Cellulose deposition in developing cotton fibers has been studied previously with analytical techniques, such as Fourier transform infrared spectroscopy (FTIR), High-performance liquid chromatography (HPLC) and Thermogravimetric analysis (TGA). Recent technological developments in instrumentation have made Raman microscopy emerge as an extraordinary analytical tool in biological and plant research. The advantage of using confocal Raman microscopy (CRM) resides in the lateral spatial resolution and in the fact that Raman spectroscopy provides not only chemical composition information, but also structural information. Cross-sections of cotton fibers harvested at different developmental stages were studied with CRM. The Raman bands assigned to cellulose were analyzed. The results of this study indicate that CRM can be used as a tool to study cellulose deposition in cotton fibers and could provide useful information on cellulose deposition during cotton fiber development.

While oilfield produced water (PW) is one of the largest, unclaimed wastewater streams of the oil industry, it could potentially be used as a cultivation medium for microalgae. Microalgae could help with the remediation of this water while also delivering biomass that can be transformed into valuable byproducts such as biofuels. The coupling of these two purposes is expected to cut production costs of biofuels while aiding environmental protection. In this study, we compared the cultivation capacity of the marine model diatom Phaeodactylum tricornutum in media at varying salinities and in media composed of PW from two oilfields in the Central Valley of California that differed drastically in the concentration of inorganic and organic constituents. Specifically, we measured the carrying capacity of these media, the maximum growth rates of P. tricornutum, its cellular lipid accumulation capacity, and its capacity to remediate the most polluted PW source. Our study shows that P. tricornutum can successfully adjust to the tested cultivation media through processes of short-term acclimation and long-term adaptation. Furthermore, the cultivation of P. tricornutum in the most heavily polluted PW source led to significant increases in cell yield and improved photosynthetic capacity during the stationary phase, which could be attributed chiefly to the higher levels of nitrate present in this PW source. Chemical water analyses also demonstrated the capability of P. tricornutum to remediate major nutrient content and potentially harmful elements like fluorine and copper. Because P. tricornutum is amenable to advanced genetic engineering, which could be taken advantage of to improve its cultivation resilience and productivity in an economic setting, we propose this study as a step towards essential follow-up studies that will identify the genetic regulation behind its growth in oilfield PW media and its remediation of the PW constituents.

During cotton fibers development, important structural changes occur, which lead to cellulose deposition and organization in the secondary cell wall. Several studies have focused on the analysis of the cell wall extracts of cotton fibers to gain an understanding of the changes in carbohydrate profiles and to determine the changes in crystallinity, cellulosic and non-cellulosic compounds at various stages of the fiber cell wall development. In this research, thermogravimetric analysis (TGA) was used to study intact fibers harvested from two cotton genotypes. Cellulose macromolecules structural changes occurring during different developmental stages were studied. The results from TGA technique were in agreement with results from other analytical techniques, which indicates that TGA could be a great tool to investigate the onset of cellulose deposition and to evaluate the cell wall composition during fiber development. The results obtained in this study demonstrated that the initiation of the secondary cell wall is genotype-dependent.

Oils and fats derived from animals and plants provide a good renewable source for polymer precursors. In this investigation, fatty acids derived from plant oils and diols were used as monomers to produce polyesters by melt polycondensation. Sebacic acid, hexanediol, and hydroquinone were used as precursors in the polymer synthesis. The polymers were characterized by gel permeation chromatography, Fourier transform infrared spectroscopy, three-bending point flexural test, X-ray diffraction, tensile testing, and contact angle. The resulting polyesters were blended with epoxies to create materials with an increased elongation at break without affecting other mechanical properties.

Increased understanding of demand for transport energy and how to improve road pavement materials would enable decision makers to make environmental, financial, and other positive changes in future planning and design of roads, bridges, and other important transportation structures. This research comprises three studies focused on pavement materials and a fourth study that examines energy demand within the road transportation sector. These studies are as follows: 1. A techno-economic study of ground tire rubber as an asphalt modifier; 2. A computational fluid dynamics analysis comparing the urban heat island effect of two different pavement materials – asphalt and Portland Cement Concrete; 3. A new approach that modifies the surface of ground tire rubber using low-cost chemicals and treatment methods to be used in asphalt applications; and 4. Analysis of road transport energy demand in California and the United States. The findings of these studies include that 1. GTR is an effective and economically suitable additive for modified asphalt, 2. the suitability of PCC pavements in urban settings should be reexamined, 3. Surface modification of GTR materials can improve compatibilization of particles for the manufacture of asphalt materials, and 4. gasoline sales are generally price inelastic in both the U.S. and California. Ultimately, these four studies improve understanding of road pavement materials and transport energy demand. They lay out important information about the future of the relationship between materials and design in the transportation industry. These findings may be used by engineers, policymakers, and others in the industry to better consider implications of decisions involved in design, creation, and modification of structures using pavement and concrete, including roads, bridges, etc.