Red, black and white seeds quinoa were germinated at 28 °C during 24 (G1), 48 and 72 h (G3). Red quinoa presented a higher percentage of germination with a value of 46% of germination at 72 h. Quinoa protein isolate (QPI) was obtained by alkaline extraction (pH 8.0) followed by an isoelectric precipitation (pH 4.5) from white, red and black quinoa seeds, germinated QPI-G1 or QPI-G3 and non-germinated QPI-NG, Chenopodium quinoa Willd var. Real. QPI-G1, QPI-G3 and QPI-NG were subject to a simulated gastric digestion (DG) and in vitro duodenal digestion (DD). The antioxidant activity was evaluated using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH), azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) methods. Gastric and duodenal digest of QPI-NG and QPI-G1 and QPI-G3 from white, red and black quinoa presented antioxidant activity. QPI-G1-DD of white quinoa presented the highest antioxidant activity with a DPPH value of 167.98 µmoL TE/g of digest, QPI-G1-DD of red quinoa with an ABTS value of 204.86 µmoL TE/g of digest and QPI-G1-DD of black quinoa with an ORAC value of 401.42 µmoL TE/g of digest. QPI-G3-DD of white quinoa presented higher antioxidant activity with a DPPH value of 186.28 µmoL TE/g of sample, QPI-G3-DD of red quinoa with an ABTS value of 144.06 µmoL TE/g of digest and QPI-G3-DD of black quinoa with an ORAC value of 395.14 µmoL TE/g of digest. The inhibition of reactive oxygen species (ROS) production in the zebrafish embryo model (Danio rerio) was evaluated. Protein profiles of QPI from white, red and black from germinated quinoa and non-germinated quinoa were similar with proteins between 10 kDa to 100 kDa with the presence of globulins 11S and 7S and 2S albumins.

This article explores the response of three genotypes of Amaranthus to different sowing date and to different doses of nitrogen (N) fertilization. The experimental design was a randomized complete block design with three replicates for cultivars, planting date and fertilization per treatment. Mexicano sown in early December had the best yield (5285 kg ha^-1) with highly significant differences (p < 0.001) respect to Dorado and Antorcha. To assess the N fertilization Mexicano cultivar was sown at the end of spring (1 December). Morphophysiological variables increased as the N fertilization dose was raised. Higher doses of N fertilizer resulted in greater economic yields this was mainly due to the increase in grain number rather than that of a 1000 grain weight. The results of this investigation suggest that sowing Amaranthus cruentus cv Mexicano on 1 December with 150 kg N ha⁻¹ favors its general development and allows to obtain high economic and biological yields with an appropriate protein content.

Amaranth in southernmost latitudes

Introduction. The production potential of grain amaranth has recently been demonstrated in northeastern Patagonia, Argentina. This crop under irrigation and in a temperate semi-arid climate showed an adequate development of the plants in their different phenological stages with high economic performance. Objectives. This study explored the response of Amaranthus cruentus cv Mexicano to different irrigation frequencies in the lower valley of Río Negro river, Patagonia, Argentina. Method. The experimental design was of three blocks with randomized treatments (subplots), each one corresponding to a different irrigation frequency. The first six irrigations were performed every 7 days for all the treatments, to ensure the establishment of the crop. Then the following treatments were applied: irrigation every 7 days (FI), every 14 days (FII), and every 21 days (FIII). The following biometric variables and their components were measured: height of plant, number of leaves, biomass and economic yield. Results. The results suggest that the optimum irrigation frequency was FII (14 days), resulting in an adequate plant stand at panicle initiation and allows a proper development of plant with optimal biological and economical yields and the highest efficiency of water use (4.02 kg·m-3). Conclusions. The contributions of this study demonstrated the production potential of A. cruentus crop in the lower valley of the Río Negro river under irrigation, representing the southernmost study on irrigation frequency made for this grain crop in the world. A management of irrigation water of 7 days for the establishment of the crop and then with a frequency of 14 days showed the highest yield and the best water use efficiency.

In Argentina, the cultivation of malting barley ( Hordeum vulgare L.) has expanded significantly, driven by the increasing demand of the brewing industry for barley malt. However, the predominance of the Andreia cultivar has reduced genetic diversity, increasing its vulnerability to environmental fluctuations and limiting the selection of new genotypes with desirable malting traits. Based on the hypothesis that significant biochemical variability exists among the commercial genotypes currently available in Argentina, this study provides the first comprehensive assessment integrating germination performance, protein content, hordein composition, and amylolytic enzyme activity in these genotypes. Twelve malting barley genotypes were evaluated under uniform growing conditions, and biochemical analyses were conducted on micromalted grains. Gel Electrophoresis revealed polymorphisms mainly in B‐ and C‐hordeins, supporting genetic differentiation among cultivars. The total protein content ranged from 9.5% to 13.2%, whereas soluble hordeins varied from 5.8% to 12.9% of the total protein. α‐Amylase activity values ≥ 150 U/g were observed in all cultivars evaluated, except for Sinfonía. However, amylolytic activity, which is related to diastatic power, was higher in genotypes such as Andreia, Alhue, Aliciana, and Jennifer. Correlations between protein content, enzyme activity, and hordein profiles indicated genotype‐dependent biochemical patterns in the barley grains. Overall, this study highlights the existence of relevant biochemical diversity among Argentine commercial genotypes, providing valuable information for breeding programs aimed at improving malting quality and promoting productive diversification in the national barley industry.