In temperate regions of Europe, the large pine weevil, Hylobius abietis, is a major pest of coniferous forests mostly at sites where clear-felling is followed by planting of saplings. Control measures against this pest are based on silvicultural techniques, an application of physical barriers on stems of saplings and insecticide treatments. To avoid the use of insecticides, alternative measures such as biological control have been investigated. The goal of the present study was to obtain local strains of entomopathogenic fungi (Ascomycota, Hypocreales) from natural populations of H. abietis, and to investigate their efficacy against the weevil. A survey on entomopathogenic fungi was undertaken at clear-felled areas of spruce forests in northern Slovakia. Two Beauveria species, B. bassiana and B. pseudobassiana, were identified, and 22 in vitro strains were obtained. Mean prevalence of infected adults was low (2.10% ± 0.67%) and the mycosis was mostly recorded during May and June. Virulence of Beauveria strains against the weevil was tested in laboratory. B. bassiana strain AMEP20 was significantly most virulent (LC₅₀ of 0.65 ± 0.10 × 10⁸ conidia mL⁻¹). Treatment with conidia of AMEP20 strain affected feeding damage by the weevil on bark of Scots pine twigs. Daily bark consumption by B. bassiana-treated weevils was lower than by untreated individuals and decreased with increasing conidia concentration used for the treatment. In the outdoor experiment, AMEP20 strain killed weevils that fed on spruce saplings treated with conidia suspensions. Mortality due to mycosis on weevils exposed to the conidia-treated saplings reached 30.0%–76.5% and 55.0%–88.2% after 32 and 46 days, respectively.

The large pine weevil Hylobius abietis (Linnaeus 1758) is the main pest of coniferous seedlings in Europe and causes substantial damage in areas that have been clear-cut or otherwise disturbed. We compared the efficacy of different attractants for the capture of H. abietis adults in white pitfall traps. The field experiment was performed from mid-April to the end of August 2018 at six plots in Central Europe located in spruce stands that had been clear-cut. At each plot, we compared five attractants: one pine twig with ethanol, Hylodor, alpha-pinene + ethanol, turpentine oil and ethanol (separated), and turpentine oil + ethanol (not separated). Traps without attractant served as a control. Six traps for each attractant or control were distributed at each plot. Of the total number of H. abietis adults trapped, 43.3%, 20.5%, 17.9%, 9.8%, 8.5% and 0.5% were captured in traps with pine twigs with ethanol, alpha-pinene, Hylodor, turpentine, oil + ethanol (separated), turpentine oil + ethanol (combined), and no attractant, respectively. The bottom of each trap contained propylene glycol to kill and preserve beetles. The small number of beetles captured in the control traps confirms that the propylene glycol:water mixture did not influence the trapping of H. abietis. The use of pitfall traps with a suitable attractant (especially pine twigs and ethanol) should be useful for monitoring of H. abietis, because it is simple and cost-effective. The use of such pitfall traps to control H. abietis by mass trapping would require 50 to 100 traps per ha.

The emerald ash borer, Agrilus planipennis Fairmaire, 1888 (Coleoptera: Buprestidae), is an invasive pest in Europe and North America, responsible for extensive mortality of ash (Fraxinus spp.). This study reports its first confirmed occurrence in Slovakia and summarises the subsequent delimiting survey. On 25 June 2026, specimens morphologically resembling A. planipennis were collected from a monitoring trap in a park in Streda nad Bodrogom, south-eastern Slovakia. Eighteen adults were identified as A. planipennis by the national quarantine diagnostics laboratory, with confirmation by the European Union Reference Laboratory in France. Initial field inspections revealed characteristic galleries and D-shaped exit holes on ash trees at the detection site, in nearby forests, and along roads within approximately 7 km. By 31 July 2026, 2250 survey points had been recorded, of which 149 showed evidence of A. planipennis, including adults, larvae, galleries, or exit holes. Old galleries together with living larvae indicate that the pest had probably been established in Slovakia for several years before detection in 2026. Symptom assessment was complicated by widespread ash dieback caused by Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz & Hosoya, 2014.

Gnathotrichus materiarius Fitch, 1858 is a North American ambrosia beetle that has progressively expanded its distribution across Europe during the twentieth and twenty-first centuries. The aim of this study was to assess its occurrence in Slovakia based on multi-year surveys conducted in the Záhorie region and to document its first national record. Monitoring was carried out in 2021 and 2025 in pine-dominated stands using traps baited with pheromone and kairomone attractants. No individuals were detected in 2021. In 2025, two adults of G. materiarius were captured at a single site during the first inspection of traps baited with a (+)-α-pinene and ethanol blend. A large volume of stored pine timber was present in close proximity to the capture site, providing suitable breeding material. The findings suggest a recent introduction or early stage of establishment in Slovakia. Although the species is regarded as a technical pest of coniferous wood, it does not colonize healthy trees and currently poses a limited risk to vital forest stands. Continued targeted monitoring is important to assess its further spread.