) from Polyethylene glycol (PEG) and polycaprolactone diol (PCL) and a crosslinker, Pentaerythritol (PE), were synthetized with isophorone diisocyanate (IPDI). In this study, we investigated the effect of polyol and crosslinker composition on phase separation and thermo-mechanical properties. The properties were studied through dynamic mechanical analysis, X-ray scattering, atomic force microscopy (AFM), and thermogravimetric analysis (TGA). The results showed changes in PUs properties, microphase structure, and separation due to the composition of polyol/crosslinker blend. So, the largest concentration of PE produced multimodal loss factor patterns, indicating segment segregation while PUs with a PEG/PCL = 1 displayed a monomodal loss factor pattern, indicating a homogeneously distributed microphase separation. Additionally, the increase of the PEG concentration enhanced the damping capacity. On the other hand, agglomeration and thread-like structures of hard segments (HS) were observed through AFM. Finally, the thermal behavior of PUs was affected by chemical composition. Lower concentration of PE reduced the crosslinking; hence, the temperature with the maximum degradation rate.
Said Arévalo-Alquichire; Maria Morales-Gonzalez; Kelly Navas-Gómez; Luis E. Diaz; José A. Gómez-Tejedor; María-Antonia Serrano; Manuel F. Valero. Influence of Polyol/Crosslinker Blend Composition on Phase Separation and Thermo-Mechanical Properties of Polyurethane Thin Films. Polymers 2020, 12, 666 .
AMA StyleSaid Arévalo-Alquichire, Maria Morales-Gonzalez, Kelly Navas-Gómez, Luis E. Diaz, José A. Gómez-Tejedor, María-Antonia Serrano, Manuel F. Valero. Influence of Polyol/Crosslinker Blend Composition on Phase Separation and Thermo-Mechanical Properties of Polyurethane Thin Films. Polymers. 2020; 12 (3):666.
Chicago/Turabian StyleSaid Arévalo-Alquichire; Maria Morales-Gonzalez; Kelly Navas-Gómez; Luis E. Diaz; José A. Gómez-Tejedor; María-Antonia Serrano; Manuel F. Valero. 2020. "Influence of Polyol/Crosslinker Blend Composition on Phase Separation and Thermo-Mechanical Properties of Polyurethane Thin Films." Polymers 12, no. 3: 666.
Gelatin-based nanofibres (NF) incorporating a high-oleic palm oil (HOPO) nanoemulsion or macroemulsion were electrospun. An I-optimal response surface design was used for studying the effect of gelatin mass fraction, tip-collector distance and emulsion type (factors) on fibre diameter (Df). The NF had a Df ranging from 136.9 to 240.8 nm and no beads; synergic effects between factors were observed as significant over Df. Furthermore, topography, roughness, oil distribution, peroxide value (PV), antioxidant activity (AOX), thermal stability and crystallinity of two optimised NF were analysed; smooth and slightly rugose NF were observed by atomic force microscopy (AFM) and heterogeneously distributed oil by confocal laser scanning microscopy (CLSM). Microfluidisation and electrospinning had low PV, high encapsulation efficiency (>94 %) and high AA values, i.e., efficient encapsulation processes. NF thermal stability was affected by emulsion type; a more amorphous structure tended to give higher droplet size inside fibres. Despite Df was not affected by emulsion droplet size, NF’s physico-chemical properties showed changes.
Leidy Ricaurte; Patricio Román Santagapita; Luis Eduardo Díaz; Maria Ximena Quintanilla-Carvajal. Edible gelatin-based nanofibres loaded with oil encapsulating high-oleic palm oil emulsions. Colloids and Surfaces A: Physicochemical and Engineering Aspects 2020, 595, 124673 .
AMA StyleLeidy Ricaurte, Patricio Román Santagapita, Luis Eduardo Díaz, Maria Ximena Quintanilla-Carvajal. Edible gelatin-based nanofibres loaded with oil encapsulating high-oleic palm oil emulsions. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2020; 595 ():124673.
