Alfonso Blázquez-Castro

Dr. Alfonso Blázquez-Castro

Department of Biology, Faculty of Sciences, Autonomous University of Madrid, 28049 Madrid, Spain
orcid

Research Focus & Metrics

Academic Career Length

19Years (2008-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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1 April 2018 in Acta Histochemica
Verified
Journal Article
Closed access
JSJuan Carlos Stockert
Juan Carlos Stockert
RHRichard W Horobin
Richard W Horobin
LCLucas L. Colombo
Lucas L. Colombo
Alfonso Blázquez-CastroAlfonso Blázquez-Castro
Alfonso Blázquez-Castro

1 December 2012 in Acta Histochemica
Verified
Journal Article
Closed access
JSJuan C. Stockert
Juan C. Stockert
Alfonso Blázquez-CastroAlfonso Blázquez-Castro
Alfonso Blázquez-Castro
MCMagdalena Cañete
Magdalena Cañete
RHRichard W. Horobin
Richard W. Horobin
ÁVÁngeles Villanueva
Ángeles Villanueva

17 November 2009 in Nanoscale
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Journal Article
Closed access
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FVFiorenzo Vetrone
Fiorenzo Vetrone
RNRafik Naccache
Rafik Naccache
Angeles JuarranzAngeles Juarranz
Angeles Juarranz
FSFrancisco Sanz-Rodríguez
Francisco Sanz-Rodríguez
Alfonso Blázquez-CastroAlfonso Blázquez-Castro
Alfonso Blázquez-Castro
JCJohn Capobianco
John Capobianco
We report on the efficient incorporation of non-functionalized NaYF4 : Er3+, Yb3+ nanoparticles inside HeLa live cancer cells by direct endocytosis. The efficient two-photon excited near-infrared-to-visible upconversion fluorescence of these nanoparticles is then used to obtain high-contrast intracellular fluorescence images of single cells. These images reveal a redistribution of the nanoparticles inside the cell as the incubation time increases. Thus, non-functionalized NaYF4 : Er3+, Yb3+ nanoparticles emerge as very promising fluorescence probes for real-time imaging of cellular dynamics.

31 July 2019 in Micromachines
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Journal Article
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Alfonso Blázquez-CastroAlfonso Blázquez-Castro
Alfonso Blázquez-Castro
For several decades optical tweezers have proven to be an invaluable tool in the study and analysis of myriad biological responses and applications. However, as with every tool, they can have undesirable or damaging effects upon the very sample they are helping to study. In this review the main negative effects of optical tweezers upon biostructures and living systems will be presented. There are three main areas on which the review will focus: linear optical excitation within the tweezers, non-linear photonic effects, and thermal load upon the sampled volume. Additional information is provided on negative mechanical effects of optical traps on biological structures. Strategies to avoid or, at least, minimize these negative effects will be introduced. Finally, all these effects, undesirable for the most, can have positive applications under the right conditions. Some hints in this direction will also be discussed.

28 September 2018 in Applied Physics Reviews
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Journal Article
Open access
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Alfonso Blázquez-CastroAlfonso Blázquez-Castro
Alfonso Blázquez-Castro
AGA. García-Cabañes
A. García-Cabañes
MCMercedes Carrascosa
Mercedes Carrascosa
The study and applications of ferroelectric materials in the biomedical and biotechnological fields is a novel and very promising scientific area that spans roughly one decade. However, some groups have already provided experimental proof of very interesting biological modulation when living systems are exposed to different ferroelectrics and excitation mechanisms. These materials should offer several advantages in the field of bioelectricity, such as no need of an external electric power source or circuits, scalable size of the electroactive regions, flexible and reconfigurable “virtual electrodes,” or fully proved biocompatibility. In this focused review, we provide the underlying physics of ferroelectric activity and a recount of the research reports already published, along with some tentative biophysical mechanisms that can explain the observed results. More specifically, we focus on the biological actions of domain ferroelectrics and ferroelectrics excited by the bulk photovoltaic effect or the pyroelectric effect. It is our goal to provide a comprehensive account of the published material so far and to set the stage for a vigorous expansion of the field, with envisioned applications that span from cell biology and signaling to cell and tissue regeneration, antitumoral action, or cell bioengineering to name a few.

1 October 2017 in Redox Biology
Verified
Journal Article
Open access
Alfonso Blázquez-CastroAlfonso Blázquez-Castro
Alfonso Blázquez-Castro
Molecular oxygen (O2) displays very interesting properties. Its first excited state, commonly known as singlet oxygen (1O2), is one of the so-called Reactive Oxygen Species (ROS). It has been implicated in many redox processes in biological systems. For many decades its role has been that of a deleterious chemical species, although very positive clinical applications in the Photodynamic Therapy of cancer (PDT) have been reported. More recently, many ROS, and also 1O2, are in the spotlight because of their role in physiological signaling, like cell proliferation or tissue regeneration. However, there are methodological shortcomings to properly assess the role of 1O2 in redox biology with classical generation procedures. In this review the direct optical excitation of O2 to produce 1O2 will be introduced, in order to present its main advantages and drawbacks for biological studies. This photonic approach can provide with many interesting possibilities to understand and put to use ROS in redox signaling and in the biomedical field.