In this study the effect of using a biopolymer soil stabilizer on soil stiffness characteristics was investigated. Chitosan is a bio-waste material that is obtained by chemical treatment of chitin (a chemical component of fungi or crustaceans’ shells). Using chitosan solution as a soil stabilizer is based on the assumption that the biopolymer forms temporary bonds with soil particles. What is important is that these bonds are biodegradable, so the product does not leave any harmful waste and has high eco-compatibility. The biopolymer itself is a by-product of many industrial chemical processes, so its application is compliant with the goals of sustainable geotechnical engineering. The effect of chitosan on soil shear strength, permeability or surface erosion has already been investigated in several different studies. In this study specimens of low-cohesive soil stabilized with two different chitosan solutions were subject to cyclic loading (torsional shearing test) and dynamic loading (resonant column) to obtain soil shear modulus G as a function of strain values. It has been shown that chitosan solution added to medium-grained materials improves their shear modulus G substantially (up to 3 times) even for relatively low chitosan concentration solutions (1.5 g of chitosan per 1 kg of dry silica sand). The results obtained in this study and the known chitosan properties suggest that chitosan solutions can be a very effective and eco-friendly short-term stabilizer for temporary geotechnical structures, e.g., working platforms.