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Pseudomonas citronellolis SJTE-3 was isolated as a highly efficient microorganism for biodegradation and valorization of drilling fluids (DF) wastewater. The strain metabolised DF and oily mud exhibiting up to 93%, 86%, 85% and 88% of chemical oxygen demand (COD), n-dodecane, n-tetradecane and naphthalene removal efficiency respectively. Enhanced bioconversion was enabled through production of biosurfactants that reduced the surface tension of water by 53% and resulted in 43.3% emulsification index (E24), while synthesizing 24% of dry cell weight (DCW) as medium-chain-length polyhydroxyalkanoates (PHA). Expression from the main pathways for alkanes and naphthalene biodegradation as well as biosurfactants and PHA biosynthesis revealed that although the alkanes and naphthalene biodegradation routes were actively expressed even at stationary phase, PHA production was stimulated at late stationary phase and putisolvin could comprise the biosurfactant synthesized. The bioconversion of toxic petrochemical residues to added-value thermoelastomers and biosurfactants indicate the high industrial significance of P. citronellolis SJTE-3.
Michalis Koutinas; Maria Kyriakou; Kostas Andreou; Michalis Hadjicharalambous; Efstathios Kaliviotis; Dimitris Pasias; George Kazamias; Costas Varavvas; Ioannis Vyrides. Enhanced biodegradation and valorization of drilling wastewater via simultaneous production of biosurfactants and polyhydroxyalkanoates by Pseudomonas citronellolis SJTE-3. Bioresource Technology 2021, 340, 125679 .
AMA StyleMichalis Koutinas, Maria Kyriakou, Kostas Andreou, Michalis Hadjicharalambous, Efstathios Kaliviotis, Dimitris Pasias, George Kazamias, Costas Varavvas, Ioannis Vyrides. Enhanced biodegradation and valorization of drilling wastewater via simultaneous production of biosurfactants and polyhydroxyalkanoates by Pseudomonas citronellolis SJTE-3. Bioresource Technology. 2021; 340 ():125679.
Chicago/Turabian StyleMichalis Koutinas; Maria Kyriakou; Kostas Andreou; Michalis Hadjicharalambous; Efstathios Kaliviotis; Dimitris Pasias; George Kazamias; Costas Varavvas; Ioannis Vyrides. 2021. "Enhanced biodegradation and valorization of drilling wastewater via simultaneous production of biosurfactants and polyhydroxyalkanoates by Pseudomonas citronellolis SJTE-3." Bioresource Technology 340, no. : 125679.
A process for the valorization of citrus peel waste (CPW) has been developed aiming to produce succinic acid and a series of added-value products through the biorefinery platform. CPW was subject to physicochemical and biological treatment to isolate essential oils (0.43%) and pectin (30.53%) as extractable products, pretreating the material for subsequent production of succinic acid that enabled application of remaining biorefinery residues (BR) as fertilizer substitute. Cellulose, hemicellulose and lignin contents of CPW accounted for 22.45%, 8.05% and 0.66% respectively, while acid hydrolysis reduced hemicellulose by 3.42% in BR. Moreover, essential oils extracted from CPW included 17 compounds, among which D-limonene reached 96.7%. The hydrolyzate generated was fermented for succinic acid production using Actinobacillus succinogenes. Different batch experiments demonstrated that the combined use of corn steep liquor (CSL) and vitamins in a lab-scale bioreactor resulted in product concentration and yield that reached 18.5 g L−1 and 0.62 g g−1 respectively. Although simultaneous saccharification and fermentation (SSF) could not enhance succinic acid production, a fed-batch fermentation strategy increased succinic acid concentration and yield generating 22.4 g L−1 and 0.73 g g−1 respectively, while the mass of the platform chemical formed was enhanced by 27% as compared to the batch process. BR was explored as fertilizer substitute aiming to close the loop in the management of CPW towards development of a zero-waste process demonstrating that although the material imposed stress on plant growth, the content of potassium, phosphorus and nitrogen in the mixture increased.
Maria Patsalou; Antonios Chrysargyris; Nikolaos Tzortzakis; Michalis Koutinas. A biorefinery for conversion of citrus peel waste into essential oils, pectin, fertilizer and succinic acid via different fermentation strategies. Waste Management 2020, 113, 469 -477.
AMA StyleMaria Patsalou, Antonios Chrysargyris, Nikolaos Tzortzakis, Michalis Koutinas. A biorefinery for conversion of citrus peel waste into essential oils, pectin, fertilizer and succinic acid via different fermentation strategies. Waste Management. 2020; 113 ():469-477.
Chicago/Turabian StyleMaria Patsalou; Antonios Chrysargyris; Nikolaos Tzortzakis; Michalis Koutinas. 2020. "A biorefinery for conversion of citrus peel waste into essential oils, pectin, fertilizer and succinic acid via different fermentation strategies." Waste Management 113, no. : 469-477.
Maria Patsalou; Antonios Chrysargyris; Nikolaos Tzortzakis; Michalis Koutinas. A biorefinery for conversion of citrus peel waste into essential oils, pectin, fertilizer and succinic acid via different fermentation strategies. 2020, 113, 469 -477.
