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Lu Wang
School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China

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Journal article
Published: 24 June 2017 in AMB Express
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Monascus species can produce secondary metabolites that have a polyketide structure. In this study, four types of extracellular water-soluble yellow pigments (Y1-Y4) were generated by submerged fermentation with Monascus ruber CGMCC 10910, of which Y3 and Y4 had strong yellow fluorescence. The composition of the pigment mixtures was closely related to the fermentation temperature. The dominating pigments changed from Y1 to Y3 and Y4 when fermentation temperature increased from 30 to 35 °C. Increasing the temperature to 35 °C changed the metabolic pathways of the pigments, which inhibited the biosynthesis of Y1 and enhanced the biosynthesis of Y3 and Y4. Moreover, the yield of Y1 reduced insignificantly, while the yields of Y3 and Y4 increased by 98.21 and 79.31% respectively under two-stage temperature fermentation condition. The expression levels of the relative pigment biosynthetic genes, such as MpFasA2, MpFasB2, MpPKS5, mppR1, mppB, and mppE, were up-regulated at 35 °C. The two-stage temperature strategy is a potential method for producing water-soluble Monascus yellow pigments with strong yellow fluorescence.

ACS Style

Tao Huang; Hailing Tan; Gong Chen; Lu Wang; Zhenqiang Wu. Rising temperature stimulates the biosynthesis of water-soluble fluorescent yellow pigments and gene expression in Monascus ruber CGMCC10910. AMB Express 2017, 7, 134 .

AMA Style

Tao Huang, Hailing Tan, Gong Chen, Lu Wang, Zhenqiang Wu. Rising temperature stimulates the biosynthesis of water-soluble fluorescent yellow pigments and gene expression in Monascus ruber CGMCC10910. AMB Express. 2017; 7 (1):134.

Chicago/Turabian Style

Tao Huang; Hailing Tan; Gong Chen; Lu Wang; Zhenqiang Wu. 2017. "Rising temperature stimulates the biosynthesis of water-soluble fluorescent yellow pigments and gene expression in Monascus ruber CGMCC10910." AMB Express 7, no. 1: 134.

Journal article
Published: 02 February 2016 in Applied Microbiology and Biotechnology
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Citric acid is produced by an industrial-scale process of fermentation using Aspergillus niger as a microbial cell factory. However, citric acid production was hindered by the non-fermentable isomaltose and insufficient saccharification ability in A. niger when liquefied corn starch was used as a raw material. In this study, A. niger TNA 101ΔagdA was constructed by deletion of the α-glucosidase-encoding agdA gene in A. niger CGMCC 10142 genome using Agrobacterium tumefaciens-mediated transformation. The transformants A. niger OG 1, OG 17, and OG 31 then underwent overexpression of glucoamylase in A. niger TNA 101ΔagdA. The results showed that the α-glucosidase activity of TNA 101ΔagdA was decreased by 62.5 % compared with CGMCC 10142, and isomaltose was almost undetectable in the fermentation broth. The glucoamylase activity of the transformants OG 1 and OG 17 increased by 34.5 and 16.89 % compared with that of TNA 101ΔagdA, respectively. In addition, for the recombinants TNA 101ΔagdA, OG 1 and OG 17, there were no apparent defects in the growth development. Consequently, in comparison with CGMCC 10142, TNA 101ΔagdA and OG 1 decreased the residual reducing sugar by 52.95 and 88.24 %, respectively, and correspondingly increased citric acid production at the end of fermentation by 8.68 and 16.87 %. Citric acid production was further improved by decreasing the non-fermentable residual sugar and increasing utilization rate of corn starch material in A. niger. Besides, the successive saccharification and citric acid fermentation processes were successfully integrated into one step.

ACS Style

Lu Wang; Zhanglei Cao; Li Hou; Liuhua Yin; Dawei Wang; Qiang Gao; Zhenqiang Wu; Depei Wang. The opposite roles of agdA and glaA on citric acid production in Aspergillus niger. Applied Microbiology and Biotechnology 2016, 100, 5791 -5803.

