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Fanrong Zeng
School of Agriculture, Yangtze University, 88 Jingmi Road, Jingzhou 434025, China

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Journal article
Published: 06 April 2021 in Sustainability
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Lead (Pb) is one of the most toxic elements on earth. The main origins of Pb pollution are automobiles, paint and electroplating industries. Pb-induced stress has very toxic effects on plant growth and biomass. The concentration of reactive oxygen species (ROS) in plant cells significantly increases under Pb stress, which interrupts the biochemical cycles in cells and leads to cell death. Therefore, it is essential to clean up the Pb-polluted soils. Among all techniques that are used to clean soil that is metal-contaminated, the best technique is phytoremediation. The present study intends to determine the role of citric acid (CA) and glutathione (GSH) in the phytoremediation of Pb by using castor bean plants. Plant biomass was significantly reduced due to Pb stress. Lead toxicity was also harmful to the photosynthetic pigments and antioxidant enzymes activities. In reverse, the content of malondialdehyde (MDA), H2O2 concentration and electrolyte leakage (EL) were increased under Pb stress. The combined application of GSH and CA enhanced photosynthetic pigments, antioxidant enzyme activities and plant biomass and minimized MDA, H2O2 and EL under Pb stress. The amount of Pb in roots and leaves remarkably increased by the joint application of CA and GSH. The combined application of CA and GSH (5 mM + 25 mM, respectively) was proven to be beneficial compared to the control. From the present results, we can conclude that the combined application of CA and GSH promoted the phytoremediation of Pb and helped the host plant to combat Pb toxicity.

ACS Style

Fanrong Zeng; Zahid Mallhi; Naeem Khan; Muhammad Rizwan; Shafaqat Ali; Awais Ahmad; Afzal Hussain; Abdulaziz Alsahli; Mohammed Alyemeni. Combined Citric Acid and Glutathione Augments Lead (Pb) Stress Tolerance and Phytoremediation of Castorbean through Antioxidant Machinery and Pb Uptake. Sustainability 2021, 13, 4073 .

AMA Style

Fanrong Zeng, Zahid Mallhi, Naeem Khan, Muhammad Rizwan, Shafaqat Ali, Awais Ahmad, Afzal Hussain, Abdulaziz Alsahli, Mohammed Alyemeni. Combined Citric Acid and Glutathione Augments Lead (Pb) Stress Tolerance and Phytoremediation of Castorbean through Antioxidant Machinery and Pb Uptake. Sustainability. 2021; 13 (7):4073.

Chicago/Turabian Style

Fanrong Zeng; Zahid Mallhi; Naeem Khan; Muhammad Rizwan; Shafaqat Ali; Awais Ahmad; Afzal Hussain; Abdulaziz Alsahli; Mohammed Alyemeni. 2021. "Combined Citric Acid and Glutathione Augments Lead (Pb) Stress Tolerance and Phytoremediation of Castorbean through Antioxidant Machinery and Pb Uptake." Sustainability 13, no. 7: 4073.

Journal article
Published: 06 November 2020 in Journal of Hazardous Materials
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Metalloids such as boron and silicon are key elements for plant growth and crop productivity. However, toxic metalloids such as arsenic are increasing in the environment due to inputs from natural sources and human activities. These hazardous metalloids can cause serious health risks to humans and animals if they enter the food chain. Plants have developed highly regulated mechanisms to alleviate the toxicity of metalloids during their 500 million years of evolution. A better understanding the molecular mechanisms underlying the transport and detoxification of toxic metalloids in plants will shed light on developing mitigation strategies. Key transporters and regulatory proteins responsive to toxic metalloids have been identified through evolutionary and molecular analyses. Moreover, knowledge of the regulatory proteins and their pathways can be used in the breeding of crops with lower accumulation of metalloids. These findings can also assist phytoremediation by the exploration of plants such as fern species that hyperaccumulate metalloids from soils and water, and can be used to engineer plants with elevated uptake and storage capacity of toxic metalloids. In summary, there are solutions to remediate contamination due to toxic metalloids by combining the research advances and industrial technologies with agricultural and environmental practices.

ACS Style

Fenglin Deng; Fanrong Zeng; Guang Chen; Xue Feng; Adeel Riaz; Xiaojian Wu; Wei Gao; Feibo Wu; Paul Holford; Zhong-Hua Chen. Metalloid hazards: From plant molecular evolution to mitigation strategies. Journal of Hazardous Materials 2020, 409, 124495 .

AMA Style

Fenglin Deng, Fanrong Zeng, Guang Chen, Xue Feng, Adeel Riaz, Xiaojian Wu, Wei Gao, Feibo Wu, Paul Holford, Zhong-Hua Chen. Metalloid hazards: From plant molecular evolution to mitigation strategies. Journal of Hazardous Materials. 2020; 409 ():124495.

Chicago/Turabian Style

Fenglin Deng; Fanrong Zeng; Guang Chen; Xue Feng; Adeel Riaz; Xiaojian Wu; Wei Gao; Feibo Wu; Paul Holford; Zhong-Hua Chen. 2020. "Metalloid hazards: From plant molecular evolution to mitigation strategies." Journal of Hazardous Materials 409, no. : 124495.

Journal article
Published: 29 October 2020 in International Journal of Molecular Sciences
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Cadmium (Cd) contamination in soils poses great risks to both agricultural production and human health. Calcium (Ca) is an essential element playing a significant role in protecting plants against Cd toxicity. However, how Ca affects Cd uptake and translocation in rice is still not fully elucidated. In this study, the regulatory role of Ca in Cd uptake and upward translocation was investigated in rice at different growth stages. Our results showed that the supplement of 5 mM Ca significantly reduced Cd uptake by rice roots, because of their competition for Ca-permeable channels as an absorption site and Ca-induced downregulation of OsNRAMP1 and OsNRAMP5. However, Ca application facilitated the upward translocation of Cd by both upregulating OsHMA2 to induce xylem loading of Cd and downregulating OsHMA3 to reduce vacuolar sequestration of Cd. Such contrary results suggested a double-edged role of Ca in regulating root Cd uptake and root-to-shoot Cd translocation in rice. Although it increased Cd content in the aboveground vegetative tissues during the whole growth period, the addition of 5 mM Ca eventually decreased Cd content in rice grains at the ripening stage. All these results suggest that Ca-based amendments possess great potential for the production of low-Cd rice grains.

