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Dr. Helene Carrere
LBE, INRA Institut National de La Recherche Agronomique, Montpellier Université, Avenue des etangs, 11100 Narbonne, France

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0 Anaerobic Digestion
0 sludge
0 lignocellulosic biomass
0 dark fermentation
0 Physico–chemical and biological pretreatments

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Anaerobic Digestion
sludge
lignocellulosic biomass
dark fermentation
Fatty residues
Physico–chemical and biological pretreatments
Agricultural residues

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Journal article
Published: 13 March 2021 in Bioresource Technology
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The aim of this work was to study an innovative alkaline process on two cover crops. CaO load of 60 g.kgTS−1 was implemented to combine the functions of storage and pretreatment. Lab-scale reactors were monitored for 180 days to assess the effect of this process on the physico-chemical properties of the biomass. From the first days, pH was not maintained in an alkaline zone and microbial fermentation activity was observed with the degradation of available carbohydrates and production of metabolites, CO2 and H2. High butyric acid accumulation was observed and mass losses of 18.1% and 9.0% of initial VS occurred for oat and rye, respectively. However, no methane potential loss was recorded in the short and long term and the crops were efficiently preserved. The pretreatment had no major impact on fiber solubilization, and no increase in BMP was obtained, which was attributed to the short duration of the alkaline conditions.

ACS Style

C. Van Vlierberghe; R. Escudié; N. Bernet; S. Frédéric; H. Carrere. Long term alkaline storage and pretreatment process of cover crops for anaerobic digestion. Bioresource Technology 2021, 330, 124986 .

AMA Style

C. Van Vlierberghe, R. Escudié, N. Bernet, S. Frédéric, H. Carrere. Long term alkaline storage and pretreatment process of cover crops for anaerobic digestion. Bioresource Technology. 2021; 330 ():124986.

Chicago/Turabian Style

C. Van Vlierberghe; R. Escudié; N. Bernet; S. Frédéric; H. Carrere. 2021. "Long term alkaline storage and pretreatment process of cover crops for anaerobic digestion." Bioresource Technology 330, no. : 124986.

Journal article
Published: 12 February 2021 in Energies
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Fast development of centralized agricultural biogas plants leads to high amounts of digestate production. The treatment and disposal of liquid fractions after on-site digestate solid–liquid separation remains problematic due to their high organic, nutrient and aromatic contents. This work aims to study the variability of the remaining compounds in the digestate liquid fractions in relation to substrate origin, process parameters and solid–liquid separation techniques. Twenty-nine digestates from full-scale codigestion biogas plants and one waste activated sludge (WAS) digestate were collected and characterized. This study highlighted the combined effect of the solid–liquid separation process and the anaerobic digestion feedstock on the characteristics of liquid fractions of digestates. Two major clusters were found: (1) liquid fractions from high efficiency separation process equipment (e.g., centrifuge and others with addition of coagulant, flocculent or polymer) and (2) liquid fractions from low efficiency separation processes (e.g., screw press, vibrating screen and rotary drum), in this latter case, the concentration of chemical oxygen demand (COD) was associated with the proportion of cow manure and energy crops at biogas plant input. Finally, SUVA254, an indicator for aromatic molecule content and the stabilization of organic matter, was associated with the hydraulic retention time (HRT).

ACS Style

Afifi Akhiar; Felipe Guilayn; Michel Torrijos; Audrey Battimelli; Abd Shamsuddin; Hélène Carrère. Correlations between the Composition of Liquid Fraction of Full-Scale Digestates and Process Conditions. Energies 2021, 14, 971 .

AMA Style

Afifi Akhiar, Felipe Guilayn, Michel Torrijos, Audrey Battimelli, Abd Shamsuddin, Hélène Carrère. Correlations between the Composition of Liquid Fraction of Full-Scale Digestates and Process Conditions. Energies. 2021; 14 (4):971.

Chicago/Turabian Style

Afifi Akhiar; Felipe Guilayn; Michel Torrijos; Audrey Battimelli; Abd Shamsuddin; Hélène Carrère. 2021. "Correlations between the Composition of Liquid Fraction of Full-Scale Digestates and Process Conditions." Energies 14, no. 4: 971.

Journal article
Published: 03 February 2021 in Energy Conversion and Management
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This article aims at providing insight into the recirculation of (post-treated) solid digestate (SD) within digesters. Such a practice has been further identified as promising for anaerobically digesting recalcitrant feedstocks and for improving the energy efficiency of continuously stirred tank reactor (CSTR) agricultural biogas plants. Firstly, implementation strategies and the potential impact of SD direct recirculation on five CSTR biogas plants were investigated. According to the selected strategy, results indicated that plant methane production could rise by 0.6 to 6.3% or that a potential feedstock shortage of 64 to 1431 tons/year could be compensated. Secondly, the relevance of additional post-treatments for improving these initial results was evaluated. Thermo-chemical post-treatments successfully increased SD biodegradability by 30 to 46% although their costs were not compensated by additional methane production. Short-term aerobic post-treatments failed in increasing SD biodegradability (up to 21% loss in biomethane potential). Hence, at full scale, a quick and direct recirculation of SD excluding any post-treatment appears to be the optimal condition to apply. Finally, conditions for the full-scale implementation of direct SD recirculation were theoretically studied. This practice has proved to increase the solid retention time by 11 to 38% and the plant total solid content by 6 to 20%. Thus, the critical point for its implementation should be the capacity of the plant mixing system to handle such an increase in solids. The relevance of SD recirculation needs to be determined on a case-by-case basis. Consequently, for the first time, this article provides a framework where the conditions can be identified for direct SD recirculation to be a relevant digestate management practice. Overall, this article demonstrates how direct SD recirculation can be a simple and low-cost mean for improving agricultural CSTR biogas plant efficiency. It also highlights the importance of achieving efficient digestate management in the biogas sector in order to reduce the costs of biogas production.

