In lab-on-chip devices, on which complete (bio-)chemical analysis laboratories are miniaturized and integrated, it is essential to manipulate fluids in sub-millimetre channels and sub-microlitre chambers. A special challenge in these small micro-fluidic systems is to create good mixing flows, since it is almost impossible to generate turbulence. We propose an active micro-fluidic mixing concept inspired by nature, namely by micro-organisms that swim through a liquid by oscillating microscopic hairs, cilia, that cover their surface. We have fabricated artificial cilia consisting of electro-statically actuated polymer structures, and have integrated these in a micro-fluidic channel. Flow visualization experiments show that the cilia can generate substantial fluid velocities, up to 0.6 mm s⁻¹. In addition, very efficient mixing is obtained using specially designed geometrical cilia configurations in a micro-channel. Since the artificial cilia can be actively controlled using electrical signals, they have exciting applications in micro-fluidic devices.

The demand for easy to use and cost effective medical technologies inspires scientists to develop innovative lab-on-chip technologies for point-of-care in vitro diagnostic testing. To fulfill medical needs, the tests should be rapid, sensitive, quantitative, and miniaturizable, and need to integrate all steps from sample-in to result-out. Here, we review the use of magnetic particles actuated by magnetic fields to perform the different process steps that are required for integrated lab-on-chip diagnostic assays. We discuss the use of magnetic particles to mix fluids, to capture specific analytes, to concentrate analytes, to transfer analytes from one solution to another, to label analytes, to perform stringency and washing steps, and to probe biophysical properties of the analytes, distinguishing methodologies with fluid flow and without fluid flow (stationary microfluidics). Our review focuses on efforts to combine and integrate different magnetically actuated assay steps, with the vision that it will become possible in the future to realize integrated lab-on-chip biosensing assays in which all assay process steps are controlled and optimized by magnetic forces.

In order to study the roles of stress anisotropy and of elasticity in the mechanism of drag reduction by polymer additives we investigate a turbulent pipe flow of a dilute polymer solution. The investigation is carried out by means of direct numerical simulation (DNS) and laser Doppler velocimetry (LDV). In our DNS two different models are used to describe the effects of polymers on the flow. The first is a constitutive equation based on Batchelor's theory of elongated particles suspended in a Newtonian solvent which models the viscous anisotropic effects caused by the polymer orientation. The second is an extension of the first model with an elastic component, and can be interpreted as an anisotropic Maxwell model. The LDV experiments have been carried out in a recirculating pipe flow facility in which we have used a solution of water and 20 w.p.p.m. Superfloc A110. Turbulence statistics up to the fourth moment, as well as power spectra of various velocity components, have been measured. The results of the drag-reduced flow are first compared with those of a standard turbulent pipe flow of water at the same friction velocity at a Reynolds number of Re_τ≈1035. Next the results of the numerical simulation and of the measurements are compared in order to elucidate the role of polymers in the phenomenon of drag reduction. For the case of the viscous anisotropic polymer model, almost all turbulence statistics and power spectra calculated agree in a qualitative sense with the measurements. The addition of elastic effects, on the other hand, has an adverse effect on the drag reduction, i.e. the viscoelastic polymer model shows less drag reduction than the anisotropic model without elasticity. Moreover, for the case of the viscoelastic model not all turbulence statistics show the right behaviour. On the basis of these results, we propose that the viscous anisotropic stresses introduced by extended polymers play a key role in the mechanism of drag reduction by polymer additives.

From February 7–11, 2011, the multidisciplinary Lorentz Workshop Circulating Tumor Cell (CTC) Isolation and Diagnostics: Toward Routine Clinical Use was held in Leiden (The Netherlands) to discuss progress and define challenges and potential solutions for development of clinically useful circulating tumor cell (CTC) diagnostics. CTCs, captured as “liquid biopsy” from blood, for counting and characterization using pathology and molecular assays, are expected to replace metastatic tissue biopsies to be used to predict drug response and resistance and to monitor therapy response and cancer recurrence. CTCs are highly heterogeneous; therefore, cancer type–specific isolation technologies, as well as complex clinical interpretation software, are required. Cancer Res; 71(18); 5955–60. ©2011 AACR.