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The year 2020 was profoundly marked by the emergence and spread of SARS-CoV-2, causing COVID-19, which represents the greatest pandemic of the 21st century until now, and a major challenge for virologists in the scientific and medical communities. Increased numbers of SARS-CoV-2 infection all over the world imposed social and travel restrictions, including avoidance of face-to-face scientific meetings. Therefore, for the first time in history, the 2020 edition of the Brazilian Society of Virology (SBV) congress was totally online. Despite the challenge of the new format, the Brazilian society board and collaborators were successful in virtually congregating more than 921 attendees, which was the greatest SBV participant number ever reached. Seminal talks from prominent national and international researchers were presented every night, during a week, and included discussions about environmental, basic, animal, human, plant and invertebrate virology. A special roundtable debated exclusively new data and perspectives regarding COVID-19 by some of the greatest Brazilian virologists. Women scientists were very well represented in another special roundtable called “Young Women Inspiring Research”, which was one of the most viewed and commented section during the meeting, given the extraordinary quality of the presented work. Finally, SBV offered the Helio Gelli Pereira award for one graduate and one undergraduate student, which has also been a fruitful collaboration between the society and Viruses journal. The annual SBV meeting has, therefore, reached its goals to inspire young scientists, stimulate high-quality scientific discussion and to encourage global collaboration between virologists.
Luciana Arruda; Fabrício Campos; Jônatas Abrahão; Flávio da Fonseca; João Araújo Junior; Fernando Rosado Spilki. 31st Brazilian Online Society for Virology (SBV) 2020 Annual Meeting. Viruses 2021, 13, 414 .
AMA StyleLuciana Arruda, Fabrício Campos, Jônatas Abrahão, Flávio da Fonseca, João Araújo Junior, Fernando Rosado Spilki. 31st Brazilian Online Society for Virology (SBV) 2020 Annual Meeting. Viruses. 2021; 13 (3):414.
Chicago/Turabian StyleLuciana Arruda; Fabrício Campos; Jônatas Abrahão; Flávio da Fonseca; João Araújo Junior; Fernando Rosado Spilki. 2021. "31st Brazilian Online Society for Virology (SBV) 2020 Annual Meeting." Viruses 13, no. 3: 414.
This Special Issue of Viruses is a collection of the current knowledge on a broad range of emerging human, animal, and plant viral diseases
Fabrício Campos; Luciana de Arruda; Flávio da Fonseca. Special Issue “Emerging Viruses 2020: Surveillance, Prevention, Evolution and Control”. Viruses 2021, 13, 251 .
AMA StyleFabrício Campos, Luciana de Arruda, Flávio da Fonseca. Special Issue “Emerging Viruses 2020: Surveillance, Prevention, Evolution and Control”. Viruses. 2021; 13 (2):251.
Chicago/Turabian StyleFabrício Campos; Luciana de Arruda; Flávio da Fonseca. 2021. "Special Issue “Emerging Viruses 2020: Surveillance, Prevention, Evolution and Control”." Viruses 13, no. 2: 251.
Since exacerbated inflammation and microvascular leakage are hallmarks of dengue virus (DENV) infection, here we interrogated whether systemic activation of the contact/kallikrein-kinin system (KKS) might hamper endothelial function. In vitro assays showed that dextran sulfate, a potent contact activator, failed to generate appreciable levels of activated plasma kallikrein (PKa) in the large majority of samples from a dengue cohort (n = 70), irrespective of severity of clinical symptoms. Impaired formation of PKa in dengue-plasmas correlated with the presence of cleaved Factor XII and high molecular weight kininogen (HK), suggesting that the prothrombogenic contact system is frequently triggered during the course of infection. Using two pathogenic arboviruses, DENV or Zika virus (ZIKV), we then asked whether exogenous BK could influence the outcome of infection of human brain microvascular endothelial cells (HBMECs). Unlike the unresponsive phenotype of Zika-infected HBMECs, we found that BK, acting via B2R, vigorously stimulated DENV-2 replication by reverting nitric oxide-driven apoptosis of endothelial cells. Using the mouse model of cerebral dengue infection, we next demonstrated that B2R targeting by icatibant decreased viral load in brain tissues. In summary, our study suggests that contact/KKS activation followed by BK-induced enhancement of DENV replication in the endothelium may underlie microvascular pathology in dengue.
Sharton V. A. Coelho; Naiara M. Rust; Lucas Vellasco; Michelle P. Papa; Aline S. G. Pereira; Matheus Ferreira Da Silva Palazzo; Maria Aparecida Juliano; Simone M. Costa; Ada M. B. Alves; Marli T. Cordeiro; Ernesto T. A. Marques; Júlio Scharfstein; Luciana B. De Arruda. Contact System Activation in Plasma from Dengue Patients Might Harness Endothelial Virus Replication through the Signaling of Bradykinin Receptors. Pharmaceuticals 2021, 14, 56 .
