Hepatotoxic microcystins (MCs) are the most widespread class of cyanotoxins and the one that has most often been implicated in cyanobacterial toxicosis. One of the main challenges in studying and monitoring MCs is the great structural diversity within the class. The full chemical structure of the first MC was elucidated in the early 1980s and since then, the number of reported structural analogues has grown steadily and continues to do so, thanks largely to advances in analytical methodology. The structures of some of these analogues have been definitively elucidated after chemical isolation using a combination of techniques including nuclear magnetic resonance, amino acid analysis, and tandem mass spectrometry (MS/MS). Others have only been tentatively identified using liquid chromatography-MS/MS without chemical isolation. An understanding of the structural diversity of MCs, the genetic and environmental controls for this diversity and the impact of structure on toxicity are all essential to the ongoing study of MCs across several scientific disciplines. However, because of the diversity of MCs and the range of approaches that have been taken for characterizing them, comprehensive information on the state of knowledge in each of these areas can be challenging to gather. We have conducted an in-depth review of the literature surrounding the identification and toxicity of known MCs and present here a concise review of these topics. At present, at least 279 MCs have been reported and are tabulated here. Among these, about 20% (55 of 279) appear to be the result of chemical or biochemical transformations of MCs that can occur in the environment or during sample handling and extraction of cyanobacteria, including oxidation products, methyl esters, or post-biosynthetic metabolites. The toxicity of many MCs has also been studied using a range of different approaches and a great deal of variability can be observed between reported toxicities, even for the same congener. This review will help clarify the current state of knowledge on the structural diversity of MCs as a class and the impacts of structure on toxicity, as well as to identify gaps in knowledge that should be addressed in future research.

The fresh fruit agricultural and distribution sector is faced with risks and uncertainties from climate change, water scarcity, land-use increase for industrial and urban development, consumer behavior, and price volatility. The planning framework for production and distribution is highly complex as a result. Mathematical models have been developed over the decades to deal with this complexity. With improvements in both processor speed and memory, these models are becoming increasingly sophisticated. This review focuses on the recent progress in mathematically based decision making to account for uncertainties in the fresh fruit supply chain. The models in the literature are mostly based on linear and mixed integer programming and involve variants such as stochastic programming and robust optimization. The functional areas of application include planting, harvest optimization, logistics and distribution. The perishability of the fresh fruit supply chain is an important issue as is the cycle time of cultivation and harvest.

We present an optimization model for dragon fruit plantations in Vietnam. The timing of cultivating and harvesting decisions are taken into account as the dragon fruit plant has an approximately ten-year life cycle with maximum average yield in the fourth year. Another consideration also included is the prevalence of forward-buying contracts with locked-in prices. The dragon fruit supply chain faces several difficulties as yield, price, and demand are highly sensitive to weather conditions and global uncertainty factors. The risk factors in the dragon fruit supply chain also depend on species—for example, the red varieties, while more profitable than the white varieties, also have higher export risk because they are subject to global prices and adverse geopolitical conditions.

This chapter will present an overview of cyanobacterial harmful algal blooms (cyanoHABs) and biotic and abiotic factors, as well as various aspects associated with these worldwide ecological bursts. The exact causes of the cyanoHABs are still not well defined, but eutrophication and climate change (temperature increase, light intensity variation, etc.) are the two assumed main factors that may promote the proliferation and expansion of cyanobacterial blooms. However, these premises need to be profoundly investigated as the optimal combination of all factors such as increased nutrient loading, physiological characteristics of cyanobacterial species, and climate effects which could lead to the blooming pattern will require robust modeling approaches to predict the phenomena. Negative issues associated with cyanoHABs are diverse including the toxic products (cyanotoxins) released by certain taxa which can damage the health of humans and animal habitats around the related watershed as well as generate a huge water quality problem for aquatic industries.

Strawberry is an important fruit crop in Canada but powdery mildew (PM) results in about 30–70% yield loss. Detection of PM through an image texture-based system is beneficial, as it identifies the symptoms at an earlier stage and reduces labour intensive manual monitoring of crop fields. This paper presents an image texture-based disease detection algorithm using supervised classifiers. Three sites were selected to collect the leaf image data in Great Village, Nova Scotia, Canada. Images were taken under an artificial cloud condition with a Digital Single Lens Reflex (DSLR) camera as red-green-blue (RGB) raw data throughout 2017–2018 summer. Three supervised classifiers, including artificial neural networks (ANN), support vector machine (SVM), and k-nearest neighbors (kNN) were evaluated for disease detection. A total of 40 textural features were extracted using a colour co-occurrence matrix (CCM). The collected feature data were normalized, then used for training and internal, external and cross-validations of developed classifiers. Results of this study revealed that the highest overall classification accuracy was 93.81% using the ANN classifier and lowest overall accuracy was 78.80% using the kNN classifier. Results identified the ANN classifier disease detection having a lower Root Mean Square Error (RMSE) = 0.004 and Mean Absolute Error (MAE) = 0.003 values with 99.99% of accuracy during internal validation and 87.41%, 88.95% and 95.04% of accuracies during external validations with three different fields. Overall results demonstrated that an image texture-based ANN classifier was able to classify PM disease more accurately at early stages of disease development.

Managing uncertainties and risks is always a difficult but fascinating task in fresh fruit supply chains, especially when dealing with the strategy for the production and conveyance of fresh fruit in Vietnam. Following the COVID-19 outbreak, the confluence of economic recession and persistent adverse weather conditions has exacerbated challenges faced by dragon fruit cultivators. This research investigates a two-stage stochastic programming (TSSP) approach which is developed and served as a valuable tool for analyzing uncertainties, optimizing operations, and managing risks in the fresh fruit industry, ultimately contributing to the sustainability and resilience of supply chains in the agricultural sector. A prototype is provided to illustrate the complex and dynamic nature of dragon fruit cultivation and consumption in Vietnam. Data on the selling prices of dragon fruit were collected from several sources between 2013 and 2022 in Binh Thuan Province, Vietnam. The results were obtained from the model by using three different approaches in order of their versatility and efficacy: (1) Scenario tree generation; (2) Sample average approximation; (3) Chance-constrained programming.