ACS Style

Ulysse Brémond; Aude Bertrandias; Raphaëlle de Buyer; Eric Latrille; Julie Jimenez; Renaud Escudié; Jean-Philippe Steyer; Nicolas Bernet; Hélène Carrere. Recirculation of solid digestate to enhance energy efficiency of biogas plants: Strategies, conditions and impacts. Energy Conversion and Management 2021, 231, 113759 .

AMA Style

Ulysse Brémond, Aude Bertrandias, Raphaëlle de Buyer, Eric Latrille, Julie Jimenez, Renaud Escudié, Jean-Philippe Steyer, Nicolas Bernet, Hélène Carrere. Recirculation of solid digestate to enhance energy efficiency of biogas plants: Strategies, conditions and impacts. Energy Conversion and Management. 2021; 231 ():113759.

Chicago/Turabian Style

Ulysse Brémond; Aude Bertrandias; Raphaëlle de Buyer; Eric Latrille; Julie Jimenez; Renaud Escudié; Jean-Philippe Steyer; Nicolas Bernet; Hélène Carrere. 2021. "Recirculation of solid digestate to enhance energy efficiency of biogas plants: Strategies, conditions and impacts." Energy Conversion and Management 231, no. : 113759.

Review article
Published: 11 November 2020 in Journal of Cleaner Production
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The future European “Green Deal” will set ambitious greenhouse gas emissions reduction by 2030. Reaching these targets will partly rely on the decarbonization of the gas sector and the development of the biomethane. In this context, this article aims to provide a vision of the European biogas sector development from 2020 towards 2030 and beyond. It appears that the biogas sector is facing a shift in its development paradigm. Indeed, this sector is still mainly dominated by a model based on energy crops, high feed-in tariffs and local electrical production via combined heat and power units. However, biogas sector is now moving towards a different model, where organic wastes, agricultural by-products as well as sequential crops are mainly used as feedstocks, biogas is upgraded to biomethane for various applications (transportation, chemical production, heat, etc.) and subsidy schemes are progressively reduced. Overall, current European policies are pushing the sector to increase its sustainability and to reduce biogas production costs. To answer these challenges, the main improvement tracks are identified and discussed. In the future, it is believed that the biogas sector should address these aspects to ensure its growth towards 2030 and beyond.

ACS Style

Ulysse Brémond; Aude Bertrandias; Jean-Philippe Steyer; Nicolas Bernet; Hélène Carrere. A vision of European biogas sector development towards 2030: Trends and challenges. Journal of Cleaner Production 2020, 287, 125065 .

AMA Style

Ulysse Brémond, Aude Bertrandias, Jean-Philippe Steyer, Nicolas Bernet, Hélène Carrere. A vision of European biogas sector development towards 2030: Trends and challenges. Journal of Cleaner Production. 2020; 287 ():125065.

Chicago/Turabian Style

Ulysse Brémond; Aude Bertrandias; Jean-Philippe Steyer; Nicolas Bernet; Hélène Carrere. 2020. "A vision of European biogas sector development towards 2030: Trends and challenges." Journal of Cleaner Production 287, no. : 125065.

Journal article
Published: 06 July 2020 in Science of The Total Environment
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This study aims at investigating how organic waste co-digestion coupled with alkaline pretreatment can impact the methane production and agronomic value of produced digestates. For this purpose, sludge alone and mixed with olive pomace or macroalgal residues were subjected to anaerobic digestion with and without alkaline pretreatment. In addition, co-digestion of pretreated sludge with raw substrates was also carried out and compared to the whole mixture pretreatment. KOH pretreatment enhanced methane production by 39%, 15% and 49% from sludge, sludge mixed with olive pomace and sludge mixed with macroalgal residues, respectively. The digestates were characterised according to their physico-chemical and agronomic properties. They were then applied as biofertilizers for tomato growth during the first vegetative stage (28 days of culture). Concentrations in chlorophyll a and carotenoids in tomato plants, following sludge digestate addition, rose by 46% and 41% respectively. Sludge digestate enhanced tomato plant dry weight by 87%, while its nitrogen content increased by 90%. The impact of nitrogen and phosphorus contents in the digestate was strongest on tomato plant dry weight, thus explaining the efficiency of sludge digestate relative to other types of digestate. However, when methane production is considered, the combination of pre-treatment with co-digestion of macroalgal residues and sludge appears most beneficial for maximizing energy recovery and for biofertilizer generation.

ACS Style

Doha Elalami; Florian Monlau; Helene Carrere; Karima Abdelouahdi; Abdallah Oukarroum; Youssef Zeroual; Abdellatif Barakat. Effect of coupling alkaline pretreatment and sewage sludge co-digestion on methane production and fertilizer potential of digestate. Science of The Total Environment 2020, 743, 140670 .

AMA Style

Doha Elalami, Florian Monlau, Helene Carrere, Karima Abdelouahdi, Abdallah Oukarroum, Youssef Zeroual, Abdellatif Barakat. Effect of coupling alkaline pretreatment and sewage sludge co-digestion on methane production and fertilizer potential of digestate. Science of The Total Environment. 2020; 743 ():140670.

Chicago/Turabian Style

Doha Elalami; Florian Monlau; Helene Carrere; Karima Abdelouahdi; Abdallah Oukarroum; Youssef Zeroual; Abdellatif Barakat. 2020. "Effect of coupling alkaline pretreatment and sewage sludge co-digestion on methane production and fertilizer potential of digestate." Science of The Total Environment 743, no. : 140670.