AMA StyleMaria Patsalou, Antonios Chrysargyris, Nikolaos Tzortzakis, Michalis Koutinas. A biorefinery for conversion of citrus peel waste into essential oils, pectin, fertilizer and succinic acid via different fermentation strategies. . 2020; 113 ():469-477.
Chicago/Turabian StyleMaria Patsalou; Antonios Chrysargyris; Nikolaos Tzortzakis; Michalis Koutinas. 2020. "A biorefinery for conversion of citrus peel waste into essential oils, pectin, fertilizer and succinic acid via different fermentation strategies." 113, no. : 469-477.
A novel method for enhancement of ethanol production and temperature tolerance of S. cerevisiae through the development of biochar-based biocatalysts (BBBs) is reported. Immobilized BBBs were applied in alcoholic fermentations of hydrolyzates generated via a citrus peel waste (CPW) biorefinery, which allowed extraction of high-purity pectin that reached 30.5% (w/w). Pistachio-nut shells, peanut shells and corks were employed for biochar generation via pyrolysis to produce the cell carriers required. All materials were highly carbonaceous with mesopore size structures (1–50 μm), while peanut shells biochar was crystalline incorporating calcite and sylvite. S. cerevisiae immobilized on pistachio-nuts biochar grown on a synthetic CPW hydrolysate, exhibited 63 g L−1 ethanol concentration and 7.9 g L−1 h−1 productivity improving substantially biosystem performance as compared to unsupported cultures. Alcoholic fermentations conducted at different elevated temperatures (37–41 °C) exhibited stable performance of the immobilized system for six repeated batch experiments. Fermentations of the CPW-hydrolyzate formed through the biorefinery at 41 °C using BBB produced 30.8 g L−1 of ethanol, while free cells achieved significantly lower concentration (13.4 g L−1). The proposed technology confers thermotolerance on S. cerevisiae, which buffers the negative impact of high temperatures on cells leading in increased bioethanol production and lower energy demand.
Maria Kyriakou; Maria Patsalou; Nikolas Xiaris; Athanasios Tsevis; Loukas Koutsokeras; Georgios Constantinides; Michalis Koutinas. Enhancing bioproduction and thermotolerance in Saccharomyces cerevisiae via cell immobilization on biochar: Application in a citrus peel waste biorefinery. Renewable Energy 2020, 155, 53 -64.
AMA StyleMaria Kyriakou, Maria Patsalou, Nikolas Xiaris, Athanasios Tsevis, Loukas Koutsokeras, Georgios Constantinides, Michalis Koutinas. Enhancing bioproduction and thermotolerance in Saccharomyces cerevisiae via cell immobilization on biochar: Application in a citrus peel waste biorefinery. Renewable Energy. 2020; 155 ():53-64.
Chicago/Turabian StyleMaria Kyriakou; Maria Patsalou; Nikolas Xiaris; Athanasios Tsevis; Loukas Koutsokeras; Georgios Constantinides; Michalis Koutinas. 2020. "Enhancing bioproduction and thermotolerance in Saccharomyces cerevisiae via cell immobilization on biochar: Application in a citrus peel waste biorefinery." Renewable Energy 155, no. : 53-64.
Background Lupanine is a plant toxin contained in the wastewater of lupine bean processing industries, which could be used for semi-synthesis of various novel high added-value compounds. This paper introduces an environmental friendly process for microbial production of enantiopure lupanine. Results Previously isolated P. putida LPK411, R. rhodochrous LPK211 and Rhodococcus sp. LPK311, holding the capacity to utilize lupanine as single carbon source, were employed as biocatalysts for resolution of racemic lupanine. All strains achieved high enantiomeric excess (ee) of L-(−)-lupanine (> 95%), while with the use of LPK411 53% of the initial racemate content was not removed. LPK411 fed with lupanine enantiomers as single substrates achieved 92% of D-(+)-lupanine biodegradation, whereas L-(−)-lupanine was not metabolized. Monitoring the transcriptional kinetics of the luh gene in cultures supplemented with the racemate as well as each of the enantiomers supported the enantioselectivity of LPK411 for D-(+)-lupanine biotransformation, while (trans)-6-oxooctahydro-1H-quinolizine-3-carboxylic acid was detected as final biodegradation product from D-(+)-lupanine use. Ecotoxicological assessment demonstrated that lupanine enantiomers were less toxic to A. fischeri compared to the racemate exhibiting synergistic interaction. Conclusions The biological chiral separation process of lupanine presented here constitutes an eco-friendly and low-cost alternative to widely used chemical methods for chiral separation.
Stella Parmaki; Argyro Tsipa; Marlen I. Vasquez; João M. J. Gonçalves; Ioanna Hadjiadamou; Frederico Castelo Ferreira; Carlos A. M. Afonso; Chrysoulla Drouza; Michalis Koutinas. Resolution of alkaloid racemate: a novel microbial approach for the production of enantiopure lupanine via industrial wastewater valorization. Microbial Cell Factories 2020, 19, 1 -10.