ACS Style

Shuo Zhang; Qi Li; Muhammad Nazir; Shafaqat Ali; Younan Ouyang; Shuzhen Ye; Fanrong Zeng. Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice. International Journal of Molecular Sciences 2020, 21, 8058 .

AMA Style

Shuo Zhang, Qi Li, Muhammad Nazir, Shafaqat Ali, Younan Ouyang, Shuzhen Ye, Fanrong Zeng. Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice. International Journal of Molecular Sciences. 2020; 21 (21):8058.

Chicago/Turabian Style

Shuo Zhang; Qi Li; Muhammad Nazir; Shafaqat Ali; Younan Ouyang; Shuzhen Ye; Fanrong Zeng. 2020. "Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice." International Journal of Molecular Sciences 21, no. 21: 8058.

Journal article
Published: 09 September 2020 in Agronomy
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Chromium (Cr) is recognized as a toxic metal that has detrimental effects on living organisms; notably, it is discharged into soil by various industries as a result of anthropogenic activities. Microbe-assisted phytoremediation is one of the most emergent and environmentally friendly methods used for the detoxification of pollutants. In this study, the alleviative role of Staphylococcus aureus strain K1 was evaluated in wheat (Triticum aestivum L.) under Cr stress. For this, various Cr concentrations (0, 25, 50 and 100 mg·kg−1) with and without peat-moss-based bacterial inoculum were applied in the soil. Results depicted that Cr stress reduced the plants’ growth by causing oxidative stress in the absence of S. aureus K1 inoculation. However, the application of S. aureus K1 regulated the plants’ growth and antioxidant enzymatic activities by reducing oxidative stress and Cr toxicity through conversion of Cr6+ to Cr3+. The Cr6+ uptake by wheat was significantly reduced in the S. aureus K1 inoculated plants. It can be concluded that the application of S. aureus K1 could be an effective approach to alleviate the Cr toxicity in wheat and probably in other cereals grown under Cr stress.

ACS Style

Fanrong Zeng; Munazza Zahoor; Muhammad Waseem; Alia Anayat; Muhammad Rizwan; Awais Ahmad; Tahira Yasmeen; Shafaqat Ali; Mohamed A. El-Sheikh; Mohammed Nasser Alyemeni; Leonard Wijaya. Influence of Metal-Resistant Staphylococcus aureus Strain K1 on the Alleviation of Chromium Stress in Wheat. Agronomy 2020, 10, 1354 .

AMA Style

Fanrong Zeng, Munazza Zahoor, Muhammad Waseem, Alia Anayat, Muhammad Rizwan, Awais Ahmad, Tahira Yasmeen, Shafaqat Ali, Mohamed A. El-Sheikh, Mohammed Nasser Alyemeni, Leonard Wijaya. Influence of Metal-Resistant Staphylococcus aureus Strain K1 on the Alleviation of Chromium Stress in Wheat. Agronomy. 2020; 10 (9):1354.

Chicago/Turabian Style

Fanrong Zeng; Munazza Zahoor; Muhammad Waseem; Alia Anayat; Muhammad Rizwan; Awais Ahmad; Tahira Yasmeen; Shafaqat Ali; Mohamed A. El-Sheikh; Mohammed Nasser Alyemeni; Leonard Wijaya. 2020. "Influence of Metal-Resistant Staphylococcus aureus Strain K1 on the Alleviation of Chromium Stress in Wheat." Agronomy 10, no. 9: 1354.

Original research article
Published: 29 July 2020 in Frontiers in Plant Science
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Drought is a devastating environmental constraint affecting the agronomic production of barley. To facilitate the breeding process, abundant germplasm resources and reliable evaluation systems to identify the true drought-tolerant barley genotypes are needed. In this study, 237 cultivated and 190 wild barley genotypes, originating from 28 countries, were screened for drought tolerance under the conditions of both water deficit and polyethylene glycol (PEG)-simulated drought at seedling stage. Drought stress significantly reduced the plant growth of all barley genotypes, but no significant difference in drought-induced reduction in the performance of barley seedlings was observed under these two drought conditions. Both cultivated and wild barley subspecies displayed considerable genotypic variability in drought tolerance, which underpinned the identification of 18 genotypes contrasting in drought tolerance. A comparative analysis of drought effects on biomass, water relation, photosynthesis, and osmotic adjustment was undertaken using these contrasting barley genotypes, in order to verify the reliability of the screening and to obtain the credible traits as screening criteria of drought tolerance in barley. As expected, the selected drought-tolerant genotypes showed much less reduction in shoot biomass than drought-sensitive ones under water deficit, which was significantly positively correlated with the results of large-scale screening, confirming the reliability of the screening for drought tolerance under two drought conditions in this study. Likewise, the traits of water relation, photosynthetic activity, and osmotic adjustment differed greatly between the contrasting genotypes under water deficit stress, and they were highly correlated to the growth of barley seedlings, suggesting the potential of them to be the selection criteria for drought tolerance. The analysis of the variable importance of these traits in drought tolerance indicated that sap osmolality and relative water content in the youngest fully-expanded leaf are the suitable selection criteria of screening for drought tolerance in barley at seedling stage.

ACS Style

Kangfeng Cai; Xiaohui Chen; Zhigang Han; Xiaojian Wu; Shuo Zhang; Qi Li; Muhammad Mudassir Nazir; Guoping Zhang; Fanrong Zeng. Screening of Worldwide Barley Collection for Drought Tolerance: The Assessment of Various Physiological Measures as the Selection Criteria. Frontiers in Plant Science 2020, 11, 1159 .

AMA Style

Kangfeng Cai, Xiaohui Chen, Zhigang Han, Xiaojian Wu, Shuo Zhang, Qi Li, Muhammad Mudassir Nazir, Guoping Zhang, Fanrong Zeng. Screening of Worldwide Barley Collection for Drought Tolerance: The Assessment of Various Physiological Measures as the Selection Criteria. Frontiers in Plant Science. 2020; 11 ():1159.

Chicago/Turabian Style

Kangfeng Cai; Xiaohui Chen; Zhigang Han; Xiaojian Wu; Shuo Zhang; Qi Li; Muhammad Mudassir Nazir; Guoping Zhang; Fanrong Zeng. 2020. "Screening of Worldwide Barley Collection for Drought Tolerance: The Assessment of Various Physiological Measures as the Selection Criteria." Frontiers in Plant Science 11, no. : 1159.

