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Wheat is a major staple food crop worldwide because of the unique properties of wheat flour. High molecular weight glutenin subunits (HMW-GSs), which are among the most critical determinants of wheat flour quality, are responsible for the formation of glutenin polymeric structures via interchain disulfide bonds. We herein describe the identification of a new HMW-GS Dy10 allele (Dy10-m619SN). The amino acid substitution (serine-to-asparagine) encoded in this allele resulted in a partial post-translational cleavage that produced two new peptides. These new peptides disrupted the interactions among gluten proteins because of the associated changes to the number of available cysteine residues for interchain disulfide bonds. Consequently, Dy10-m619SN expression decreased the size of glutenin polymers and weakened glutens, which resulted in wheat dough with improved cookie-making quality, without changes to the glutenin-to-gliadin ratio. In this study, we clarified the post-translational processing of HMW-GSs and revealed a new genetic resource useful for wheat breeding.
Yan Wang; Qing Chen; Yang Li; Zhenru Guo; Caihong Liu; Yongfang Wan; Malcolm Hawkesford; Jing Zhu; Wang Wu; Meiqiao Wei; Kan Zhao; Yunfeng Jiang; Yazhou Zhang; Qiang Xu; Li Kong; Zhien Pu; Mei Deng; Qiantao Jiang; Xiujin Lan; Jirui Wang; Guoyue Chen; Jian Ma; Youliang Zheng; Yuming Wei; Pengfei Qi. Post-translational cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum). Molecular Breeding 2021, 41, 1 -15.
AMA StyleYan Wang, Qing Chen, Yang Li, Zhenru Guo, Caihong Liu, Yongfang Wan, Malcolm Hawkesford, Jing Zhu, Wang Wu, Meiqiao Wei, Kan Zhao, Yunfeng Jiang, Yazhou Zhang, Qiang Xu, Li Kong, Zhien Pu, Mei Deng, Qiantao Jiang, Xiujin Lan, Jirui Wang, Guoyue Chen, Jian Ma, Youliang Zheng, Yuming Wei, Pengfei Qi. Post-translational cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum). Molecular Breeding. 2021; 41 (8):1-15.
Chicago/Turabian StyleYan Wang; Qing Chen; Yang Li; Zhenru Guo; Caihong Liu; Yongfang Wan; Malcolm Hawkesford; Jing Zhu; Wang Wu; Meiqiao Wei; Kan Zhao; Yunfeng Jiang; Yazhou Zhang; Qiang Xu; Li Kong; Zhien Pu; Mei Deng; Qiantao Jiang; Xiujin Lan; Jirui Wang; Guoyue Chen; Jian Ma; Youliang Zheng; Yuming Wei; Pengfei Qi. 2021. "Post-translational cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum)." Molecular Breeding 41, no. 8: 1-15.
Wheat is a major staple food crop worldwide, due to its total yield and unique processing quality. Its grain yield and quality are threatened by Fusarium head blight (FHB), which is mainly caused by Fusarium graminearum. Salicylic acid (SA) has a strong and toxic effect on F. graminearum and is a hopeful target for sustainable control of FHB. F. graminearum is capable of efficientdealing with SA stress. However, the underlying mechanisms remain unclear. Here, we characterized FgMFS1 (FGSG_03725), a major facilitator superfamily (MFS) transporter gene in F. graminearum. FgMFS1 was highly expressed during infection and was upregulated by SA. The predicted three-dimensional structure of the FgMFS1 protein was consistent with the schematic for the antiporter. The subcellular localization experiment indicated that FgMFS1 was usually expressed in the vacuole of hyphae, but was alternatively distributed in the cell membrane under SA treatment, indicating an element of F. graminearum in response to SA. ΔFgMFS1 (loss of function mutant of FgMFS1) showed enhanced sensitivity to SA, less pathogenicity towards wheat, and reduced DON production under SA stress. Re-introduction of a functional FgMFS1 gene into ∆FgMFS1 recovered the mutant phenotypes. Wheat spikes inoculated with ΔFgMFS1 accumulated more SA when compared to those inoculated with the wild-type strain. Ecotopic expression of FgMFS1 in yeast enhanced its tolerance to SA as expected, further demonstrating that FgMFS1 functions as an SA exporter. In conclusion, FgMFS1 encodes an SA exporter in F. graminearum, which is critical for its response to wheat endogenous SA and pathogenicity towards wheat.
Qing Chen; Lu Lei; Caihong Liu; Yazhou Zhang; Qiang Xu; Jing Zhu; Zhenru Guo; Yan Wang; Qingcheng Li; Yang Li; Li Kong; Yunfeng Jiang; Xiujin Lan; Jirui Wang; Qiantao Jiang; Guoyue Chen; Jian Ma; Yuming Wei; Youliang Zheng; Pengfei Qi. Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat. International Journal of Molecular Sciences 2021, 22, 8497 .
AMA StyleQing Chen, Lu Lei, Caihong Liu, Yazhou Zhang, Qiang Xu, Jing Zhu, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Li Kong, Yunfeng Jiang, Xiujin Lan, Jirui Wang, Qiantao Jiang, Guoyue Chen, Jian Ma, Yuming Wei, Youliang Zheng, Pengfei Qi. Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat. International Journal of Molecular Sciences. 2021; 22 (16):8497.
Chicago/Turabian StyleQing Chen; Lu Lei; Caihong Liu; Yazhou Zhang; Qiang Xu; Jing Zhu; Zhenru Guo; Yan Wang; Qingcheng Li; Yang Li; Li Kong; Yunfeng Jiang; Xiujin Lan; Jirui Wang; Qiantao Jiang; Guoyue Chen; Jian Ma; Yuming Wei; Youliang Zheng; Pengfei Qi. 2021. "Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat." International Journal of Molecular Sciences 22, no. 16: 8497.