Review
Published: 26 June 2020 in Waste and Biomass Valorization
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The increasing demand for a Thermal Hydrolysis Process (THP) to pretreat municipal sludge upstream Anaerobic Digestion (AD) opens the opportunity to further develop and optimise this technology. The optimal THP temperature remains unclear due to the production of refractory compounds at high temperature. A compilation of literature data was conducted to investigate the existence of a temperature sweet spot for the THP applied to municipal sludge. All related reports (n = 43) were included. The THP temperature range impact was assessed in the range of 100 °C–200 °C on four AD and dewatering performance indicators (CH4 production, Volatile Solid Reduction (VSR), Dewaterability (DW) and filtrate quality). Other parameters potentially affecting the performance indicators were also considered. These parameters include the type of sewage sludge and operational conditions related to THP and AD. The impact of all parameters on performance indicators was evaluated with a Kruskal–Wallis statistical test. For THP temperature optimisation, a pairwise comparison, using a Wilcoxon test, was made. A temperature optimum in the [140–160] °C range was proposed. It seemed to minimize the production of refractory compounds, while maximising AD and dewatering performances. It is noteworthy that above 160 °C, the concentration in refractory compounds and soluble COD increases sharply, thus leading to a potential deterioration of WWTP effluent quality. Optimal THP temperature range for an optimal outcome on both sludge and wastewater treatment lines.

ACS Style

Perrine Devos; Mathieu Haddad; Hélène Carrère. Thermal Hydrolysis of Municipal sludge: Finding the Temperature Sweet Spot: A Review. Waste and Biomass Valorization 2020, 12, 2187 -2205.

AMA Style

Perrine Devos, Mathieu Haddad, Hélène Carrère. Thermal Hydrolysis of Municipal sludge: Finding the Temperature Sweet Spot: A Review. Waste and Biomass Valorization. 2020; 12 (5):2187-2205.

Chicago/Turabian Style

Perrine Devos; Mathieu Haddad; Hélène Carrère. 2020. "Thermal Hydrolysis of Municipal sludge: Finding the Temperature Sweet Spot: A Review." Waste and Biomass Valorization 12, no. 5: 2187-2205.

Journal article
Published: 27 April 2020 in Waste Management
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The aim of this paper is to investigate the impact of pretreating macroalgal residue (MAR) from agar-agar extraction and its co-digestion with sewage sludge on methane production and the agronomic quality of the digestates produced. First, different pretreatments were assessed on BMP tests. Among milling technologies used, knife milling with a 4 mm-screen improved methane production by 25%. The MAR was then knife milled before alkaline, acid and thermal pretreatment. KOH pretreatment (5% TS basis, 25 °C for 2 days) led to the highest methane improvement. It was applied to semi-continuous anaerobic digestion and methane production achieved 237 Nml/gVS which was 20% higher than the control (198 Nml/gVS). In comparison to MAR mono-digestion, co-digestion with thickened activated sludge produced less methane (184 Nml/gVS) but reduced H2S emission by 91%. None of the digestates was toxic for the germination or growth of wheat and tomato plants. Particularly, co-digestion had the highest impact on tomato plant dry weight (+94% compared to soil alone) mainly due to the phosphorous brought by sludge. However, the impact of alkaline pretreatment on plant growth was not significant.

ACS Style

Doha Elalami; Florian Monlau; Helene Carrere; Karima Abdelouahdi; Céline Charbonnel; Abdallah Oukarroum; Youssef Zeroual; Abdellatif Barakat. Evaluation of agronomic properties of digestate from macroalgal residues anaerobic digestion: Impact of pretreatment and co-digestion with waste activated sludge. Waste Management 2020, 108, 127 -136.

AMA Style

Doha Elalami, Florian Monlau, Helene Carrere, Karima Abdelouahdi, Céline Charbonnel, Abdallah Oukarroum, Youssef Zeroual, Abdellatif Barakat. Evaluation of agronomic properties of digestate from macroalgal residues anaerobic digestion: Impact of pretreatment and co-digestion with waste activated sludge. Waste Management. 2020; 108 ():127-136.

Chicago/Turabian Style

Doha Elalami; Florian Monlau; Helene Carrere; Karima Abdelouahdi; Céline Charbonnel; Abdallah Oukarroum; Youssef Zeroual; Abdellatif Barakat. 2020. "Evaluation of agronomic properties of digestate from macroalgal residues anaerobic digestion: Impact of pretreatment and co-digestion with waste activated sludge." Waste Management 108, no. : 127-136.

Chapter
Published: 18 February 2020 in Handbook on Characterization of Biomass, Biowaste and Related By-products
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The aim of this chapter is to provide a description of the main methods used to assess biological transformation of biomass. It will address saccharification tests which account for the ability of biomass to release sugars during enzymatic hydrolysis. Biochemical methane potential (BMP) tests, which are widely used to assess anaerobic digestibility of biomass for the production of methane and BMP prediction by near infra-red spectroscopy will be presented. Biohydrogen potential (BHP), used to assess the ability to produce biohydrogen from biomass by dark fermentation will be described. Respirometry tests accounting for assessing aerobic degradability of biomass will be described. Incubation tests used to assess carbon and nitrogen degradation in soils will also be presented.

ACS Style

Hélène Carrère; Georgia Antonopoulou; Céline Druilhe; Eric Latrille; Gerasimos Lyberatos; Julie Jimenez; Ioanna Ntaikou; Konstantina Papadopoulou; Eric Trably; Anne Trémier. Methods to Assess Biological Transformation of Biomass. Handbook on Characterization of Biomass, Biowaste and Related By-products 2020, 641 -730.

AMA Style

Hélène Carrère, Georgia Antonopoulou, Céline Druilhe, Eric Latrille, Gerasimos Lyberatos, Julie Jimenez, Ioanna Ntaikou, Konstantina Papadopoulou, Eric Trably, Anne Trémier. Methods to Assess Biological Transformation of Biomass. Handbook on Characterization of Biomass, Biowaste and Related By-products. 2020; ():641-730.

