In an effort to improve and achieve biologically active anticancer agents, a novel series of 1,2,3-triazole-containing hybrids were designed and efficiently synthesized via the Cu-catalyzed azide-alkyne cycloaddition (CuAAC) reaction of substituted-arylazides with alkyne-functionalized pyrazole-[1,2,4]-triazole hybrids. The structure geometry of these new clicked 1,2,3-triazoles was explored by density functional theory (DFT) using the B3LYP/6-311++G(d,p) level; also, the potential activity of the compounds for light absorption was simulated by time-dependent DFT calculations (TD-DFT). The antitumor impacts of the newly synthesized compounds were in vitro estimated to be towards the human liver cancer cell line (HepG-2), the human colon cancer cell line (HCT-116), and human breast adenocarcinoma (MCF-7). Among the tested compounds, conjugate 7 was the most potent cytotoxic candidate towards HepG-2, HCT-116, and MCF-7, with IC₅₀ = 12.22, 14.16, and 14.64 µM, respectively, in comparison to that exhibited by the standard drug doxorubicin (IC₅₀ = 11.21, 12.46, and 13.45 µM). Finally, a molecular docking study was conducted within the epidermal growth factor receptor (EGFR) active site to suggest possible binding modes. Hence, it could conceivably be hypothesized that analogies 7, 6, and 5 could be considered as decent lead candidate compounds for anticancer agents.

The strawberry has a very short postharvest life due to its fast softening and decomposition. The goal of this research is to see how well a starch-silver nanoparticle (St-AgNPs) coating affects the physical, chemical, and microbiological qualities of strawberries during postharvest life. Additionally, the effect of washing with running water on silver concentration in coated strawberry fruit was studied by an inductively coupled plasma-optical emission spectrometer (ICP-OES). Furthermore, the shelf-life period was calculated in relation to the temperature of storage. Fourier transform infrared-attenuated total reflectance (FTIR-ATR), UV-Visible, and Transmission Electron Microscopic (TEM) were used to investigate the structure of starch-silver materials, the size and shape of AgNPs, respectively. The AgNPs were spherical, with an average size range of 12.7 nm. The coated samples had the lowest weight loss, decay, and microbial counts as compared to the uncoated sample. They had higher total acidity and anthocyanin contents as well. The washing process led to the almost complete removal of silver particles by rates ranging from 98.86 to 99.10%. Finally, the coating maintained strawberry qualities and lengthened their shelf-life from 2 to 6 days at room storage and from 8 to 16 days in cold storage.

Arbuscular mycorrhizal fungi (AMF) inoculation and biochar amendment has been reported to improve the growth of several crop plant; however, their role in stress amelioration individually as well as in combination has not been worked out. Limited information is available about the synergistic use of biochar and Arbuscular Mycorrhizal Fungi (AMF). Here, we investigated the synergistic effect of biochar and AMF on plant development, root architecture, the physiological performance of fenugreek (Trigonella foenum-graecum), and soil enzymatic activities. Biochar and AMF were shown to have a considerable effect on plant height, according to the data (53.3 and 66.6%, respectively), leaf number (22.5 and 45.1%), total root length (19.8 and 40.1%), root volume (32.1 and 71.4%), chlorophyll a content (26.0 and 17.8%), chlorophyll b content (50.0 and 28.9%), total chlorophyll content (30.0 and 18.1%), and carotenoid content (60.0 and 48.0%) over the control treatment. There was a considerable increase in plant height when biochar and AMF were combined together by 80.9%, total root length by 68.9%, projected area by 48.7%, root surface area by 34.4%, root volume by 78.5%, chlorophyll a content by 34.2%, chlorophyll b content by 68.4%, total chlorophyll content by 44.5%, and carotenoid content by 84.0% compared to the control. Our results recommend that the combination of biochar and AMF is advantageous in fenugreek growth, microbial biomass, and soil enzyme activities.

Thiazole-substituted pyrazole is an important structural feature of many bioactive compounds, including antiviral, antitubercular, analgesic and anticancer agents. Herein we describe an efficient a... Graphical abstract

This study aimed to investigate the effects of biochar (1%, 2%, and 3%) on seed germination, plant growth, root morphological characteristics, and physiological properties of ginger (Zingiber officinale) and soil enzymatic activities. Pot experiments under greenhouse conditions at 24 °C (day) and 16 °C (night) showed after six weeks that biochar additions of 2% and 3% significantly increased seed germination, plant height, leaf length, leaf number, as well as shoot and root dry weights compared to the control. Total root length significantly increased by 30%, 47%, and 74%, with increasing biochar contents (1%, 2%, and 3%) compared to the control. Root surface area, projected area, root diameter, and root volume reached a maximum at the 3% biochar treatment. The treatment with 2% biochar significantly increased fluorescein diacetate hydrolase and phenoloxidase activities by 33% and 59% compared to the control; so did the addition of 3% biochar, which significantly increased fluorescein diacetate hydrolases, phenoloxidase, and acid and alkaline phosphomonoesterase activity in soil compared to the control. Treatment with 3% biochar increased relative water content by 8%, chlorophyll content by 35%, and carotenoid content by 43% compared to the control. These results suggest that biochar can improve the performance of the rhizome of ginger and increase the activity of soil enzymes, thereby improving soil nutrient supply.

6-Hydroxy-2-oxo-2H-chromene-4-carbaldehyde (2), 6-chloro-2-oxo-2H-chromene-4-carbaldehyde (3) and 6-hydrazinyl-4-methyl-2H-chromen-2-one (5) were prepared as single-pharmacophore motif key intermediates. Compounds 2, 3 and 5 were incorporated in a series of multicomponent reactions (MCRs), under microwave assistance as well as conventional chemical synthesis processes, to afford a series of three and/or four-pharmacophoric-motif conjugates 8a,b, 11, 13, 16, 17, 19 and 20 in good yields. The newly synthesized compounds were characterized by IR, NMR, ¹³C-NMR, MS and elemental analyses. Finally the synthesized compounds have been screened for their biological activity whereupon they exhibited remarkable antimicrobial activity on different classes of bacteria and the fungus.