The basic leucine zipper (bZIP) family of genes encode transcription factors that play key roles in plant growth and development. In this study, a total of 92 HvbZIP genes were identified and compared with previous studies using recently released barley genome data. Two novel genes were characterized in this study, and some misannotated and duplicated genes from previous studies have been corrected. Phylogenetic analysis results showed that 92 HvbZIP genes were classified into 10 groups and three unknown groups. The gene structure and motif distribution of the three unknown groups implied that the genes of the three groups may be functionally different. Expression profiling indicated that the HvbZIP genes exhibited different patterns of spatial and temporal expression. Using qRT-PCR, more than 10 HvbZIP genes were identified with expression patterns similar to those of starch synthase genes in barley. Yeast one-hybrid analysis revealed that two of the HvbZIP genes exhibited in vitro binding activity to the promoter of HvAGP-S. The two HvbZIP genes may be candidate genes for further study to explore the mechanism by which they regulate the synthesis of barley starch.
Xiaojuan Zhong; Xiuqin Feng; Yulong Li; Carlos Guzmán; Na Lin; Qiang Xu; Yazhou Zhang; Huaping Tang; Pengfei Qi; Mei Deng; Jian Ma; Jirui Wang; Guoyue Chen; Xiujin Lan; Yuming Wei; Youliang Zheng; Qiantao Jiang. Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley (Hordeum vulgare L.). Genome 2021, 1 -14.
AMA StyleXiaojuan Zhong, Xiuqin Feng, Yulong Li, Carlos Guzmán, Na Lin, Qiang Xu, Yazhou Zhang, Huaping Tang, Pengfei Qi, Mei Deng, Jian Ma, Jirui Wang, Guoyue Chen, Xiujin Lan, Yuming Wei, Youliang Zheng, Qiantao Jiang. Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley (Hordeum vulgare L.). Genome. 2021; ():1-14.
Chicago/Turabian StyleXiaojuan Zhong; Xiuqin Feng; Yulong Li; Carlos Guzmán; Na Lin; Qiang Xu; Yazhou Zhang; Huaping Tang; Pengfei Qi; Mei Deng; Jian Ma; Jirui Wang; Guoyue Chen; Xiujin Lan; Yuming Wei; Youliang Zheng; Qiantao Jiang. 2021. "Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley (Hordeum vulgare L.)." Genome , no. : 1-14.
Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici, is one of the most destructive diseases of wheat worldwide. Chinese wheat landrace Guangtoumai (GTM) exhibited a high level of resistance against predominant P. striiformis f. sp. tritici races in China at the adult plant stage. The objective of this research was to identify and map the major locus/loci for stripe rust resistance in GTM. A set of 212 recombinant inbred lines (RILs) was developed from a cross between GTM and Avocet S. The parents and RILs were evaluated in three field tests (2018, 2019, and 2020 at Chongzhou, Sichuan) with the currently predominant P. striiformis f. sp. tritici races for final disease severity and genotyped with the Wheat 55K single nucleotide polymorphism (SNP) array to construct a genetic map with 1,031 SNP markers. A major locus, named QYr.GTM-5DL, was detected on chromosome 5DL in GTM. The locus was mapped in a 2.75-cM interval flanked by SNP markers AX-109855976 and AX-109453419, explaining up to 44.4% of the total phenotypic variation. Since no known Yr genes have been reported on chromosome 5DL, QYr.GTM-5DL is very likely a novel adult plant resistance locus. Haplotype analysis revealed that the resistance allele displayed enhanced levels of stripe rust resistance and is likely present in 5.3% of the 247 surveyed Chinese wheat landraces. The derived cleaved amplified polymorphic sequence (dCAPS) marker dCAPS-5722, converted from a SNP marker tightly linked to QYr.GTM-5DL with 0.3 cM, was validated on a subset of RILs and 48 commercial wheat cultivars developed in Sichuan. The results indicated that QYr.GTM-5DL with its linked dCAPS marker could be used in marker-assisted selection to improve stripe rust resistance in breeding programs, and this quantitative trait locus will provide new and possibly durable resistance to stripe rust.
Yu Wu; Yuqi Wang; Fangjie Yao; Li Long; Jing Li; Hao Li; Zhien Pu; Wei Li; Qian-Tao Jiang; Jirui Wang; Yu-Ming Wei; Jian Ma; Houyang Kang; Pengfei Qi; Shoufen Dai; Mei Deng; You-Liang Zheng; Yunfeng Jiang; Guo-Yue Chen. Molecular Mapping of a Novel Quantitative Trait Locus Conferring Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Guangtoumai. Plant Disease 2021, 1 -07.
AMA StyleYu Wu, Yuqi Wang, Fangjie Yao, Li Long, Jing Li, Hao Li, Zhien Pu, Wei Li, Qian-Tao Jiang, Jirui Wang, Yu-Ming Wei, Jian Ma, Houyang Kang, Pengfei Qi, Shoufen Dai, Mei Deng, You-Liang Zheng, Yunfeng Jiang, Guo-Yue Chen. Molecular Mapping of a Novel Quantitative Trait Locus Conferring Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Guangtoumai. Plant Disease. 2021; ():1-07.
Chicago/Turabian StyleYu Wu; Yuqi Wang; Fangjie Yao; Li Long; Jing Li; Hao Li; Zhien Pu; Wei Li; Qian-Tao Jiang; Jirui Wang; Yu-Ming Wei; Jian Ma; Houyang Kang; Pengfei Qi; Shoufen Dai; Mei Deng; You-Liang Zheng; Yunfeng Jiang; Guo-Yue Chen. 2021. "Molecular Mapping of a Novel Quantitative Trait Locus Conferring Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Guangtoumai." Plant Disease , no. : 1-07.
Tiller angle (TA) strongly influences plant architecture and grain yield in cereals. However, the genetic basis of TA in wheat is largely unknown. We identified three TA-related quantitative trait loci (QTL). One of them was QTa.sau-2B-769, a major QTL localized on chromosome arm 2BL. QTa.sau-2B-769 was detected in seven environments, explaining 18.1%–51.1% of phenotypic variance. We developed a linked Kompetitive Allele-Specific Polymerase chain reaction (KASP) marker, KASP-AX-108792274, to further validate this locus in three additional populations in multiple environments. QTa.sau-2B-769 increased TA by up to 24.9% in these populations. There were significant and positive correlations between TA and flag leaf angle (FLANG). However, TA was not correlated with plant height or anthesis date, suggesting that expression of QTa.sau-2B-769 is independent of vernalization. TraesCS2B01G583800, a gene known to be involved in leaf angle regulation, was identified as the most likely candidate gene for QTa.sau-2B-769. These results enrich our understanding of the mechanisms regulating wheat TA at maturity and may support precise mapping and cloning of gene(s) underlying QTa.sau-2B-769.
