The attenuation of soil organic carbon and the destruction of soil microbial structure are common manifestations of grassland degradation. The addition of exogenous organic carbon and microorganisms may be an effective way to quickly restore degraded grassland, but corresponding evaluations are still rare. We investigated the effects of effective microorganisms (EM) and biochar addition on vegetation biomass, microorganisms and soil properties in degraded alpine grassland. The treatments included a control (no biochar or EM addition, CK), EM addition (250 mL m⁻² EM, M), biochar addition (4.00 kg m⁻² biochar, C) and a mixture of biochar and EM (4.00 kg m⁻² biochar and 250 mL m⁻² EM, C+M). C, M and C+M rapidly increased vegetation biomass, soil organic carbon (TOC), total nitrogen (TN), available nitrogen (NH₄⁺-N, NO₃⁻-N), available phosphorus (AP), total microbial biomass (MB), bacteria and fungus biomass in the soil, and also altered the microbial community structure. The content of soil nutrients in the C treatment was the highest, followed by C+M. The vegetation biomass and microbial biomass were the greatest in the C+M treatment, and increased by 101.04~198.52% and 22.14~45.41%, respectively. C+M can also enhance the presence of saprotrophic fungi, thereby facilitating the augmentation of both plant and soil nutrients. Overall, the biochar combined with EM addition had a synergistic effect on the restoration of degraded alpine grasslands.

Being subject to climate change and human intervention, the land-use pattern in the agro-pastoral ecotone of Northern China has undergone complex changes over the past few decades, which may jeopardize the provision of ecosystem services. Thus, for sustainable land management, ecosystem services should be evaluated and monitored. In this study, based on Landsat TM/ETM data, we quantitatively evaluated the losses of ecosystem service values (ESV) in three sections of the agro-pastoral ecotone from 1980–2015. The results were as follows: (1) the main characteristic of the land conversions was that a large area of grassland was converted into cultivated land in the agro-pastoral ecotone; (2) on the spatial scale, the ESV losses of the agro-pastoral ecotone can be called an “inclined surface” in the direction of the northeast to southwest, and the northeastern section of the agro-pastoral ecotone lost more ESV than the middle and northwest sections (p < 0.05), on the temporal scale, the order of losses was 1990–2000 > 1980–1990 > 2000–2015; (3) the agro-pastoral ecotone lost more ESV, which was mainly due to four kinds of land conversion, which were grassland that was transformed into cultivated land, grassland transformed into unused land, grassland transformed into built-up areas, and cultivated land transformed into built-up areas; (4) although these land conversions were curbed after the implementation of protection policies at the end of the 1990s, due to reduced precipitation and increasing temperatures, the agro-pastoral ecotone will face a more severe situation in the future; and, (5) during the period of 1990–2015, the overall dynamic processes of increasing population gradually expanded to the sparsely populated pastoral area. Therefore, we believe that human interventions are the main cause of ecological deterioration in the agro-pastoral ecotone. This study provides references for fully understanding the regional differences in the ecological and environmental effects of land use change and it helps to objectively evaluate ecological civilization construction in the agro-pastoral ecotone of Northern China.

Soil beneficial microorganism deficiency in the degraded grasslands have emerged as the major factors negatively impacting soil quality and vegetation productivity. EM (effective microorganisms) has been regarded as a good ameliorant in improving microbial communities and restoring degraded soil of agricultural systems. However, knowledge was inadequate regarding the effects of adding EM on the degraded alpine grassland. Four levels of EM addition (0, 150, 200, 250 mL m^–2) were conducted to investigate the effects of EM addition on soil properties and microorganisms of degraded alpine grassland. The addition of EM increased aboveground biomass, soil organic carbon, total nitrogen, available phosphorus, and microbial biomass, but decreased soil electric conductivity. Meanwhile, the relative biomasses of gram-negative bacteria decreased, while the ectomycorrhizal fungi and arbuscular mycorrhizal fungi increased after EM addition. The relationship between microbial communities and environmental factors has been changed. The restore effect of EM increased with the increase of addition time. These results indicated that EM addition could be a good practice to restore the health of the degraded alpine grassland ecosystem.

The distribution of soil particle sizes is closely related to soil health condition. In this study, grasslands under different grazing intensities and different cultivation ages grasslands were selected to evaluate the dynamics of soil particle size redistribution in different soil layers. When the grazing intensity increased, the percentage of 2000~150-μm soil particles in the 0–10-cm soil layer decreased; 150~53-μm soil particles remained relatively stable among the grazing intensities—approximately 28.52%~35.39%. However, the percentage of less than 53-μm soil particles increased. In cultivated grasslands, the larger sizes (>53 μm) of soil particles increased and the smaller sizes (<53 μm) decreased significantly (p < 0.05) in the 0–10 cm-soil layer with increasing cultivation ages. The increase in small soil particles (53 μm) in topsoil associated with cultivation ages decreased the soil capacity of holding water and nutrient. Therefore, to maintain the sustainability of grassland uses, grazing grasslands need to avoid heavy grazing, and cultivated grasslands need to change current cultivation practices.