Journal article
Published: 15 June 2020 in Environmental Pollution
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Understanding of cadmium (Cd) uptake mechanism and development of lower Cd crop genotypes are crucial for combating its phytotoxicity and meeting 70% increase in food demand by 2050. Bio-accumulation of Cd continuously challenges quality of life specifically in regions without adequate environmental planning. Here, we investigated the mechanisms operating in Cd tolerance of two rice genotypes (Heizhan-43 and Yinni-801). Damage to chlorophyll contents and PSII, histochemical staining and quantification of reactive oxygen species (ROS), cell viability and osmolyte accumulation were studied to decipher the interactions between Cd and zinc (Zn) by applying two Cd and two Zn levels (alone as well as combined). Cd2+ and Ca2+ fluxes were also measured by employing sole Cd100 (100 μmol L−1) and Zn50 (50 μmol L−1), and their combination with microelectrode ion flux estimation (MIFE) technique. Cd toxicity substantially reduced chlorophyll contents and maximal photochemical efficiency (Fv/Fm) compared to control plants. Zn supplementation reverted the Cd-induced toxicity by augmenting osmoprotectants and interfering with ROS homeostasis under combined treatments, particularly in Yinni-801 genotype. Fluorescence microscopy indicated a unique pattern of live and dead root cells, depicting more damage with Cd10, Cd15 and Cd15+Zn50. Our results confer that Cd2+ impairs the uptake of Ca2+ whereas, Zn not only competes with Cd2+ but also Ca2+, thereby modifying ion homeostasis in rice plants. This study suggests that exogenous application of Zn is beneficial for rice plants in ameliorating Cd toxicity in a genotype and dose dependent manner by minimizing ROS generation and suppressing collective oxidative damage. The observations confer that Yinni-801 performed better than Heizhan-43 genotype mainly under combined Zn treatments with low-Cd, presenting Zn fortification as a solution to increase rice production.

ACS Style

Muhammad Faheem Adil; Shafaque Sehar; Zhigang Han; Jonas Lwalaba Wa Lwalaba; Ghulam Jilani; Fanrong Zeng; Zhong-Hua Chen; Imran Haider Shamsi. Zinc alleviates cadmium toxicity by modulating photosynthesis, ROS homeostasis, and cation flux kinetics in rice. Environmental Pollution 2020, 265, 114979 .

AMA Style

Muhammad Faheem Adil, Shafaque Sehar, Zhigang Han, Jonas Lwalaba Wa Lwalaba, Ghulam Jilani, Fanrong Zeng, Zhong-Hua Chen, Imran Haider Shamsi. Zinc alleviates cadmium toxicity by modulating photosynthesis, ROS homeostasis, and cation flux kinetics in rice. Environmental Pollution. 2020; 265 ():114979.

Chicago/Turabian Style

Muhammad Faheem Adil; Shafaque Sehar; Zhigang Han; Jonas Lwalaba Wa Lwalaba; Ghulam Jilani; Fanrong Zeng; Zhong-Hua Chen; Imran Haider Shamsi. 2020. "Zinc alleviates cadmium toxicity by modulating photosynthesis, ROS homeostasis, and cation flux kinetics in rice." Environmental Pollution 265, no. : 114979.

Journal article
Published: 18 April 2020 in Food Chemistry
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Phenolic compounds in barley grains have an important influence on beer flavor and stability. Drought condition enhances the content of phenolics in barley grains, leading to reduced malt quality. In this study, two barley genotypes, XZ20 and XZ25 with different total phenolics content were used to investigate the effect of drought on phenolic compounds during grain developing stage. Totally, 118 phenolic metabolites were affected by drought stress. A weighted gene co-expression network analysis (WGCNA) of 17,424 highly expressed genes uncovered black (two hub genes belonged to UGT family) and turquoise modules (three hub genes belonged to phenolics pathway) that are significantly associated with the variation of phenolics. All these results reveal the changes of phenolic metabolites during grain development and provide a new insight into the regulation network of phenolic compounds under drought stress.

ACS Style

Zhigang Han; Muhammad Ahsan; Muhammad Faheem Adil; Xiaohui Chen; Muhammad Mudassir Nazir; Imran Haider Shamsi; Fanrong Zeng; Guoping Zhang. Identification of the gene network modules highly associated with the synthesis of phenolics compounds in barley by transcriptome and metabolome analysis. Food Chemistry 2020, 323, 126862 .

AMA Style

Zhigang Han, Muhammad Ahsan, Muhammad Faheem Adil, Xiaohui Chen, Muhammad Mudassir Nazir, Imran Haider Shamsi, Fanrong Zeng, Guoping Zhang. Identification of the gene network modules highly associated with the synthesis of phenolics compounds in barley by transcriptome and metabolome analysis. Food Chemistry. 2020; 323 ():126862.

Chicago/Turabian Style

Zhigang Han; Muhammad Ahsan; Muhammad Faheem Adil; Xiaohui Chen; Muhammad Mudassir Nazir; Imran Haider Shamsi; Fanrong Zeng; Guoping Zhang. 2020. "Identification of the gene network modules highly associated with the synthesis of phenolics compounds in barley by transcriptome and metabolome analysis." Food Chemistry 323, no. : 126862.

Original article
Published: 20 March 2020 in Plant, Cell & Environment
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This study aimed to reveal the mechanistic basis of the melatonin-mediated amelioration of salinity stress in plants. Electrophysiological experiments revealed that melatonin decreased salt-induced K+ efflux (a critical determinant of plant salt tolerance) in a dose- and time-dependent manner and reduced sensitivity of the plasma membrane K+-permeable channels to hydroxyl radicals. These beneficial effects of melatonin were abolished by NADPH oxidase blocker DPI. Transcriptome analyses revealed that melatonin induced 585 (448 up- and 137 down-regulated) and 59 (54 up- and 5 down-regulated) differentially expressed genes (DEGs) in the root tip and mature zone, respectively. The most noticeable changes in the root tip were melatonin-induced increase in the expression of several DEGs encoding respiratory burst NADPH oxidases (OsRBOHA and OsRBOHF), calcineurin B-like/calcineurin B-like-interacting protein kinase (OsCBL/OsCIPK), and calcium-dependent protein kinase (OsCDPK) under salt stress. Melatonin also enhanced the expression of potassium transporter genes (OsAKT1, OsHAK1, and OsHAK5). Taken together, these results indicate that melatonin improves salt tolerance in rice by enabling K+ retention in roots, and that the latter process is conferred by melatonin scavenging of hydroxyl radicals and a concurrent OsRBOHF-dependent ROS signalling required to activate stress-responsive genes and increase the expression of K+ uptake transporters in the root tip.

