Diverse life forms have evolved internal clocks enabling them to monitor time and thereby anticipate the daily environmental changes caused by Earth's rotation. The plant circadian clock regulates expression of about one-third of the Arabidopsis genome, yet the physiological relevance of this regulation is not fully understood. Here we show that the circadian clock, acting with hormone signals, provides selective advantage to plants through anticipation of and enhanced defense against herbivory. We found that cabbage loopers ( Trichoplusia ni ) display rhythmic feeding behavior that is sustained under constant conditions, and plants entrained in light/dark cycles coincident with the entrainment of the T. ni suffer only moderate tissue loss due to herbivory. In contrast, plants entrained out-of-phase relative to the insects are significantly more susceptible to attack. The in-phase entrainment advantage is lost in plants with arrhythmic clocks or deficient in jasmonate hormone; thus, both the circadian clock and jasmonates are required. Circadian jasmonate accumulation occurs in a phase pattern consistent with preparation for the onset of peak circadian insect feeding behavior, providing evidence for the underlying mechanism of clock-enhanced herbivory resistance. Furthermore, we find that salicylate, a hormone involved in biotrophic defense that often acts antagonistically to jasmonates, accumulates in opposite phase to jasmonates. Our results demonstrate that the plant circadian clock provides a strong physiological advantage by performing a critical role in Arabidopsis defense.

In nature, plants are challenged with hurricane winds, monsoon rains, and herbivory attacks, in addition to many other harsh mechanical perturbations that can threaten plant survival. As a result, over many years of evolution, plants have developed very sensitive mechanisms through which they can perceive and respond to even subtle stimuli, like touch. Some plants respond behaviourally to the touch stimulus within seconds, while others show morphogenetic alterations over long periods of time, ranging from days to weeks. Various signalling molecules and phytohormones, including intracellular calcium, jasmonates, ethylene, abscisic acid, auxin, brassinosteroids, nitric oxide, and reactive oxygen species, have been implicated in touch responses. Many genes are induced following touch. These genes encode proteins involved in various cellular processes including calcium sensing, cell wall modifications, and defence. Twenty-three per cent of these up-regulated genes contain a recently identified promoter element involved in the rapid induction in transcript levels following mechanical perturbations. The employment of various genetic, biochemical, and molecular tools may enable elucidation of the mechanisms through which plants perceive mechano-stimuli and transduce the signals intracellularly to induce appropriate responses.

Soil contamination is an environmental issue due to increasing anthropogenic activities. Existing processes for soil remediation suffer from long treatment time and lack generality because of different sources, occurrences, and properties of pollutants. Here, we report a high-temperature electrothermal process for rapid, water-free remediation of multiple pollutants in soil. The temperature of contaminated soil with carbon additives ramps up to 1000 to 3000 °C as needed within seconds via pulsed direct current input, enabling the vaporization of heavy metals like Cd, Hg, Pb, Co, Ni, and Cu, and graphitization of persistent organic pollutants like polycyclic aromatic hydrocarbons. The rapid treatment retains soil mineral constituents while increases infiltration rate and exchangeable nutrient supply, leading to soil fertilization and improved germination rates. We propose strategies for upscaling and field applications. Techno-economic analysis indicates the process holds the potential for being more energy-efficient and cost-effective compared to soil washing or thermal desorption.