The outstanding properties of nanocellulose have led to a wide range of applications in packaging, construction, medicine, electronics, cosmetics, environmental solutions, and the food industry. Specifically, cellulose nanocrystals (CNC) have demonstrated excellent biocompatibility, adaptable surface chemistry, low density, optical capabilities, biodegradability, renewability, and good mechanical properties. However, these unique characteristics depend on the raw material, processing, and post-treatment. New opportunities in CNC production are being explored based on unconventional resources and new, environmentally friendly production processes to replace highly polluting and inefficient conventional methods. This review evaluated the current methods for obtaining CNC from green processes, focusing on organic acids, enzymes, mechanical, oxidative, and radiation-based methods.

Bio-polyethylene (BioPE, derived from sugarcane), sugarcane bagasse pulp, and two compatibilizers (fossil and bio-based), were used to manufacture biocomposite filaments for 3D printing. Biocomposite filaments were manufactured and characterized in detail, including measurement of water absorption, mechanical properties, thermal stability and decomposition temperature (thermo-gravimetric analysis (TGA)). Differential scanning calorimetry (DSC) was performed to measure the glass transition temperature (Tg). Scanning electron microscopy (SEM) was applied to assess the fracture area of the filaments after mechanical testing. Increases of up to 10% in water absorption were measured for the samples with 40 wt% fibers and the fossil compatibilizer. The mechanical properties were improved by increasing the fraction of bagasse fibers from 0% to 20% and 40%. The suitability of the biocomposite filaments was tested for 3D printing, and some shapes were printed as demonstrators. Importantly, in a cradle-to-gate life cycle analysis of the biocomposites, we demonstrated that replacing fossil compatibilizer with a bio-based compatibilizer contributes to a reduction in CO₂-eq emissions, and an increase in CO₂ capture, achieving a CO₂-eq storage of 2.12 kg CO₂ eq/kg for the biocomposite containing 40% bagasse fibers and 6% bio-based compatibilizer.

Soda-ethanol pulps, prepared from a forestry residue pine sawdust, were treated according to high-consistency enzymatic fibrillation technology to manufacture nanocellulose. The obtained nanocellulose was characterized and used as ink for three-dimensional (3D) printing of various structures. It was also tested for its moisture sorption capacity and cytotoxicity, as preliminary tests for evaluating its suitability for wound dressing and similar applications. During the high-consistency enzymatic treatment it was found that only the treatment of the O₂-delignified pine pulp resulted in fibrillation into nano-scale. For 3D printing trials, the material needed to be fluidized further. By 3D printing, it was possible to fabricate various structures from the high-consistency enzymatic nanocellulose. However, the water sorption capacity of the structures was lower than previously seen with porous nanocellulose structures, indicating that further optimization of the material is needed. The material was found not to be cytotoxic, thus showing potential as material, e.g., for wound dressings and for printing tissue models.