ACS Style

Juan Liu; Sergey Shabala; Jing Zhang; Guohui Ma; Dandan Chen; Lana Shabala; Fanrong Zeng; Zhong‐Hua Chen; Meixue Zhou; Gayatri Venkataraman; Quanzhi Zhao. Melatonin improves rice salinity stress tolerance by NADPH oxidase‐dependent control of the plasma membrane K + transporters and K + homeostasis. Plant, Cell & Environment 2020, 43, 2591 -2605.

AMA Style

Juan Liu, Sergey Shabala, Jing Zhang, Guohui Ma, Dandan Chen, Lana Shabala, Fanrong Zeng, Zhong‐Hua Chen, Meixue Zhou, Gayatri Venkataraman, Quanzhi Zhao. Melatonin improves rice salinity stress tolerance by NADPH oxidase‐dependent control of the plasma membrane K + transporters and K + homeostasis. Plant, Cell & Environment. 2020; 43 (11):2591-2605.

Chicago/Turabian Style

Juan Liu; Sergey Shabala; Jing Zhang; Guohui Ma; Dandan Chen; Lana Shabala; Fanrong Zeng; Zhong‐Hua Chen; Meixue Zhou; Gayatri Venkataraman; Quanzhi Zhao. 2020. "Melatonin improves rice salinity stress tolerance by NADPH oxidase‐dependent control of the plasma membrane K + transporters and K + homeostasis." Plant, Cell & Environment 43, no. 11: 2591-2605.

Journal article
Published: 11 March 2020 in Journal of Advances in Modeling Earth Systems
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We document the development and simulation characteristics of the next generation modeling system for seasonal to decadal prediction and projection at the Geophysical Fluid Dynamics Laboratory (GFDL). SPEAR (Seamless System for Prediction and EArth System Research) is built from component models recently developed at GFDL ‐ the AM4 atmosphere model, MOM6 ocean code, LM4 land model and SIS2 sea ice model. The SPEAR models are specifically designed with attributes needed for a prediction model for seasonal to decadal time scales, including the ability to run large ensembles of simulations with available computational resources. For computational speed SPEAR uses a coarse ocean resolution of approximately 1.0o (with tropical refinement). SPEAR can use differing atmospheric horizontal resolutions ranging from 1o to 0.25o. The higher atmospheric resolution facilitates improved simulation of regional climate and extremes. SPEAR is built from the same components as the GFDL CM4 and ESM 4 models, but with design choices geared toward seasonal to multidecadal physical climate prediction and projection. We document simulation characteristics for the time‐mean climate, aspects of internal variability, and the response to both idealized and realistic radiative forcing change. We describe in greater detail one focus of the model development process that was motivated by the importance of the Southern Ocean to the global climate system. We present sensitivity tests that document the influence of the Antarctic surface heat budget on Southern Ocean ventilation and deep global ocean circulation. These findings were also useful in the development processes for the GFDL CM4 and ESM 4 models.

ACS Style

Thomas L. Delworth; William F. Cooke; Alistair Adcroft; Mitchell Bushuk; Jan‐Huey Chen; Krista A. Dunne; Paul Ginoux; Richard Gudgel; Robert W. Hallberg; Lucas Harris; Matthew J. Harrison; Nathaniel Johnson; Sarah B. Kapnick; Shian‐Jian Lin; Feiyu Lu; Sergey Malyshev; Paul C. Milly; Hiroyuki Murakami; Vaishali Naik; Salvatore Pascale; David Paynter; Anthony Rosati; M.D. Schwarzkopf; Elena Shevliakova; Seth Underwood; Andrew T. Wittenberg; BaoQiang Xiang; Xiaosong Yang; Fanrong Zeng; Honghai Zhang; Liping Zhang; Ming Zhao. SPEAR: The Next Generation GFDL Modeling System for Seasonal to Multidecadal Prediction and Projection. Journal of Advances in Modeling Earth Systems 2020, 12, 1 .

AMA Style

Thomas L. Delworth, William F. Cooke, Alistair Adcroft, Mitchell Bushuk, Jan‐Huey Chen, Krista A. Dunne, Paul Ginoux, Richard Gudgel, Robert W. Hallberg, Lucas Harris, Matthew J. Harrison, Nathaniel Johnson, Sarah B. Kapnick, Shian‐Jian Lin, Feiyu Lu, Sergey Malyshev, Paul C. Milly, Hiroyuki Murakami, Vaishali Naik, Salvatore Pascale, David Paynter, Anthony Rosati, M.D. Schwarzkopf, Elena Shevliakova, Seth Underwood, Andrew T. Wittenberg, BaoQiang Xiang, Xiaosong Yang, Fanrong Zeng, Honghai Zhang, Liping Zhang, Ming Zhao. SPEAR: The Next Generation GFDL Modeling System for Seasonal to Multidecadal Prediction and Projection. Journal of Advances in Modeling Earth Systems. 2020; 12 (3):1.

Chicago/Turabian Style

Thomas L. Delworth; William F. Cooke; Alistair Adcroft; Mitchell Bushuk; Jan‐Huey Chen; Krista A. Dunne; Paul Ginoux; Richard Gudgel; Robert W. Hallberg; Lucas Harris; Matthew J. Harrison; Nathaniel Johnson; Sarah B. Kapnick; Shian‐Jian Lin; Feiyu Lu; Sergey Malyshev; Paul C. Milly; Hiroyuki Murakami; Vaishali Naik; Salvatore Pascale; David Paynter; Anthony Rosati; M.D. Schwarzkopf; Elena Shevliakova; Seth Underwood; Andrew T. Wittenberg; BaoQiang Xiang; Xiaosong Yang; Fanrong Zeng; Honghai Zhang; Liping Zhang; Ming Zhao. 2020. "SPEAR: The Next Generation GFDL Modeling System for Seasonal to Multidecadal Prediction and Projection." Journal of Advances in Modeling Earth Systems 12, no. 3: 1.

