The exposure of concrete to high temperatures, such as in a fire, leads to physical and chemical changes, which may cause deterioration of mechanical properties, cracking and spalling. This paper presents a critical review of microstructural changes in concrete exposed to high temperature. The transformations developed in the cement paste, aggregates and interfacial transition zone were studied, as well as the experimental techniques of microanalysis presented in recent related researches. Lastly, a critical analysis of experimental results from literature was performed. It was verified that microstructural changes are related to concrete properties and the heating process. The experimental techniques has a potential use for assessment of thermally damaged concrete, however, these techniques must be applied simultaneously and specific methods must be established.