AMA StyleSharton V. A. Coelho, Naiara M. Rust, Lucas Vellasco, Michelle P. Papa, Aline S. G. Pereira, Matheus Ferreira Da Silva Palazzo, Maria Aparecida Juliano, Simone M. Costa, Ada M. B. Alves, Marli T. Cordeiro, Ernesto T. A. Marques, Júlio Scharfstein, Luciana B. De Arruda. Contact System Activation in Plasma from Dengue Patients Might Harness Endothelial Virus Replication through the Signaling of Bradykinin Receptors. Pharmaceuticals. 2021; 14 (1):56.
Chicago/Turabian StyleSharton V. A. Coelho; Naiara M. Rust; Lucas Vellasco; Michelle P. Papa; Aline S. G. Pereira; Matheus Ferreira Da Silva Palazzo; Maria Aparecida Juliano; Simone M. Costa; Ada M. B. Alves; Marli T. Cordeiro; Ernesto T. A. Marques; Júlio Scharfstein; Luciana B. De Arruda. 2021. "Contact System Activation in Plasma from Dengue Patients Might Harness Endothelial Virus Replication through the Signaling of Bradykinin Receptors." Pharmaceuticals 14, no. 1: 56.
Congenital Zika virus (ZIKV) infection can induce fetal brain abnormalities. Here, we investigated whether maternal ZIKV infection affects placental physiology and metabolic transport potential and impacts the fetal outcome, regardless of viral presence in the fetus at term. Low (103 PFU-ZIKVPE243; low ZIKV) and high (5×107 PFU-ZIKVPE243; high ZIKV) virus titers were injected into immunocompetent (ICompetent C57BL/6) and immunocompromised (ICompromised A129) mice at gestational day (GD) 12.5 for tissue collection at GD18.5 (term). High ZIKV elicited fetal death rates of 66% and 100%, whereas low ZIKV induced fetal death rates of 0% and 60% in C57BL/6 and A129 dams, respectively. All surviving fetuses exhibited intrauterine growth restriction (IUGR) and decreased placental efficiency. High-ZIKV infection in C57BL/6 and A129 mice resulted in virus detection in maternal spleens and placenta, but only A129 fetuses presented virus RNA in the brain. Nevertheless, pregnancies in both strains produced fetuses with decreased head sizes (p<0.05). Low-ZIKV-A129 dams had higher IL-6 and CXCL1 levels (p<0.05), and their placentas showed increased CCL-2 and CXCL-1 contents (p<0.05). In contrast, low-ZIKV-C57BL/6 dams had an elevated CCL2 serum level and increased type I and II IFN expression in the placenta. Notably, less abundant microvilli and mitochondrial degeneration were evidenced in the placental labyrinth zone (Lz) of ICompromised and high-ZIKV-ICompetent mice but not in low-ZIKV-C57BL/6 mice. In addition, decreased placental expression of the drug transporters P-glycoprotein (P-gp) and breast cancer resistance protein (Bcrp) and the lipid transporter Abca1 was detected in all ZIKV-infected groups, but Bcrp and Abca1 were only reduced in ICompromised and high-ZIKV ICompetent mice. Our data indicate that gestational ZIKV infection triggers specific proinflammatory responses and affects placental turnover and transporter expression in a manner dependent on virus concentration and maternal immune status. Placental damage may impair proper fetal-maternal exchange function and fetal growth/survival, likely contributing to congenital Zika syndrome.
Cherley Borba Vieira de Andrade; Victoria Regina De Siqueira Monteiro; Sharton Vinicius Antunes Coelho; Hanailly Ribeiro Gomes; Ronny Paiva Campos de Sousa; Veronica Muller de Oliveira Nascimento; Flavia Fonseca Bloise; Stephen Matthews; Enrrico Bloise; Luciana Barros de Arruda; Tania Maria Ortiga-Carvalho. ZIKV disrupts placental ultrastructure and drug transporter expression in mice. 2020, 1 .
AMA StyleCherley Borba Vieira de Andrade, Victoria Regina De Siqueira Monteiro, Sharton Vinicius Antunes Coelho, Hanailly Ribeiro Gomes, Ronny Paiva Campos de Sousa, Veronica Muller de Oliveira Nascimento, Flavia Fonseca Bloise, Stephen Matthews, Enrrico Bloise, Luciana Barros de Arruda, Tania Maria Ortiga-Carvalho. ZIKV disrupts placental ultrastructure and drug transporter expression in mice. . 2020; ():1.
Chicago/Turabian StyleCherley Borba Vieira de Andrade; Victoria Regina De Siqueira Monteiro; Sharton Vinicius Antunes Coelho; Hanailly Ribeiro Gomes; Ronny Paiva Campos de Sousa; Veronica Muller de Oliveira Nascimento; Flavia Fonseca Bloise; Stephen Matthews; Enrrico Bloise; Luciana Barros de Arruda; Tania Maria Ortiga-Carvalho. 2020. "ZIKV disrupts placental ultrastructure and drug transporter expression in mice." , no. : 1.
