The purpose of this work was to further expand the chemistry of mixed technetium nitrido-peroxo complexes, a still poorly explored class of compounds containing the Tc(VII) moiety, [^99gTc][Tc(N)(O₂)₂]. A number of novel complexes of the formula [^99gTc][Tc(N)(O₂)₂(L)] with bidentate ligands (L) (where L = deprotonated alanine, glycine, proline) were prepared by reacting a solution of nitrido-technetic(VI) acid with L in the presence of a source of H₂O₂. Alternatively, the complex [^99gTc][Tc(N)(O₂)₂X]⁻ (X = Cl, Br) was used as a precursor for substitution reactions where the halogenide ion was replaced by the bidentate ligand. The new complexes were characterized by elemental analysis and mass spectroscopy. The preparation of the analogous [^99mTc][Tc(N)(O₂)₂] moiety, radiolabeled with the metastable isomer Tc-99m, was also studied at a no-carrier-added level, using S-methyl-N-methyl-dithiocarbazate as the donor of the nitrido nitrogen atoms.

Daratumumab is a fully human anti-CD38 monoclonal antibody with strong potential as a targeting vector for therapeutic radionuclides. This study aimed to develop a freeze-dried daratumumab immunoconjugate kit by selecting a suitable chelator (DOTA-NHS, p -SCN-Bn-DOTA, or p -SCN-Bn-1B4M-DTPA) for the ¹⁷⁷ Lu-labelling, optimising the freeze-drying formulation, and evaluating the physicochemical properties and purity profiles. Conjugation performed in carbonate buffer at elevated temperature enhanced chelator incorporation and supported the selection of daratumumab- p -SCN-Bn-DOTA as the most suitable candidate, achieving radiolabelling yield up to 99.8 % without additional purification. Among the evaluated freeze-dried formulations, a saline-based, buffer-free sucrose-mannitol formulation containing polysorbate 20 (S.F5) provided the most favourable characteristics, including minimal residual moisture and the highest monomer purity with non-detectable HMWS species under the applied SE-HPLC conditions. ATR-FTIR and Raman spectroscopy confirmed preservation of the antibody structural integrity after conjugation and freeze-drying. In an in vitro study using human serum, [ ¹⁷⁷ Lu]Lu-daratumumab- p -SCNBn-DOTA maintained higher radiochemical purity for 168 h than [ ¹⁷⁷ Lu]Lu-daratumumab- p -SCN-Bn-1B4MDTPA, indicating greater stability. These results support the feasibility of a ready-to-use freeze-dried daratumumab- p -SCN-Bn-DOTA kit for the ¹⁷⁷ Lu-labelling.

Drying is one of the most critical post-harvest operations in cannabis processing (Nakra et al., 2025; Das et al., 2022) because it directly influences microbiological stability, phytochemical preservation, storage behavior, and downstream extraction performance. Cannabis spp. inflorescences contain high initial moisture levels and a complex mixture of cannabinoids, terpenes, flavonoids, and other bioactive compounds that are highly sensitive to environmental conditions during post-harvest handling. Improper drying may result in microbial contamination, terpene volatilization, cannabinoid degradation, structural damage to glandular trichomes, and reduced extraction efficiency. Environmental parameters including temperature, relative humidity, airflow velocity, and drying duration strongly influence moisture migration kinetics and overall flower quality. This review evaluates the influence of drying technologies and drying conditions on the physicochemical properties of Cannabis sativa L. inflorescences with emphasis on cannabinoid stability, terpene preservation, structural changes of plant tissues, and extraction performance. Particular attention is given to controlled tray drying systems because of their increasing relevance in standardized cannabis processing operations. The review additionally discusses moisture migration mechanisms, drying kinetics, residual moisture behavior, and non-invasive monitoring approaches based on weight reduction during drying. Current challenges and future perspectives for optimization of cannabis drying methodologies are also discussed.

This study presents the results of the stability assessment conducted on three production batches of sodium 18F-fluoride radiopharmaceutical intended for intravenous injection. The objective was to evaluate the physicochemical and microbiological stability, establish an appropriate shelf life, and confirm the overall quality of this radiopharmaceutical over a defined period under controlled storage conditions. The stability study was designed to assess the quality of in-house produced sodium 18F-fluoride radiopharmaceutical, with quality control tests performed at two-hour intervals. All pre-release and post-release tests established in the [18F]NaF specification were examined, including appearance, identification, pH, chemical, radiochemical, radionuclide purity, bacterial endotoxins, and sterility. The results of pH, chemical, radiochemical, and radionuclide purity met the acceptance criteria for all tested points across the three batches, demonstrating the physicochemical stability of the preparation. Microbiological stability was also proved through compliance with bacterial endotoxins and sterility requirements. Overall, the obtained results confirmed that sodium 18F-fluoride is stable for up to 10 hours after the end of synthesis, ensuring its suitability for clinical use.

Background/Objectives: Accurate preoperative localization of hyperfunctioning parathyroid tissue is essential for minimally invasive parathyroidectomy. Conventional planar dual-phase 99mTc-sestamibi scintigraphy is widely used but shows reduced diagnostic accuracy in patients with thyroid nodules or ectopic glands. Hybrid triple-head SPECT/CT integrates functional and anatomical imaging and may improve lesion detection. This study evaluated the diagnostic performance of triple-head SPECT/CT compared with planar scintigraphy and explored correlations between biochemical markers and imaging positivity. Methods: A retrospective single-center study included 90 adults referred for suspected primary hyperparathyroidism between January 2021 and August 2025. Demographic data, laboratory parameters (PTH, total and ionized calcium, 25-hydroxyvitamin D), and imaging results were collected. Diagnostic accuracy was assessed in patients with surgical confirmation or robust clinical verification. Correlations between biochemical markers and imaging positivity were analyzed using Pearson correlation coefficients. Results: SPECT/CT demonstrated significantly higher sensitivity than planar scintigraphy (62.5% vs. 14.3%) and an excellent negative predictive value (95.1%), whereas planar imaging showed slightly higher specificity (79.5%). Ionized calcium correlated significantly with SPECT/CT positivity (r = 0.39; p = 0.009), while PTH and 25-hydroxyvitamin D showed no significant association. SPECT/CT accurately localized ectopic parathyroid glands and lesions in patients with coexisting thyroid nodularity, overcoming limitations of planar imaging. Several lesions undetected by planar scintigraphy were identified on SPECT/CT, supporting its role in anatomically complex or subtle cases. Conclusions: Triple-head SPECT/CT provided superior diagnostic performance over planar scintigraphy for preoperative localization in primary hyperparathyroidism, particularly in patients with thyroid nodularity or ectopic glands. Ionized calcium may serve as a complementary predictor of lesion detectability. These findings support hybrid SPECT/CT as the preferred first-line imaging modality, facilitating targeted minimally invasive surgery and optimizing surgical planning.