A study was conducted in an irrigated arid agroecosystem in southwestern USA, to compare two conservation tillage systems (strip tillage (ST) and no-tillage (NT)) to conventional, plow-based tillage (PT) system. Corn silage (Zea mays L.) was planted in this trial. Growth parameters (plant population and height) of corn silage were measured during the season and yield was evaluated at harvest. Soil physical measurements assessed included mean weight diameter of dry aggregates, wet aggregate stability, and penetrometer resistance. While soil biological measurements included total microbial biomass, diversity index (DI), total bacteria biomass, total fungi biomass (TFB), arbuscular mycorrhizae fungi (AMF), and total saprophytes. Results showed that plant population and silage yield at 65% moisture content were not significant with tillage during both trial years. Soil physical parameters were mostly not significant with tillage, while three out of the six biological measurements (DI, TF, and AM) were significant with tillage at p ≤ 0.05. No-tillage had higher DI and TFB than the ST, but not different from PT, while AMF was significantly higher in PT than ST, but not different from NT. The study demonstrates that farmers in the study region can adopt conservation tillage without yield losses during the early years of transition.