Research article
Published: 09 January 2020 in Plant Biotechnology Journal
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Plant K+ uptake typically consists low—affinity mechanisms mediated by Shaker K+ channels (AKT/KAT/KC) and high‐affinity mechanisms regulated by HAK/KUP/KT transporters, which are extensively studied. However, the evolutionary and genetic roles of both K+ uptake mechanisms for drought tolerance are not fully explored in crops adapted to dryland agriculture. Here, we employed evolutionary bioinformatics, biotechnological and electrophysiological approaches to determine the role of two important K+ transporters HvAKT2 and HvHAK1 in drought tolerance in barley. HvAKT2 and HvHAK1 were cloned and functionally characterized using barley stripe mosaic virus‐induced gene silencing (BSMV‐VIGS) in drought‐tolerant wild barley XZ5 and agrobacterium‐mediated gene transfer in the barley cultivar Golden Promise. The hallmarks of the K+ selective filters of AKT2 and HAK1 are both found in homologues from strepotophyte algae, and they are evolutionarily conserved in strepotophyte algae and land plants. HvAKT2 and HvHAK1 are both localized to the plasma membrane and have high selectivity to K+ and Rb+ over other tested cations. Overexpression of HvAKT2 and HvHAK1 enhanced K+ uptake and H+ homoeostasis leading to drought tolerance in these transgenic lines. Moreover, HvAKT2‐ and HvHAK1‐overexpressing lines showed distinct response of K+, H+ and Ca2+ fluxes across plasma membrane and production of nitric oxide and hydrogen peroxide in leaves as compared to the wild type and silenced lines. High‐ and low‐affinity K+ uptake mechanisms and their coordination with H+ homoeostasis play essential roles in drought adaptation of wild barley. These findings can potentially facilitate future breeding programs for resilient cereal crops in a changing global climate.

ACS Style

Xue Feng; Wenxing Liu; Cheng‐Wei Qiu; Fanrong Zeng; Yizhou Wang; Guoping Zhang; Zhong‐Hua Chen; Feibo Wu. HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H + homoeostasis. Plant Biotechnology Journal 2020, 18, 1683 -1696.

AMA Style

Xue Feng, Wenxing Liu, Cheng‐Wei Qiu, Fanrong Zeng, Yizhou Wang, Guoping Zhang, Zhong‐Hua Chen, Feibo Wu. HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H + homoeostasis. Plant Biotechnology Journal. 2020; 18 (8):1683-1696.

Chicago/Turabian Style

Xue Feng; Wenxing Liu; Cheng‐Wei Qiu; Fanrong Zeng; Yizhou Wang; Guoping Zhang; Zhong‐Hua Chen; Feibo Wu. 2020. "HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll H + homoeostasis." Plant Biotechnology Journal 18, no. 8: 1683-1696.

Journal article
Published: 06 November 2019 in Plant and Cell Physiology
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Although control of xylem ion loading is essential to confer salinity stress tolerance, specific details behind this process remain elusive. In this work, we compared the kinetics of xylem Na+ and K+ loading between two halophytes (Atriplex lentiformis and quinoa) and two glycophyte (pea and beans) species, to understand the mechanistic basis of the above process. Halophyte plants had high initial amounts of Na+ in the leaf, even when grown in the absence of the salt stress. This was matched by 7-fold higher xylem sap Na+ concentration compared with glycophyte plants. Upon salinity exposure, the xylem sap Na+ concentration increased rapidly but transiently in halophytes, while in glycophytes this increase was much delayed. Electrophysiological experiments using the microelectrode ion flux measuring technique showed that glycophyte plants tend to re-absorb Na+ back into the stele, thus reducing xylem Na+ load at the early stages of salinity exposure. The halophyte plants, however, were capable to release Na+ even in the presence of high Na+ concentrations in the xylem. The presence of hydrogen peroxide (H2O2) [mimicking NaCl stress-induced reactive oxygen species (ROS) accumulation in the root] caused a massive Na+ and Ca2+ uptake into the root stele, while triggering a substantial K+ efflux from the cytosol into apoplast in glycophyte but not halophytes species. The peak in H2O2 production was achieved faster in halophytes (30 min vs 4 h) and was attributed to the increased transcript levels of RbohE. Pharmacological data suggested that non-selective cation channels are unlikely to play a major role in ROS-mediated xylem Na+ loading.

ACS Style

Mahvash Zarei; Sergey Shabala; Fanrong Zeng; Xiaohui Chen; Shuo Zhang; Majid Azizi; Majid Rahemi; Sohrab Davarpanah; Min Yu; Lana Shabala. Comparing Kinetics of Xylem Ion Loading and Its Regulation in Halophytes and Glycophytes. Plant and Cell Physiology 2019, 61, 403 -415.

AMA Style

Mahvash Zarei, Sergey Shabala, Fanrong Zeng, Xiaohui Chen, Shuo Zhang, Majid Azizi, Majid Rahemi, Sohrab Davarpanah, Min Yu, Lana Shabala. Comparing Kinetics of Xylem Ion Loading and Its Regulation in Halophytes and Glycophytes. Plant and Cell Physiology. 2019; 61 (2):403-415.

Chicago/Turabian Style

Mahvash Zarei; Sergey Shabala; Fanrong Zeng; Xiaohui Chen; Shuo Zhang; Majid Azizi; Majid Rahemi; Sohrab Davarpanah; Min Yu; Lana Shabala. 2019. "Comparing Kinetics of Xylem Ion Loading and Its Regulation in Halophytes and Glycophytes." Plant and Cell Physiology 61, no. 2: 403-415.

Original research article
Published: 01 November 2019 in Frontiers in Plant Science
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Rice (Oryza sativa) is a staple food that feeds more than half the world population. As rice is highly sensitive to soil salinity, current trends in soil salinization threaten global food security. To better understand the mechanistic basis of salinity tolerance in rice, three contrasting rice cultivars—Reiziq (tolerant), Doongara (moderately tolerant), and Koshihikari (sensitive)—were examined and the differences in operation of key ion transporters mediating ionic homeostasis in these genotypes were evaluated. Tolerant varieties had reduced Na+ translocation from roots to shoots. Electrophysiological and quantitative reverse transcription PCR experiments showed that tolerant genotypes possessed 2-fold higher net Na+ efflux capacity in the root elongation zone. Interestingly, this efflux was only partially mediated by the plasma membrane Na+/H+ antiporter (OsSOS1), suggesting involvement of some other exclusion mechanisms. No significant difference in Na+ exclusion from the mature root zones was found between cultivars, and the transcriptional changes in the salt overly sensitive signaling pathway genes in the elongation zone were not correlated with the genetic variability in salinity tolerance amongst genotypes. The most important hallmark of differential salinity tolerance was in the ability of the plant to retain K+ in both root zones. This trait was conferred by at least three complementary mechanisms: (1) its superior ability to activate H+-ATPase pump operation, both at transcriptional and functional levels; (2) reduced sensitivity of K+ efflux channels to reactive oxygen species; and (3) smaller upregulation in OsGORK and higher upregulation of OsAKT1 in tolerant cultivars in response to salt stress. These traits should be targeted in breeding programs aimed to improve salinity tolerance in commercial rice cultivars.

