Medicinal-aromatic plants (MAPs) are important sources for the development of new valuable products of interest to human and animal health, and are also used as ornamentals for the horticulture industry. However, the increased global demand and the uncontrolled exploitation of these plants constitute a threat to their sustainability. To date, few scientific investigations have focused on MAPs valorization and their domestication. The purpose of this study was to evaluate for the first time the medicinal-cosmetic potential of 399 local endemic Mediterranean plants confined to Crete (223 taxa), the Mediterranean coast-Rif of Morocco (94), and Tunisia (82). The new methodological scheme was developed by experts through three multidisciplinary co-creative workshops and was adjusted by end-users to point-scoring of nine attributes evaluating the potential of the targeted neglected and underutilized plants (NUPs) in the medicinal-cosmetic sector. The results were demonstrated as percentage of the maximum possible score. These assessments were further linked and discussed with respect to feasibility and readiness timescale evaluations for sustainable exploitation of the focal NUPs. A great diversity of local endemic NUPs (30 taxa, 11 families) were associated with interesting medicinal-cosmetic properties (>35% up to 94.44%). Among them, 8 taxa showed the highest medicinal-cosmetic potential (>55% of maximum possible score), half of which are threatened with extinction. Although ex-situ conservation efforts and applied research work are needed to safeguard and unlock the full potential of the local endemic NUPs evaluated herein, the proposed multifaceted evaluation scheme revealed that some local endemic NUPs of the studied regions can be sustainably exploited in short- or medium-term, following successful examples of Cretan NUPs e.g., Origanum dictramnus. The sustainable exploitation of high scored taxa of the studied regions can be fastened through targeted species-specific research bridging extant research gaps and facilitating conservation and stakeholder attraction.

The neglected and underutilized plants (NUPs) could become alternative food sources in the agro-alimentary sector, enriching human and animal diets, offering the opportunity for sustainable exploitation, resilience to climate change, and production with resistance to pests and diseases. In the Mediterranean countries, these valuable resources are threatened by climate change, overexploitation, and/or monoculture. In this framework, we evaluated 399 local endemic NUPs of Crete (Greece), the Mediterranean coast, Rif of Morocco, and Tunisia, regarding their agro-alimentary potential, and assessed their feasibility and readiness timescale for sustainable exploitation with own previously published methodology. The methodological scheme was developed by experts in co-creative workshops, using point-scoring of seven attributes to evaluate the potential of the targeted NUPs in the agro-alimentary. Our results showed a diversity of promising local endemic NUPs of different families in the studied regions (Lamiaceae members are prominent), and we outlined the cases of 13 taxa with the highest optimum scores of agro-alimentary potential (>70%). Despite the diversity or the promising potential and current ex-situ conservation efforts to bridge gaps, our study indicated that only a few cases of Cretan local endemic NUPs can be sustainably exploited in the short-term. However, it is argued that many more local endemic NUPs can easily follow sustainable exploitation schemes if specific research gaps are bridged. Since NUPs can help to increased diversification of food production systems by adding new nutritional/beneficial species to human and animal diets, basic and applied research, as well as market and stakeholder attraction, is suggested as prerequisite to unlock the full potential of the focal endemic NUPs in the agro-alimentary sector.

Faba beans are considered one of the most important crops for animal feed. The genotype × environment interaction (GEI) has a considerable effect on faba bean seed production. The objectives of this study included assessing multiple locations and genotypes to understand how various ecosystems and faba bean genotypes relate to one another, and suggesting the ideal climatic conditions, crop management system, and genotypes so that they are carefully chosen for their stability. A 2-year experiment was conducted in order to define the stability across four environments based on stability indices for certain characteristics: moisture (%), ash content (%), crude protein content (%), crude fat (%), total starch (%), and crude fiber content (%). Statistically significant differences indicated that GEIs were present. The heritability was generally high for qualitative traits in comparison with quantitative traits. The crude protein content, plant height, and thousand-seed weight were all positively correlated with the seed yield; however, the other qualitative variables were adversely correlated. The crude protein content of the cultivar Tanagra displayed a high stability index, followed by Ste1. Under conventional management, Tanagra demonstrated high values for the seed yield in Giannitsa and Florina. Ste1 and Ste2 are particularly promising genetic materials that showed high values under low-input conditions. The best genotypes to use and the most favorable environments/types of cultivation were the Tanagra cultivar, followed by the Ste2 genotype, according to the additive main effects and multiplicative interaction (AMMI) and genotype plus genotype-by-environment (GGE) biplot models. Earliness showed significant heritability values and very high stability indices, again indicating qualitative behavior according to genetic parameters. With the exception of the number of pods per plant, which demonstrated low heritability while having excellent index values, traits like seed yield showed relatively low-stability-based heritability values. Global efforts aimed at improving the genetics of faba beans might benefit from genotypes that exhibit consistent yields in various conditions.

