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Prof. norihiro nishimura
Mie University, Regional Innovation Studies

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Journal article
Published: 30 April 2021 in Molecules
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Various natural products (NPs) have been used to treat obesity and related diseases. However, the best way to fight obesity is preventive, with accurate body weight management through exercise, diet, or bioactive NPs to avoid obesity development. We demonstrated that green tea extract (GTE) is an anti-obesity NP using a zebrafish obesity model. Based on a hypothesis that GTE can prevent obesity, the objective of this study was to assess GTE’s ability to attenuate obesity development. Juvenile zebrafish were pretreated with GTE for seven days before obesity induction via a high-fat diet; adult zebrafish were pretreated with GTE for two weeks before obesity induction by overfeeding. As a preventive intervention, GTE significantly decreased visceral adipose tissue accumulation in juveniles and ameliorated visceral adiposity and plasma triglyceride levels in adult zebrafish obesity models. RNA sequencing analysis was performed using liver tissues from adult obese zebrafish, with or without GTE administration, to investigate the underlying molecular mechanism. Transcriptome analysis revealed that preventive GTE treatment affects several pathways associated with anti-obesity regulation, including activation of STAT and downregulation of CEBP signaling pathways. In conclusion, GTE could be used as a preventive agent against obesity.

ACS Style

Liqing Zang; Yasuhito Shimada; Hiroko Nakayama; Hirotaka Katsuzaki; Youngil Kim; Djong-Chi Chu; Lekh Juneja; Junya Kuroyanagi; Norihiro Nishimura. Preventive Effects of Green Tea Extract against Obesity Development in Zebrafish. Molecules 2021, 26, 2627 .

AMA Style

Liqing Zang, Yasuhito Shimada, Hiroko Nakayama, Hirotaka Katsuzaki, Youngil Kim, Djong-Chi Chu, Lekh Juneja, Junya Kuroyanagi, Norihiro Nishimura. Preventive Effects of Green Tea Extract against Obesity Development in Zebrafish. Molecules. 2021; 26 (9):2627.

Chicago/Turabian Style

Liqing Zang; Yasuhito Shimada; Hiroko Nakayama; Hirotaka Katsuzaki; Youngil Kim; Djong-Chi Chu; Lekh Juneja; Junya Kuroyanagi; Norihiro Nishimura. 2021. "Preventive Effects of Green Tea Extract against Obesity Development in Zebrafish." Molecules 26, no. 9: 2627.

Journal article
Published: 17 January 2021 in Sustainability
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Online education in China is developing at a rapid pace due to its unique advantages, and its sustainable development is becoming increasingly crucial. Thus, this study attempted to understand learners’ continuance intentions in an online learning environment and examined the factors influencing online learners’ continuous retention. The research model for the influencing factors and study hypotheses were constructed based on multiple theoretical and synthesized perspectives, such as the information system success model; interactions between students, content, and instructors; and the theory of perceived value. To achieve the stated objectives, we conducted a questionnaire survey, in which 382 valid responses were collected from Chinese respondents from 32 provinces in China in April and May 2020. Furthermore, this study primarily employed Structural Equation Modeling (SEM) and Partial Least Squares Structural Equation Modeling (PLS-SEM) to test the constructed model. The results indicate that service quality, course quality, and student–instructor interaction have indirect and positive effects on learners’ continuance intentions for online learning, while the variable of perceived value is a significant mediator for online learners’ retention and has a direct influence on their continuance intentions. Student–student interaction and student–content interaction do not have direct or indirect effects on online learners’ continuance intentions.

ACS Style

Yiwen Li; Norihiro Nishimura; Hisanori Yagami; Hye-Sook Park. An Empirical Study on Online Learners’ Continuance Intentions in China. Sustainability 2021, 13, 889 .

AMA Style

Yiwen Li, Norihiro Nishimura, Hisanori Yagami, Hye-Sook Park. An Empirical Study on Online Learners’ Continuance Intentions in China. Sustainability. 2021; 13 (2):889.