Chicago/Turabian Style

Hélène Carrère; Georgia Antonopoulou; Céline Druilhe; Eric Latrille; Gerasimos Lyberatos; Julie Jimenez; Ioanna Ntaikou; Konstantina Papadopoulou; Eric Trably; Anne Trémier. 2020. "Methods to Assess Biological Transformation of Biomass." Handbook on Characterization of Biomass, Biowaste and Related By-products , no. : 641-730.

Journal article
Published: 23 January 2020 in Molecules
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The increasing population creates excess pressure on the plantation and production of fruits and vegetables across the world. Consumption demand during the whole year has made production compulsory in the covered production system (greenhouse). Production, harvesting, processing, transporting, and distribution chains of fruit and vegetables have resulted in a huge amount of wastes as an alternative source to produce biofuels. In this study, optimization of two pretreatment processes (NaOH and HCl assisted thermal) was investigated to enhance methane production from fruit and vegetable harvesting wastes (FVHW) that originate from greenhouses. NaOH concentration (0–6.5%), HCl concentration (0–5%), reaction temperature (60–100 °C), solid content (1–5%), time of reaction (1–5 h), and mixing speed (0–500 rpm) were chosen in a wide range of levels to optimize the process in a broad design boundary and to evaluate the positive and negative impacts of independent variables along with their ranges. Increasing NaOH and HCl concentrations resulted in higher COD solubilization but decreased the concentration of soluble sugars that can be converted directly into methane. Thus, the increasing concentrations of NaOH and HCl in the pretreatments have resulted in low methane production. The most important independent variables impacting COD and sugar solubilization were found to be chemical concentration (as NaOH and HCl), solid content and reaction temperature for the optimization of pretreatment processes. The high amount of methane productions in the range of 222–365 mL CH4 gVS−1 was obtained by the simple thermal application without using chemical agents as NaOH or HCl. Maximum enhancement of methane production was 47–68% compared to raw FVHW when 5% solid content, 1-hour reaction time and 60–100 °C reaction temperature were applied in pretreatments.

ACS Style

Ümmihan Günerhan; Ender Us; Lütfiye Dumlu; Vedat Yılmaz; Hélène Carrère; Altınay.N. Perendeci. Impacts of Chemical-Assisted Thermal Pretreatments on Methane Production from Fruit and Vegetable Harvesting Wastes: Process Optimization. Molecules 2020, 25, 500 .

AMA Style

Ümmihan Günerhan, Ender Us, Lütfiye Dumlu, Vedat Yılmaz, Hélène Carrère, Altınay.N. Perendeci. Impacts of Chemical-Assisted Thermal Pretreatments on Methane Production from Fruit and Vegetable Harvesting Wastes: Process Optimization. Molecules. 2020; 25 (3):500.

Chicago/Turabian Style

Ümmihan Günerhan; Ender Us; Lütfiye Dumlu; Vedat Yılmaz; Hélène Carrère; Altınay.N. Perendeci. 2020. "Impacts of Chemical-Assisted Thermal Pretreatments on Methane Production from Fruit and Vegetable Harvesting Wastes: Process Optimization." Molecules 25, no. 3: 500.

Journal article
Published: 14 December 2019 in Renewable Energy
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Agricultural biogas plants can suffer occasional feedstock shortages (poor harvest, storage…) and recirculation of solid digestate (SD) into digester has been identified as a simple way to offset methane production loss from these situations. Calculations show that recirculation of SD could offset for losses in plant methane production by up to 2.4%. In that context, two post-treatments were evaluated to enhance residual potential methane of agricultural SD. Effect of fungal solid state fermentation (SSF) of SD on subsequent methane production has never been explored before. It was hypothesized that: (i) ligninolytic fungi would be able to specifically use the complex fraction of SD for their growth and (ii) energy generation from the subsequent anaerobic digestion of the colonized SD will be enhanced. However, experiments showed that thermo-alkaline treatment of SD (used as alkalinization and sterilization process) and a high spawn level (20% w/w) were necessary to perform fungal SSF. Besides, the observed fungal activities on SD did not target specifically the most complex fractions. This led to uncontrolled organic matter losses and subsequent decreases of biodegradability and methane yield of SD (up to 50%). Therefore, fungal SSF of SD before its recirculation into biogas plants appeared not to be a viable option.. Only thermo-alkaline treatment (CaO 2% w/w and 121°C 30 min) enhanced methane yield of SD by 13% and decreased its complex fraction by 25%. Further studies on optimization of this post-treatment may enhance efficiency of SD recirculation strategy to offset plant methane production losses.

ACS Style

Ulysse Brémond; Aude Bertrandias; Denis Loisel; Julie Jimenez; Jean-Philippe Steyer; Nicolas Bernet; Hélène Carrere. Assessment of fungal and thermo-alkaline post-treatments of solid digestate in a recirculation scheme to increase flexibility in feedstocks supply management of biogas plants. Renewable Energy 2019, 149, 641 -651.

AMA Style

Ulysse Brémond, Aude Bertrandias, Denis Loisel, Julie Jimenez, Jean-Philippe Steyer, Nicolas Bernet, Hélène Carrere. Assessment of fungal and thermo-alkaline post-treatments of solid digestate in a recirculation scheme to increase flexibility in feedstocks supply management of biogas plants. Renewable Energy. 2019; 149 ():641-651.

Chicago/Turabian Style

Ulysse Brémond; Aude Bertrandias; Denis Loisel; Julie Jimenez; Jean-Philippe Steyer; Nicolas Bernet; Hélène Carrere. 2019. "Assessment of fungal and thermo-alkaline post-treatments of solid digestate in a recirculation scheme to increase flexibility in feedstocks supply management of biogas plants." Renewable Energy 149, no. : 641-651.