AMA StyleStella Parmaki, Argyro Tsipa, Marlen I. Vasquez, João M. J. Gonçalves, Ioanna Hadjiadamou, Frederico Castelo Ferreira, Carlos A. M. Afonso, Chrysoulla Drouza, Michalis Koutinas. Resolution of alkaloid racemate: a novel microbial approach for the production of enantiopure lupanine via industrial wastewater valorization. Microbial Cell Factories. 2020; 19 (1):1-10.
Chicago/Turabian StyleStella Parmaki; Argyro Tsipa; Marlen I. Vasquez; João M. J. Gonçalves; Ioanna Hadjiadamou; Frederico Castelo Ferreira; Carlos A. M. Afonso; Chrysoulla Drouza; Michalis Koutinas. 2020. "Resolution of alkaloid racemate: a novel microbial approach for the production of enantiopure lupanine via industrial wastewater valorization." Microbial Cell Factories 19, no. 1: 1-10.
This paper deals with the development of a citrus peel waste (CPW) biorefinery that employs low environmental impact technologies for production of ethanol and methane. Three major yeasts were compared for ethanol production in batch fermentations using CPW pretreated through acid hydrolysis and a combination of acid and enzyme hydrolysis. The most efficient conditions for production of CPW-based hydrolyzates included processing at 116 °C for 10 min. Pichia kudriavzevii KVMP10 achieved the highest ethanol production that reached 30.7 g L-1 in fermentations conducted at elevated temperatures (42 °C). A zero-waste biorefinery was introduced by using solid biorefinery residues in repeated batch anaerobic digestion fermentations achieving methane formation of 342 mL gVS-1 (volatile solids). Methane production applying untreated and dried CPW reached a similar level (339-356 mL gVS-1) to the use of the side stream, demonstrating that the developed bioprocess constitutes an advanced alternative to energy intensive methods for biofuel production.
Maria Patsalou; Charis G. Samanides; Eleni Protopapa; Stella Stavrinou; Ioannis Vyrides; Michalis Koutinas. A Citrus Peel Waste Biorefinery for Ethanol and Methane Production. Molecules 2019, 24, 2451 .
AMA StyleMaria Patsalou, Charis G. Samanides, Eleni Protopapa, Stella Stavrinou, Ioannis Vyrides, Michalis Koutinas. A Citrus Peel Waste Biorefinery for Ethanol and Methane Production. Molecules. 2019; 24 (13):2451.
Chicago/Turabian StyleMaria Patsalou; Charis G. Samanides; Eleni Protopapa; Stella Stavrinou; Ioannis Vyrides; Michalis Koutinas. 2019. "A Citrus Peel Waste Biorefinery for Ethanol and Methane Production." Molecules 24, no. 13: 2451.
This work explores the potential use of biochar as a microbial cell carrier enhancing the efficiency of alcoholic fermentations. Olive kernels, vineyard prunings, sewage sludge and seagrass residues were applied as biowaste for biochar production through pyrolysis at two different temperatures (250 °C and 500 °C), while a commercial type of non-biomass char was also employed for benchmarking purposes. Apart from vineyard prunings pyrolyzed at 250 °C, all other carbonaceous materials presented crystalline phases including halite, calcite, sylvite and/or silicon. Moreover, increase in pyrolysis temperature enhanced biochar’s porosity and BET-specific surface area, which reached 41.7 m2 g−1 for VP-based biochar remaining at lower levels (0.15–5.3 m2 g−1) in other specimens tested. Elemental analysis demonstrated reduction in oxygen and increase in the carbon content of biochars produced at elevated temperatures, while biochar from seagrass included residues of chloride (0.3–5.14%). Three major yeasts were immobilized on materials exhibiting the highest surface areas and applied in repeated batch fermentations using Valencia orange peel hydrolyzates as feedstock. The biocatalysts developed using S. cerevisiae and K. marxianus immobilized on vineyard prunings-based biochar exhibited exceptional ethanol productivities as compared to the relevant literature, which reached 7.2 g L−1 h−1 and 7.3 g L−1 h−1 respectively. Although the aforementioned strains improved biofuel production by 36–52% compared to the conventional process, P. kudriavzevii KVMP10 was not efficient following immobilization on biochar. The approach constitutes an innovative method for bioenergy production, demonstrating a novel application of biochar in industrial biotechnology which incorporates important technological advances such as enhanced biofuel production and biomass recycling.
Maria Kyriakou; Vasiliki K. Chatziiona; Costas N. Costa; Michalis Kallis; Loukas Koutsokeras; Georgios Constantinides; Michalis Koutinas. Biowaste-based biochar: A new strategy for fermentative bioethanol overproduction via whole-cell immobilization. Applied Energy 2019, 242, 480 -491.
AMA StyleMaria Kyriakou, Vasiliki K. Chatziiona, Costas N. Costa, Michalis Kallis, Loukas Koutsokeras, Georgios Constantinides, Michalis Koutinas. Biowaste-based biochar: A new strategy for fermentative bioethanol overproduction via whole-cell immobilization. Applied Energy. 2019; 242 ():480-491.