AMA Style

Lu Wang, Zhanglei Cao, Li Hou, Liuhua Yin, Dawei Wang, Qiang Gao, Zhenqiang Wu, Depei Wang. The opposite roles of agdA and glaA on citric acid production in Aspergillus niger. Applied Microbiology and Biotechnology. 2016; 100 (13):5791-5803.

Chicago/Turabian Style

Lu Wang; Zhanglei Cao; Li Hou; Liuhua Yin; Dawei Wang; Qiang Gao; Zhenqiang Wu; Depei Wang. 2016. "The opposite roles of agdA and glaA on citric acid production in Aspergillus niger." Applied Microbiology and Biotechnology 100, no. 13: 5791-5803.

Journal article
Published: 01 January 2015 in Microbial Cell Factories
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The spore germination rate and growth characteristics were compared between the citric acid high-yield strain Aspergillus niger CGMCC 5751 and A. niger ATCC 1015 in media containing antimycin A or DNP. We inferred that differences in citric acid yield might be due to differences in energy metabolism between these strains. To explore the impact of energy metabolism on citric acid production, the changes in intracellular ATP, NADH and NADH/NAD+ were measured at various fermentation stages. In addition, the effects of antimycin A or DNP on energy metabolism and citric acid production was investigated by CGMCC 5751. By comparing the spore germination rate and the extent of growth on PDA plates containing antimycin A or DNP, CGMCC 5751 was shown to be more sensitive to antimycin A than ATCC 1015. The substrate-level phosphorylation of CGMCC 5751 was greater than that of ATCC 1015 on PDA plates with DNP. DNP at tested concentrations had no apparent effect on the growth of CGMCC 5751. There were no apparent effects on the mycelial morphology, the growth of mycelial pellets or the dry cell mass when 0.2 mg L(-1) antimycin A or 0.1 mg L(-1) DNP was added to medium at the 24-h time point. The concentrations of intracellular ATP, NADH and NADH/NAD+ of CGMCC 5751 were notably lower than those of ATCC 1015 at several fermentation stages. Moreover, at 96 h of fermentation, the citric acid production of CGMCC 5751 reached up to 151.67 g L(-1) and 135.78 g L(-1) by adding 0.2 mg L(-1) antimycin A or 0.1 mg L(-1) DNP, respectively, at the 24-h time point of fermentation. Thus, the citric acid production of CGMCC 5751 was increased by 19.89% and 7.32%, respectively. The concentrations of intracellular ATP, NADH and NADH/NAD+ of the citric acid high-yield strain CGMCC 5751 were notably lower than those of ATCC 1015. The excessive ATP has a strong inhibitory effect on citric acid accumulation by A. niger. Increasing NADH oxidation and appropriately reducing the concentration of intracellular ATP can accelerate glycolysis and the TCA cycle to enhance citric acid yield.

ACS Style

Lu Wang; Jianhua Zhang; Zhanglei Cao; Yajun Wang; Qiang Gao; Jian Zhang; Depei Wang. Inhibition of oxidative phosphorylation for enhancing citric acid production by Aspergillus niger. Microbial Cell Factories 2015, 14, 7 .

AMA Style

Lu Wang, Jianhua Zhang, Zhanglei Cao, Yajun Wang, Qiang Gao, Jian Zhang, Depei Wang. Inhibition of oxidative phosphorylation for enhancing citric acid production by Aspergillus niger. Microbial Cell Factories. 2015; 14 (1):7.

Chicago/Turabian Style

Lu Wang; Jianhua Zhang; Zhanglei Cao; Yajun Wang; Qiang Gao; Jian Zhang; Depei Wang. 2015. "Inhibition of oxidative phosphorylation for enhancing citric acid production by Aspergillus niger." Microbial Cell Factories 14, no. 1: 7.