Journal article
Published: 11 December 2019 in Bioresource Technology
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This study aims to investigate the effects of microwaves, ultrasonic and alkaline pretreatments on olive pomace properties and its biomethane potential. Alkaline pretreatment was found to reduce lipid and fiber contents (especially lignin) and to increase soluble matter. The alkali pretreatment at a dose of 8% (w/w TS) under 25 °C and for 1 day removed 96% of initial lipids from the solid olive pomace. Unlike NaOH addition, mild microwaves and ultrasonic pretreatments had no impact on lignin. However, in the case of long microwaves pretreatment (450 W-10 min), cellulose and lignin contents were reduced by 50% and 26% respectively. Similarly, the combination of ultrasonic and alkali reagent showed a positive effect on fiber degradation and lipid solubilization as well as a positive impact on methane production. Statistical analysis highlighted the correlation between NaOH dose, solubilization and methane production. The alkaline pretreatment at ambient temperature appeared the most energetically efficient.

ACS Style

Doha Elalami; Helene Carrere; Karima Abdelouahdi; Diana Garcia-Bernet; Jerome Peydecastaing; Guadalupe Vaca-Medina; Abdallah Oukarroum; Youssef Zeroual; Abdellatif Barakat. Mild microwaves, ultrasonic and alkaline pretreatments for improving methane production: Impact on biochemical and structural properties of olive pomace. Bioresource Technology 2019, 299, 122591 .

AMA Style

Doha Elalami, Helene Carrere, Karima Abdelouahdi, Diana Garcia-Bernet, Jerome Peydecastaing, Guadalupe Vaca-Medina, Abdallah Oukarroum, Youssef Zeroual, Abdellatif Barakat. Mild microwaves, ultrasonic and alkaline pretreatments for improving methane production: Impact on biochemical and structural properties of olive pomace. Bioresource Technology. 2019; 299 ():122591.

Chicago/Turabian Style

Doha Elalami; Helene Carrere; Karima Abdelouahdi; Diana Garcia-Bernet; Jerome Peydecastaing; Guadalupe Vaca-Medina; Abdallah Oukarroum; Youssef Zeroual; Abdellatif Barakat. 2019. "Mild microwaves, ultrasonic and alkaline pretreatments for improving methane production: Impact on biochemical and structural properties of olive pomace." Bioresource Technology 299, no. : 122591.

Journal article
Published: 28 October 2019 in Molecules
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The aim of this article is to provide an analysis of microwave effects on ferulic and coumaric acids (FA and CA, respectively) extraction from grass biomass (corn stalks and miscanthus). Microwave pretreatment using various solvents was first compared to conventional heating on corn stalks. Then, microwave operational conditions were extended in terms of incident power and treatment duration. Optimal conditions were chosen to increase p-hydroxycinnamic acids release. Finally, these optimal conditions determined on corn stalks were tested on miscanthus stalks to underlie the substrate incidence on p-hydroxycinnamic acids release yields. The optimal conditions—a treatment duration of 405 s under 1000 W—allowed extracting 1.38% FA and 1.97% CA in corn stalks and 0.58% FA and 3.89% CA in miscanthus stalks. The different bioaccessibility of these two molecules can explain the higher or lower yields between corn and miscanthus stalks.

ACS Style

Aurélie Bichot; Mickaël Lerosty; Laureline Geirnaert; Valérie Méchin; Hélène Carrère; Nicolas Bernet; Jean-Philippe Delgenès; Diana García-Bernet; Bernet; García- Bernet. Soft Microwave Pretreatment to Extract P-Hydroxycinnamic Acids from Grass Stalks. Molecules 2019, 24, 3885 .

AMA Style

Aurélie Bichot, Mickaël Lerosty, Laureline Geirnaert, Valérie Méchin, Hélène Carrère, Nicolas Bernet, Jean-Philippe Delgenès, Diana García-Bernet, Bernet, García- Bernet. Soft Microwave Pretreatment to Extract P-Hydroxycinnamic Acids from Grass Stalks. Molecules. 2019; 24 (21):3885.

Chicago/Turabian Style

Aurélie Bichot; Mickaël Lerosty; Laureline Geirnaert; Valérie Méchin; Hélène Carrère; Nicolas Bernet; Jean-Philippe Delgenès; Diana García-Bernet; Bernet; García- Bernet. 2019. "Soft Microwave Pretreatment to Extract P-Hydroxycinnamic Acids from Grass Stalks." Molecules 24, no. 21: 3885.

Review article
Published: 27 August 2019 in Renewable and Sustainable Energy Reviews
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Currently, sludge is not considered as a waste any more, since it is capable of producing valuable products. Besides land disposal and thermochemical processes (i.e. pyrolysis, combustion, gasification), biological processes appear as promising valorisation routes to treat wastewater sludge efficiently. Anaerobic digestion (AD) processes are already being applied at industrial scales for the effective disposal and valorisation of sludge. However, methane yields from sludge anaerobic digestion remain low compared to other types of organic waste. Thus, pretreatment and co-digestion contribute to improve the degradability of organic matter and methane potential of sludge, respectively. This paper reviews the recent achievements in sludge pretreatment and co-digestion with other substrates such as the organic fraction of municipal solid waste, fatty waste, lignocellulosic and algal biomass. Furthermore, recent studies combining co-digestion and pretreatment are examined. The paper also provides recommendations to better manage sludge recovery by taking into account multiple aspects such as techno-economic feasibility, the effect of pretreatment on both the physico-chemical properties of sludge and the quality of digestate. The socio-environmental and legislative aspects are also essential in order to ensure the sustainability of the process.

ACS Style

D. Elalami; H. Carrere; F. Monlau; K. Abdelouahdi; Abdallah Oukarroum; A. Barakat. Pretreatment and co-digestion of wastewater sludge for biogas production: Recent research advances and trends. Renewable and Sustainable Energy Reviews 2019, 114, 109287 .