Chicago/Turabian StyleMaria Kyriakou; Vasiliki K. Chatziiona; Costas N. Costa; Michalis Kallis; Loukas Koutsokeras; Georgios Constantinides; Michalis Koutinas. 2019. "Biowaste-based biochar: A new strategy for fermentative bioethanol overproduction via whole-cell immobilization." Applied Energy 242, no. : 480-491.
Ionic liquids (ILs) have been characterized as contaminants of emerging concern (CEC) that often resist biodegradation and impose toxicity upon environmental release. Sphingomonas sp. MKIV has been isolated as an extreme microorganism capable for biodegradation of major classes of ILs. Six imidazolium-, pyridinium- and ammonium-based ILs (pyridinium trifluoromethanesulfonate [Py][CF3SO3], 1-(4-pyridyl)pyridinium chloride [1-4PPy][Cl], 1-butyl-3-methylimidazolium bromide [BMIM][Br], 1-butyl-3-methylimidazolium methanesulfonate [BMIM][MeSO4], tetrabutylammonium iodide [n-Bu4N][I] and tetrabutylammonium hexafluorophosphate [n-Bu4N][PF6]) were used for microbial growth. The strain achieved 91% and 87% removal efficiency for cultures supplemented with 100 mg L-1 of [BMIM][MeSO4] and [n-Bu4N][I] respectively. The metabolic activity of MKIV was inhibited following preliminary stages of cultures conducted using [BMIM][MeSO4], [BMIM][Br], [Py][CF3SO3] and [n-Bu4N][PF6], indicating potential accumulation of inhibitory metabolites. Thus, a comprehensive toxicological study of the six ILs on Aliivibrio fischeri, Daphnia magna and Raphidocelis subcapitata was conducted demonstrating that the compounds impose moderate and low toxicity. The end-products from [BMIM][MeSO4] and [n-Bu4N][I] biodegradation were assessed using Aliivibrio fischeri, exhibiting increased environmental impact of the latter following biotreatment. MKIV produced 19.29 g L-1 of biopolymer, comprising mainly glucose and galacturonic acid, from 25 g L-1 of glucose indicating high industrial significance for bioremediation and exopolysaccharide production.
Michalis Koutinas; Marlen I. Vasquez; Euthimia Nicolaou; Petros Pashali; Eleni Kyriakou; Elena Loizou; Aikaterini Papadaki; Apostolis A. Koutinas; Ioannis Vyrides. Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing Sphingomonas sp. for ionic liquids bioremediation. Journal of Hazardous Materials 2018, 365, 88 -96.
AMA StyleMichalis Koutinas, Marlen I. Vasquez, Euthimia Nicolaou, Petros Pashali, Eleni Kyriakou, Elena Loizou, Aikaterini Papadaki, Apostolis A. Koutinas, Ioannis Vyrides. Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing Sphingomonas sp. for ionic liquids bioremediation. Journal of Hazardous Materials. 2018; 365 ():88-96.
Chicago/Turabian StyleMichalis Koutinas; Marlen I. Vasquez; Euthimia Nicolaou; Petros Pashali; Eleni Kyriakou; Elena Loizou; Aikaterini Papadaki; Apostolis A. Koutinas; Ioannis Vyrides. 2018. "Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing Sphingomonas sp. for ionic liquids bioremediation." Journal of Hazardous Materials 365, no. : 88-96.
Currently, design and optimisation of biotechnological bioprocesses is performed either through exhaustive experimentation and/or with the use of empirical, unstructured growth kinetics models. Whereas, elaborate systems biology approaches have been recently explored, mixed-substrate utilisation is predominantly ignored despite its significance in enhancing bioprocess performance. Herein, bioprocess optimisation for an industrially-relevant bioremediation process involving a mixture of highly toxic substrates, m-xylene and toluene, was achieved through application of a novel experimental-modelling gene regulatory network – growth kinetic (GRN-GK) hybrid framework. The GRN model described the TOL and ortho-cleavage pathways in Pseudomonas putida mt-2 and captured the transcriptional kinetics expression patterns of the promoters. The GRN model informed the formulation of the growth kinetics model replacing the empirical and unstructured Monod kinetics. The GRN-GK framework's predictive capability and potential as a systematic optimal bioprocess design tool, was demonstrated by effectively predicting bioprocess performance, which was in agreement with experimental values, when compared to four commonly used models that deviated significantly from the experimental values. Significantly, a fed-batch biodegradation process was designed and optimised through the model-based control of TOL Pr promoter expression resulting in 61% and 60% enhanced pollutant removal and biomass formation, respectively, compared to the batch process. This provides strong evidence of model-based bioprocess optimisation at the gene level, rendering the GRN-GK framework as a novel and applicable approach to optimal bioprocess design. Finally, model analysis using global sensitivity analysis (GSA) suggests an alternative, systematic approach for model-driven strain modification for synthetic biology and metabolic engineering applications.