Chicago/Turabian StyleLeidy Ricaurte; Patricio Román Santagapita; Luis Eduardo Díaz; Maria Ximena Quintanilla-Carvajal. 2020. "Edible gelatin-based nanofibres loaded with oil encapsulating high-oleic palm oil emulsions." Colloids and Surfaces A: Physicochemical and Engineering Aspects 595, no. : 124673.
Prostaglandin A2-AcMe (1) and Prostaglandin A2 (2) were isolated from the octocoral Plexaura homomalla and three semisynthetic derivatives (3–5) were then obtained using a reduction protocol. All compounds were identified through one- and two-dimensional (1D and 2D) nuclear magnetic resonance (NMR) experiments. Additionally, evaluation of in vitro cytotoxic activity against the breast (MDA-MB-213) and lung (A549) cancer cell lines, in combination with enzymatic activity and molecular docking studies with the enzymes p38α-kinase, Src-kinase, and topoisomerase IIα, were carried out for compounds 1–5 in order to explore their potential as inhibitors of cancer-related molecular targets. Results showed that prostaglandin A2 (2) was the most potent compound with an IC50 of 16.46 and 25.20 μg/mL against MDA-MB-213 and A549 cell lines, respectively. In addition, this compound also inhibited p38α-kinase in 49% and Src-kinase in 59% at 2.5 μM, whereas topoisomerase IIα was inhibited in 64% at 10 μM. Enzymatic activity was found to be consistent with molecular docking simulations, since compound 2 also showed the lowest docking scores against the topoisomerase IIα and Src-kinase (−8.7 and −8.9 kcal/mol, respectively). Thus, molecular docking led to establish some insights into the predicted binding modes. Results suggest that prostaglandin 2 can be considered as a potential lead for development inhibitors against some enzymes present in cancer processes.
Diana Ximena Hurtado; Fabio A. Castellanos; Ericsson Coy-Barrera; Edisson Tello. Prostaglandins Isolated from the Octocoral Plexaura homomalla: In Silico and In Vitro Studies Against Different Enzymes of Cancer. Marine Drugs 2020, 18, 141 .
AMA StyleDiana Ximena Hurtado, Fabio A. Castellanos, Ericsson Coy-Barrera, Edisson Tello. Prostaglandins Isolated from the Octocoral Plexaura homomalla: In Silico and In Vitro Studies Against Different Enzymes of Cancer. Marine Drugs. 2020; 18 (3):141.
Chicago/Turabian StyleDiana Ximena Hurtado; Fabio A. Castellanos; Ericsson Coy-Barrera; Edisson Tello. 2020. "Prostaglandins Isolated from the Octocoral Plexaura homomalla: In Silico and In Vitro Studies Against Different Enzymes of Cancer." Marine Drugs 18, no. 3: 141.
Exploration of the effect of soil bacteria on growth and metabolism of beneficial root endophytic fungi is relevant to promote favorable associations between microorganisms of the plant rhizosphere. Hence, the interaction between the plant-growth-promoting fungus Piriformospora indica and different soil bacteria was investigated. The parameters studied were fungal growth and its amino acid composition during the interaction. Fungus and bacteria were confronted in dual cultures in Petri dishes, either through agar or separated by a Perspex wall that only allowed the bacterial volatiles to be effective. Fungal growth was stimulated by Azotobacter chroococcum, whereas Streptomyces anulatus AcH 1003 inhibited it and Streptomyces sp. Nov AcH 505 had no effect. To analyze amino acid concentration data, targeted metabolomics was implemented under supervised analysis according to fungal-bacteria interaction and time. Orthogonal partial least squares-discriminant analysis (OPLS-DA) model clearly discriminated P. indica–A. chroococcum and P. indica–S. anulatus interactions, according to the respective score plot in comparison to the control. The most observable responses were in the glutamine and alanine size groups: While Streptomyces AcH 1003 increased the amount of glutamine, A. chroococcum decreased it. The fungal growth and the increase of alanine content might be associated with the assimilation of nitrogen in the presence of glucose as a carbon source. The N-fixing bacterium A. chroococcum should stimulate fungal amino acid metabolism via glutamine synthetase-glutamate synthase (GS-GOGAT). The data pointed to a stimulated glycolytic activity in the fungus observed by the accumulation of alanine, possibly via alanine aminotransferase. The responses toward the growth-inhibiting Streptomyces AcH 1003 suggest an (oxidative) stress response of the fungus.