AMA Style

D. Elalami, H. Carrere, F. Monlau, K. Abdelouahdi, Abdallah Oukarroum, A. Barakat. Pretreatment and co-digestion of wastewater sludge for biogas production: Recent research advances and trends. Renewable and Sustainable Energy Reviews. 2019; 114 ():109287.

Chicago/Turabian Style

D. Elalami; H. Carrere; F. Monlau; K. Abdelouahdi; Abdallah Oukarroum; A. Barakat. 2019. "Pretreatment and co-digestion of wastewater sludge for biogas production: Recent research advances and trends." Renewable and Sustainable Energy Reviews 114, no. : 109287.

Journal article
Published: 02 April 2019 in Green Chemistry
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Correction for ‘The environmental biorefinery: state-of-the-art on the production of hydrogen and value-added biomolecules in mixed-culture fermentation’ by Roman Moscoviz et al., Green Chem., 2018, 20, 3159–3179.

ACS Style

Roman Moscoviz; Eric Trably; Nicolas Bernet; Hélène Carrère. Correction: The environmental biorefinery: state-of-the-art on the production of hydrogen and value-added biomolecules in mixed-culture fermentation. Green Chemistry 2019, 21, 2148 -2148.

AMA Style

Roman Moscoviz, Eric Trably, Nicolas Bernet, Hélène Carrère. Correction: The environmental biorefinery: state-of-the-art on the production of hydrogen and value-added biomolecules in mixed-culture fermentation. Green Chemistry. 2019; 21 (8):2148-2148.

Chicago/Turabian Style

Roman Moscoviz; Eric Trably; Nicolas Bernet; Hélène Carrère. 2019. "Correction: The environmental biorefinery: state-of-the-art on the production of hydrogen and value-added biomolecules in mixed-culture fermentation." Green Chemistry 21, no. 8: 2148-2148.

Article
Published: 12 February 2019 in BioEnergy Research
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In the context of increasing needs of lignocellulosic biomass for emerging biorefinery, miscanthus is expected to represent a resource for energy production. Regarding biogas production, its potential may be improved either by genotype selection or pretreatment. Eight different miscanthus genotypes belonging to Miscanthus × giganteus (FLO, GID and H8), M. sacchariflorus (GOL, MAL, AUG, H6) and M. sinensis (H5) species were first compared for biomass composition and potential methane. In a second time, alkali pretreatments (NaOH 10 g 100 gTS−1, CaO 10 g 100 gTS−1) were applied at ambient temperature and high solid content, in different conditions of duration and particle size on the genotype FLO presenting the lowest methane potential. The methane potential varied between miscanthus genotypes with values ranging from 166 ± 10 to 202 ± 7 NmLCH4 gVS−1. All of the studied pretreatments increased the methane production up to 55% and reduced Klason lignin and holocellulose contents up to 37%. From this study, NaOH was more efficient than CaO with an increase of the methane production between 24 and 55% and between 19 and 30%, respectively.

ACS Style

Hélène Laurence Thomas; Stéphanie Arnoult; Maryse Brancourt-Hulmel; Hélène Carrère. Methane Production Variability According to Miscanthus Genotype and Alkaline Pretreatments at High Solid Content. BioEnergy Research 2019, 12, 325 -337.

AMA Style

Hélène Laurence Thomas, Stéphanie Arnoult, Maryse Brancourt-Hulmel, Hélène Carrère. Methane Production Variability According to Miscanthus Genotype and Alkaline Pretreatments at High Solid Content. BioEnergy Research. 2019; 12 (2):325-337.

Chicago/Turabian Style

Hélène Laurence Thomas; Stéphanie Arnoult; Maryse Brancourt-Hulmel; Hélène Carrère. 2019. "Methane Production Variability According to Miscanthus Genotype and Alkaline Pretreatments at High Solid Content." BioEnergy Research 12, no. 2: 325-337.

Journal article
Published: 16 January 2019 in Waste Management
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Solid State Anaerobic Digestion (SSAD) of fungal pretreated wheat straw was evaluated in a leach bed reactor. During a first experiment, the effect of Substrate/Inoculum (S/I) ratios on the start-up phase was investigated. High S/I increased methane productivity but also raised the risk of reactor failure due to Volatile Fatty Acid (VFA) accumulation. With S/I ratios between 1.2 and 3.6 (Volatile Solid (VS) basis), the SSAD start-up using wheat straw was successful. Moreover, reactors were able to recover from acidification when the Total VFA/alkalinity ratio was lower than 2 gHAc_eq/gCaCO3, with VFA concentrations lower than 10 g/L and a pH close to 5.5. The conventional threshold of 0.6 gHAc_eq/gCaCO3 for stable wet AD is therefore not adapted to SSAD. During a second experiment, after the wheat straw was submitted to a fungal pretreatment in a non-sterile pilot-scale reactor, it was digested with an S/I ratio of 2.8-2.9. Under batch SSAD conditions, the biodegradability of pretreated wheat straw was slightly improved in comparison to the control (254 versus 215 NmL/g VS, respectively). Considering mass losses occurring during the pretreatment step, suboptimal pretreatment conditions caused a slightly lower methane production (161 versus 171 NmL/gTSinitial after 60-days anaerobic digestion). Nevertheless, pretreatment improved the start-up phase with lower acidification relative to controls. It would be particularly beneficial to improve the methane production in reactors with short reaction times.

ACS Style

Elsa Rouches; Renaud Escudié; Eric Latrille; Hélène Carrère. Solid-state anaerobic digestion of wheat straw: Impact of S/I ratio and pilot-scale fungal pretreatment. Waste Management 2019, 85, 464 -476.

AMA Style

Elsa Rouches, Renaud Escudié, Eric Latrille, Hélène Carrère. Solid-state anaerobic digestion of wheat straw: Impact of S/I ratio and pilot-scale fungal pretreatment. Waste Management. 2019; 85 ():464-476.