Argyro Tsipa; Michalis Koutinas; Chonlatep Usaku; Athanasios Mantalaris. Optimal bioprocess design through a gene regulatory network – Growth kinetic hybrid model: Towards replacing Monod kinetics. Metabolic Engineering 2018, 48, 129 -137.
AMA StyleArgyro Tsipa, Michalis Koutinas, Chonlatep Usaku, Athanasios Mantalaris. Optimal bioprocess design through a gene regulatory network – Growth kinetic hybrid model: Towards replacing Monod kinetics. Metabolic Engineering. 2018; 48 ():129-137.
Chicago/Turabian StyleArgyro Tsipa; Michalis Koutinas; Chonlatep Usaku; Athanasios Mantalaris. 2018. "Optimal bioprocess design through a gene regulatory network – Growth kinetic hybrid model: Towards replacing Monod kinetics." Metabolic Engineering 48, no. : 129-137.
This work explores the potential for development of a lupanine valorization process evaluating different isolated microorganisms for their capacity to metabolize the alkaloid. Ecotoxicological assessment demonstrated that lupanine is toxic for Vibrio fischeri and Daphnia magna exhibiting EC values of 89 mg L and 47 mg L respectively, while acting both as growth inhibitor for a monocotyledonous and as promoter for a dicotyledonous plant. Among the eight aerobic and anaerobic strains isolated and identified Rhodococcus rhodochrous LPK211 achieved 81% removal for 1.5 g L lupanine, while no end-products were detected by NMR constituting a promising microorganism for lupanine biodegradation. Moreover, Rhodococcus ruber LPK111 and Rhodococcus sp. LPK311 exhibited 66% and 71% of removal respectively, including potential formation of lupanine N-oxide. Pseudomonas putida LPK411 reached 80% of lupanine removal and generated three fermentation products potentially comprising 17-oxolupanine and lupanine derivatives with open ring structures enabling the development of alkaloid valorization processes.
Stella Parmaki; Ioannis Vyrides; Marlen I. Vasquez; Viki Hartman; Irene Zacharia; Ioanna Hadjiadamou; Catarina B.M. Barbeitos; Frederico Castelo Ferreira; Carlos Alberto Mateus Afonso; Chryssoula Drouza; Michalis Koutinas. Bioconversion of alkaloids to high-value chemicals: Comparative analysis of newly isolated lupanine degrading strains. Chemosphere 2018, 193, 50 -59.
AMA StyleStella Parmaki, Ioannis Vyrides, Marlen I. Vasquez, Viki Hartman, Irene Zacharia, Ioanna Hadjiadamou, Catarina B.M. Barbeitos, Frederico Castelo Ferreira, Carlos Alberto Mateus Afonso, Chryssoula Drouza, Michalis Koutinas. Bioconversion of alkaloids to high-value chemicals: Comparative analysis of newly isolated lupanine degrading strains. Chemosphere. 2018; 193 ():50-59.
Chicago/Turabian StyleStella Parmaki; Ioannis Vyrides; Marlen I. Vasquez; Viki Hartman; Irene Zacharia; Ioanna Hadjiadamou; Catarina B.M. Barbeitos; Frederico Castelo Ferreira; Carlos Alberto Mateus Afonso; Chryssoula Drouza; Michalis Koutinas. 2018. "Bioconversion of alkaloids to high-value chemicals: Comparative analysis of newly isolated lupanine degrading strains." Chemosphere 193, no. : 50-59.
This study explores the potential for enhancing the production of ethyl lactate (EL), a green solvent, through enzymatic esterification. Different solvents were compared as organic media for conversion of lactate and ethanol into EL, catalyzed by Novozym 435. Chloroform and hexane were the most effective in low acid concentrations (0.01-0.1M) exhibiting maximum EL yields of 88% and 75% respectively. The yield of EL improved as the solvent's LogP increased up to a value of 2. Non-commercial immobilized biocatalysts consisting heterologous Rhizopous oryzae (rROL) and Candida rugosa (CRL) lipases immobilized on hydrophobic supports were compared to commercial biocatalysts clarifying that Novozym 435 and Lipozyme RM IM could be efficiently applied. Operational stability tests were conducted using Novozym 435, which retained higher activity in chloroform as compared to hexane. Although non-commercial biocatalysts were not competitive in esterification, they exhibited significant activity towards hydrolysis constituting a valuable alternative to higher-cost options.
Michalis Koutinas; Chrystalleni Yiangou; Natália Osório; Katerina Ioannou; Albert Canet; Francisco Valero; Suzana Ferreira-Dias. Application of commercial and non-commercial immobilized lipases for biocatalytic production of ethyl lactate in organic solvents. Bioresource Technology 2018, 247, 496 -503.
AMA StyleMichalis Koutinas, Chrystalleni Yiangou, Natália Osório, Katerina Ioannou, Albert Canet, Francisco Valero, Suzana Ferreira-Dias. Application of commercial and non-commercial immobilized lipases for biocatalytic production of ethyl lactate in organic solvents. Bioresource Technology. 2018; 247 ():496-503.
