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Continuous application of phosphate (P) mineral to soil renders apatite addition during each crop growing season which is of great concern from a sustainable agriculture viewpoint. Use of efficient phosphate solubilizing microbes (PSB) is one of the most effective ways to solubilize this apatite mineral in the soil. The current study targeted hydroxyapatite mines to explore, isolate and characterize efficient P solubilizers to solubilize apatite in the soil. Efficiency of isolated microbes to solubilize rock phosphate (hydroxyapatite) and tri-calcium phosphate (TCP) as well as indole-3-acetic acid (IAA) and 1-aminocyclopropane-1-carboxylate deaminase (ACC) activity were tested. Identification and phylogenetic analysis of bacterial and fungal isolates were carried out by 16S rRNA and internal transcribed spacer (ITS) rDNA sequence analyses, respectively. The isolated bacterial strains were identified as Staphylococcus sp., Bacillus firmus, Bacillus safensis, and Bacillus licheniformis whereas fungal isolates were identified as Penicillium sp. and Penicillium oxalicum. Results showed that the impact of identified strains in combination with three phosphate fertilizers sources (compost, rock phosphate and diammonium phosphate (DAP)) was conspicuous on maize crop grown in pot. Both bacterial and fungal strains increased the P uptake by plants as well as recorded with higher available P in post-harvested soil. Penicillium sp. in combination with compost resulted in maximum P-uptake by plants and post-harvest soil P contents, compared to other combinations of P sources and bio-inoculants. Screening and application of efficient P solubilizers can be a better option to utilize the indigenous phosphate reserves of soil as well as organic amendments for sustainable agriculture.
Awais Qarni; Motsim Billah; Khadim Hussain; Sabir Shah; Waqas Ahmed; Sadia Alam; Aftab Sheikh; Laila Jafri; Asia Munir; Kouser Malik; Naeem Khan. Isolation and Characterization of Phosphate Solubilizing Microbes from Rock Phosphate Mines and their Potential Effect for Sustainable Agriculture. Sustainability 2021, 13, 2151 .
AMA StyleAwais Qarni, Motsim Billah, Khadim Hussain, Sabir Shah, Waqas Ahmed, Sadia Alam, Aftab Sheikh, Laila Jafri, Asia Munir, Kouser Malik, Naeem Khan. Isolation and Characterization of Phosphate Solubilizing Microbes from Rock Phosphate Mines and their Potential Effect for Sustainable Agriculture. Sustainability. 2021; 13 (4):2151.
Chicago/Turabian StyleAwais Qarni; Motsim Billah; Khadim Hussain; Sabir Shah; Waqas Ahmed; Sadia Alam; Aftab Sheikh; Laila Jafri; Asia Munir; Kouser Malik; Naeem Khan. 2021. "Isolation and Characterization of Phosphate Solubilizing Microbes from Rock Phosphate Mines and their Potential Effect for Sustainable Agriculture." Sustainability 13, no. 4: 2151.
Salinity is affecting a large area of Pakistan. The country is situated in a semiarid and arid region where the climate is subtropical continental and the process of salinization and sodification is in progress. Excessive salt rates are adversely influencing the biological, chemical, and physical properties of soils. These physical and chemical changes influence the activity of soil microbes and plant roots, and eventually affect the crop growth and yield. Therefore, it is essential from the food security viewpoint to bring these salt-affected lands into agriculture production for the promptly growing population. The paper provides a brief overview of the different aspects of salt-affected soils regarding their extent, origin, existence, nature, types, causes, characteristics, visual diagnosis, effects on soils and plants, salttolerant plant species, management, strategies adopted in Pakistan to overcome salinity, saline, and bio-saline agriculture. Different issues of salinity can be managed by adopting strategies that are helpful in reclamation of such soils. The overview focuses on chemical and biological amendments as they are the most effective implements in improving various soil properties (soil permeability and structural stability) of saline/sodic soils for sustainable food production, thus, authenticating the potential of inorganic and organic amendments to reinstate soil quality. Salinity effects can be reduced to a higher level by the addition of fertilizers and these amendments may also improve nutrient use efficiency. Other sustainable agronomic practices (crop diversifications, leaching by irrigation water, scraping, etc are also pointed out that may inhibit and, in some cases, reverse the conditions of soil salinization.
Aaima Syed; Ghulam Sarwar; Sabir Hussain Shah; Sher Muhammad. Soil Salinity Research in 21st Century in Pakistan: Its Impact on Availability of Plant Nutrients, Growth and Yield of Crops. Communications in Soil Science and Plant Analysis 2020, 52, 183 -200.
AMA StyleAaima Syed, Ghulam Sarwar, Sabir Hussain Shah, Sher Muhammad. Soil Salinity Research in 21st Century in Pakistan: Its Impact on Availability of Plant Nutrients, Growth and Yield of Crops. Communications in Soil Science and Plant Analysis. 2020; 52 (3):183-200.
Chicago/Turabian StyleAaima Syed; Ghulam Sarwar; Sabir Hussain Shah; Sher Muhammad. 2020. "Soil Salinity Research in 21st Century in Pakistan: Its Impact on Availability of Plant Nutrients, Growth and Yield of Crops." Communications in Soil Science and Plant Analysis 52, no. 3: 183-200.