Chicago/Turabian Style

Yiwen Li; Norihiro Nishimura; Hisanori Yagami; Hye-Sook Park. 2021. "An Empirical Study on Online Learners’ Continuance Intentions in China." Sustainability 13, no. 2: 889.

Journal article
Published: 10 December 2020 in Molecules
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(1) Background: The obesity epidemic has been drastically progressing in both children and adults worldwide. Pharmacotherapy is considered necessary for its treatment. However, many anti-obesity drugs have been withdrawn from the market due to their adverse effects. Instead, natural products (NPs) have been studied as a source for drug discovery for obesity, with the goal of limiting the adverse effects. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, and disease models of several types of obesity have been developed. However, the evidence for zebrafish as an anti-obesity drug screening model are still limited. (2) Methods: We performed anti-adipogenic testing using the juvenile zebrafish obesogenic test (ZOT) and mouse 3T3-L1 preadipocytes using the focused NP library containing 38 NPs and compared their results. (3) Results: Seven and eleven NPs reduced lipid accumulation in zebrafish visceral fat tissues and mouse adipocytes, respectively. Of these, five NPs suppressed lipid accumulation in both zebrafish and 3T3-L1 adipocytes. We confirmed that these five NPs (globin-digested peptides, green tea extract, red pepper extract, nobiletin, and Moringa leaf powder) exerted anti-obesity effects in diet-induced obese adult zebrafish. (4) Conclusions: ZOT using juvenile fish can be a high-throughput alternative to ZOT using adult zebrafish and can be applied for in vivo screening to discover novel therapeutics for visceral obesity and potentially also other disorders.

ACS Style

Hiroko Nakayama; Kanae Hata; Izumi Matsuoka; Liqing Zang; Youngil Kim; Djong-Chi Chu; Lekh Raj Juneja; Norihiro Nishimura; Yasuhito Shimada. Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays. Molecules 2020, 25, 5840 .

AMA Style

Hiroko Nakayama, Kanae Hata, Izumi Matsuoka, Liqing Zang, Youngil Kim, Djong-Chi Chu, Lekh Raj Juneja, Norihiro Nishimura, Yasuhito Shimada. Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays. Molecules. 2020; 25 (24):5840.

Chicago/Turabian Style

Hiroko Nakayama; Kanae Hata; Izumi Matsuoka; Liqing Zang; Youngil Kim; Djong-Chi Chu; Lekh Raj Juneja; Norihiro Nishimura; Yasuhito Shimada. 2020. "Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays." Molecules 25, no. 24: 5840.

Review
Published: 14 September 2020 in Chemosphere
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Azoles are considered as one of the most efficient fungicides for the treatment of humans, animals, and plant fungal pathogens. They are of significant clinical importance as antifungal drugs and are widely used in personal care products, ultraviolet stabilizers, and in aircraft for its anti-corrosive properties. The prevalence of azole compounds in the natural environment and its accumulation in fish raises questions about its impact on aquatic organisms. The objective of this paper is to review the scientific studies on the effects of azole compounds in fish and to discuss future opportunities for the risk evaluation. A systematic literature search was conducted on Web of Science, PubMed, and ScienceDirect to locate peer-reviewed scientific articles on occurrence, environmental fate, and toxicological impact of azole fungicides on fish. Studies included in this review provide ample evidence that azole compounds are not only commonly detected in the natural environment but also cause several detrimental effects on fish. Future studies with environmentally relevant concentrations of azole alone or in combination with other commonly occurring contaminants in a multigenerational study could provide a better understanding. Based on current knowledge and studies reporting adverse biological effects of azole on fish, considerable attention is required for better management and effective ecological risk assessment of these emerging contaminants.

ACS Style

Jacky Bhagat; Nisha Singh; Norihiro Nishimura; Yasuhito Shimada. A comprehensive review on environmental toxicity of azole compounds to fish. Chemosphere 2020, 262, 128335 .

AMA Style

Jacky Bhagat, Nisha Singh, Norihiro Nishimura, Yasuhito Shimada. A comprehensive review on environmental toxicity of azole compounds to fish. Chemosphere. 2020; 262 ():128335.