ACS Style

Juan Liu; Sergey Shabala; Lana Shabala; Meixue Zhou; Holger Meinke; Gayatri Venkataraman; Zhong-Hua Chen; Fanrong Zeng; Quanzhi Zhao. Tissue-Specific Regulation of Na+ and K+ Transporters Explains Genotypic Differences in Salinity Stress Tolerance in Rice. Frontiers in Plant Science 2019, 10, 1361 .

AMA Style

Juan Liu, Sergey Shabala, Lana Shabala, Meixue Zhou, Holger Meinke, Gayatri Venkataraman, Zhong-Hua Chen, Fanrong Zeng, Quanzhi Zhao. Tissue-Specific Regulation of Na+ and K+ Transporters Explains Genotypic Differences in Salinity Stress Tolerance in Rice. Frontiers in Plant Science. 2019; 10 ():1361.

Chicago/Turabian Style

Juan Liu; Sergey Shabala; Lana Shabala; Meixue Zhou; Holger Meinke; Gayatri Venkataraman; Zhong-Hua Chen; Fanrong Zeng; Quanzhi Zhao. 2019. "Tissue-Specific Regulation of Na+ and K+ Transporters Explains Genotypic Differences in Salinity Stress Tolerance in Rice." Frontiers in Plant Science 10, no. : 1361.

Journal article
Published: 22 August 2019 in International Journal of Molecular Sciences
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In this work, the effect of drought on K+ uptake in root and its translocation from root to shoot was investigated using six barley genotypes contrasting in drought tolerance. Results showed that drought conditions caused significant changes in K+ uptake and translocation in a time- and genotype-specific manner, which consequently resulted in a significant difference in tissue K+ contents and drought tolerance levels between the contrasting barley genotypes. The role of K+ transporters and channels and plasma membrane (PM) H+-ATPase in barley’s adaptive response to drought stress was further investigated at the transcript level. The expression of genes conferring K+ uptake (HvHAK1, HvHAK5, HvKUP1, HvKUP2 and HvAKT1) and xylem loading (HvSKOR) in roots were all affected by drought stress in a time- and genotype-specific manner, indicating that the regulation of these K+ transporters and channels is critical for root K+ uptake and root to shoot K+ translocation in barley under drought stress. Furthermore, the barley genotypes showed a strong correlation between H+ efflux and K+ influx under drought stress, which was further confirmed by the significant up-regulation of HvHA1 and HvHA2. These results suggested an important role of plasma membrane H+-ATPase activity and/or expression in regulating the activity of K+ transporters and channels under drought stress. Taken together, it may be concluded that the genotypic difference in drought stress tolerance in barley is conferred by the difference in the ability to regulate K+ transporters and channels in root epidermis and stele.

ACS Style

Kangfeng Cai; Huaizhou Gao; Xiaojian Wu; Shuo Zhang; Zhigang Han; Xiaohui Chen; Guoping Zhang; Fanrong Zeng. The Ability to Regulate Transmembrane Potassium Transport in Root Is Critical for Drought Tolerance in Barley. International Journal of Molecular Sciences 2019, 20, 4111 .

AMA Style

Kangfeng Cai, Huaizhou Gao, Xiaojian Wu, Shuo Zhang, Zhigang Han, Xiaohui Chen, Guoping Zhang, Fanrong Zeng. The Ability to Regulate Transmembrane Potassium Transport in Root Is Critical for Drought Tolerance in Barley. International Journal of Molecular Sciences. 2019; 20 (17):4111.

Chicago/Turabian Style

Kangfeng Cai; Huaizhou Gao; Xiaojian Wu; Shuo Zhang; Zhigang Han; Xiaohui Chen; Guoping Zhang; Fanrong Zeng. 2019. "The Ability to Regulate Transmembrane Potassium Transport in Root Is Critical for Drought Tolerance in Barley." International Journal of Molecular Sciences 20, no. 17: 4111.

Journal article
Published: 06 February 2019 in International Journal of Molecular Sciences
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Waterlogging is a serious environmental problem that limits agricultural production in low-lying rainfed areas around the world. The major constraint that plants face in a waterlogging situation is the reduced oxygen availability. Accordingly, all previous efforts of plant breeders focused on traits providing adequate supply of oxygen to roots under waterlogging conditions, such as enhanced aerenchyma formation or reduced radial oxygen loss. However, reduced oxygen concentration in waterlogged soils also leads to oxygen deficiency in plant tissues, resulting in an excessive accumulation of reactive oxygen species (ROS) in plants. To the best of our knowledge, this trait has never been targeted in breeding programs and thus represents an untapped resource for improving plant performance in waterlogged soils. To identify the quantitative trait loci (QTL) for ROS tolerance in barley, 187 double haploid (DH) lines from a cross between TX9425 and Naso Nijo were screened for superoxide anion (O2•−) and hydrogen peroxide (H2O2)—two major ROS species accumulated under hypoxia stress. We show that quantifying ROS content after 48 h hypoxia could be a fast and reliable approach for the selection of waterlogging tolerant barley genotypes. The same QTL on chromosome 2H was identified for both O2•− (QSO.TxNn.2H) and H2O2 (QHP.TxNn.2H) contents. This QTL was located at the same position as the QTL for the overall waterlogging and salt tolerance reported in previous studies, explaining 23% and 24% of the phenotypic variation for O2•− and H2O2 contents, respectively. The analysis showed a causal association between ROS production and both waterlogging and salt stress tolerance. Waterlogging and salinity are two major abiotic factors affecting crop production around the globe and frequently occur together. The markers associated with this QTL could potentially be used in future breeding programs to improve waterlogging and salinity tolerance.

