Two local marine cyanobacteria, Phormidium sp. and Cyanothece sp., were batch-cultured under 18–19.5 °C, at 40 ppt salinity, using white LED light of low (40 μmol photons/m²/s) and high (160 μmol/m²/s) intensity and, additionally, blue, green and red LED light. Yield was highest in high white light in both species (2.15 g dw/L in Phormidium, 1.47 g/L in Cyanothece), followed by green light (1.25 g/L) in Cyanothece and low white and green (1.26–1.33 g/L) in Phormidium. Green light maximized phycocyanin in Phormidium (0.45 mg/mL), while phycoerythrin was enhanced (0.17 mg/mL) by blue light and allophycocyanin by all colors (~0.80 mg/mL). All colors maximized phycocyanin in Cyanothece (~0.32 mg/mL), while phycoerythrin and allophycocyanin peaked under green light (~0.138 and 0.38 mg/mL, respectively). In Phormidium, maximization of chlorophyll-a (9.3 μg/mL) was induced by green light, while total carotenoids and b-carotene (3.05 and 0.89 μg/mL, respectively) by high white light. In Cyanothece, both white light intensities along with green maximized chlorophyll-a (~9 μg/mL) while high white light and green maximized total carotenoids (2.6–3.0 μg/mL). This study strongly indicates that these cyanobacteria can be cultured at the first stage under white light to accumulate sufficient biomass and, subsequently, under colored light for enhancing phycobiliproteins.

A strain of the filamentous non N-fixing cyanobacterium Phormidium sp. isolated from the Messolonghi (W. Greece) saltworks, was cultured in the laboratory at six different combinations of salinity (20-40-60 ppt) and illumination (low-2000 lux and high-8000 lux). At salinities of 60 and 40 ppt and in high illumination (XL-8000 lux), the growth rate (μmax) presented the highest values (0.491 and 0.401, respectively) compared to the corresponding at 20 ppt (0.203). In general and at all salinities, the higher illumination (XL) gave the highest growth rates and shorter duplication time (tg) in comparison to the lower illumination (L). On the contrary, phycocyanin, phycoerythrin and allophycocyanin production was extremely increased in the lower illumination (L) in all salinities, from ~14 fold at 40 and 60 ppt to 269 fold at 20 ppt of those corresponding to higher illumination (XL). Similar analogies were also recorded for the other two billiproteins. Chlorophyll-a content was also higher in lower illumination at all salinities in contrast to total carotenoids that did not exhibit such a pattern. The high growth rate and high phycocyanin content along with the rapid sedimentation of its cultured biomass can set this marine Phormidium species as a promising candidate for mass culture.

Dunaliella salina (Chlorophyceae), Phormidium versicolor (Cyanophyceae), and Cylindrotheca closterium (Bacillariophyceae) were isolated from three ponds in the solar saltern of Sfax (Tunisia). Growth, pigment contents, and photosynthetic and antioxidant enzyme activities were measured under controlled conditions of three light levels (300, 500, and 1000 µmol photons m⁻² s⁻¹) and three NaCl concentrations (40, 80, and 140 g L⁻¹). The highest salinity reduced the growth of D. salina and P. versicolor NCC466 and strongly inhibited that of C. closterium. According to ΦPSII values, the photosynthetic apparatus of P. versicolor was stimulated by increasing salinity, whereas that of D. salina and C. closterium was decreased by irradiance rise. The production of carotenoids in D. salina and P. versicolor was stimulated when salinity and irradiance increased, whereas it decreased in the diatom. Catalase (CAT), Superoxide dismutase (SOD), and Ascorbate peroxidase (APX) activities were only detected when the three species were cultivated under E1000. The antioxidant activity of carotenoids could compensate for the low antioxidant enzyme activity measured in D. salina. Salinity and irradiation levels interact with the physiology of three species that have mechanisms of more or less effective stress resistance, hence different resistance to environmental stresses according to the species. Under these stress-controlled conditions, P. versicolor and C. closterium strains could provide promising sources of extremolyte for several purposes.

After a 1.5-year screening survey in the lagoons of Western Greece in order to isolate and culture sturdy species of microalgae for aquaculture or other value-added uses, as dictated primarily by satisfactory potential for their mass culture, five species emerged, and their growth was monitored in laboratory conditions. Amphidinium carterae, Nephroselmis sp., Tetraselmis sp. (var. red pappas), Asteromonas gracilis, and Dunaliella sp. were batch cultured using low (20 ppt), sea (40 ppt), and high salinity (50 or 60 or 100 ppt) and in combination with low (2000 lux) and high (8000 lux) intensity illumination. The results exhibited that all these species can be grown adequately in all salinities and with the best growth in terms of maximum cell density, specific growth rate (SGR), and biomass yield (g dry weight/L) at high illumination (8000 lux). The five species examined exhibited different responses in the salinities used, whereby Amphidinium clearly performs best in 20 ppt, far better than 40 ppt, and even more so than 50 ppt. Nephroselmis and Tetraselmis grow almost the same in 20 and 40 ppt and less well in 60 ppt. Asteromonas performs best in 100 ppt, although it can grow quite well in both 40 and 60 ppt. Dunaliella grows equally well in all salinities (20, 40, 60 ppt). Concerning the productivity, assessed as the maximum biomass yield at the end of the culture period, the first rank is occupied by Nephroselmis with ~3.0 g d.w./L, followed by Tetraselmis (2.0 g/L), Dunaliella (1.58 g/L), Amphidinium (1.19 g/L), and Asteromonas (0.7 g/L) with all values recorded at high light (8000 lux).

During a survey in 2015, an impressive assemblage of organisms was found in a hypersaline pond of the Messolonghi saltworks. The salinity ranged between 50 and 180 ppt, and the organisms that were found fell into the categories of Cyanobacteria (17 species), Chlorophytes (4 species), Diatoms (23 species), Dinoflagellates (1 species), Protozoa (40 species), Rotifers (8 species), Copepods (1 species), Artemia sp., one nematode and Alternaria sp. (Fungi). Fabrea salina was the most prominent protist among all samples and salinities. This ciliate has the potential to be a live food candidate for marine fish larvae. Asteromonas gracilis proved to be a sturdy microalga, performing well in a broad spectrum of culture salinities. Most of the specimens were identified to the genus level only. Based on their morphology, as there are no relevant records in Greece, there is a possibility for some to be either new species or strikingly different strains of certain species recorded elsewhere.

The ability for food selectivity of rotifer Brachionus plicatiliswas studied in the laboratory by feeding mixtures of microalgae with various cell volumes. Chlorella sp. (≈22 µm3) was the reference a...