Jorge A. Leyva-Rojas; Ericsson Coy-Barrera; Rüdiger Hampp. Interaction with Soil Bacteria Affects the Growth and Amino Acid Content of Piriformospora indica. Molecules 2020, 25, 572 .
AMA StyleJorge A. Leyva-Rojas, Ericsson Coy-Barrera, Rüdiger Hampp. Interaction with Soil Bacteria Affects the Growth and Amino Acid Content of Piriformospora indica. Molecules. 2020; 25 (3):572.
Chicago/Turabian StyleJorge A. Leyva-Rojas; Ericsson Coy-Barrera; Rüdiger Hampp. 2020. "Interaction with Soil Bacteria Affects the Growth and Amino Acid Content of Piriformospora indica." Molecules 25, no. 3: 572.
2-arylbenzofuran-containing compounds are chemical entities that can be naturally produced by several organisms. A wide-range of activities is described for several compounds of this kind and they are, therefore, valuable moieties for a lead finding from nature. Although there are in-vitro data about the activity of 2-arylbenzofuran-related compounds against cyclooxygenase (COX) enzymes, the molecular level of these COX-inhibiting constituents had not been deeply explored. Thus, 58 2-arylbenzofurans were initially screened through molecular docking within the active site of nine COX-2 crystal structures. The resulting docking scores were statistically analyzed and good reproducibility and convergence were found to discriminate the best-docked compounds. Discriminated compounds exhibited the best performance in molecular dynamics simulations as well as the most-favorable binding energies and the lowest in-vitro IC50 values for COX-2 inhibition. A three-dimensional quantitative activity-structure relationship (3D-QSAR) was also demonstrated, which showed some crucial structural requirements for enhanced enzyme inhibition. Therefore, four hits are proposed as lead structures for the development of COX-2 inhibitors based on 2-arylbenzofurans in further studies.
Ericsson Coy-Barrera. Discrimination of Naturally-Occurring 2-Arylbenzofurans as Cyclooxygenase-2 Inhibitors: Insights into the Binding Mode and Enzymatic Inhibitory Activity. Biomolecules 2020, 10, 176 .
AMA StyleEricsson Coy-Barrera. Discrimination of Naturally-Occurring 2-Arylbenzofurans as Cyclooxygenase-2 Inhibitors: Insights into the Binding Mode and Enzymatic Inhibitory Activity. Biomolecules. 2020; 10 (2):176.
Chicago/Turabian StyleEricsson Coy-Barrera. 2020. "Discrimination of Naturally-Occurring 2-Arylbenzofurans as Cyclooxygenase-2 Inhibitors: Insights into the Binding Mode and Enzymatic Inhibitory Activity." Biomolecules 10, no. 2: 176.
Plants of the genus Genista has gained interest due to its high content of alkaloids and flavonoids, especially isoflavones, because of their phytoestrogenic activity. Genista monspessulana (Fabaceae) is endemic of the Mediterranean area, known as “Cape broom”, but it is also considered as an invasive plant in some places into neotropical area. This plant is also known for its high content of secondary products with multi-pharmacological activity, e.g. antioxidant and phytoestrogenic, which has attracted attention for their potential use in preventive medicine. Thus, the present study was carried out to determine the variation on the DPPH• scavenging capacity and isoflavone content of in vitro seedlings of Cape broom after the stimulus of phytohormones such as indole-3-acetic acid (IAA) and kinetin (KIN), as a biochemical response for future biotechnological studies. Results indicated that the highest germination percentage was achieved in seeds separately exposed to IAA (at 2.5 µM under darkness and 7.5 µM under light) and KIN (at 0.25 µM under light and 0.75 µM under darkness. DPPH• free radical scavenging capacity, total phenolic and total flavonoid contents for in vitro seedlings of G. monspessulana treated with phytohormones were generally higher to that of intact plant. Additionally, in vitro seedlings showed enhanced concentrations of glycosylated isoflavones in the presence of IAA under light exposition. Results indicated that phytohormones (IAA or KIN) affected total phenolic/flavonoid contents, DPPH• scavenging capacity and isoflavone concentrations on in vitro seedlings, which constitutes a biotechnological opportunity to expand the exploitation of isoflavones from G. monspessulana.
