This page has only limited features, please log in for full access.

Unclaimed
Sabine Müller
Department of Developmental Genetics, Center for Plant Molecular Biology (ZMBP), Tübingen, Germany

Basic Info

Basic Info is private.

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Letter
Published: 24 May 2021 in Nature Plants
Reads 0
Downloads 0

Spatiotemporal control of cell division is essential for the growth and development of multicellular organisms. In plant cells, proper cell plate insertion during cytokinesis relies on the premitotic establishment of the division plane at the cell cortex. Two plant-specific cytoskeleton arrays, the preprophase band (PPB) and the phragmoplast, play important roles in division-plane orientation and cell plate formation, respectively1. Microtubule organization and dynamics and their communication with membranes at the cortex and cell plate are coordinated by multiple, mostly distinct microtubule-associated proteins2. How division-plane selection and establishment are linked, however, is still unknown. Here, we report members of the Arabidopsis IQ67 DOMAIN (IQD) family3 as microtubule-targeted proteins that localize to the PPB and phragmoplast and additionally reside at the cell plate and a polarized cortical region including the cortical division zone (CDZ). IQDs physically interact with PHRAGMOPLAST ORIENTING KINESIN (POK) proteins4,5 and PLECKSTRIN HOMOLOGY GTPase ACTIVATING (PHGAP) proteins6, which are core components of the CDZ1. The loss of IQD function impairs PPB formation and affects CDZ recruitment of POKs and PHGAPs, resulting in division-plane positioning defects. We propose that IQDs act as cellular scaffolds that facilitate PPB formation and CDZ set-up during symmetric cell division. The IQ67 DOMAIN (IQD) family are plant-specific calmodulin-binding proteins. Several members are associated with the microtubule cytoskeleton, and now IQD6, IQD7 and IQD8 are characterized as functioning in the set-up of the cell division plane in the root meristem of Arabidopsis.

ACS Style

Pratibha Kumari; Pradeep Dahiya; Pantelis Livanos; Luise Zergiebel; Malte Kölling; Yvonne Poeschl; Gina Stamm; Arvid Hermann; Steffen Abel; Sabine Müller; Katharina Bürstenbinder. IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation. Nature Plants 2021, 7, 739 -747.

AMA Style

Pratibha Kumari, Pradeep Dahiya, Pantelis Livanos, Luise Zergiebel, Malte Kölling, Yvonne Poeschl, Gina Stamm, Arvid Hermann, Steffen Abel, Sabine Müller, Katharina Bürstenbinder. IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation. Nature Plants. 2021; 7 (6):739-747.

Chicago/Turabian Style

Pratibha Kumari; Pradeep Dahiya; Pantelis Livanos; Luise Zergiebel; Malte Kölling; Yvonne Poeschl; Gina Stamm; Arvid Hermann; Steffen Abel; Sabine Müller; Katharina Bürstenbinder. 2021. "IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation." Nature Plants 7, no. 6: 739-747.

Dataset
Published: 22 April 2021 in Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
Reads 0
Downloads 0

Leaf spongy mesophyll cells form an interconnected network of branched cells and intercellular spaces to maximize the surface area available for light capture and photosynthetic gas exchange. To investigate the morphogenetic events leading to cell separation and branching in Arabidopsis thaliana, we used mesophyll-specific promoters to facilitate imaging of mesophyll cell shape and microtubule (MT) organization over multiple spatiotemporal scales without interference from the overlying epidermal cells. We show that cells enlarge by selective expansion of cell wall regions in contact with intercellular spaces. Cell-cell contacts remain relatively fixed in size, forming the termini of interconnecting branches. Surprisingly, classic schizogeny (de-adhesion of neighboring cells) is relatively infrequent, being related to the local topology of cell junctions during early expansion. Intercellular spaces cue the position of stable MT bundles, which in turn promote efficient dilation of intercellular spaces and cell branching. Our data provide insights into mesophyll morphogenesis and MT organization and lay the groundwork for future investigations.© American Society of Plant Biologists 2020. All rights reserved. For permissions, please email: [email protected]

ACS Style

Sabine Müller; Pantelis Livanos; Zhang L; Mcevoy D; Le Y; Ambrose C. Faculty Opinions recommendation of Live imaging of microtubule organization, cell expansion, and intercellular space formation in Arabidopsis leaf spongy mesophyll cells. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 2021, 33, 1 .

AMA Style

Sabine Müller, Pantelis Livanos, Zhang L, Mcevoy D, Le Y, Ambrose C. Faculty Opinions recommendation of Live imaging of microtubule organization, cell expansion, and intercellular space formation in Arabidopsis leaf spongy mesophyll cells. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. 2021; 33 (3):1.

