Weedy rice (Oryza sativa L.) is one of the major issues of rice cultivation worldwide. In Italy, it infests about 70% of the total rice area. Different Weedy Rice populations can be distinguished based on variable morphological and physiological traits; however, little is known about genetic differentiation and origin of Italian weedy rice populations. The objective of this study was to genetically and morphologically characterize and compare different Italian weedy rice populations selected on the basis of different phenotypes. The main Italian rice territory was divided into 10 geographical areas in which 40 weedy rice populations were collected and grouped according to the awn traits. All the individuals of the populations were morphologically characterized according to plant and seed traits. Genetic characterization was performed using 19 SSR markers on all the collected accessions, and several rice cultivars, including some very old (late 19th century), nowadays are no longer cultivated. ANOVA showed that morphological plant and seed traits were significantly affected by the collection area and awnedness group. The importance of the awn morphology was also reflected in the Bayesian clustering where, despite a relatively low genetic diversity, the clusters displayed different awn types. An UPGMA dendrogram confirmed the clusters detected in STRUCTURE analysis and also revealed a grouping of certain old cultivars with the weedy rice, suggesting a common origin.

Weedy rice is a problematic weed that infests paddy fields worldwide. Differing populations, with varying physiological and morphological traits, characterize this weed. In particular, seed dormancy makes its control difficult. The objective of this study was to evaluate the germination behavior of five Italian weedy rice populations (two awnless, two awned, and one mucronate) after exposure of seeds to different field storage conditions (flooding, burial, and dry soil surface) during winter in two sites (Grugliasco and Vercelli, Italy). Seed samples were taken from each population, storage condition, and site, every 15 d for petri dish germinability testing. The two sites displayed slightly different germination patterns, which were probably due to the differing climatic conditions. One of the awned populations showed the highest (always exceeding 80%) and fastest germination percentage in all field conditions and sites, compared with the other four populations. Although flooding promoted germination in one awnless population, it delayed germination in two others (one awned and one awnless), attaining only 20% germination after more than 100 d. In all populations, burial delayed germination, whereas seed placement on the dry soil surface enhanced it. Our study indicated that autumn tillage that promotes weedy rice seed burial should be discouraged; spring tillage that exposes seeds to the soil surface and cause their depletion should be encouraged. The tested technique of winter flooding can also improve weedy rice control, despite its varying efficacy among populations. Cycles of flooding and drying followed by spring tillage might improve weedy rice seed control.

Weeds that have become resistant to herbicides may threaten rice production. Rice cultivation is mainly carried out in coastal and river delta areas that often suffer salinity problems. The aims of this study were to evaluate the effects of salinity upon germination and the root and shoot seedling growth of Italian weedy rice and cultivated rice (Oryza sativa), and to find a possible correlation between salinity and herbicide resistance. Seed germination tests were conducted in Petri dishes on four imazamox-sensitive and one resistant weedy rice populations and two rice varieties: Baldo (conventional) and CL80 (imidazolinone-resistant Clearfield^® variety). Different salt concentrations were tested: 0, 50, 100, 150, 200, 250, 300, 350 and 400 mM NaCl. Germination percentage, germination speed, seedling root and shoot length were affected by increasing the salt concentration in all tested populations and varieties. The germination percentage was in general more affected in resistant weedy rice and CL80. In resistant weedy rice this was partially compensated by a faster germination up to 100 mM. In terms of seedling root and shoot length, CL80 and Baldo showed the highest tolerance to salt; resistant weedy rice was not able to produce seedling roots and shoots at concentrations > 300 mM.