The 30th meeting of the Brazilian Society for Virology (SBV) was held, for the first time in its 30 years of existence, in Cuiabá, the capital of Mato Grosso State, Central Western Brazil, a tropical region between the three richest biomes in the world: Amazon Florest, Cerrado and Pantanal. In recent years, the field of virology has been built in the State. The aim of this report is to support participants and virologists to receive the most up-to-date information about the meeting, which occurred from 16 to 19 October 2019. National and international speakers gave SBV the opportunity to learn about their experience on their virology fields, sharing recent scientific findings, compiling conferences, round table presentations and work presentations in oral and poster sessions. The meeting held over 300 attendants, who were also involved on oral and poster presentations, showing a great variety of recent unpublished studies on environmental, basic, animal, human, plant and invertebrate virology. In addition, SBV offered the Helio Gelli Pereira award for the best research studies in each field presented during the meeting. The 30th meeting of SBV was very productive and has also encouraged scientific partnership and collaboration among virologists worldwide.
Renata Dezengrini Slhessarenko; Marcelo Adriano Mendes Dos Santos; Michele Lunardi; Bruno Moreira Carneiro; Juliana Helena Chavez-Pavoni; Daniel Moura De Aguiar; Ana Claudia Pereira Terças Trettel; Carla Regina Andrighetti; Flávio Guimarães Da Fonseca; João Pessoa Araújo Junior; Fabrício Souza Campos; Luciana Barros De Arruda; Jônatas Santos Abrahão; Fernando Rosado Spilki. 30th Brazilian Society for Virology 2019 Annual Meeting—Cuiabá, Mato Grosso, Brazil. Viruses 2020, 12, 494 .
AMA StyleRenata Dezengrini Slhessarenko, Marcelo Adriano Mendes Dos Santos, Michele Lunardi, Bruno Moreira Carneiro, Juliana Helena Chavez-Pavoni, Daniel Moura De Aguiar, Ana Claudia Pereira Terças Trettel, Carla Regina Andrighetti, Flávio Guimarães Da Fonseca, João Pessoa Araújo Junior, Fabrício Souza Campos, Luciana Barros De Arruda, Jônatas Santos Abrahão, Fernando Rosado Spilki. 30th Brazilian Society for Virology 2019 Annual Meeting—Cuiabá, Mato Grosso, Brazil. Viruses. 2020; 12 (5):494.
Chicago/Turabian StyleRenata Dezengrini Slhessarenko; Marcelo Adriano Mendes Dos Santos; Michele Lunardi; Bruno Moreira Carneiro; Juliana Helena Chavez-Pavoni; Daniel Moura De Aguiar; Ana Claudia Pereira Terças Trettel; Carla Regina Andrighetti; Flávio Guimarães Da Fonseca; João Pessoa Araújo Junior; Fabrício Souza Campos; Luciana Barros De Arruda; Jônatas Santos Abrahão; Fernando Rosado Spilki. 2020. "30th Brazilian Society for Virology 2019 Annual Meeting—Cuiabá, Mato Grosso, Brazil." Viruses 12, no. 5: 494.
Emerging viruses represent a major concern for public health offices
Jônatas Santos Abrahão; Luciana Barros De Arruda. Special Issue “Emerging Viruses: Surveillance, Prevention, Evolution, and Control”. Viruses 2020, 12, 306 .
AMA StyleJônatas Santos Abrahão, Luciana Barros De Arruda. Special Issue “Emerging Viruses: Surveillance, Prevention, Evolution, and Control”. Viruses. 2020; 12 (3):306.
Chicago/Turabian StyleJônatas Santos Abrahão; Luciana Barros De Arruda. 2020. "Special Issue “Emerging Viruses: Surveillance, Prevention, Evolution, and Control”." Viruses 12, no. 3: 306.
Human infection by different flaviviruses may cause severe neurologic syndromes, through pathogenic mechanisms that are still largely unknown. Japanese encephalitis virus (JEV), West Nile virus (WNV), Zika virus (ZIKV), yellow fever virus (YFV), dengue virus (DENV), and tick-borne encephalitis virus (TBEV) are believed to reach the central nervous system by a hematogenous route, upon crossing the blood-brain barrier. Although the disruption of BBB during flavivirus infection has been largely evidenced in experimental models, the relevance of BBB breakdown for virus entering the brain was not completely elucidated. In vitro models of BBB had demonstrated that these viruses replicated in brain microvascular endothelial cells (BMECs), which induced downregulation of tight junction proteins and increased the permeability of the barrier. Other reports demonstrated that infection of BMECs allowed the basolateral release of infectious particles, without a remarkable cytopathic effect, what might be sufficient for virus invasion. Virus replication and activation of other cells associated to the BBB, mostly astrocytes and microglia, were also reported to affect the endothelial barrier permeability. This event might occur simultaneously or after BMECs infection, being a secondary effect leading to BBB disruption. Importantly, activation of BMECs, astrocytes, and microglia by flaviviruses was associated to the expression and secretion of inflammatory mediators, which are believed to recruit leukocytes to the CNS. The leukocyte infiltrate could further mediate viral invasion through a Trojan horse mechanism and might contribute to BBB breakdown and to neurological alterations. This review discussed the previous studies regarding in vitro and in vivo models of JEV, WNV, ZIKV, YFV, DENV, and TBEV infection and addressed the pathways for BBB overcome and invasion of the CNS described for each virus infection, aiming to increment the knowledge and stimulate further discussion about the role of BBB in the neuropathogenesis of flavivirus infection.
