This study endeavored to sustainably control aphids and anthracnose after spraying endophytic Isaria javanica pf185 under field conditions. Under two different tents; one batch of seedlings was sprayed with a 107 conidia/mL I. javanica pf185 suspension; while another was sprayed with 0.05% Tween 80® in distilled water. Six leaf discs from the top; middle; and bottom part of the plant canopy were weekly collected and placed on moistened filter paper in a Petri dish for insecticidal and antifungal bioassays against Myzus persicae and Colletotrichum acutatum. Differences were noticed from the 18th day after spraying with mortality (86.67 ± 0.57% versus 36.67 ± 0.64%) and leaf damage (13.45 ± 0.03% versus 41.18 ± 0.06%) on fungus-treated and controlled, respectively. The corrected insecticidal efficacy was 20.43, 39.82, 72.32, 66.43 and 70.04%, while the corrected fungicidal efficacy was 26.07, 38.01, 53.35, 29.08 and 41.81% during five successive weeks. A positive correlation was evident between insecticidal efficacy and relative humidity (r2 = 0.620) and temperature (r2 = 0.424), respectively. No correlation was found between antifungal activity and relative humidity (r2 = 0.061) and temperature (r2 = 0), respectively. The entomopathogenic fungus survived on leaf surface area and in tissues after spraying.
20 May 2023 in International Journal of Tropical Insect Science
Anti-oviposition induced by Cordyceps fumosorosea FG340 to Spodoptera exigua and its persistence had been assessed on the Chinese cabbage seedlings sprayed with 0, 106, 107 and 108 conidia per mL in greenhouse and laboratory. In a randomized complete block, four couples (males and females) of moths were released in each cage. In the laboratory, the percentage of egg laid varied significantly between treatments with 59.8, 14.8, 11.9, and 13.5% on 0, 106, 107 and 108 conidia per mL; respectively. Similarly, in the greenhouse, the percentage of eggs laid differed significantly between suspensions with 88.18, 8.7, 0, and 3.13% on 0, 106, 107, and 108 conidia per mL; respectively. Repellence study showed that there is a major difference between suspensions with average 34.55, 35.21 and 40.02% for 106, 107 and 108 conidia per mL; respectively. Endophytic C. fumosorosea and its culture fluid affect the behavior of armyworm larvae. The concentration of 108 conidia per mL can affect the oviposition of the armyworm.
31 December 2013 in The Korean Journal of Pesticide Science