Chicago/Turabian StyleMichalis Koutinas; Chrystalleni Yiangou; Natália Osório; Katerina Ioannou; Albert Canet; Francisco Valero; Suzana Ferreira-Dias. 2018. "Application of commercial and non-commercial immobilized lipases for biocatalytic production of ethyl lactate in organic solvents." Bioresource Technology 247, no. : 496-503.
Fumaric acid is a platform chemical with many applications in bio-based chemical and polymer production. Fungal cell morphology is an important factor that affects fumaric acid production via fermentation. In the present study, pellet and dispersed mycelia morphology of Rhizopus arrhizus NRRL 2582 was analysed using image analysis software and the impact on fumaric acid production was evaluated. Batch experiments were carried out in shake flasks using glucose as carbon source. The highest fumaric acid yield of 0.84 g/g total sugars was achieved in the case of dispersed mycelia with a final fumaric acid concentration of 19.7 g/L. The fumaric acid production was also evaluated using a nutrient rich feedstock obtained from soybean cake, as substitute of the commercial nitrogen sources. Solid state fermentation was performed in order to produce proteolytic enzymes, which were utilised for soybean cake hydrolysis. Batch fermentations were conducted using 50 g/L glucose and soybean cake hydrolysate achieving up to 33 g/L fumaric acid concentration. To the best of our knowledge the influence of R. arrhizus morphology on fumaric acid production has not been reported previously. The results indicated that dispersed clumps were more effective in fumaric acid production than pellets and renewable resources could be alternatively valorised for the biotechnological production of platform chemicals.
Aikaterini Papadaki; Nikolaos Androutsopoulos; Maria Patsalou; Michalis Koutinas; Nikolaos Kopsahelis; Aline Machado De Castro; Seraphim Papanikolaou; Apostolis A. Koutinas. Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582. Fermentation 2017, 3, 33 .
AMA StyleAikaterini Papadaki, Nikolaos Androutsopoulos, Maria Patsalou, Michalis Koutinas, Nikolaos Kopsahelis, Aline Machado De Castro, Seraphim Papanikolaou, Apostolis A. Koutinas. Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582. Fermentation. 2017; 3 (3):33.
Chicago/Turabian StyleAikaterini Papadaki; Nikolaos Androutsopoulos; Maria Patsalou; Michalis Koutinas; Nikolaos Kopsahelis; Aline Machado De Castro; Seraphim Papanikolaou; Apostolis A. Koutinas. 2017. "Biotechnological Production of Fumaric Acid: The Effect of Morphology of Rhizopus arrhizus NRRL 2582." Fermentation 3, no. 3: 33.
Toluene is a pollutant catabolised through the interconnected pWW0 (TOL) and ortho-cleavage pathways of Pseudomonas putida mt-2, while upon succinate and toluene mixtures introduction in batch cultures grown on M9 medium, succinate was previously reported as non-repressing. The effect of a 40 times lower succinate concentration, as compared to literature values, was explored through systematic real-time qPCR monitoring of transcriptional kinetics of the key TOL Pu, Pm and ortho-cleavage PbenR, PbenA promoters in mixed-substrate experiments. Even succinate trace inhibited transcription leading to bi-modal promoters expression. Potential carbon catabolite repression mechanisms and novel expression patterns of promoters were unfolded. Lag phase was shortened and biomass growth levels increased compared to sole toluene biodegradation suggesting enhanced pollutant removal efficiency. The study stressed the noticeable effect of a preferred compound's left-over on the main route of a bioprocess, revealing the beneficiary supply of low preferred substrates concentrations to design optimal bioremediation strategies.
Argyro Tsipa; Michalis Koutinas; Spyros I. Vernardis; Athanasios Mantalaris. The impact of succinate trace on pWW0 and ortho -cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation. Bioresource Technology 2017, 234, 397 -405.
AMA StyleArgyro Tsipa, Michalis Koutinas, Spyros I. Vernardis, Athanasios Mantalaris. The impact of succinate trace on pWW0 and ortho -cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation. Bioresource Technology. 2017; 234 ():397-405.
Chicago/Turabian StyleArgyro Tsipa; Michalis Koutinas; Spyros I. Vernardis; Athanasios Mantalaris. 2017. "The impact of succinate trace on pWW0 and ortho -cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation." Bioresource Technology 234, no. : 397-405.
Chrysanthi Pateraki; Maria Patsalou; Anestis Vlysidis; Nikolaos Kopsahelis; Colin Webb; Apostolis A. Koutinas; Michalis Koutinas. Actinobacillus succinogenes : Advances on succinic acid production and prospects for development of integrated biorefineries. Biochemical Engineering Journal 2016, 112, 285 -303.
AMA StyleChrysanthi Pateraki, Maria Patsalou, Anestis Vlysidis, Nikolaos Kopsahelis, Colin Webb, Apostolis A. Koutinas, Michalis Koutinas. Actinobacillus succinogenes : Advances on succinic acid production and prospects for development of integrated biorefineries. Biochemical Engineering Journal. 2016; 112 ():285-303.