Yasmin Mucunã Mustafá; Lana Monteiro Meuren; Sharton Coelho; Luciana Barros De Arruda. Pathways Exploited by Flaviviruses to Counteract the Blood-Brain Barrier and Invade the Central Nervous System. Frontiers in Microbiology 2019, 10, 525 .
AMA StyleYasmin Mucunã Mustafá, Lana Monteiro Meuren, Sharton Coelho, Luciana Barros De Arruda. Pathways Exploited by Flaviviruses to Counteract the Blood-Brain Barrier and Invade the Central Nervous System. Frontiers in Microbiology. 2019; 10 ():525.
Chicago/Turabian StyleYasmin Mucunã Mustafá; Lana Monteiro Meuren; Sharton Coelho; Luciana Barros De Arruda. 2019. "Pathways Exploited by Flaviviruses to Counteract the Blood-Brain Barrier and Invade the Central Nervous System." Frontiers in Microbiology 10, no. : 525.
Chronic immune activation is a hallmark of HIV infection and is often not controlled even in patients under antiretroviral therapy. Indeed, chronic diseases with inflammatory pathogenesis are being reported as major causes of death for HIV-infected persons. Hydroxypropyl-beta cyclodextrin (HP-BCD) is a cholesterol-sequestering drug that inhibits HIV replication and infectivity in vitro and in vivo . Recent studies have demonstrated the importance of cholesterol metabolism and content in different inflammatory conditions; therefore, we investigated the potential of HP-BCD as an immunomodulatory drug, regulating the activation of cells from HIV-infected patients. Treatment of monocytes with HP-BCD inhibited the expression and secretion of receptors and mediators that are usually enhanced in HIV patients. Furthermore, we investigated the molecular mechanisms associated with the immunomodulatory effect of HP-BCD. Our results indicate that, besides reducing viral replication, HP-BCD treatment may contribute to modulation of chronic immune activation associated with AIDS.
Flávio Lemos Matassoli; Ihid Carneiro Leão; Bruno Braz Bezerra; Richard B. Pollard; Dieter Lütjohann; James E. K. Hildreth; Luciana Barros de Arruda. Hydroxypropyl-Beta-Cyclodextrin Reduces Inflammatory Signaling from Monocytes: Possible Implications for Suppression of HIV Chronic Immune Activation. mSphere 2018, 3, e00497-18 .
AMA StyleFlávio Lemos Matassoli, Ihid Carneiro Leão, Bruno Braz Bezerra, Richard B. Pollard, Dieter Lütjohann, James E. K. Hildreth, Luciana Barros de Arruda. Hydroxypropyl-Beta-Cyclodextrin Reduces Inflammatory Signaling from Monocytes: Possible Implications for Suppression of HIV Chronic Immune Activation. mSphere. 2018; 3 (6):e00497-18.
Chicago/Turabian StyleFlávio Lemos Matassoli; Ihid Carneiro Leão; Bruno Braz Bezerra; Richard B. Pollard; Dieter Lütjohann; James E. K. Hildreth; Luciana Barros de Arruda. 2018. "Hydroxypropyl-Beta-Cyclodextrin Reduces Inflammatory Signaling from Monocytes: Possible Implications for Suppression of HIV Chronic Immune Activation." mSphere 3, no. 6: e00497-18.
The dynamics of dengue virus (DENV) circulation depends on serotype, genotype and lineage replacement and turnover. In São José do Rio Preto, Brazil, we observed that the L6 lineage of DENV-1 (genotype V) remained the dominant circulating lineage even after the introduction of the L1 lineage. We investigated viral fitness and immunogenicity of the L1 and L6 lineages and which factors interfered with the dynamics of DENV epidemics. The results showed a more efficient replicative fitness of L1 over L6 in mosquitoes and in human and non-human primate cell lines. Infections by the L6 lineage were associated with reduced antigenicity, weak B and T cell stimulation and weak host immune system interactions, which were associated with higher viremia. Our data, therefore, demonstrate that reduced viral immunogenicity and consequent greater viremia determined the increased epidemiological fitness of DENV-1 L6 lineage in São José do Rio Preto.
Tauyne Menegaldo Pinheiro; Mânlio Tasso De Oliveira Mota; Aripuanã Sakurada Aranha Watanabe; Joice Matos Biselli; Betânia Paiva Drumond; Milene Rocha Ribeiro; Danila Vedovello; João Pessoa Araújo; Paulo Filemon Paolucci Pimenta; Bárbara Aparecida Chaves; Mayara Marques Carneiro Da Silva; Izabella Cristina Andrade Batista; Michelle Premazzi Papa; Lana Monteiro Meuren; Carolina Gonçalves De Oliveira Lucas; Flavio Lemos Matassoli; Laura Helena Vega Gonzales Gil; Adriana Bozzi; Carlos Eduardo Calzavara-Silva; Luciana Barros De Arruda; Danielle Da Glória De Souza; Mauro Martins Teixeira; Nikos Vasilakis; Maurício Lacerda Nogueira. Viral immunogenicity determines epidemiological fitness in a cohort of DENV-1 infection in Brazil. PLOS Neglected Tropical Diseases 2018, 12, e0006525 .