The objective of this study was to evaluate the effect of population density and row spacing on field yield and other morphological characteristics of two commercial F1 maize hybrids of different biological cycle (Costanza and LG3535) in a four-year period. Field experiments were conducted in a split-split plot design, in two population densities and three types of row spacing, involving single or twin rows. Ten plants from each plot were selected randomly and plant height, ear emergence height, ear length, ear diameter, number of grain rows per ear, grains number per ear, grain weight per ear, spindle weight per ear, and spindle diameter were measured. Grain yield of each plot was measured and field yield, thousand kernel weight and bulk density were calculated. Four years of experimentation clarified that environmental conditions may distort all other effects of the factors studied. This study points out the best combination of plant density and row configuration. High populations and twin or narrow rows (50 cm) were found to be important for maximizing yield of modern maize hybrids. Differences between hybrids were not significant, although Costanza exhibited greater mean field yield performance (14,364 kg/ha). Plant density was a significant factor and yield was increasing from low to high plant population (from 13,900 to 14,527 kg/ha). Plant density and genetic materials affected thousand kernel weights that showed the highest value at low plant density (364 g). Row spacing showed a significant interaction with year. Generally, twin or narrow rows favored many characteristics, especially height characteristics. Plant height reached 320 cm and ear height reached 149 cm. Ear diameter was favored by low plant density. Some ear characteristics were found to be depended on the genotype behavior in the certain environmental conditions. For spindle weight per ear, a total interaction between years, row spacing, hybrids, and plant density was found. For spindle diameter, various significant interactions were found, including years (maximum also in 2009), row spacing, and plant density and also row spacing, hybrids, and plant density. Many correlations were found significant especially between yield and thousand kernel weight, spindle weight per ear, and ear diameter that may prove to be useful for plant breeders.

In the frame of the sustainable use of neglected and underutilized phytogenetic resources, and along with numerous studies in Abies spp. due to the innate conservation value of fir forests, this research focused on the Moroccan endemic fir, Abies marocana. The aim was triple-fold: to assess its potential and dynamics in economic sectors for sustainable exploitation; to determine the ecological conditions in which the species naturally thrives; and to find the appropriate requirements for its successful seed germination. We sourced multifaceted evaluations for three economic sectors performed in three levels, using 48 attributes and eight criteria from previous studies of our own, and the relevant species-specific assessments are overviewed herein in detail. The species’ ecological profile was constructed using Geographical Information Systems (GIS) and open access data (Worldclim). Seed germination trials were performed to examine the effect of cold stratification (non-stratified, one- and two-months stratified seeds), the influence of four temperatures (10 °C, 15 °C, 20 °C, and 25 °C), and interactions thereof in relation to germination percentage (GP) and mean germination time (MGT). The experiments showed that the interaction of cold stratification and germination temperature has a strong effect on the GP and MGT of A. marocana seeds. A detailed GIS-derived ecological profile of the focal species was created in terms of precipitation and temperature natural regimes, enabling the interpretation of the seed germination results. The multifaceted evaluations reveal an interesting potential of the Moroccan fir in different economic sectors, which is mainly compromised due to extant research gaps, unfavorable conditions, and low stakeholder attraction. The findings of this study fill in extant research gaps, contribute to in situ and ex situ conservation strategies, and can facilitate the sustainable exploitation of this emblematic local endemic plant of northern Morocco.

The primary purpose of this study was to explore yield stability of pea (Pisum sativum L.) cultivars based on stability index, with specific aim at studying cultivar behavior regarding yield of peas under both conventional and low-input cultivation systems. Five cultivars of peas were used in a strip-plot design. Correlations showed a significant positive relation between seed yield and some other traits. Indirect seed yield improvement may be implemented by improving pod length, which generally showed high stability indices in Greek mega-environment. Comparisons between conventional and low-input farming systems generally did not affect stability estimations, but revealed cultivars that exhibited stable performance, even in low-input farming systems. The additive main effects and multiplicative interaction (AMMI) biplot analysis, genotype by environment interaction (GGE) biplot analysis and analysis of variance (ANOVA) showed statistically significant differences between genotypes and environments, and also the farming system. This way, we have certain cultivars of peas to recommend for specific areas and farming system, in order to achieve the most stable performance. Vermio proved to be a stable cultivar for seed yield performance, in Giannitsa, Trikala and Kalambaka area, in low-inputs farming systems, while Olympos was the best in Florina area and low-input farming.