Chicago/Turabian StyleChrysanthi Pateraki; Maria Patsalou; Anestis Vlysidis; Nikolaos Kopsahelis; Colin Webb; Apostolis A. Koutinas; Michalis Koutinas. 2016. "Actinobacillus succinogenes : Advances on succinic acid production and prospects for development of integrated biorefineries." Biochemical Engineering Journal 112, no. : 285-303.
This work explores the potential for the development of orange peel based ethanol bioprocesses through isolation of the thermotolerant Pichia kudriavzevii KVMP10. A model solution of hydrolysed Valencia orange peel was employed to determine the ethanologenic potential of the yeast, which was maximized at 42°C producing 54 g l−1 of ethanol. The effect of orange peel oil on bioethanol formation was investigated at 30 and 42°C confirming that the minimum inhibitory peel oil content was 0·01% (v/v). Pichia kudriavzevii KVMP10 demonstrated significant technological advantages for the production of sustainable bioenergy, such as utilization of both hexoses (glucose, sucrose, fructose and galactose) and pentoses (xylose) at high temperatures, exemplifying its great potential for application in orange peel based biorefineries for ethanol production. Citrus peel waste is one of the most underutilized and geographically diverse residues in the planet. In attempt to develop a citrus peel based biorefinery we report here the isolation of a yeast which exhibited favourable technological characteristics for the production of ethanol through utilization of the specific food waste. Pichia kudriavzevii KVMP10 was highly thermotolerant and utilized both hexoses and pentoses for ethanol production, which was achieved at elevated rates, highlighting its great potential for application in ethanol production processes from citrus peel.
M. Koutinas; M. Patsalou; S. Stavrinou; Ioannis Vyrides. High temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant Pichia kudriavzevii KVMP10. Letters in Applied Microbiology 2015, 62, 75 -83.
AMA StyleM. Koutinas, M. Patsalou, S. Stavrinou, Ioannis Vyrides. High temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant Pichia kudriavzevii KVMP10. Letters in Applied Microbiology. 2015; 62 (1):75-83.
Chicago/Turabian StyleM. Koutinas; M. Patsalou; S. Stavrinou; Ioannis Vyrides. 2015. "High temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant Pichia kudriavzevii KVMP10." Letters in Applied Microbiology 62, no. 1: 75-83.
Vanillin is a high value added product with many applications in the food, fragrance and pharmaceutical industries. A natural and low-cost method to produce vanillin is by microbial bioconversions through ferulic acid. Until now, limited microorganisms have been found capable of bioconverting ferulic acid to vanillin at high yield. This study aimed to screen halotolerant strains of bacteria from Larnaca Salt Lake which generate vanillin and vanillic acid from ferulic acid. From a total of 50 halotolenant/halophilic strains 8 grew in 1 g/L ferulic acid and only 1 Halomonas sp. B15 and 3 Halomonas elognata strains were capable of bioconverting ferulic acid to vanillic acid at 100 g NaCl/L. The highest vanillic acid (365 mg/L) at these conditions generated by Halomonas sp. B15 which corresponds to ferulic acid bioconversion yield of 36.5 %. Using the resting cell technique with an initial ferulic acid concentration of 0.5 g/L at low salinity, the highest production of vanillin (245 mg/L) took place after 48 h, corresponding to a bioconversion yield of 49 %. This is the first reported Halomonas sp. with high yield of vanillin production from ferulic acid at low salinity.
Ioannis Vyrides; Maria Agathangelou; Rodothea Dimitriou; Konstantinos Souroullas; Anastasia Salamex; Aristostodimos Ioannou; Michalis Koutinas. Novel Halomonas sp. B15 isolated from Larnaca Salt Lake in Cyprus that generates vanillin and vanillic acid from ferulic acid. World Journal of Microbiology and Biotechnology 2015, 31, 1291 -1296.
AMA StyleIoannis Vyrides, Maria Agathangelou, Rodothea Dimitriou, Konstantinos Souroullas, Anastasia Salamex, Aristostodimos Ioannou, Michalis Koutinas. Novel Halomonas sp. B15 isolated from Larnaca Salt Lake in Cyprus that generates vanillin and vanillic acid from ferulic acid. World Journal of Microbiology and Biotechnology. 2015; 31 (8):1291-1296.
Chicago/Turabian StyleIoannis Vyrides; Maria Agathangelou; Rodothea Dimitriou; Konstantinos Souroullas; Anastasia Salamex; Aristostodimos Ioannou; Michalis Koutinas. 2015. "Novel Halomonas sp. B15 isolated from Larnaca Salt Lake in Cyprus that generates vanillin and vanillic acid from ferulic acid." World Journal of Microbiology and Biotechnology 31, no. 8: 1291-1296.
Efi-Maria Drakou; Michalis Koutinas; Iakovos Pantelides; Maria-Dimitra Tsolakidou; Ioannis Vyrides. Insights into the metabolic basis of the halotolerant Pseudomonas aeruginosa strain LVD-10 during toluene biodegradation. International Biodeterioration & Biodegradation 2015, 99, 85 -94.