AMA StyleTauyne Menegaldo Pinheiro, Mânlio Tasso De Oliveira Mota, Aripuanã Sakurada Aranha Watanabe, Joice Matos Biselli, Betânia Paiva Drumond, Milene Rocha Ribeiro, Danila Vedovello, João Pessoa Araújo, Paulo Filemon Paolucci Pimenta, Bárbara Aparecida Chaves, Mayara Marques Carneiro Da Silva, Izabella Cristina Andrade Batista, Michelle Premazzi Papa, Lana Monteiro Meuren, Carolina Gonçalves De Oliveira Lucas, Flavio Lemos Matassoli, Laura Helena Vega Gonzales Gil, Adriana Bozzi, Carlos Eduardo Calzavara-Silva, Luciana Barros De Arruda, Danielle Da Glória De Souza, Mauro Martins Teixeira, Nikos Vasilakis, Maurício Lacerda Nogueira. Viral immunogenicity determines epidemiological fitness in a cohort of DENV-1 infection in Brazil. PLOS Neglected Tropical Diseases. 2018; 12 (5):e0006525.
Chicago/Turabian StyleTauyne Menegaldo Pinheiro; Mânlio Tasso De Oliveira Mota; Aripuanã Sakurada Aranha Watanabe; Joice Matos Biselli; Betânia Paiva Drumond; Milene Rocha Ribeiro; Danila Vedovello; João Pessoa Araújo; Paulo Filemon Paolucci Pimenta; Bárbara Aparecida Chaves; Mayara Marques Carneiro Da Silva; Izabella Cristina Andrade Batista; Michelle Premazzi Papa; Lana Monteiro Meuren; Carolina Gonçalves De Oliveira Lucas; Flavio Lemos Matassoli; Laura Helena Vega Gonzales Gil; Adriana Bozzi; Carlos Eduardo Calzavara-Silva; Luciana Barros De Arruda; Danielle Da Glória De Souza; Mauro Martins Teixeira; Nikos Vasilakis; Maurício Lacerda Nogueira. 2018. "Viral immunogenicity determines epidemiological fitness in a cohort of DENV-1 infection in Brazil." PLOS Neglected Tropical Diseases 12, no. 5: e0006525.
Zika virus (ZIKV) has been associated to central nervous system (CNS) harm, and virus was detected in the brain and cerebrospinal fluids of microcephaly and meningoencephalitis cases. However, the mechanism by which the virus reaches the CNS is unclear. Here, we addressed the effects of ZIKV replication in human brain microvascular endothelial cells (HBMECs), as an in vitro model of blood brain barrier (BBB), and evaluated virus extravasation and BBB integrity in an in vivo mouse experimental model. HBMECs were productively infected by African and Brazilian ZIKV strains (ZIKVMR766 and ZIKVPE243), which induce increased production of type I and type III IFN, inflammatory cytokines and chemokines. Infection with ZIKVMR766 promoted earlier cellular death, in comparison to ZIKVPE243, but infection with either strain did not result in enhanced endothelial permeability. Despite the maintenance of endothelial integrity, infectious virus particles crossed the monolayer by endocytosis/exocytosis-dependent replication pathway or by transcytosis. Remarkably, both viruses' strains infected IFNAR deficient mice, with high viral load being detected in the brains, without BBB disruption, which was only detected at later time points after infection. These data suggest that ZIKV infects and activates endothelial cells, and might reach the CNS through basolateral release, transcytosis or transinfection processes. These findings further improve the current knowledge regarding ZIKV dissemination pathways.
Michelle P. Papa; Lana M. Meuren; Sharton V. A. Coelho; Carolina G. De Oliveira Lucas; Yasmin M. Mustafá; Flavio Lemos Matassoli; Paola P. Silveira; Paula S. Frost; Paula Pezzuto; Milene R. Ribeiro; Amilcar Tanuri; Mauricio L. Nogueira; Loraine Campanati; Marcelo T. Bozza; Heitor A. Paula Neto; Pedro M. Pimentel-Coelho; Claudia P. Figueiredo; Renato S. de Aguiar; Luciana B. de Arruda. Zika Virus Infects, Activates, and Crosses Brain Microvascular Endothelial Cells, without Barrier Disruption. Frontiers in Microbiology 2017, 8, 2557 .
AMA StyleMichelle P. Papa, Lana M. Meuren, Sharton V. A. Coelho, Carolina G. De Oliveira Lucas, Yasmin M. Mustafá, Flavio Lemos Matassoli, Paola P. Silveira, Paula S. Frost, Paula Pezzuto, Milene R. Ribeiro, Amilcar Tanuri, Mauricio L. Nogueira, Loraine Campanati, Marcelo T. Bozza, Heitor A. Paula Neto, Pedro M. Pimentel-Coelho, Claudia P. Figueiredo, Renato S. de Aguiar, Luciana B. de Arruda. Zika Virus Infects, Activates, and Crosses Brain Microvascular Endothelial Cells, without Barrier Disruption. Frontiers in Microbiology. 2017; 8 ():2557.
