The detection of Ochratoxin A (OTA) in the milk of ruminants occurs infrequently and at low levels, but its occurrence may be higher in dairy products such as cheese. The aim of this study was to investigate the presence of OTA in cheeses purchased in the metropolitan city of Bologna (Italy) and the surrounding area. For the analysis, a LC-MS/MS method with a limit of quantification (LOQ) of 1 µg/kg was used. OTA was detected in seven out of 51 samples of grated hard cheese (concentration range 1.3–22.4 µg/kg), while it was not found in the 33 cheeses of other types which were also analysed. These data show a low risk of OTA contamination for almost all types of cheese analysed. To improve the safety of cheese marketed in grated form, more regulations on cheese rind, which is the part most susceptible to OTA-producing moulds, should be implemented or, alternatively, producers should consider not using the rind as row material for grated cheese. It would be interesting to continue these investigations particularly on grated hard cheeses to have more data to update the risk assessment of OTA in cheese, as also suggested by EFSA in its 2020 scientific opinion on OTA.

The aim of the present study was to determine the levels of ochratoxin A (OTA) in pigs experimentally exposed to this mycotoxin and to evaluate if bile may be used to assess exposure to OTA. Twelve hybrid pigs were divided into 3 equal groups, a control group D₀, and 2 experimental groups, D₁ fed with 50 µg OTA/kg diet, and D₂ fed with 500 µg OTA/kg diet for 15 days. At the end of the test, the animals were euthanized and samples of different tissues and biological fluids were analysed by HPLC-fluorescence detection for the presence of OTA. Samples of unconventional edible tissues such as lung and heart were also taken for analysis because they are used in typical Italian regional dishes. The Italian guidance value for OTA of 1 µg/kg established for pork meat and derived products has been exceeded in all the matrices from both the experimental groups. The comparison between OTA levels detected in D₁ and D₂ groups showed clearly a linear dose-response relationship. Based on the mean values measured, OTA distribution follows the order blood plasma > lung > kidney (in D₁ group), heart (in D₂ group) > heart (in D₁ group), kidney (in D₂ group) > bile > liver > fat > muscle. Analysis of bile can be useful for the detection of OTA in pigs. However, since blood can easily be taken from pigs, and given the correlation between the mycotoxin concentration detected in this matrix and the concentrations detected in the others, OTA level in blood is a more viable approach to assessing the presence of OTA in edible tissues. As lung and heart may contain high concentrations of OTA, the analytical controls should also include these matrices.

The purpose of this study was to provide data on the presence of ochratoxin A (OTA) in artisan salamis produced in the Campania and Marche regions (Italy). For this aim, 96 different salamis were purchased from farms and small salami factories. Analysis were carried out firstly on the casings of all samples, and in a second step, in the outer and inner edible parts of those samples whose casings were found positive for OTA at concentration levels above the Italian guideline value established for OTA in pork meat (1 μg/kg). The analytical method, based on a sample preparation procedure with immunoaffinity columns, together with analysis by LC-MS/MS, has guaranteed average recoveries between 79.4 and 89.0%, limits of detection (LOD) and quantification (LOQ) of 0.10 and 0.25 μg/kg, respectively. OTA was detected in 25.0% of the analysed casings (24 samples) at concentration ranging from 0.25 to 98.52 μg/kg. Ten of these samples were from the same production plant in which an additional sampling was carried out, and where a problem of environmental contamination by ochratoxigenic moulds probably exists. The edible parts were mostly uncontaminated, except in 3 samples, 2 of which showed OTA contamination levels above 1 μg/kg. The presence of OTA on the casing does not seem to be cause for alarm about the safety of this type of product but it should in any case be monitored. Before slicing the meat for consumption, it is always good practice to peel the casing even if reported as edible on salami label.

Pyrrolizidine alkaloids (PAs) are secondary metabolites produced by plants as a self-defense against insects. After bioactivation in the liver, some PAs can cause acute or chronic toxicity in humans. The aim of this study was to determine the presence of PAs in 121 samples of monofloral and multifloral honey from three different Italian regions (Friuli-Venezia Giulia, Marche and Calabria) to meet the European Food Safety Authority (EFSA) suggestion. An in-house liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was validated according to European Union Reference Laboratory (EURL) performance criteria. This method allowed the detection and quantification of 35 PAs. Of the 121 honey samples, 38 (31%), mostly from Calabria, contained PAs. The total content of the PAs ranged from 0.9 µg/kg to 33.1 µg/kg. In particular, echimidine was the most prevalent PA. A rapid human exposure assessment to PAs in honey and a risk characterization was performed using the EFSA RACE tool. The assessment highlighted a potential health concern only for toddlers who frequently consume elevated quantities of honey. This study showed a low presence of PAs in Italian honey; however, the importance of continuously monitoring these compounds is stressed, along with the suggestion that the relevant authorities establish maximum limits to guarantee support for producers and consumer safety.