The National Park of Dadia in NE Greece (Thrace) was established as a nature reserve in 1980, mainly due to its great diversity in birds of prey. Since then many studies have taken place, focusing on other birds, reptiles, amphibians and some invertebrates (grasshoppers, beetles and butterflies), but up to now none was conducted on spiders. The aim of the present paper was to create the first extensive checklist on the spiders of this important natural reserve. For this purpose, pitfall traps were set in 15 sites located in and around the National Park, resulting in a large spider collection. The results of the taxonomical revision of this collection are here presented, giving rise to 132 species in total, which belong to 24 families. Of them, 11 species (Centromerus valkanovi Deltshev, 1983, Crosbyarachne silvestris (Georgescu, 1973), Ipa terrenus (L. Koch, 1879), Sintula spiniger (Balogh, 1935), Tenuiphantes floriana (van Helsdingen, 1977), Alopecosa taeniopus (Kulczyñski, 1895), Liocranum rupicola (Walckenaer, 1830), Zodarion turcicum Wunderlich, 1980, Gnaphosa modestior Kulczyñski, 1897, Philodromus krausi Muster & Thaler, 2004, Cozyptila thaleri Marusik & Kovblyuk, 2005) are new records for the Greek territory. Seven species (Dysdera kati sp. n., Dysdera krisis sp. n., Harpactea ice sp. n., Harpactea wolfgangi sp. n.—Dysderidae, Phrurolithus thracia sp. n.—Phrurolithidae, Zodarion beroni sp. n.—Zodariidae, Drassyllus dadia sp. n.—Gnaphosidae) are here proposed as new species for science.

New taxonomic data on the ground spiders of the families Gnaphosidae, Liocranidae and Prodidomidae from the Greek islands Rodos and Symi are presented resulting from extensive field work conducted in 2006. Additional material from Karpathos is also included. In total, 48 species are recorded, and five new species (Drassodes mylonasi Chatzaki sp.n., Echemus kaltsasi Chatzaki sp.n., Minosiella apolakia Chatzaki sp.n., Phaeocedus vankeeri Chatzaki sp.n., and Turkozelotes attavirus Chatzaki sp.n.) are proposed. Although the Gnaphosidae of Cyprus are not the focus of the present paper, the unknown female of Echemus levyi Kovblyuk, 2009 found in Cyprus, is also described here for comparative purposes. Leptodrassex memorialis (Spassky, 1940) is justifiably transferred to the genus Leptopilos Levy, 2009 and it is therefore regarded as Leptopilos memorialis comb.nov. The male of Zelotes daidalus Chatzaki, 2003 and the female of Z. minous Chatzaki, 2003 are newly matched and Z. daidalus is further synonymised with Z. shaked Levy, 1998.The new matching of Z. minous males with Z. daidalus females is justified. Two species are new records for Greece: Zelotes limnatis Chatzaki & Russell-Smith, 2017 and Agraecina scupiensis Deltshev, 2016.

New faunistic and taxonomic data are provided for 24 families of Iranian spiders. As a result, six genera (Callilepis, Leptopilos, Parasyrisca (Gnaphosidae), Mastigusa (Hahniidae), Stemonyphantes, and Styloctetor (Linyphiidae)) and 43 species are recorded from Iran for the first time; three species (Hahnia rossiiBrignoli, 1977, Haplodrassus rhodanicus (Simon, 1914), and Mastigusa arietina (Thorell, 1871)) are recorded for the first time from Asia. Additionally, the previously unknown males of Tegenaria erosZamani & Marusik, 2019 and H. rossii are described, and 119 new provincial records are provided for 70 species.

The responses of gnaphosid spiders to an urbanization gradient (urban-suburban-rural areas) were studied using pitfall traps in and near the city of Heraklion, in Crete, Greece, from October 2010 to October 2011. Our results indicate that richness and abundance of species of spiders decreased from the rural area to the center of the city, but not significantly so. Temporal beta diversity was significantly higher in urban areas, which indicates a high temporal variability in species composition of gnaphosid assemblages in the center of the city that takes the form of complementary rather than synchronized phenologies of co-occurring species, as expected in habitats degraded by high levels of disturbance. There was no specific pattern in the body size distribution of the gnaphosids along the urban-rural gradient, and thus the decrease in mean body size with increase in urbanization hypothesis was not supported by our results. However, the species composition recorded in urban areas was very different from that in suburban and rural areas. The percentage of individuals in the catches that were generalist species differed significantly along the gradient. As generalist gnaphosid species made up 72.01% of the total caught in the center of the city, and only 9.53% and 2.07% in suburban and rural areas respectively, our results support the opportunistic species hypothesis. Our analyses indicate that Urozelotes rusticus, a well known synanthropic species recorded for the first time in Greece, is an indicator of high levels of urbanization in Heraklion.