Chicago/Turabian StyleMichelle P. Papa; Lana M. Meuren; Sharton V. A. Coelho; Carolina G. De Oliveira Lucas; Yasmin M. Mustafá; Flavio Lemos Matassoli; Paola P. Silveira; Paula S. Frost; Paula Pezzuto; Milene R. Ribeiro; Amilcar Tanuri; Mauricio L. Nogueira; Loraine Campanati; Marcelo T. Bozza; Heitor A. Paula Neto; Pedro M. Pimentel-Coelho; Claudia P. Figueiredo; Renato S. de Aguiar; Luciana B. de Arruda. 2017. "Zika Virus Infects, Activates, and Crosses Brain Microvascular Endothelial Cells, without Barrier Disruption." Frontiers in Microbiology 8, no. : 2557.
The emergence of Zika virus (ZIKV) infection has stimulated several research groups to study and collaborate to understand virus biology and pathogenesis. These efforts may assist with the development of antiviral drugs, vaccines and diagnostic tests, as well as to promote advancements in public health policies. Here, we aim to develop standard protocols for propagation, titration, and purification of ZIKV strains, by systematically testing different cell types, kinetics, multiplicity of infection and centrifugation protocols. ZIKV produces a productive infection in human, non-human primate, and rodents-derived cell lines, with different efficacies. The highest yield of ZIKV-AFR and ZIKV-BR infectious progeny was obtained at 7days post infection in C6/36 cells (7×10 and 2×10 PFU/ml, respectively). However, high titers of ZIKV-AFR could be obtained at earlier time points in Vero cells (2.5×10PFU/ml at 72hpi), whereas ZIKV-BR titers reached 10 PFU/ml at 4dpi in C6/36 cells. High yield of purified virus was obtained by purification through a discontinuous sucrose gradient. This optimized procedure will certainly contribute to future studies of virus structure and vaccine development. Beyond the achievement of efficient virus propagation, the normalization of these protocols will also allow different laboratories around the world to better compare and discuss data regarding different features of ZIKV biology and disease, contributing to more efficient collaborations and progression in ZIKV research.
Sharton Vinicius Antunes Coelho; Rômulo Leão Silva Neris; Michelle Premazzi Papa; Laila Castro Schnellrath; Lana Monteiro Meuren; Diogo A. Tschoeke; Luciana Leomil; Brunno Verçoza; Milene Miranda; Fabiano L. Thompson; Andrea Thompson Da Poian; Thiago Souza; Fabiana Carneiro; Clarissa Damaso; Iranaia Assunção-Miranda; Luciana Barros de Arruda. Development of standard methods for Zika virus propagation, titration, and purification. Journal of Virological Methods 2017, 246, 65 -74.
AMA StyleSharton Vinicius Antunes Coelho, Rômulo Leão Silva Neris, Michelle Premazzi Papa, Laila Castro Schnellrath, Lana Monteiro Meuren, Diogo A. Tschoeke, Luciana Leomil, Brunno Verçoza, Milene Miranda, Fabiano L. Thompson, Andrea Thompson Da Poian, Thiago Souza, Fabiana Carneiro, Clarissa Damaso, Iranaia Assunção-Miranda, Luciana Barros de Arruda. Development of standard methods for Zika virus propagation, titration, and purification. Journal of Virological Methods. 2017; 246 ():65-74.
Chicago/Turabian StyleSharton Vinicius Antunes Coelho; Rômulo Leão Silva Neris; Michelle Premazzi Papa; Laila Castro Schnellrath; Lana Monteiro Meuren; Diogo A. Tschoeke; Luciana Leomil; Brunno Verçoza; Milene Miranda; Fabiano L. Thompson; Andrea Thompson Da Poian; Thiago Souza; Fabiana Carneiro; Clarissa Damaso; Iranaia Assunção-Miranda; Luciana Barros de Arruda. 2017. "Development of standard methods for Zika virus propagation, titration, and purification." Journal of Virological Methods 246, no. : 65-74.
Flaviviruses, from Flaviviridae virus family, comprises several human pathogens, including Dengue, Zika, Yellow Fever, West Nile and Japanese Encephalitis viruses. Those are enveloped, single-stranded positive sense RNA viruses, and replicate mostly in intracellular compartments associated to endoplasmic reticulum (ER) and Golgi complex. Virus replication results in abundant viral RNAs and proteins, which are recognized by cellular mechanisms evolved to prevent virus infection, resulting in inflammation and stress responses. Virus RNA molecules are sensed by Toll-like receptors (TLRs), RIG-I-like receptors (RIG-I and MDA5) and RNA-dependent protein kinases (PKR), inducing the production of inflammatory mediators and interferons. Simultaneously, the synthesis of virus RNA and proteins are distinguished in different compartments such as mitochondria, ER and cytoplasmic granules, triggering intracellular stress pathways, including oxidative stress, UPR pathway, and stress granules assembly. Here, we review the new findings that connect the inflammatory pathways to cellular stress sensors and the strategies of Flaviviridae members to counteract these cellular mechanisms and escape immune response.