A. Meza; P. Rojas; W. Cely-Veloza; C. Guerrero-Perilla; E. Coy-Barrera. Variation of isoflavone content and DPPH• scavenging capacity of phytohormone-treated seedlings after in vitro germination of cape broom (Genista monspessulana). South African Journal of Botany 2019, 130, 64 -74.
AMA StyleA. Meza, P. Rojas, W. Cely-Veloza, C. Guerrero-Perilla, E. Coy-Barrera. Variation of isoflavone content and DPPH• scavenging capacity of phytohormone-treated seedlings after in vitro germination of cape broom (Genista monspessulana). South African Journal of Botany. 2019; 130 ():64-74.
Chicago/Turabian StyleA. Meza; P. Rojas; W. Cely-Veloza; C. Guerrero-Perilla; E. Coy-Barrera. 2019. "Variation of isoflavone content and DPPH• scavenging capacity of phytohormone-treated seedlings after in vitro germination of cape broom (Genista monspessulana)." South African Journal of Botany 130, no. : 64-74.
There is a continuous search for more reliable and effective alternatives to control phytopathogens through different strategies. In this context, indole-containing phytoalexins are stimuli-induced compounds implicated in plant defense against plant pathogens. However, phytoalexins’ efficacy have been limited by fungal detoxifying mechanisms, thus, the research on bioisosteres-based analogs can be a friendly alternative regarding the control of Fusarium phytopathogens, but there are currently few studies on it. Thus, as part of our research on antifungal agents, a set of 21 synthetic indole-containing phytoalexin analogs were evaluated as inhibitors against the phyopathogen Fusarium oxysporum. Results indicated that analogs of the N,N-dialkylthiourea, N,S-dialkyldithiocarbamate and substituted-1,3-thiazolidin-5-one groups exhibited the best docking scores and interaction profiles within the active site of Fusarium spp. enzymes. Vina scores exhibited correlation with experimental mycelial growth inhibition using supervised statistics, and this antifungal dataset correlated with molecular interaction fields after CoMFA. Compound 24 (tert-butyl (((3-oxo-1,3-diphenylpropyl)thio)carbonothioyl)-l-tryptophanate), a very active analog against F. oxysporum, exhibited the best interaction with lanosterol 14α-demethylase according to molecular docking, molecular dynamics and molecular mechanic/poisson-boltzmann surface area (MM/PBSA) binding energy performance. After data analyses, information on mycelial growth inhibitors, structural requirements and putative enzyme targets may be used in further antifungal development based on phytoalexin analogs for controlling phytopathogens.
Andrea Angarita-Rodríguez; Diego Quiroga; Ericsson Coy-Barrera. Indole-Containing Phytoalexin-Based Bioisosteres as Antifungals: In Vitro and In Silico Evaluation against Fusarium oxysporum. Molecules 2019, 25, 45 .
AMA StyleAndrea Angarita-Rodríguez, Diego Quiroga, Ericsson Coy-Barrera. Indole-Containing Phytoalexin-Based Bioisosteres as Antifungals: In Vitro and In Silico Evaluation against Fusarium oxysporum. Molecules. 2019; 25 (1):45.
Chicago/Turabian StyleAndrea Angarita-Rodríguez; Diego Quiroga; Ericsson Coy-Barrera. 2019. "Indole-Containing Phytoalexin-Based Bioisosteres as Antifungals: In Vitro and In Silico Evaluation against Fusarium oxysporum." Molecules 25, no. 1: 45.