Jiajun Liu; Jieguang Zhou; Huaping Tang; Yang Tu; Yang Mu; Lulu Gou; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Pengfei Qi; Wei Li; Yunfeng Jiang; Zehong Yan; Houyang Kang; Yuming Wei; Xiujin Lan; Youliang Zheng; Jian Ma. A major vernalization-independent QTL for tiller angle on chromosome arm 2BL in bread wheat. The Crop Journal 2021, 1 .
AMA StyleJiajun Liu, Jieguang Zhou, Huaping Tang, Yang Tu, Yang Mu, Lulu Gou, Qiantao Jiang, Yaxi Liu, Guoyue Chen, Jirui Wang, Pengfei Qi, Wei Li, Yunfeng Jiang, Zehong Yan, Houyang Kang, Yuming Wei, Xiujin Lan, Youliang Zheng, Jian Ma. A major vernalization-independent QTL for tiller angle on chromosome arm 2BL in bread wheat. The Crop Journal. 2021; ():1.
Chicago/Turabian StyleJiajun Liu; Jieguang Zhou; Huaping Tang; Yang Tu; Yang Mu; Lulu Gou; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Pengfei Qi; Wei Li; Yunfeng Jiang; Zehong Yan; Houyang Kang; Yuming Wei; Xiujin Lan; Youliang Zheng; Jian Ma. 2021. "A major vernalization-independent QTL for tiller angle on chromosome arm 2BL in bread wheat." The Crop Journal , no. : 1.
Anthocyanins and selenium have vital biological functions for human and plants, they were investigated thoroughly and separately in plants. Previous studies indicated pigmented fruits and vegetables had higher selenium concentration, but whether there is a relationship between anthocyanins and selenium is unclear. In this study, a combined phenotypic and genotypic methodological approach was undertaken to explore the potential relationship between anthocyanins and selenium accumulation by using phenotypic investigation and RNA-seq analysis. The results showed that pigmented cultivars enrichment in Se is a general phenomenon observed for these tested species, this due to pigmented cultivars have higher Se efficiency absorption. And Se flow direction mainly improve concentration of S-rich proteins of LMW-GS. This may be a result of the MYB and bHLH co-regulate anthocyanins biosynthesis and Se metabolism at the transcriptional level. This thesis addresses a neglected aspect of the relevant relationship between anthocyanins and selenium.
Zhien Pu; Guanghui Wei; Zehou Liu; Li Chen; Han Guo; Yang Li; Yong Li; Shoufen Dai; Jirui Wang; Wei Li; Qiantao Jiang; Yuming Wei; Youliang Zheng. Selenium and anthocyanins share the same transcription factors R2R3MYB and bHLH in wheat. Food Chemistry 2021, 356, 129699 .
AMA StyleZhien Pu, Guanghui Wei, Zehou Liu, Li Chen, Han Guo, Yang Li, Yong Li, Shoufen Dai, Jirui Wang, Wei Li, Qiantao Jiang, Yuming Wei, Youliang Zheng. Selenium and anthocyanins share the same transcription factors R2R3MYB and bHLH in wheat. Food Chemistry. 2021; 356 ():129699.
Chicago/Turabian StyleZhien Pu; Guanghui Wei; Zehou Liu; Li Chen; Han Guo; Yang Li; Yong Li; Shoufen Dai; Jirui Wang; Wei Li; Qiantao Jiang; Yuming Wei; Youliang Zheng. 2021. "Selenium and anthocyanins share the same transcription factors R2R3MYB and bHLH in wheat." Food Chemistry 356, no. : 129699.
Subgenome asymmetry (SA) has routinely been attributed to different responses between the subgenomes of a polyploid to various stimuli during evolution. Here, we compared subgenome differences in gene ratio and relative diversity between artificial and natural genotypes of several allopolyploid species. Surprisingly, consistent differences were not detected between these two types of polyploid genotypes, although they differ in times exposed to evolutionary selection. The estimated ratio of shared genes between a subgenome and its diploid donor was invariably higher for the artificial allopolyploid genotypes than those for the natural genotypes, which is expected as it is now well-known that many genes in a species are not shared among all individuals. As the exact diploid parent for a given subgenome is unknown, the estimated ratios of shared genes for the natural genotypes would also include difference among individual genotypes of the diploid donor species. Further, we detected the presence of SA in genotypes before the completion of the polyploidization events as well as in those which were not formed via polyploidization. These results indicate that SA may, to a large degree, reflect differences between its diploid donors or that changes occurred during polyploid evolution are defined by their donor genomes.
Xueling Ye; Haiyan Hu; Hong Zhou; Yunfeng Jiang; Shang Gao; Zhongwei Yuan; Jiri Stiller; Chengwei Li; Guoyue Chen; Yaxi Liu; Yuming Wei; You-Liang Zheng; You-Gan Wang; Chunji Liu. Differences between diploid donors are the main contributing factor for subgenome asymmetry measured in either gene ratio or relative diversity in allopolyploids. Genome 2021, 1 -10.
AMA StyleXueling Ye, Haiyan Hu, Hong Zhou, Yunfeng Jiang, Shang Gao, Zhongwei Yuan, Jiri Stiller, Chengwei Li, Guoyue Chen, Yaxi Liu, Yuming Wei, You-Liang Zheng, You-Gan Wang, Chunji Liu. Differences between diploid donors are the main contributing factor for subgenome asymmetry measured in either gene ratio or relative diversity in allopolyploids. Genome. 2021; ():1-10.