Ana L. C. Valadão; Renato Aguiar; Luciana B. De Arruda. Interplay between Inflammation and Cellular Stress Triggered by Flaviviridae Viruses. Frontiers in Microbiology 2016, 7, 1233 -1233.
AMA StyleAna L. C. Valadão, Renato Aguiar, Luciana B. De Arruda. Interplay between Inflammation and Cellular Stress Triggered by Flaviviridae Viruses. Frontiers in Microbiology. 2016; 7 ():1233-1233.
Chicago/Turabian StyleAna L. C. Valadão; Renato Aguiar; Luciana B. De Arruda. 2016. "Interplay between Inflammation and Cellular Stress Triggered by Flaviviridae Viruses." Frontiers in Microbiology 7, no. : 1233-1233.
The decline in number and function of T cells is a hallmark of HIV infection, and preservation or restoration of HIV-specific cellular immune response is a major goal of AIDS treatment. Dendritic cells (DCs) play a key role in the initiation and maintenance of the immune response, and their use as a vaccine vehicle is a promising strategy for enhancing vaccine efficacy. We evaluated the potential of DC-mediated immunization with a DNA vaccine consisting of HIV-1-p55gag (gag, group-specific antigen) associated to lysosomal associated protein (LAMP) sequence (LAMP/gag vaccine). Immunization of mice with mouse DCs transfected with LAMP/gag (Lg-mDCs) stimulated more potent B- and T-cell responses than naked DNA or DCs pulsed with inactivated HIV. Anti-Gag antibody levels were sustained for at least 3 mo after immunization, and recall T-cell responses were also strongly detected at this time point. Human DCs transfected with LAMP/gag (Lg-hDCs) were also activated and able to stimulate greater T-cell response than native gag-transfected DCs. Coculture between Lg-hDCs and T lymphocytes obtained from patients with HIV resulted in upregulation of CD38, CD69, HLA-DR, and granzyme B by CD4+ and CD8+ T cells, and increased IFN-γ and TNF-α production. These results indicate that the use of LAMP/gag-DC may be an efficient strategy for enhancing immune function in patients with HIV.—Lucas, C. G. D. O., Matassoli, F. L., Peçanha, L. M. T., Santillo, B. T., Oliveira, L. M. D. S., Oshiro, T. M., Marques, E. T. D. A., Jr., Oxenius, A., de Arruda, L. B. Dendritic cells primed with a chimeric plasmid containing HIV-1-gag associated with lysosomal-associated protein-1 (LAMP/gag) is a potential therapeutic vaccine against HIV. FASEB J. 30, 2970-2984 (2016). www.fasebj.org
Carolina G. D. O. Lucas; Flavio L. Matassoli; Ligia M. T. Peçanha; Bruna Tereso Santillo; Luanda Mara Da Silva Oliveira; Telma Miyuki Oshiro; Ernesto T. D. A. Marques; Annette Oxenius; Luciana B. de Arruda. Dendritic cells primed with a chimeric plasmid containing HIV‐1‐ gag associated with lysosomal‐associated protein‐1 (LAMP/ gag ) is a potential therapeutic vaccine against HIV. The FASEB Journal 2016, 30, 2970 -2984.
AMA StyleCarolina G. D. O. Lucas, Flavio L. Matassoli, Ligia M. T. Peçanha, Bruna Tereso Santillo, Luanda Mara Da Silva Oliveira, Telma Miyuki Oshiro, Ernesto T. D. A. Marques, Annette Oxenius, Luciana B. de Arruda. Dendritic cells primed with a chimeric plasmid containing HIV‐1‐ gag associated with lysosomal‐associated protein‐1 (LAMP/ gag ) is a potential therapeutic vaccine against HIV. The FASEB Journal. 2016; 30 (8):2970-2984.
Chicago/Turabian StyleCarolina G. D. O. Lucas; Flavio L. Matassoli; Ligia M. T. Peçanha; Bruna Tereso Santillo; Luanda Mara Da Silva Oliveira; Telma Miyuki Oshiro; Ernesto T. D. A. Marques; Annette Oxenius; Luciana B. de Arruda. 2016. "Dendritic cells primed with a chimeric plasmid containing HIV‐1‐ gag associated with lysosomal‐associated protein‐1 (LAMP/ gag ) is a potential therapeutic vaccine against HIV." The FASEB Journal 30, no. 8: 2970-2984.
Dengue infection is associated to vigorous inflammatory response, to a high frequency of activated B cells, and to increased levels of circulating cross-reactive antibodies. We investigated whether direct infection of B cells would promote activation by culturing primary human B lymphocytes from healthy donors with DENV in vitro. B cells were susceptible, but poorly permissive to infection. Even though, primary B cells cultured with DENV induced substantial IgM secretion, which is a hallmark of polyclonal B cell activation. Notably, DENV induced the activation of B cells obtained from either DENV immune or DENV naïve donors, suggesting that it was not dependent on DENV-specific secondary/memory response. B cell stimulation was dependent on activation of MAPK and CD81. B cells cultured with DENV also secreted IL-6 and presented increased expression of CD86 and HLA-DR, which might contribute to B lymphocyte co-stimulatory function. Indeed, PBMCs, but not isolated B cells, secreted high amounts of IgG upon DENV culture, suggesting that interaction with other cell types in vivo might promote Ig isotype switching and IgG secretion from different B cell clones. These findings suggest that activation signaling pathways triggered by DENV interaction with non-specific receptors on B cells might contribute to the exacerbated response observed in dengue patients.