ACS Style

Muhammad Bilal Gill; Fanrong Zeng; Lana Shabala; Guoping Zhang; Min Yu; Vadim Demidchik; Sergey Shabala; Meixue Zhou. Identification of QTL Related to ROS Formation under Hypoxia and Their Association with Waterlogging and Salt Tolerance in Barley. International Journal of Molecular Sciences 2019, 20, 699 .

AMA Style

Muhammad Bilal Gill, Fanrong Zeng, Lana Shabala, Guoping Zhang, Min Yu, Vadim Demidchik, Sergey Shabala, Meixue Zhou. Identification of QTL Related to ROS Formation under Hypoxia and Their Association with Waterlogging and Salt Tolerance in Barley. International Journal of Molecular Sciences. 2019; 20 (3):699.

Chicago/Turabian Style

Muhammad Bilal Gill; Fanrong Zeng; Lana Shabala; Guoping Zhang; Min Yu; Vadim Demidchik; Sergey Shabala; Meixue Zhou. 2019. "Identification of QTL Related to ROS Formation under Hypoxia and Their Association with Waterlogging and Salt Tolerance in Barley." International Journal of Molecular Sciences 20, no. 3: 699.

Original paper
Published: 29 October 2018 in Plant Growth Regulation
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Drought is one of the major abiotic stresses affecting crop yields. Understanding drought tolerance mechanism is pivotal for developing drought tolerant crop cultivars. Here, two Tibetan annual wild barley genotypes XZ5 (drought-tolerant) and XZ54 (drought-sensitive) were tested in this study. Gas exchange, stomatal parameters and yield analyses showed that XZ5 has superior drought tolerance than XZ54. Genome-wide transcriptome analysis with epidermal cell layer of XZ5 and XZ54 identified a total of 6,627 genes as drought-induced differentially expressed genes (DEGs) between the two genotypes. The key DEGs could be classified into abscisic acid, brassinosteroid, jasmonic acid, gibberellins, auxin indole-3-acetic acid pathways, reactive oxygen species signaling, Ca2+ signaling, nitric oxide signaling, stomatal development and membrane transport. Moreover, we discovered unique crosstalks among phytohormone pathways, cellular signaling and membrane transport, which are better regulated in the drought tolerant genotype XZ5. For instance, brassinosteroid may participate in co-regulation of stomatal movement with Abscisic acid through suppressing the expression of Brassinosteroid Insensitive 1-Associated Receptor Kinase (HvBAK) to release the interaction target Open Stomata 1 (HvOST1) in barley epidermal layers. This study provides some tissue-specific insights into the role of a crucial layer of cells—leaf epidermis for drought tolerance in the wild progenitors of cultivated barley.

ACS Style

Guang Chen; Yuanyuan Wang; Xiaolei Wang; Qian Yang; Xiaoyan Quan; Jianbin Zeng; Fei Dai; Fanrong Zeng; Feibo Wu; Guoping Zhang; Zhong-Hua Chen. Leaf epidermis transcriptome reveals drought-Induced hormonal signaling for stomatal regulation in wild barley. Plant Growth Regulation 2018, 87, 39 -54.

AMA Style

Guang Chen, Yuanyuan Wang, Xiaolei Wang, Qian Yang, Xiaoyan Quan, Jianbin Zeng, Fei Dai, Fanrong Zeng, Feibo Wu, Guoping Zhang, Zhong-Hua Chen. Leaf epidermis transcriptome reveals drought-Induced hormonal signaling for stomatal regulation in wild barley. Plant Growth Regulation. 2018; 87 (1):39-54.

Chicago/Turabian Style

Guang Chen; Yuanyuan Wang; Xiaolei Wang; Qian Yang; Xiaoyan Quan; Jianbin Zeng; Fei Dai; Fanrong Zeng; Feibo Wu; Guoping Zhang; Zhong-Hua Chen. 2018. "Leaf epidermis transcriptome reveals drought-Induced hormonal signaling for stomatal regulation in wild barley." Plant Growth Regulation 87, no. 1: 39-54.

Journal article
Published: 28 August 2018 in Environmental and Experimental Botany
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ACS Style

Wenxing Liu; Xue Feng; Yue Qiu; Zhong-Hua Chen; Fanrong Zeng; Guoping Zhang; Feibo Wu. Genotypic difference in the influence of aluminum and low pH on ion flux, rhizospheric pH and ATPase activity between Tibetan wild and cultivated barley. Environmental and Experimental Botany 2018, 156, 16 -24.

AMA Style

Wenxing Liu, Xue Feng, Yue Qiu, Zhong-Hua Chen, Fanrong Zeng, Guoping Zhang, Feibo Wu. Genotypic difference in the influence of aluminum and low pH on ion flux, rhizospheric pH and ATPase activity between Tibetan wild and cultivated barley. Environmental and Experimental Botany. 2018; 156 ():16-24.

Chicago/Turabian Style

Wenxing Liu; Xue Feng; Yue Qiu; Zhong-Hua Chen; Fanrong Zeng; Guoping Zhang; Feibo Wu. 2018. "Genotypic difference in the influence of aluminum and low pH on ion flux, rhizospheric pH and ATPase activity between Tibetan wild and cultivated barley." Environmental and Experimental Botany 156, no. : 16-24.

Journal article
Published: 07 August 2018 in Journal of Experimental Botany
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Investigations reveal that Cd can enter into rice cells via Ca-permeable channels, and the uptake varies longitudinally along the rice root.

ACS Style

Xiaohui Chen; Younan Ouyang; Yicong Fan; Boyin Qiu; Guoping Zhang; Fanrong Zeng. The pathway of transmembrane cadmium influx via calcium-permeable channels and its spatial characteristics along rice root. Journal of Experimental Botany 2018, 69, 5279 -5291.

AMA Style

Xiaohui Chen, Younan Ouyang, Yicong Fan, Boyin Qiu, Guoping Zhang, Fanrong Zeng. The pathway of transmembrane cadmium influx via calcium-permeable channels and its spatial characteristics along rice root. Journal of Experimental Botany. 2018; 69 (21):5279-5291.

Chicago/Turabian Style

Xiaohui Chen; Younan Ouyang; Yicong Fan; Boyin Qiu; Guoping Zhang; Fanrong Zeng. 2018. "The pathway of transmembrane cadmium influx via calcium-permeable channels and its spatial characteristics along rice root." Journal of Experimental Botany 69, no. 21: 5279-5291.