Chicago/Turabian StyleXueling Ye; Haiyan Hu; Hong Zhou; Yunfeng Jiang; Shang Gao; Zhongwei Yuan; Jiri Stiller; Chengwei Li; Guoyue Chen; Yaxi Liu; Yuming Wei; You-Liang Zheng; You-Gan Wang; Chunji Liu. 2021. "Differences between diploid donors are the main contributing factor for subgenome asymmetry measured in either gene ratio or relative diversity in allopolyploids." Genome , no. : 1-10.
Chinese wheat landrace Anyuehong (AYH) has displayed high levels of stable adult-plant resistance (APR) to stripe rust for more than 15 years. To identify QTL for stripe rust resistance in AYH, a set of 110 recombinant inbred lines (RILs) was developed from a cross between AYH and susceptible cultivar Taichung 29. The parents and RILs were evaluated for final disease severity (FDS) in six field tests with a mixture of predominant Pst races at the adult-plant stage and genotyped using the wheat 55K SNP array to construct a genetic map with 1,143 SNP markers. Three QTL, designated as QYr.AYH-1AS, QYr.AYH-5BL and QYr.AYH-7DS, were mapped on chromosome 1AS, 5BL and 7DS, respectively. RILs combining three QTL showed significantly reduced FDS compared with the lines in other combinations. Of them, QYr.AYH-5BL and QYr.AYH-7DS were stably detected in all environments, explaining 13.6–21.4% and 17.6–33.6% of phenotypic variation, respectively. Compared with previous studies, QYr.AYH-5BL may be a new QTL, while QYr.AYH-7DS may be Yr18. Haplotype analysis revealed that QYr.AYH-5BL is likely present in 6.2% of the 323 surveyed Chinese wheat landraces. The Kompetitive allele specific PCR (KASP) markers for QYr.AYH-5BL were developed by the linked SNP markers to successfully confirm the effects of the QTL in a validation population derived from a residual heterozygous line, and were further assessed in 38 Chinese wheat landraces and 92 cultivars. Our results indicated that QYr.AYH-5BL with linked KASP markers should have potential value for marker-assisted selection to improve stripe rust resistance in breeding programs.
Li Long; Fangjie Yao; Miss Fangnian Guan; Yu-Kun Cheng; Miss Luyao Duan; Xuyang Zhao; Hao Li; Zhien Pu; Wei Li; Qian-Tao Jiang; Yuming Wei; Jian Ma; Houyang Kang; Shoufen Dai; Pengfei Qi; Qiang Xu; Mei Deng; You-Liang Zheng; Yunfeng Jiang; Guoyue Chen. A stable QTL on Chromosome 5BL Combined with Yr18 Conferring High-level Adult-plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong. Phytopathology® 2021, 1 .
AMA StyleLi Long, Fangjie Yao, Miss Fangnian Guan, Yu-Kun Cheng, Miss Luyao Duan, Xuyang Zhao, Hao Li, Zhien Pu, Wei Li, Qian-Tao Jiang, Yuming Wei, Jian Ma, Houyang Kang, Shoufen Dai, Pengfei Qi, Qiang Xu, Mei Deng, You-Liang Zheng, Yunfeng Jiang, Guoyue Chen. A stable QTL on Chromosome 5BL Combined with Yr18 Conferring High-level Adult-plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong. Phytopathology®. 2021; ():1.
Chicago/Turabian StyleLi Long; Fangjie Yao; Miss Fangnian Guan; Yu-Kun Cheng; Miss Luyao Duan; Xuyang Zhao; Hao Li; Zhien Pu; Wei Li; Qian-Tao Jiang; Yuming Wei; Jian Ma; Houyang Kang; Shoufen Dai; Pengfei Qi; Qiang Xu; Mei Deng; You-Liang Zheng; Yunfeng Jiang; Guoyue Chen. 2021. "A stable QTL on Chromosome 5BL Combined with Yr18 Conferring High-level Adult-plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong." Phytopathology® , no. : 1.
Background Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant cultivars are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions. In addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under a controlled greenhouse environment. Results Seventeen accessions showed stable high-level resistance to stripe rust across all environments in the field tests. Four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers that covered the entire genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, whereas four and one QTL conferring seedling and adult-plant resistance, respectively, were mapped distantly from previously reported stripe rust resistance genes or QTL and thus may be novel resistance loci. Conclusions Our results provided an integrated overview of stripe rust resistance resources in a wheat landrace diversity panel from the southern autumn-sown spring wheat zone of China. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.
Yuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. BMC Genomics 2021, 22, 1 -15.
AMA StyleYuqi Wang, Can Yu, Yukun Cheng, Fangjie Yao, Li Long, Yu Wu, Jing Li, Hao Li, Jirui Wang, Qiantao Jiang, Wei Li, Zhien Pu, Pengfei Qi, Jian Ma, Mei Deng, Yuming Wei, Xianming Chen, Guoyue Chen, Houyang Kang, Yunfeng Jiang, Youliang Zheng. Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. BMC Genomics. 2021; 22 (1):1-15.
Chicago/Turabian StyleYuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. 2021. "Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone." BMC Genomics 22, no. 1: 1-15.
Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant cultivars are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions. In addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under a controlled greenhouse environment.Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments in the field tests. Four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers that covered the entire genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, whereas four and one QTL conferring seedling and adult-plant resistance, respectively, were mapped distantly from previously reported stripe rust resistance genes or QTL and thus may be novel resistance loci.Conclusions: Our results provided an integrated overview of stripe rust resistance resources in a wheat landrace diversity panel from the southern autumn-sown spring wheat zone of China. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.
Yuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. 2020, 1 .
AMA StyleYuqi Wang, Can Yu, Yukun Cheng, Fangjie Yao, Li Long, Yu Wu, Jing Li, Hao Li, Jirui Wang, Qiantao Jiang, Wei Li, Zhien Pu, Pengfei Qi, Jian Ma, Mei Deng, Yuming Wei, Xianming Chen, Guoyue Chen, Houyang Kang, Yunfeng Jiang, Youliang Zheng. Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. . 2020; ():1.
Chicago/Turabian StyleYuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. 2020. "Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone." , no. : 1.
Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant varieties are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions, in addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under controlled greenhouse conditions.Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments under field tests, while four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers covering the whole genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, while four and one QTL conferring seedling and adult-plant resistance respectively were mapped distantly from previously reported stripe rust resistance genes or QTL and may be novel resistance loci.Conclusions: Our results provided an integrated view of stripe rust resistance resources in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.
Yuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. 2020, 1 .
AMA StyleYuqi Wang, Can Yu, Yukun Cheng, Fangjie Yao, Li Long, Yu Wu, Jing Li, Hao Li, Jirui Wang, Qiantao Jiang, Wei Li, Zhien Pu, Pengfei Qi, Jian Ma, Mei Deng, Yuming Wei, Xianming Chen, Guoyue Chen, Houyang Kang, Yunfeng Jiang, Youliang Zheng. Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. . 2020; ():1.
Chicago/Turabian StyleYuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. 2020. "Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone." , no. : 1.
Backgrounds: Grain protein concentration (GPC), grain starch concentration (GSC), and wet gluten concentration (WGC) are complex traits that determine nutrient concentration, end-use quality, and yield in wheat. To identify the elite and stable loci or genomic regions conferring high GPC, GSC, and WGC, a genome-wide association study (GWAS) based on a mixed linear model (MLM) was performed using 55K single nucleotide polymorphism (SNP) array in a panel of 236 wheat accessions, including 160 commercial varieties and 76 landraces, derived from Sichuan Province, China. The panel was evaluated for GPC, GSC, and WGC at four different fields. Results: Phenotypic analysis showed variation in GPC, GSC, and WGC among the different genotypes and environments. GWAS identified 12 quantitative trait loci (QTL) (-log10(P) > 2.5) associated with these three quality traits in at least two environments and located on chromosomes 1B, 1D, 2A, 2B, 2D, 3B, 3D, 5D, and 7D; the phenotypic variation explained (PVE) by these QTL ranged from 4.2% to 10.7%. Among these, three, seven, and two QTL are associated with GPC, GSC, and WGC, respectively; five QTL (QGsc.sicau-1BL, QGsc.sicau-1DS, QGsc.sicau-2DL.1, QGsc.sicau-2DL.2, QWgc.sicau-5DL) were defined potentially novel Compared with the previously reported QTLs/genes by linkage or association mapping, 5 QTLs (QGsc.sicau-1BL, QGsc.sicau-1DS, QGsc.sicau-2DL.1, QGsc.sicau-2DL.2, QWgc.sicau-5DL) were potentially novel. Furthermore, 21 presumptive candidate genes, which are involved in the metabolism or transportation of all kinds of carbohydrates, photosynthesis, programmed cell death, the balance of abscisic acid and ethylene, within these potentially novel genomic regions were predicted. Conclusions: This study provided new genetic resources and valuable genetic information of nutritional quality to broaden the genetic background and laid the molecular foundation for marker-assisted selection in wheat quality breeding.
Zhien Pu; Xueling Ye; Yang Li; Zehou Liu; Bingxin Shi; Shoufen Dai; Yunfeng Jiang; Wei Li; Qiantao Jiang; Guoyue Chen; Yuming Wei; Youliang Zheng. Genome-Wide association study identifies new elements on the genetic basis of quality-related traits in wheat across multiple environments. 2020, 1 .
AMA StyleZhien Pu, Xueling Ye, Yang Li, Zehou Liu, Bingxin Shi, Shoufen Dai, Yunfeng Jiang, Wei Li, Qiantao Jiang, Guoyue Chen, Yuming Wei, Youliang Zheng. Genome-Wide association study identifies new elements on the genetic basis of quality-related traits in wheat across multiple environments. . 2020; ():1.
Chicago/Turabian StyleZhien Pu; Xueling Ye; Yang Li; Zehou Liu; Bingxin Shi; Shoufen Dai; Yunfeng Jiang; Wei Li; Qiantao Jiang; Guoyue Chen; Yuming Wei; Youliang Zheng. 2020. "Genome-Wide association study identifies new elements on the genetic basis of quality-related traits in wheat across multiple environments." , no. : 1.
Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant varieties are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions, in addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under controlled greenhouse conditions. Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments under field tests, while four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers covering the whole genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, while four and one QTL conferring seedling and adult-plant resistance respectively were mapped distantly from previously reported stripe rust resistance genes or QTL and may be novel resistance loci. Conclusions: Our results provided an integrated view of stripe rust resistance resources in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.
Yuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. 2020, 1 .
AMA StyleYuqi Wang, Can Yu, Yukun Cheng, Fangjie Yao, Li Long, Yu Wu, Jing Li, Hao Li, Jirui Wang, Qiantao Jiang, Wei Li, Zhien Pu, Pengfei Qi, Jian Ma, Mei Deng, Yuming Wei, Xianming Chen, Guoyue Chen, Houyang Kang, Yunfeng Jiang, Youliang Zheng. Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. . 2020; ():1.
Chicago/Turabian StyleYuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. 2020. "Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone." , no. : 1.
A novel and major QTL for the effective tiller number was identified on chromosomal arm 1BL and validated in two genetic backgrounds The effective tiller number (ETN) substantially influences plant architecture and the wheat yield improvement. In this study, we constructed a genetic map of the 2SY (20828/SY95-71) recombinant inbred line population based on the Wheat 55K array as well as the simple sequence repeat (SSR) and Kompetitive Allele Specific PCR (KASP) markers. A comparison between the genetic and physical maps indicated the marker positions were consistent in the two maps. Additionally, we identified seven tillering-related quantitative trait locus (QTLs), including Qetn-sau-1B.1, which is a major QTL localized to a 6.17-cM interval flanked by markers AX-89635557 and AX-111544678 on chromosome 1BL. The Qetn-sau-1B.1 QTL was detected in eight environments and explained 12.12–55.71% of the phenotypic variance. Three genes associated with the ETN were detected in the physical interval of Qetn-sau-1B.1. We used a tightly linked KASP marker, KASP-AX-110129912, to further validate this QTL in two other populations with different genetic backgrounds. The results indicated that Qetn-sau-1B.1 significantly increased the ETN by up to 23.5%. The results of this study will be useful for the precise mapping and cloning of Qetn-sau-1B.1.