Arturo Ramon Vargas Correa; Ana Carolina Egypto Rosa Berbel; Michelle Premazzi Papa; Ana Theresa Silveira De Morais; Ligia Maria Torres Peçanha; Luciana Barros De Arruda. Dengue Virus Directly Stimulates Polyclonal B Cell Activation. PLoS ONE 2015, 10, e0143391 .
AMA StyleArturo Ramon Vargas Correa, Ana Carolina Egypto Rosa Berbel, Michelle Premazzi Papa, Ana Theresa Silveira De Morais, Ligia Maria Torres Peçanha, Luciana Barros De Arruda. Dengue Virus Directly Stimulates Polyclonal B Cell Activation. PLoS ONE. 2015; 10 (12):e0143391.
Chicago/Turabian StyleArturo Ramon Vargas Correa; Ana Carolina Egypto Rosa Berbel; Michelle Premazzi Papa; Ana Theresa Silveira De Morais; Ligia Maria Torres Peçanha; Luciana Barros De Arruda. 2015. "Dengue Virus Directly Stimulates Polyclonal B Cell Activation." PLoS ONE 10, no. 12: e0143391.
We have previously demonstrated that a DNA vaccine encoding HIV-p55gag in association with the lysosomal associated membrane protein-1 (LAMP-1) elicited a greater Gag-specific immune response, in comparison to a DNA encoding the native gag. In vitro studies have also demonstrated that LAMP/Gag was highly expressed and was present in MHCII containing compartments in transfected cells. In this study, the mechanisms involved in these processes and the relative contributions of the increased expression and altered traffic for the enhanced immune response were addressed. Cells transfected with plasmid DNA constructs containing p55gag attached to truncated sequences of LAMP-1 showed that the increased expression of gag mRNA required p55gag in frame with at least 741 bp of the LAMP-1 luminal domain. LAMP luminal domain also showed to be essential for Gag traffic through lysosomes and, in this case, the whole sequence was required. Further analysis of the trafficking pathway of the intact LAMP/Gag chimera demonstrated that it was secreted, at least in part, associated with exosome-like vesicles. Immunization of mice with LAMP/gag chimeric plasmids demonstrated that high expression level alone can induce a substantial transient antibody response, but targeting of the antigen to the endolysosomal/secretory pathways was required for establishment of cellular and memory response. The intact LAMP/gag construct induced polyfunctional CD4+ T cell response, which presence at the time of immunization was required for CD8+ T cell priming. LAMP-mediated targeting to endolysosomal/secretory pathway is an important new mechanistic element in LAMP-mediated enhanced immunity with applications to the development of novel anti-HIV vaccines and to general vaccinology field.
Rodrigo Maciel Da Costa Godinho; Flavio Lemos Matassoli; Carolina Gonçalves De Oliveira Lucas; Paula Ordonhez Rigato; Jorge Luiz Santos Gonçalves; Maria Notomi Sato; Milton Maciel; Ligia Maria Torres Peçanha; J. Thomas August; Ernesto Marques; Luciana Barros De Arruda. Regulation of HIV-Gag Expression and Targeting to the Endolysosomal/Secretory Pathway by the Luminal Domain of Lysosomal-Associated Membrane Protein (LAMP-1) Enhance Gag-Specific Immune Response. PLOS ONE 2014, 9, e99887 .
AMA StyleRodrigo Maciel Da Costa Godinho, Flavio Lemos Matassoli, Carolina Gonçalves De Oliveira Lucas, Paula Ordonhez Rigato, Jorge Luiz Santos Gonçalves, Maria Notomi Sato, Milton Maciel, Ligia Maria Torres Peçanha, J. Thomas August, Ernesto Marques, Luciana Barros De Arruda. Regulation of HIV-Gag Expression and Targeting to the Endolysosomal/Secretory Pathway by the Luminal Domain of Lysosomal-Associated Membrane Protein (LAMP-1) Enhance Gag-Specific Immune Response. PLOS ONE. 2014; 9 (6):e99887.
Chicago/Turabian StyleRodrigo Maciel Da Costa Godinho; Flavio Lemos Matassoli; Carolina Gonçalves De Oliveira Lucas; Paula Ordonhez Rigato; Jorge Luiz Santos Gonçalves; Maria Notomi Sato; Milton Maciel; Ligia Maria Torres Peçanha; J. Thomas August; Ernesto Marques; Luciana Barros De Arruda. 2014. "Regulation of HIV-Gag Expression and Targeting to the Endolysosomal/Secretory Pathway by the Luminal Domain of Lysosomal-Associated Membrane Protein (LAMP-1) Enhance Gag-Specific Immune Response." PLOS ONE 9, no. 6: e99887.