Chicago/Turabian Style

Elsa Rouches; Renaud Escudié; Eric Latrille; Hélène Carrère. 2019. "Solid-state anaerobic digestion of wheat straw: Impact of S/I ratio and pilot-scale fungal pretreatment." Waste Management 85, no. : 464-476.

Journal article
Published: 12 December 2018 in Molecules
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This experimental work aims at investigating the effects of milling; sieving; and electrostatic separation on the biochemical methane potential of two olive pomaces from traditional olive oil extraction (M) and from a three-phase system (T). Sieving proved to be efficient for increasing the soluble chemical oxygen demand in the smallest fractions of the sieve of both M (62%) and T (78%) samples. The positive fraction following electrostatic separation also enhanced chemical oxygen demand (COD) solubilisation by 94%, in comparison to sample T milled at 4 mm. Sieve fractions with a size greater than 0.9 mm contained 33% and 47% less lipids for the M and T biomasses; respectively. Dry fractionation modified sample properties as well as lipid and fiber distribution. Concomitantly; milling increased the accessibility and facilitated the release of organic matter. The energy balance was positive after knife milling and sieving; while ball milling and ultrafine milling proved to be inefficient.

ACS Style

Doha ElAlami; Hélène Carrère; Karima Abdelouahdi; Abdallah Oukarroum; Driss Dhiba; Mohamed Arji; Abdellatif Barakat. Combination of Dry Milling and Separation Processes with Anaerobic Digestion of Olive Mill Solid Waste: Methane Production and Energy Efficiency. Molecules 2018, 23, 3295 .

AMA Style

Doha ElAlami, Hélène Carrère, Karima Abdelouahdi, Abdallah Oukarroum, Driss Dhiba, Mohamed Arji, Abdellatif Barakat. Combination of Dry Milling and Separation Processes with Anaerobic Digestion of Olive Mill Solid Waste: Methane Production and Energy Efficiency. Molecules. 2018; 23 (12):3295.

Chicago/Turabian Style

Doha ElAlami; Hélène Carrère; Karima Abdelouahdi; Abdallah Oukarroum; Driss Dhiba; Mohamed Arji; Abdellatif Barakat. 2018. "Combination of Dry Milling and Separation Processes with Anaerobic Digestion of Olive Mill Solid Waste: Methane Production and Energy Efficiency." Molecules 23, no. 12: 3295.

Environmental biotechnology
Published: 07 November 2018 in Applied Microbiology and Biotechnology
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Solid-state fermentation is a potential technology for developing lignocellulosic biomass-based biorefineries. This work dealt with solid-state fermentation for carboxylates production from corn stover, as building blocks for a lignocellulosic feedstock-based biorefinery. The effect of extrusion pretreatment, together with the action of a microbial consortia and hydrolytic enzymes as biotic triggers, was investigated on corn stover conversion, microbial metabolic pathways, and populations. The extrusion caused changes in the physical and morphological characteristics, without altering the biochemical composition of the corn stover. Extrusion also led to remarkable differences in the composition of the indigenous microbial population of the substrate. Consequently, it affected the structure of community developed after fermentation and the substrate conversion yield, which increased by 118% (from 23 ± 4 gCOD/kgVSi obtained with raw substrate to 51 ± 1 gCOD/kgVSi with extruded corn stover) with regard to self-fermentation experiments. The use of activated sludge as inoculum further increased the total substrate conversion into carboxylates, up to 60 ± 2 gCOD/kgVSi, and shaped the microbial communities (mainly composed of bacteria from the Clostridia and Bacteroidia classes) with subsequent homogenization of the fermentation pathways. The addition of hydrolytic enzymes into the reactors further increased the corn stover conversion, leading to a maximum yield of 142 ± 1 gCOD/kgVSi. Thus, extrusion pretreatment combined with the use of an inoculum and enzyme addition increased by 506% corn stover conversion into carboxylates. Beside biomass pretreatment, the results of this study indicated that biotic factor greatly impacted solid-state fermentation by shaping the microbial communities and related metabolic pathways.

ACS Style

Antonella Marone; Eric Trably; Hélène Carrère; Pacome Prompsy; Fabienne Guillon; Maud Joseph-Aimé; Abdellatif Barakat; Nour Fayoud; Nicolas Bernet; Renaud Escudié. Enhancement of corn stover conversion to carboxylates by extrusion and biotic triggers in solid-state fermentation. Applied Microbiology and Biotechnology 2018, 103, 489 -503.

AMA Style

Antonella Marone, Eric Trably, Hélène Carrère, Pacome Prompsy, Fabienne Guillon, Maud Joseph-Aimé, Abdellatif Barakat, Nour Fayoud, Nicolas Bernet, Renaud Escudié. Enhancement of corn stover conversion to carboxylates by extrusion and biotic triggers in solid-state fermentation. Applied Microbiology and Biotechnology. 2018; 103 (1):489-503.

Chicago/Turabian Style

Antonella Marone; Eric Trably; Hélène Carrère; Pacome Prompsy; Fabienne Guillon; Maud Joseph-Aimé; Abdellatif Barakat; Nour Fayoud; Nicolas Bernet; Renaud Escudié. 2018. "Enhancement of corn stover conversion to carboxylates by extrusion and biotic triggers in solid-state fermentation." Applied Microbiology and Biotechnology 103, no. 1: 489-503.