Jiajun Liu; Huaping Tang; Xiangru Qu; Hang Liu; Cong Li; Yang Tu; Shuiqing Li; Ahsan Habib; Yang Mu; Shoufeng Dai; Mei Deng; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Guangdeng Chen; Wei Li; Yunfeng Jiang; Yuming Wei; Xiujin Lan; Youliang Zheng; Jian Ma. A novel, major, and validated QTL for the effective tiller number located on chromosome arm 1BL in bread wheat. Plant Molecular Biology 2020, 104, 173 -185.
AMA StyleJiajun Liu, Huaping Tang, Xiangru Qu, Hang Liu, Cong Li, Yang Tu, Shuiqing Li, Ahsan Habib, Yang Mu, Shoufeng Dai, Mei Deng, Qiantao Jiang, Yaxi Liu, Guoyue Chen, Jirui Wang, Guangdeng Chen, Wei Li, Yunfeng Jiang, Yuming Wei, Xiujin Lan, Youliang Zheng, Jian Ma. A novel, major, and validated QTL for the effective tiller number located on chromosome arm 1BL in bread wheat. Plant Molecular Biology. 2020; 104 (1-2):173-185.
Chicago/Turabian StyleJiajun Liu; Huaping Tang; Xiangru Qu; Hang Liu; Cong Li; Yang Tu; Shuiqing Li; Ahsan Habib; Yang Mu; Shoufeng Dai; Mei Deng; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Guangdeng Chen; Wei Li; Yunfeng Jiang; Yuming Wei; Xiujin Lan; Youliang Zheng; Jian Ma. 2020. "A novel, major, and validated QTL for the effective tiller number located on chromosome arm 1BL in bread wheat." Plant Molecular Biology 104, no. 1-2: 173-185.
Chinese endemic wheat, comprising Tibetan semi-wild wheat (Triticum aestivum ssp. tibetanum), Yunnan hulled wheat (T. aestivum ssp. yunnanense), and Xinjiang rice wheat (T. petropavlovskyi), are genetically and morphologically unique. To examine the adult plant resistance to stripe rust among Chinese endemic wheat germplasms, a panel of 213 accessions was inoculated with mixed virulent races of wheat stripe rust (Puccinia striiformis f. sp. tritici) in four different field environments. Four traits associated with stripe rust resistance, infection type, final disease severity, disease index, and area under the disease progress curve, were used to evaluate the accessions. The phenotypic datasets were used for 55K single-nucleotide polymorphism (SNP) array-based genome-wide association studies to identify effective resistance loci. Eighty-nine accessions with stable resistance were identified in at least three of the four environments by phenotypic evaluation. Eleven markers located on chromosomes 1A, 2B, 5A, 5D, 7B, and 7D by the genome-wide association studies analysis showed significant associations with at least two resistance-associated traits in two of the environments. These loci, corresponding to seven genomic regions based on linkage disequilibrium decay distance, explained 9.3 to 26.0% of the total phenotypic variation. Five quantitative trait loci (QTLs) on chromosomes 1A, 2B, 7B, and 7D overlapped or were in close proximity to previously reported QTLs based on the consensus and physical maps using the reference sequence of bread wheat (IWGSC RefSeq v1.0). The other two QTLs were potential novel QTLs given their physical positions. Haplotype variants of QTL QYr.sicau-2BS showed subspecies-specific inheritance of the stripe rust resistance locus. Resistant loci among Chinese endemic wheat germplasms could be introduced into common wheat cultivars, and the high-confidence SNP markers will aid in marker-assisted selection in breeding for stripe rust disease resistance.
Jing Li; Yunfeng Jiang; Fangjie Yao; Li Long; Yuqi Wang; Yu Wu; Hao Li; Jirui Wang; Qiantao Jiang; Houyang Kang; Wei Li; Pengfei Qi; Jian Ma; Zhien Pu; Shoufen Dai; Yuming Wei; Youliang Zheng; Guoyue Chen. Genome-Wide Association Study Reveals the Genetic Architecture of Stripe Rust Resistance at the Adult Plant Stage in Chinese Endemic Wheat. Frontiers in Plant Science 2020, 11, 1 .
AMA StyleJing Li, Yunfeng Jiang, Fangjie Yao, Li Long, Yuqi Wang, Yu Wu, Hao Li, Jirui Wang, Qiantao Jiang, Houyang Kang, Wei Li, Pengfei Qi, Jian Ma, Zhien Pu, Shoufen Dai, Yuming Wei, Youliang Zheng, Guoyue Chen. Genome-Wide Association Study Reveals the Genetic Architecture of Stripe Rust Resistance at the Adult Plant Stage in Chinese Endemic Wheat. Frontiers in Plant Science. 2020; 11 ():1.
Chicago/Turabian StyleJing Li; Yunfeng Jiang; Fangjie Yao; Li Long; Yuqi Wang; Yu Wu; Hao Li; Jirui Wang; Qiantao Jiang; Houyang Kang; Wei Li; Pengfei Qi; Jian Ma; Zhien Pu; Shoufen Dai; Yuming Wei; Youliang Zheng; Guoyue Chen. 2020. "Genome-Wide Association Study Reveals the Genetic Architecture of Stripe Rust Resistance at the Adult Plant Stage in Chinese Endemic Wheat." Frontiers in Plant Science 11, no. : 1.
Emerging evidence demonstrates that lncRNAs participate in various developmental processes in plants via post-transcription regulation. However, few lncRNAs have been identified as regulators of tiller development in wheat (Triticum aestivum L.). In this study, high-throughput ribosomal depleted RNA sequencing was performed on the tillering nodes of two pairs of near-isogenic lines that differed only in the tillering trait. We identified 5399 lncRNA transcripts using bioinformational analyses. KEGG pathway analysis revealed 74 common differentially expressed lncRNAs substantially enriched in photosynthesis-related, phenylpropanoid biosynthesis, phosphatidylinositol signaling, brassinosteroid biosynthesis, zeatin biosynthesis, and carotenoid biosynthesis pathways. Detailed functional annotations of target genes were used to identify 27 tillering-associated lncRNAs. Among these, 10 were in photosynthesis-related pathways; 15 were in secondary metabolite pathways; and 8 were in plant hormone pathways, with 6 enriched in two kinds of pathways. These findings contribute to identifying tillering-associated lncRNAs in wheat and enable further investigation into the functions and roles of key candidate lncRNAs, and more experimental evidence was also needed if breeders wanted to utilize these candidate lncRNAs in wheat crop yield improvement in the future.
