In the past decades, considerable attention has been directed toward anaerobic digestion (AD), which is an effective biological process for converting diverse organic wastes into biogas, volatile fatty acids (VFAs), biohydrogen, etc. The microbial bioprocessing takes part during AD is of substantial significance, and one of the crucial approaches for the deep and adequate understanding and manipulating it toward different products is process microbiology. Due to highly complexity of AD microbiome, it is critically important to study the involved microorganisms in AD. In recent years, in addition to traditional methods, novel molecular techniques and meta-omics approaches have been developed which provide accurate details about microbial communities involved AD. Better understanding of process microbiomes could guide us in identifying and controlling various factors in both improving the AD process and diverting metabolic pathway toward production of selective bio-products. This review covers various platforms of AD process that results in different final products from microbiological point of view. The review also highlights distinctive interactions occurring among microbial communities. Furthermore, assessment of these communities existing in the anaerobic digesters is discussed to provide more insights into their structure, dynamics, and metabolic pathways. Moreover, the important factors affecting microbial communities in each platform of AD are highlighted. Finally, the review provides some recent applications of AD for the production of novel bio-products and deals with challenges and future perspectives of AD.
Food waste (FW) management by biological process is more attractive and eco-friendly approach than thermo-chemical conversion or landfilling. However, FW composition and physico-chemical and biological characteristics affect the overall biological process in terms of product yield and degradation rate. To overcome this major bottle-neck, the pretreatment of FW is proposed. Therefore this review aims to provide a comprehensive summary of the importance of pretreatment of FW with respect to FW management by anaerobic digestion (AD) and dark fermentation (DF). It also reviews the existing knowledge gaps and future research perspectives for better integration of FW pretreatments for AD and DF, which should include (i) the preservation of carbon mass through freeze and thaw, or drying; and (ii) improve the carbon accessibility through particle size reduction and thermal pretreatments for high-rate bioenergy recovery.