Original article
Published: 28 June 2018 in Plant, Cell & Environment
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Efforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii (‘pigface’). We show that storage parenchyma cells of C. rossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+‐ATPase operation and higher non‐enzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of non‐selective cation channels as a major path for Na+ entry. Patch‐clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability was higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels while in mesophyll this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues.

ACS Style

Fanrong Zeng; Sergey Shabala; Jelena Dragišić Maksimović; Vuk Maksimović; Edgar Bonales‐Alatorre; Lana Shabala; Min Yu; Guoping Zhang; Branka Zivanovic. Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi. Plant, Cell & Environment 2018, 41, 2654 -2667.

AMA Style

Fanrong Zeng, Sergey Shabala, Jelena Dragišić Maksimović, Vuk Maksimović, Edgar Bonales‐Alatorre, Lana Shabala, Min Yu, Guoping Zhang, Branka Zivanovic. Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi. Plant, Cell & Environment. 2018; 41 (11):2654-2667.

Chicago/Turabian Style

Fanrong Zeng; Sergey Shabala; Jelena Dragišić Maksimović; Vuk Maksimović; Edgar Bonales‐Alatorre; Lana Shabala; Min Yu; Guoping Zhang; Branka Zivanovic. 2018. "Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi." Plant, Cell & Environment 41, no. 11: 2654-2667.

Journal article
Published: 28 December 2017 in Journal of Experimental Botany
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Oxygen depletion under waterlogged conditions results in a compromised operation of H+-ATPase, with strong implications for membrane potential maintenance, cytosolic pH homeostasis, and transport of all nutrients across membranes. The above effects, however, are highly tissue specific and time dependent, and the causal link between hypoxia-induced changes to the cell's ionome and plant adaptive responses to hypoxia is not well established. This work aimed to fill this gap and investigate the effects of oxygen deprivation on K+ signalling and homeostasis in plants, and potential roles of GORK (depolarization-activated outward-rectifying potassium) channels in adaptation to oxygen-deprived conditions in barley. A significant K+ loss was observed in roots exposed to hypoxic conditions; this loss correlated with the cell's viability. Stress-induced K+ loss was stronger in the root apex immediately after stress onset, but became more pronounced in the root base as the stress progressed. The amount of K+ in shoots of plants grown in waterlogged soil correlated strongly with K+ flux under hypoxia measured in laboratory experiments. Hypoxia induced membrane depolarization; the severity of this depolarization was less pronounced in the tolerant group of cultivars. The expression of GORK was down-regulated by 1.5-fold in mature root but it was up-regulated by 10-fold in the apex after 48 h hypoxia stress. Taken together, our results suggest that the GORK channel plays a central role in K+ retention and signalling under hypoxia stress, and measuring hypoxia-induced K+ fluxes from the mature root zone may be used as a physiological marker to select waterlogging-tolerant varieties in breeding programmes.

ACS Style

Muhammad Bilal Gill; Fanrong Zeng; Lana Shabala; Jennifer Böhm; Guoping Zhang; Meixue Zhou; Sergey Shabala. The ability to regulate voltage-gated K+-permeable channels in the mature root epidermis is essential for waterlogging tolerance in barley. Journal of Experimental Botany 2017, 69, 667 -680.

AMA Style

Muhammad Bilal Gill, Fanrong Zeng, Lana Shabala, Jennifer Böhm, Guoping Zhang, Meixue Zhou, Sergey Shabala. The ability to regulate voltage-gated K+-permeable channels in the mature root epidermis is essential for waterlogging tolerance in barley. Journal of Experimental Botany. 2017; 69 (3):667-680.

Chicago/Turabian Style

Muhammad Bilal Gill; Fanrong Zeng; Lana Shabala; Jennifer Böhm; Guoping Zhang; Meixue Zhou; Sergey Shabala. 2017. "The ability to regulate voltage-gated K+-permeable channels in the mature root epidermis is essential for waterlogging tolerance in barley." Journal of Experimental Botany 69, no. 3: 667-680.

Original research article
Published: 16 November 2017 in Frontiers in Plant Science
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Waterlogging and salinity are two major abiotic stresses that hamper crop production world-wide resulting in multibillion losses. Plant abiotic stress tolerance is conferred by many interrelated mechanisms. Amongst these, the cell’s ability to maintain membrane potential (MP) is considered to be amongst the most crucial traits, a positive relationship between the ability of plants to maintain highly negative MP and its tolerance to both salinity and waterlogging stress. However, no attempts have been made to identify quantitative trait loci (QTL) conferring this trait. In this study, the microelectrode MIFE technique was used to measure the plasma membrane potential of epidermal root cells of 150 double haploid (DH) lines of barley (Hordeum vulgare L.) from a cross between a Chinese landrace TX9425 and Japanese malting cultivar Naso Nijo under hypoxic conditions. A major QTL for the MP in the epidermal root cells in hypoxia-exposed plants was identified. This QTL was located on 2H, at a similar position to the QTL for waterlogging and salinity tolerance reported in previous studies. Further analysis confirmed that MP showed a significant contribution to both waterlogging and salinity tolerance. The fact that the QTL for MP was controlled by a single major QTL illustrates the power of the single-cell phenotyping approach and opens prospects for fine mapping this QTL and thus being more effective in marker assisted selection.

ACS Style

Muhammad B. Gill; Fanrong Zeng; Lana Shabala; Guoping Zhang; Yun Fan; Sergey Shabala; Meixue Zhou. Cell-Based Phenotyping Reveals QTL for Membrane Potential Maintenance Associated with Hypoxia and Salinity Stress Tolerance in Barley. Frontiers in Plant Science 2017, 8, 1 .

AMA Style

Muhammad B. Gill, Fanrong Zeng, Lana Shabala, Guoping Zhang, Yun Fan, Sergey Shabala, Meixue Zhou. Cell-Based Phenotyping Reveals QTL for Membrane Potential Maintenance Associated with Hypoxia and Salinity Stress Tolerance in Barley. Frontiers in Plant Science. 2017; 8 ():1.

Chicago/Turabian Style

Muhammad B. Gill; Fanrong Zeng; Lana Shabala; Guoping Zhang; Yun Fan; Sergey Shabala; Meixue Zhou. 2017. "Cell-Based Phenotyping Reveals QTL for Membrane Potential Maintenance Associated with Hypoxia and Salinity Stress Tolerance in Barley." Frontiers in Plant Science 8, no. : 1.