Wanlin Zhou; Haoran Shi; Zhiqiang Wang; Yueting Zhao; Xiangjian Gou; Caixia Li; Guangdeng Chen; Shihang Liu; Mei Deng; Jian Ma; Youliang Zheng; Yuming Wei; Yaxi Liu. Identification of lncRNAs involved in wheat tillering development in two pairs of near-isogenic lines. Functional & Integrative Genomics 2020, 20, 669 -679.
AMA StyleWanlin Zhou, Haoran Shi, Zhiqiang Wang, Yueting Zhao, Xiangjian Gou, Caixia Li, Guangdeng Chen, Shihang Liu, Mei Deng, Jian Ma, Youliang Zheng, Yuming Wei, Yaxi Liu. Identification of lncRNAs involved in wheat tillering development in two pairs of near-isogenic lines. Functional & Integrative Genomics. 2020; 20 (5):669-679.
Chicago/Turabian StyleWanlin Zhou; Haoran Shi; Zhiqiang Wang; Yueting Zhao; Xiangjian Gou; Caixia Li; Guangdeng Chen; Shihang Liu; Mei Deng; Jian Ma; Youliang Zheng; Yuming Wei; Yaxi Liu. 2020. "Identification of lncRNAs involved in wheat tillering development in two pairs of near-isogenic lines." Functional & Integrative Genomics 20, no. 5: 669-679.
Background:Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe-rust resistance genes and cultivation of resistant varieties are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe-rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions, in addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under controlled greenhouse conditions. Results:Seventeen accessions showed stable high-level resistance to stripe rust across all environments under field tests,while four accessions showed resistance to the Pst races CYR32 and CYR34 at the all-stage.Combining phenotypic data from the field and greenhouse trials with 6404 markers covering the whole genome, we detected 17quantitative trait locus(QTLs) on 11 chromosomes for IT associated with seedling resistance and 15 QTLs on seven chromosomes for IT, final disease severityor AUDPC associated with adult-plant resistance. Four stable QTLs detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe-rust resistance in wheat, 29 QTLs overlapped with previously reported genes or QTLs, while two and oneQTLs conferring seedling and adult-plant resistancerespectively were mapped distantly from previously reported stripe rust resistance genes or QTLs and may be novel resistantloci. Conclusions:Our results provided an integrated view of stripe rust resistance resources in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone.The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.
Yuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. Genome-wide Association Mapping Reveals Potential Novel Loci Controlling Stripe-rust Resistance in a Chinese Wheat Landrace Diversity Panel From the Southern Autumn-sown Spring Wheat Zone. 2020, 1 .
AMA StyleYuqi Wang, Can Yu, Yukun Cheng, Fangjie Yao, Li Long, Yu Wu, Jing Li, Hao Li, Jirui Wang, Qiantao Jiang, Wei Li, Zhien Pu, Pengfei Qi, Jian Ma, Mei Deng, Yuming Wei, Xianming Chen, Guoyue Chen, Houyang Kang, Yunfeng Jiang, Youliang Zheng. Genome-wide Association Mapping Reveals Potential Novel Loci Controlling Stripe-rust Resistance in a Chinese Wheat Landrace Diversity Panel From the Southern Autumn-sown Spring Wheat Zone. . 2020; ():1.
Chicago/Turabian StyleYuqi Wang; Can Yu; Yukun Cheng; Fangjie Yao; Li Long; Yu Wu; Jing Li; Hao Li; Jirui Wang; Qiantao Jiang; Wei Li; Zhien Pu; Pengfei Qi; Jian Ma; Mei Deng; Yuming Wei; Xianming Chen; Guoyue Chen; Houyang Kang; Yunfeng Jiang; Youliang Zheng. 2020. "Genome-wide Association Mapping Reveals Potential Novel Loci Controlling Stripe-rust Resistance in a Chinese Wheat Landrace Diversity Panel From the Southern Autumn-sown Spring Wheat Zone." , no. : 1.
We identified and validated two stable grain filling rate (GFR) quantitative trait loci (QTL) in wheat that positively influenced several yield-related traits. Among them, QGfr.sicau-7D.1 was a novel GFR QTL. The grain filling rate (GFR) plays a crucial role in determining grain yield. To advance the current understanding of the genetic characteristics underlying the GFR in common wheat, three recombinant inbred line populations were used to map and validate GFR quantitative trait loci (QTL). Using a high-density genetic linkage map, 10 GFR QTL were detected. They were located on chromosomes 2D, 4A, 4B, 5B, 6D, 7A and 7D, explained 4.99–12.62% of the phenotypic variation. Two of them, QGfr.sicau-6D and QGfr.sicau-7D.1, were detected in all four environments tested and their genetic effect was validated by closely linked kompetitive allele specific PCR (KASP) markers in different genetic backgrounds. The effects of these two GFR QTL on other yield-related traits were also estimated. QGfr.sicau-6D had a significant positive influence (p < 0.01) on thousand kernel weight, kernel width, kernel volume, and kernel surface area. QGfr.sicau-7D.1 had a significant positive influence (p < 0.01) on thousand kernel weight and kernel length. Furthermore, QGfr.sicau-7D.1 was a completely novel QTL for GFR; several genes associated with grain growth and development were predicted in its physical interval. These results will facilitate molecular marker-assisted selection of wheat with high-confidence QTL for GFR and fine mapping of genes associated with GFR, thereby contributing to yield improvement.
Yu Lin; Xiaojun Jiang; Yang Tao; Xilan Yang; Zhiqiang Wang; Fangkun Wu; Shihang Liu; Caixia Li; Mei Deng; Jian Ma; Guangdeng Chen; Yuming Wei; Youliang Zheng; Yaxi Liu. Identification and validation of stable quantitative trait loci for grain filling rate in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics 2020, 133, 2377 -2385.