Chicago/Turabian Style

Jacky Bhagat; Nisha Singh; Norihiro Nishimura; Yasuhito Shimada. 2020. "A comprehensive review on environmental toxicity of azole compounds to fish." Chemosphere 262, no. : 128335.

Journal article
Published: 31 August 2020 in Molecules
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In recent decades, zebrafish (Danio rerio) has become a widely used vertebrate animal model for studying development and human diseases. However, studies on skin medication using zebrafish are rare. Here, we developed a novel protocol for percutaneous absorption of molecules via the zebrafish tail skin, by applying a liquid solution directly, or using a filter paper imbibed with a chemical solution (coating). Human skin is capable of absorbing felbinac and loxoprofen sodium hydrate (LSH), but not glycyrrhetinic acid (GA) and terbinafine hydrochloride (TH). To evaluate the possibility and the quality of transdermal absorption in zebrafish, we transdermally administered these four drugs to zebrafish. Pharmacokinetics showed that felbinac was present in the blood of zebrafish subjected to all administration methods. Felbinac blood concentrations peaked at 2 h and disappeared 7 h after administration. GA was not detected following transdermal administrations, but was following exposure. LSH was not found in the circulatory system after transdermal administration, but TH was. A dose-response correlation was observed for felbinac blood concentration. These findings suggest that zebrafish are capable of absorbing drug molecules through their skin. However, the present data cannot demonstrate that zebrafish is a practical model to predict human skin absorption. Further systemic studies are needed to observe the correlations in percutaneous absorption between humans and zebrafish.

ACS Style

Daizo Morikane; Liqing Zang; Norihiro Nishimura. Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish. Molecules 2020, 25, 3974 .

AMA Style

Daizo Morikane, Liqing Zang, Norihiro Nishimura. Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish. Molecules. 2020; 25 (17):3974.

Chicago/Turabian Style

Daizo Morikane; Liqing Zang; Norihiro Nishimura. 2020. "Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish." Molecules 25, no. 17: 3974.

Journal article
Published: 31 March 2020 in Molecules
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Ceramides have several well-known biological properties, including anti-pigmentation and anti-melanogenesis, which make them applicable for use in skincare products in cosmetics. However, the efficacy of ceramides is still limited. Dermal or transdermal drug delivery systems can enhance the anti-pigmentation properties of ceramides, although there is currently no systemic evaluation method for the efficacy of these systems. Here we prepared several types of lecithin-based emulsion of maize-derived glucosylceramide, determining PC70-ceramide (phosphatidylcholine-base) to be the safest and most effective anti-pigmentation agent using zebrafish larvae. We also demonstrated the efficacy of PC70 as a drug delivery system by showing that PC70-Nile Red (red fluorescence) promoted Nile Red accumulation in the larval bodies. In addition, PC70-ceramide suppressed melanin in mouse B16 melanoma cells compared to ceramide alone. In conclusion, we developed a lecithin-based dermal delivery method for ceramide using zebrafish larvae with implications for human clinical use.

ACS Style

Kazuhiro Kagotani; Hiroko Nakayama; Liqing Zang; Yuki Fujimoto; Akihito Hayashi; Ryoji Sono; Norihiro Nishimura; Yasuhito Shimada. Lecithin-Based Dermal Drug Delivery for Anti-Pigmentation Maize Ceramide. Molecules 2020, 25, 1595 .

AMA Style

Kazuhiro Kagotani, Hiroko Nakayama, Liqing Zang, Yuki Fujimoto, Akihito Hayashi, Ryoji Sono, Norihiro Nishimura, Yasuhito Shimada. Lecithin-Based Dermal Drug Delivery for Anti-Pigmentation Maize Ceramide. Molecules. 2020; 25 (7):1595.

Chicago/Turabian Style

Kazuhiro Kagotani; Hiroko Nakayama; Liqing Zang; Yuki Fujimoto; Akihito Hayashi; Ryoji Sono; Norihiro Nishimura; Yasuhito Shimada. 2020. "Lecithin-Based Dermal Drug Delivery for Anti-Pigmentation Maize Ceramide." Molecules 25, no. 7: 1595.