AMA StyleEfi-Maria Drakou, Michalis Koutinas, Iakovos Pantelides, Maria-Dimitra Tsolakidou, Ioannis Vyrides. Insights into the metabolic basis of the halotolerant Pseudomonas aeruginosa strain LVD-10 during toluene biodegradation. International Biodeterioration & Biodegradation. 2015; 99 ():85-94.
Chicago/Turabian StyleEfi-Maria Drakou; Michalis Koutinas; Iakovos Pantelides; Maria-Dimitra Tsolakidou; Ioannis Vyrides. 2015. "Insights into the metabolic basis of the halotolerant Pseudomonas aeruginosa strain LVD-10 during toluene biodegradation." International Biodeterioration & Biodegradation 99, no. : 85-94.
The bioeconomy era will rely on efficient fractionation of renewable resources via integrated biorefineries. The food supply chain waste, despite its inherent variability, could evolve into an important industrial feedstock on account of its availability, versatility, and sustainability, for the production of bio-based products. Waste streams generated from all stages of the life cycle of food products could be refined into different fractions, which will be either purified to high-value molecules or converted via green chemical and/or biotechnological routes for the production of bio-based products. A working group of the EUBis COST Action TD1203 is taking steps to gather a critical mass of knowledge and expertise to create innovation and technological breakthroughs.
Xavier Turon; Joachim Venus; Mehrdad Arshadi; Michalis Koutinas; Carol Sze Ki Lin; Apostolis Koutinas. Food Waste and Byproduct Valorization through Bio-processing: Opportunities and Challenges. BioResources 2014, 9, 5774-5777 .
AMA StyleXavier Turon, Joachim Venus, Mehrdad Arshadi, Michalis Koutinas, Carol Sze Ki Lin, Apostolis Koutinas. Food Waste and Byproduct Valorization through Bio-processing: Opportunities and Challenges. BioResources. 2014; 9 (4):5774-5777.
Chicago/Turabian StyleXavier Turon; Joachim Venus; Mehrdad Arshadi; Michalis Koutinas; Carol Sze Ki Lin; Apostolis Koutinas. 2014. "Food Waste and Byproduct Valorization through Bio-processing: Opportunities and Challenges." BioResources 9, no. 4: 5774-5777.
This work explores the potential for the development of a hybrid fermentation-enzymatic process for the production of ethyl lactate from dairy waste. Cheese whey was used in Kluyveromyces marxianus and Lactobacillus bulgaricus batch cultures to produce ethanol and lactic acid respectively. Subsequently, the fermentation products were transferred into an organic phase through liquid-liquid extraction and ethyl lactate was formed in an esterification reaction catalyzed by lipases. The production of ethanol and lactic acid achieved under different conditions was 23gL(-1) and 29gL(-1), respectively. Furthermore, the efficiency of various organic solvents for the esterification reaction was evaluated and toluene was chosen for application in the process. The effect of water content was determined aiming to maximize the product yield and 40mgml(-1) was the optimal enzyme concentration. The bioprocess achieved maximum conversion of 33% constituting a valuable alternative to the application of energy demanding chemically derived methods.
Michalis Koutinas; Maria Menelaou; Evrydiki N. Nicolaou. Development of a hybrid fermentation–enzymatic bioprocess for the production of ethyl lactate from dairy waste. Bioresource Technology 2014, 165, 343 -349.
AMA StyleMichalis Koutinas, Maria Menelaou, Evrydiki N. Nicolaou. Development of a hybrid fermentation–enzymatic bioprocess for the production of ethyl lactate from dairy waste. Bioresource Technology. 2014; 165 ():343-349.
Chicago/Turabian StyleMichalis Koutinas; Maria Menelaou; Evrydiki N. Nicolaou. 2014. "Development of a hybrid fermentation–enzymatic bioprocess for the production of ethyl lactate from dairy waste." Bioresource Technology 165, no. : 343-349.
This chapter contains sections titled: Introduction Review of Mathematical Models of Mammalian Cell Culture Systems Motivation Dynamic Modeling of Biological Systems – An Illustrative Example Concluding Remarks
Alexandros Kiparissides; Michalis Koutinas; Efstratios N. Pistikopoulos; Athanasios Mantalaris. Model Development and Analysis of Mammalian Cell Culture Systems. Process Systems Engineering 2014, 403 -439.
AMA StyleAlexandros Kiparissides, Michalis Koutinas, Efstratios N. Pistikopoulos, Athanasios Mantalaris. Model Development and Analysis of Mammalian Cell Culture Systems. Process Systems Engineering. 2014; ():403-439.
Chicago/Turabian StyleAlexandros Kiparissides; Michalis Koutinas; Efstratios N. Pistikopoulos; Athanasios Mantalaris. 2014. "Model Development and Analysis of Mammalian Cell Culture Systems." Process Systems Engineering , no. : 403-439.