Rock phosphate is a non-renewable resource and a basic raw material of all the water soluble phosphatic fertilizers. The experiment was conducted to estimate usage of different organic amendments on release of P from rock phosphate using maize (Zea mays L.) as a test crop. In the first phase, phosphate rich organic manure (PROM) was prepared using cow dung, press mud and rock phosphate. In the second phase of study, efficiency of thus prepared PROM was evaluated for P release and uptake by maize plants. Treatments of the experiments were; T1 = Recommended NPK, T2 = Recommended NK + zero, T3 = Recommended NK + half Recommended P + Half P from PROM, T4 = Recommended NK = Recommended P + All recommended P from PROM, and T5 = Recommended NK + Recommended P + All recommended P from PROM. The results reveal that the combination of PROM and DAP improved the growth components of maize. PROM also indicated the changes to other soil associated properties like EC (dS m−1) and soil pH. Similarly, PROM and recommended N, K, P from DAP produced the highest value of organic matter (0.94%), soil phosphorus concentration (20.06 mg kg−1), soil potassium concentration (286.2 mg kg−1), nitrogen concentration in plants (3.12%), phosphorous concentration in plants (0.31%), and potassium concentration in plants (3.50%) as compared to other treatments. The results suggest that rock phosphate is an environmental friendly and can be used as an alternate tool to reduce the use of chemical fertilizers.
Kinza Noor; Ghulam Sarwar; Sabir Hussain Shah; Sher Muhammad; Ayesha Zafar; Muhammad Zeeshan Manzoor; Ghulam Murtaza. Formulation of phosphorous rich organic manure from rock phosphate and its dose optimization for the improvement of maize (Zea maysL.). Journal of Plant Nutrition 2020, 44, 96 -119.
AMA StyleKinza Noor, Ghulam Sarwar, Sabir Hussain Shah, Sher Muhammad, Ayesha Zafar, Muhammad Zeeshan Manzoor, Ghulam Murtaza. Formulation of phosphorous rich organic manure from rock phosphate and its dose optimization for the improvement of maize (Zea maysL.). Journal of Plant Nutrition. 2020; 44 (1):96-119.
Chicago/Turabian StyleKinza Noor; Ghulam Sarwar; Sabir Hussain Shah; Sher Muhammad; Ayesha Zafar; Muhammad Zeeshan Manzoor; Ghulam Murtaza. 2020. "Formulation of phosphorous rich organic manure from rock phosphate and its dose optimization for the improvement of maize (Zea maysL.)." Journal of Plant Nutrition 44, no. 1: 96-119.
Plants integrate and monitor low temperature signals to cope with the continual variations in their environment. Arabidopsis thaliana, cold responsive-element binding factor 3 (AtCBF3) plays its role in various cellular activities by modulating multiple genes induced under chilling stress. In this work, AtCBF3 transcription was remarkably induced following chilling stress. AtCBF3-overexpressors namely AtCBF3-Rio Grande, AtCBF3-Moneymaker and AtCBF3-Roma showed defensible response to various levels of chilling stress, while their isogenic wild type plants indicated hypersensitive response to chilling stress. Detailed photosynthetic studies revealed that AtCBF3 gene has harmonious influences on the expression of a large set of genes by virtue of improved stomatal conductance, transpiration rate, intercellular CO2 concentration and photosynthetic rate compared to wild type plants. The AtCBF3-lines limited the water status-mediated hypersensitive response by lowering leaf osmotic potential due to overexpression of AtCBF3 under chilling stress. Biochemical analyses followed by phenotypic studies demonstrated that AtCBF3-plants exhibited membrane stability and lush green appearance by limiting membrane ions leakage and malondialdehyde contents and by accumulating more proline, soluble sugars, chlorophyll contents, carotenoid contents and antioxidant enzymes relative to wild type plants. Hence, with a several lines of evidence, these findings support that tomato transgenic plants overexpressing Arabidopsis CBF3 show enhanced chilling tolerance.
Sabir Hussain Shah; Shaukat Ali; Abdul Ahad Qureshi; Muhammad Amir Zia; Jalal- Ud- Din; Ghulam Muhammad Ali. WITHDRAWN: Physiological and biochemical characterization of tomato transgenic lines overexpressing Arabidopsis thaliana cold responsive-element binding factor 3 (AtCBF3) gene under chilling stress. Journal of Biotechnology 2015, 1 .
AMA StyleSabir Hussain Shah, Shaukat Ali, Abdul Ahad Qureshi, Muhammad Amir Zia, Jalal- Ud- Din, Ghulam Muhammad Ali. WITHDRAWN: Physiological and biochemical characterization of tomato transgenic lines overexpressing Arabidopsis thaliana cold responsive-element binding factor 3 (AtCBF3) gene under chilling stress. Journal of Biotechnology. 2015; ():1.
Chicago/Turabian StyleSabir Hussain Shah; Shaukat Ali; Abdul Ahad Qureshi; Muhammad Amir Zia; Jalal- Ud- Din; Ghulam Muhammad Ali. 2015. "WITHDRAWN: Physiological and biochemical characterization of tomato transgenic lines overexpressing Arabidopsis thaliana cold responsive-element binding factor 3 (AtCBF3) gene under chilling stress." Journal of Biotechnology , no. : 1.