Journal article
Published: 06 September 2019 in Molecules
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Green tea is a popular beverage that is rich in polyphenolic compounds such as catechins. Its major content, (-)-epigallocatechin-3-gallate, has been shown to have beneficial effects on several diseases including cancer, metabolic syndrome, cardiovascular diseases, and neurodegenerative diseases. The aim of this study was to assess the anti-obesity effects and the underlying molecular mechanisms of green tea extract (GTE) using zebrafish larva and adult obesity models. We administered 100 μg/mL GTE to zebrafish larvae and performed a short-term obesogenic test. GTE significantly decreased the visceral adipose tissue volume induced by a high-fat diet. Oral administration (250 µg/g body weight/day) of GTE to adult diet-induced obese zebrafish also significantly reduced their visceral adipose tissue volume, with a reduction of plasma triglyceride and total cholesterol levels. To investigate the molecular mechanism underlying the GTE effects, we conducted RNA sequencing using liver tissues of adult zebrafish and found that GTE may ameliorate the obese phenotypes via the activation of Wnt/β-catenin and adenosine monophosphate-activated protein kinase (AMPK) pathway signaling. In addition, the comparative transcriptome analysis revealed that zebrafish and mammals may share a common molecular response to GTE. Our findings suggest that daily consumption of green tea may be beneficial for the prevention and treatment of obesity.

ACS Style

Liqing Zang; Yasuhito Shimada; Hiroko Nakayama; Youngil Kim; Djong-Chi Chu; Lekh Raj Juneja; Junya Kuroyanagi; Norihiro Nishimura; Zang; Kim; Chu. RNA-seq Based Transcriptome Analysis of the Anti-Obesity Effect of Green Tea Extract Using Zebrafish Obesity Models. Molecules 2019, 24, 3256 .

AMA Style

Liqing Zang, Yasuhito Shimada, Hiroko Nakayama, Youngil Kim, Djong-Chi Chu, Lekh Raj Juneja, Junya Kuroyanagi, Norihiro Nishimura, Zang, Kim, Chu. RNA-seq Based Transcriptome Analysis of the Anti-Obesity Effect of Green Tea Extract Using Zebrafish Obesity Models. Molecules. 2019; 24 (18):3256.

Chicago/Turabian Style

Liqing Zang; Yasuhito Shimada; Hiroko Nakayama; Youngil Kim; Djong-Chi Chu; Lekh Raj Juneja; Junya Kuroyanagi; Norihiro Nishimura; Zang; Kim; Chu. 2019. "RNA-seq Based Transcriptome Analysis of the Anti-Obesity Effect of Green Tea Extract Using Zebrafish Obesity Models." Molecules 24, no. 18: 3256.

Journal article
Published: 21 March 2019 in International Journal of Molecular Sciences
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Down and feather materials have been commonly used and promoted as natural stuffing for warm clothing and bedding. These materials tend to become more allergenic as they become contaminated with microorganisms, in addition to being subjected to several kinds of chemical treatments. The biological or chemical contaminants in these materials pose a major risk to human health, to consumers and manufacturers alike. Here, we report the development of an integrative evaluation method for down and feather materials to assess bacterial contamination and in vivo toxicity. To assess bacterial contamination, we quantified 16S ribosomal RNA, performed culture tests, and established a conversion formula. To determine in vivo toxicity, we performed a zebrafish embryo toxicity testing (ZFET). Washing the material appropriately decreases the actual number of bacteria in the down and feather samples; in addition, after washing, 16S rRNA sequencing revealed that the bacterial compositions were similar to those in rinse water. The ZFET results showed that even materials with low bacterial contamination showed high toxicity or high teratogenicity, probably because of the presence of unknown chemical additives. We established an integrative evaluation method for down and feather safety, based on bacterial contamination with in vivo toxicity testing.