Review paper
Published: 06 October 2018 in Reviews in Environmental Science and Bio/Technology
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One of the main challenges for the deployment of lignocellulosic biorefineries in future years is to find renewable and secured biomass sources in order to obtain bio-sourced products, as an alternative to petroleum-based commodities. Grass biomass, considering its characteristics (availability, composition, productivity, possibility of being harvested from both arable (post-harvest residues) and non-agricultural lands), can be considered as a biomass source for the future. Nevertheless, because of its complex structure and composition, which need deconstructive pre-treatments to render possible further biological conversions, grasses utilisation in biorefinery is today not widespread. Indeed, recalcitrance to polymers degradation in grasses concerns structural and compositional characteristics and can result in costly and complicated biorefinery processes. Grass recalcitrance is due to various natural factors strongly related and difficult to dissociate: rind and vascular structures; composition (lignin content is a key factor for cellulose hydrolysis acting like a physical barrier while hemicelluloses seem to play a more significant role in woody biomass than in grass plants); physical structures (crystalline nature and insoluble surface of cellulose, specific surface area, particle size), etc. Physico-chemical pretreatments are efficient solutions to overcome recalcitrance, while phenotypic selections are interesting but not efficient enough to obtain an optimal enzymatic hydrolysis. In some cases, the structural elements of grass biomass can be negatively affected by physico-chemical pretreatments, causing pre-treatment-induced recalcitrance, like cellulose hornification (irreversible alteration of cellulose microfibers), vascular structure collapsed and reduced cellulose bioaccesibility to enzymes due to cellulose covering by lignin, following lignin solubilisation.

ACS Style

Aurélie Bichot; Jean-Philippe Delgenès; Valérie Mechin; Helene Carrere; Nicolas Bernet; Diana García-Bernet. Understanding biomass recalcitrance in grasses for their efficient utilization as biorefinery feedstock. Reviews in Environmental Science and Bio/Technology 2018, 17, 707 -748.

AMA Style

Aurélie Bichot, Jean-Philippe Delgenès, Valérie Mechin, Helene Carrere, Nicolas Bernet, Diana García-Bernet. Understanding biomass recalcitrance in grasses for their efficient utilization as biorefinery feedstock. Reviews in Environmental Science and Bio/Technology. 2018; 17 (4):707-748.

Chicago/Turabian Style

Aurélie Bichot; Jean-Philippe Delgenès; Valérie Mechin; Helene Carrere; Nicolas Bernet; Diana García-Bernet. 2018. "Understanding biomass recalcitrance in grasses for their efficient utilization as biorefinery feedstock." Reviews in Environmental Science and Bio/Technology 17, no. 4: 707-748.

Original research article
Published: 03 October 2018 in Frontiers in Microbiology
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Microbial consortia producing specific enzymatic cocktails are present in the gut of phytophagous and xylophagous insects; they are known to be the most efficient ecosystems to degrade lignocellulose. Here, the ability of these consortia to degrade ex vivo lignocellulosic biomass in anaerobic bioreactors was characterized in term of bioprocess performances, enzymatic activities and bacterial community structure. In a preliminary screening, guts of Ergates faber (beetle), Potosia cuprea (chafer), Gromphadorrhina portentosa (cockroach), Locusta migratoria (locust), and Gryllus bimaculatus (cricket) were inoculated in anaerobic batch reactors, in presence of grounded wheat straw at neutral pH. A short duration fermentation of less than 8 days was observed and was related to a drop of pH from 7 to below 4.5, leading to an interruption of gas and metabolites production. Consistently, a maximum of 180 mgeq.COD of metabolites accumulated in the medium, which was related to a low degradation of the lignocellulosic biomass, with a maximum of 5 and 2.2% observed for chafer and locust gut consortia. The initial cell-bound and extracellular enzyme activities, i.e., xylanase and β-endoglucanase, were similar to values observed in the literature. Wheat straw fermentation in bioreactors leads to an increase of cell-bounded enzyme activities, with an increase of 145% for cockroach xylanase activity. Bacterial community structures were insect dependent and mainly composed of Clostridia, Bacteroidia and Gammaproteobacteria. Improvement of lignocellulose biodegradation was operated in successive batch mode at pH 8 using the most interesting consortia, i.e., locust, cockroaches and chafer gut consortia. In these conditions, lignocellulose degradation increased significantly: 8.4, 10.5, and 21.0% of the initial COD were degraded for chafer, cockroaches and locusts, respectively in 15 days. Consistently, xylanase activity tripled for the three consortia, attesting the improvement of the process. Bacteroidia was the major bacterial class represented in the bacterial community for all consortia, followed by Clostridia and Gammaproteobacteria classes. This work demonstrates the possibility to maintain apart of insect gut biological activity ex vivo and shows that lignocellulose biodegradation can be improved by using a biomimetic approach. These results bring new insights for the optimization of lignocellulose degradation in bioreactors.

ACS Style

Amandine Gales; Lucile Chatellard; Maider Abadie; Anaïs Bonnafous; Lucas Auer; Helene Carrere; Jean-Jacques Godon; Guillermina Hernandez-Raquet; Claire Dumas. Screening of Phytophagous and Xylophagous Insects Guts Microbiota Abilities to Degrade Lignocellulose in Bioreactor. Frontiers in Microbiology 2018, 9, 2222 .

AMA Style

Amandine Gales, Lucile Chatellard, Maider Abadie, Anaïs Bonnafous, Lucas Auer, Helene Carrere, Jean-Jacques Godon, Guillermina Hernandez-Raquet, Claire Dumas. Screening of Phytophagous and Xylophagous Insects Guts Microbiota Abilities to Degrade Lignocellulose in Bioreactor. Frontiers in Microbiology. 2018; 9 ():2222.

Chicago/Turabian Style

Amandine Gales; Lucile Chatellard; Maider Abadie; Anaïs Bonnafous; Lucas Auer; Helene Carrere; Jean-Jacques Godon; Guillermina Hernandez-Raquet; Claire Dumas. 2018. "Screening of Phytophagous and Xylophagous Insects Guts Microbiota Abilities to Degrade Lignocellulose in Bioreactor." Frontiers in Microbiology 9, no. : 2222.