AMA StyleYu Lin, Xiaojun Jiang, Yang Tao, Xilan Yang, Zhiqiang Wang, Fangkun Wu, Shihang Liu, Caixia Li, Mei Deng, Jian Ma, Guangdeng Chen, Yuming Wei, Youliang Zheng, Yaxi Liu. Identification and validation of stable quantitative trait loci for grain filling rate in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics. 2020; 133 (8):2377-2385.
Chicago/Turabian StyleYu Lin; Xiaojun Jiang; Yang Tao; Xilan Yang; Zhiqiang Wang; Fangkun Wu; Shihang Liu; Caixia Li; Mei Deng; Jian Ma; Guangdeng Chen; Yuming Wei; Youliang Zheng; Yaxi Liu. 2020. "Identification and validation of stable quantitative trait loci for grain filling rate in common wheat (Triticum aestivum L.)." Theoretical and Applied Genetics 133, no. 8: 2377-2385.
Aegilops comosa and Ae. markgrafii are diploid progenitors of polyploidy species of Aegilops sharing M and C genomes, respectively. Transferring valuable genes/traits from Aegilops into wheat is an alternative strategy for wheat genetic improvement. The amphidiploids between diploid species of Aegilops and tetraploid wheat can act as bridges to overcome obstacles from direct hybridization and can be developed by the union of unreduced gametes. In this study, we developed seven Triticum turgidum – Ae. comosa and two T. turgidum – Ae. markgrafii amphidiploids. The unreduced gametes mechanisms, including first-division restitution (FDR) and single-division meiosis (SDM), were observed in triploid F1 hybrids of T. turgidum – Ae. comosa (STM) and T. turgidum – Ae. markgrafii (STC). Only FDR was observed in STC hybrids, whereas FDR or both FDR and SDM were detected in the STM hybrids. All seven pairs of M chromosomes of Ae. comosa and C chromosomes of Ae. markgrafii were distinguished by fluorescent in situ hybridization (FISH) probes pSc119.2 and pTa71 combinations with pTa-535 and (CTT)12/(ACT)7, respectively. Meanwhile, the chromosomes of tetraploid wheat and diploid Aegilops parents were distinguished by the same FISH probes. The amphidiploids possessed specific valuable traits such as multiple tillers, large seed size related traits, and stripe rust resistance that could be utilized in the genetic improvement of wheat.
Yuanyuan Zuo; Qin Xiang; Shou-Fen Dai; Zhongping Song; Tingyu Bao; Ming Hao; Lianquan Zhang; Gang Liu; Jian Li; Dengcai Liu D.C. Liu; Yu-Ming Wei; Youliang Zheng; Zehong Yan; Hao Ming. Development and characterization of Triticum turgidum – Aegilops comosa and T. turgidum – Ae. markgrafii amphidiploids. Genome 2020, 63, 263 -273.
AMA StyleYuanyuan Zuo, Qin Xiang, Shou-Fen Dai, Zhongping Song, Tingyu Bao, Ming Hao, Lianquan Zhang, Gang Liu, Jian Li, Dengcai Liu D.C. Liu, Yu-Ming Wei, Youliang Zheng, Zehong Yan, Hao Ming. Development and characterization of Triticum turgidum – Aegilops comosa and T. turgidum – Ae. markgrafii amphidiploids. Genome. 2020; 63 (5):263-273.
Chicago/Turabian StyleYuanyuan Zuo; Qin Xiang; Shou-Fen Dai; Zhongping Song; Tingyu Bao; Ming Hao; Lianquan Zhang; Gang Liu; Jian Li; Dengcai Liu D.C. Liu; Yu-Ming Wei; Youliang Zheng; Zehong Yan; Hao Ming. 2020. "Development and characterization of Triticum turgidum – Aegilops comosa and T. turgidum – Ae. markgrafii amphidiploids." Genome 63, no. 5: 263-273.
A completely developed and vigorous root system can provide a stable platform for aboveground plant organs. To identify loci controlling root traits that could be used in wheat (Triticum aestivum L.) breeding, 199 recombinant inbred lines were used to measure and analyze eight root traits. A total of 18 quantitative trait loci (QTL) located on chromosomes 1A, 2A, 2B, 2D, 4B, 4D, 6A, 7A, and 7B were identified. The phenotypic variation explained by these 18 QTL ranged from 3.27% to 11.75%, and the logarithm of odds scores ranged from 2.50 to 6.58. A comparison of physical intervals indicated several new QTL for root traits were identified. In addition, significant correlations between root and agronomic traits were detected and discussed. The results presented in this study, along with those of previous reports, suggest that chromosomes 2 and 7 likely play important roles in the growth and development of wheat roots.
Ting Li; Jian Ma; Yaya Zou; Guangdeng Chen; Pu-Yang Ding; Han Zhang; Cong-Cong Yang; Yang Mu; Huaping Tang; Yaxi Liu; Qiantao Jiang; Guo-Yue Chen; Peng-Fei Qi; Yu-Ming Wei; Youliang Zheng; Xiu-Jin Lan. Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat. Genome 2020, 63, 27 -36.
AMA StyleTing Li, Jian Ma, Yaya Zou, Guangdeng Chen, Pu-Yang Ding, Han Zhang, Cong-Cong Yang, Yang Mu, Huaping Tang, Yaxi Liu, Qiantao Jiang, Guo-Yue Chen, Peng-Fei Qi, Yu-Ming Wei, Youliang Zheng, Xiu-Jin Lan. Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat. Genome. 2020; 63 (1):27-36.
Chicago/Turabian StyleTing Li; Jian Ma; Yaya Zou; Guangdeng Chen; Pu-Yang Ding; Han Zhang; Cong-Cong Yang; Yang Mu; Huaping Tang; Yaxi Liu; Qiantao Jiang; Guo-Yue Chen; Peng-Fei Qi; Yu-Ming Wei; Youliang Zheng; Xiu-Jin Lan. 2020. "Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat." Genome 63, no. 1: 27-36.