ACS Style

Toshikatsu Kawada; Junya Kuroyanagi; Fumiyoshi Okazaki; Mizuki Taniguchi; Hiroko Nakayama; Narumi Suda; Souta Abiko; Satoshi Kaneco; Norihiro Nishimura; Yasuhito Shimada. An Integrative Evaluation Method for the Biological Safety of Down and Feather Materials. International Journal of Molecular Sciences 2019, 20, 1434 .

AMA Style

Toshikatsu Kawada, Junya Kuroyanagi, Fumiyoshi Okazaki, Mizuki Taniguchi, Hiroko Nakayama, Narumi Suda, Souta Abiko, Satoshi Kaneco, Norihiro Nishimura, Yasuhito Shimada. An Integrative Evaluation Method for the Biological Safety of Down and Feather Materials. International Journal of Molecular Sciences. 2019; 20 (6):1434.

Chicago/Turabian Style

Toshikatsu Kawada; Junya Kuroyanagi; Fumiyoshi Okazaki; Mizuki Taniguchi; Hiroko Nakayama; Narumi Suda; Souta Abiko; Satoshi Kaneco; Norihiro Nishimura; Yasuhito Shimada. 2019. "An Integrative Evaluation Method for the Biological Safety of Down and Feather Materials." International Journal of Molecular Sciences 20, no. 6: 1434.

Journal article
Published: 10 October 2018 in Analytical Sciences
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ACS Style

Zhen Chen; Liqing Zang; Yue Wu; Hiroko Nakayama; Yasuhito Shimada; Rojeet Shrestha; Yaoyao Zhao; Yusuke Miura; Hitoshi Chiba; Shu-Ping Hui; Norihiro Nishimura. Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish. Analytical Sciences 2018, 34, 1201 -1208.

AMA Style

Zhen Chen, Liqing Zang, Yue Wu, Hiroko Nakayama, Yasuhito Shimada, Rojeet Shrestha, Yaoyao Zhao, Yusuke Miura, Hitoshi Chiba, Shu-Ping Hui, Norihiro Nishimura. Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish. Analytical Sciences. 2018; 34 (10):1201-1208.

Chicago/Turabian Style

Zhen Chen; Liqing Zang; Yue Wu; Hiroko Nakayama; Yasuhito Shimada; Rojeet Shrestha; Yaoyao Zhao; Yusuke Miura; Hitoshi Chiba; Shu-Ping Hui; Norihiro Nishimura. 2018. "Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish." Analytical Sciences 34, no. 10: 1201-1208.

Journal article
Published: 02 October 2018 in Nutrients
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(1) Background: The red seaweed Palmaria mollis (PM), which has a bacon-like taste, is increasingly being included in Western diets. In this study, we evaluate anti-obesity effects of PM using diet-induced obese (DIO) zebrafish and mice models. (2) Methods: We fed PM-containing feed to DIO-zebrafish and mice, and evaluated the anti-obesity effects We also analyzed gene expression changes in their liver and visceral adipose tissues (VAT). (3) Results: PM ameliorated several anti-obesity traits in both animals, including dyslipidaemia, hepatic steatosis, and visceral adiposity. In liver tissues of DIO-zebrafish and mice, PM upregulated gene expressions involved in peroxisome proliferator-activated receptor alpha (PPARA) pathways, and downregulated peroxisome proliferator-activated receptor gamma (PPARG) pathways, suggesting that the lipid-lowering effect of PM might be caused by activation of beta-oxidation and inhibition of lipogenesis. In VAT, PM downregulated genes involved in early and late adipocyte differentiation in zebrafish, but not in mice. (4) Conclusions: We have demonstrated that PM can prevent hepatic steatosis and visceral adiposity for the first time. Dietary supplementation of PM as a functional food may be suitable for obesity prevention and reduction in the prevalence of obesity-related diseases.

ACS Style

Hiroko Nakayama; Yasuhito Shimada; Liqing Zang; Masahiro Terasawa; Kaoru Nishiura; Koichi Matsuda; Charles Toombs; Chris Langdon; Norihiro Nishimura. Novel Anti-Obesity Properties of Palmaria mollis in Zebrafish and Mouse Models. Nutrients 2018, 10, 1401 .

