A 2-year pot experiment was carried out to examine the aging effect of biochar (B), alone or combined with iron grit (Z), on Cu stabilization and plant growth in a contaminated soil (964 mg Cu kg(-1)) from a wood preservation site. The experiment consisted in 3 soil treatments, either planted with Arundo donax L. (Ad) or Populus nigra L (Pn): (1) untreated Cu-contaminated soil (Ad, Pn); (2) Unt + 1% (w/w) B (AdB, PnB), and (3) Unt + 1% B + 1% Z (AdBZ, PnBZ). After 22 months, the soil pore water (SPW) was sampled and roots and shoots were harvested. The SPW compositions at 3 and 22 months were compared, showing that the SPW Cu2+ concentration increased again in the PnB and PnBZ soils. Cultivation of A. donax enhanced the dissolved organic matter concentration in the SPW, which decreased its Cu2+ concentration but promoted its total Cu concentration in theAd and AdB soils. Adding Z with B reduced both SPW Cu2+ and Cu concentrations in the pots cultivated by A. donax and P. nigra as compared to B alone. The B and BZ treatments did not enhance root and shoot yields of both plant species as compared to the Unt soil but their shoot Cu concentrations were in the range of common values. (C) 2016 Elsevier B.V. All rights reserved.