AMA Style

Hiroko Nakayama, Yasuhito Shimada, Liqing Zang, Masahiro Terasawa, Kaoru Nishiura, Koichi Matsuda, Charles Toombs, Chris Langdon, Norihiro Nishimura. Novel Anti-Obesity Properties of Palmaria mollis in Zebrafish and Mouse Models. Nutrients. 2018; 10 (10):1401.

Chicago/Turabian Style

Hiroko Nakayama; Yasuhito Shimada; Liqing Zang; Masahiro Terasawa; Kaoru Nishiura; Koichi Matsuda; Charles Toombs; Chris Langdon; Norihiro Nishimura. 2018. "Novel Anti-Obesity Properties of Palmaria mollis in Zebrafish and Mouse Models." Nutrients 10, no. 10: 1401.

Journal article
Published: 01 August 2015 in Journal of Functional Foods
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ACS Style

Liqing Zang; Yasuhito Shimada; Toshio Tanaka; Norihiro Nishimura. Rhamnan sulphate from Monostroma nitidum attenuates hepatic steatosis by suppressing lipogenesis in a diet-induced obesity zebrafish model. Journal of Functional Foods 2015, 17, 364 -370.

AMA Style

Liqing Zang, Yasuhito Shimada, Toshio Tanaka, Norihiro Nishimura. Rhamnan sulphate from Monostroma nitidum attenuates hepatic steatosis by suppressing lipogenesis in a diet-induced obesity zebrafish model. Journal of Functional Foods. 2015; 17 ():364-370.

Chicago/Turabian Style

Liqing Zang; Yasuhito Shimada; Toshio Tanaka; Norihiro Nishimura. 2015. "Rhamnan sulphate from Monostroma nitidum attenuates hepatic steatosis by suppressing lipogenesis in a diet-induced obesity zebrafish model." Journal of Functional Foods 17, no. : 364-370.

Comparative study
Published: 01 December 2011 in Zebrafish
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A novel protocol using gluten as a carrier material was developed to administer chemicals to adult zebrafish, per os (p.o.). To evaluate the capacity of gluten to retain chemicals, we prepared gluten granules containing eight types of chemicals with different Log Pow values and immersed them in water. Less than 5% of chemicals were eluted from gluten granules within 5 min, a standard feeding time for zebrafish. Although retention capability was dependent on the hydrophilicity and hydrophobicity of the chemicals, the gluten granules retained 62%–99% of the total amount of chemical, even after immersion in water for 60 min. Vital staining dyes, such as 4-Di-2-Asp and Nile red, administered p.o., were delivered into the gastrointestinal tract where they were digested and secreted. Subsequently, we conducted a pharmacokinetic study of oral administration of felbinac and confirmed that it was successfully delivered into the blood of zebrafish. This indicates that chemicals administered using gluten granules are satisfactorily absorbed from the digestive tract and delivered into the metabolic system. The absorption, distribution, and pharmacokinetics of chemicals given by oral administration were also compared with those of chemicals given by alternative administration routes such as intraperitoneal injection and exposure to chemical solution.

ACS Style

Liqing Zang; Daizo Morikane; Yasuhito Shimada; Toshio Tanaka; Norihiro Nishimura. A Novel Protocol for the Oral Administration of Test Chemicals to Adult Zebrafish. Zebrafish 2011, 8, 203 -210.

AMA Style

Liqing Zang, Daizo Morikane, Yasuhito Shimada, Toshio Tanaka, Norihiro Nishimura. A Novel Protocol for the Oral Administration of Test Chemicals to Adult Zebrafish. Zebrafish. 2011; 8 (4):203-210.

Chicago/Turabian Style

Liqing Zang; Daizo Morikane; Yasuhito Shimada; Toshio Tanaka; Norihiro Nishimura. 2011. "A Novel Protocol for the Oral Administration of Test Chemicals to Adult Zebrafish." Zebrafish 8, no. 4: 203-210.