Chicago/Turabian Style

Sabine Müller; Pantelis Livanos; Zhang L; Mcevoy D; Le Y; Ambrose C. 2021. "Faculty Opinions recommendation of Live imaging of microtubule organization, cell expansion, and intercellular space formation in Arabidopsis leaf spongy mesophyll cells." Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 33, no. 3: 1.

Corrected proof
Published: 17 November 2020 in The Plant Cell
Reads 0
Downloads 0

The bipolar mitotic spindle is a highly conserved structure among eukaryotes that mediates chromosome alignment and segregation. Spindle assembly and size control are facilitated by force-generating microtubule-dependent motor proteins known as kinesins. In animals, kinesin-12 cooperates with kinesin-5 to produce outward-directed forces necessary for spindle assembly. In plants, the relevant molecular mechanisms for spindle formation are poorly defined. While an Arabidopsis thaliana kinesin-5 ortholog has been identified, the kinesin-12 ortholog in plants remains elusive. In this study, we provide experimental evidence for the function of Arabidopsis KINESIN-12E in spindle assembly. In kinesin-12e mutants, a delay in spindle assembly is accompanied by the reduction of spindle size, demonstrating that KINESIN-12E contributes to mitotic spindle architecture. Kinesin-12E localization is mitosis-stage specific, beginning with its perinuclear accumulation during prophase. Upon nuclear envelope breakdown, KINESIN-12E decorates subpopulations of microtubules in the spindle and becomes progressively enriched in the spindle midzone. Furthermore, during cytokinesis, KINESIN-12E shares its localization at the phragmoplast midzone with several functionally diversified Arabidopsis KINESIN-12 members. Changes in the kinetochore and in prophase and metaphase spindle dynamics occur in the absence of KINESIN-12E, suggest it might play an evolutionarily conserved role during spindle formation similar to its spindle-localized animal kinesin-12 orthologs.

ACS Style

Arvid Herrmann; Pantelis Livanos; Steffi Zimmermann; Kenneth Berendzen; Leander Rohr; Elisabeth Lipka; Sabine Müller. KINESIN-12E regulates metaphase spindle flux and helps control spindle size in Arabidopsis. The Plant Cell 2020, 33, 27 -43.

AMA Style

Arvid Herrmann, Pantelis Livanos, Steffi Zimmermann, Kenneth Berendzen, Leander Rohr, Elisabeth Lipka, Sabine Müller. KINESIN-12E regulates metaphase spindle flux and helps control spindle size in Arabidopsis. The Plant Cell. 2020; 33 (1):27-43.

Chicago/Turabian Style

Arvid Herrmann; Pantelis Livanos; Steffi Zimmermann; Kenneth Berendzen; Leander Rohr; Elisabeth Lipka; Sabine Müller. 2020. "KINESIN-12E regulates metaphase spindle flux and helps control spindle size in Arabidopsis." The Plant Cell 33, no. 1: 27-43.

Dataset
Published: 20 August 2020 in Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
Reads 0
Downloads 0

Cell wall assembly requires harmonized deposition of cellulose and matrix polysaccharides. Cortical microtubules orient the deposition of cellulose by guiding the trajectory of cellulose synthase complexes. Vesicles containing matrix polysaccharides are thought to be transported by the FRAGILE FIBER1 (FRA1) kinesin to facilitate their secretion along cortical microtubules. The cortical microtubule cytoskeleton thus may provide a platform to coordinate the delivery of cellulose and matrix polysaccharides, but the underlying molecular mechanisms remain unknown. Here, we show that the tail region of the Arabidopsis (Arabidopsis thaliana) FRA1 kinesin physically interacts with cellulose synthase-microtubule uncoupling (CMU) proteins that are important for the microtubule-dependent guidance of cellulose synthase complexes. Interaction with CMUs did not affect microtubule binding or motility of the FRA1 kinesin but differentially affected the protein levels and microtubule localization of CMU1 and CMU2, thus regulating the lateral stability of cortical microtubules. Phosphorylation of the FRA1 tail region inhibited binding to CMUs and consequently reversed the extent of cortical microtubule decoration by CMU1 and CMU2. Genetic experiments demonstrated the significance of this interaction to the growth and reproduction of Arabidopsis plants. We propose that modulation of CMU protein levels and microtubule localization by FRA1 provides a mechanism that stabilizes the sites of deposition of both cellulose and matrix polysaccharides.© 2020 American Society of Plant Biologists. All rights reserved.

ACS Style

Sabine Müller; Pantelis Livanos; Ganguly A; Zhu C; Chen W; Dixit R. Faculty Opinions recommendation of FRA1 Kinesin Modulates the Lateral Stability of Cortical Microtubules through Cellulose Synthase-Microtubule Uncoupling Proteins. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 2020, 32, 1 .

AMA Style

Sabine Müller, Pantelis Livanos, Ganguly A, Zhu C, Chen W, Dixit R. Faculty Opinions recommendation of FRA1 Kinesin Modulates the Lateral Stability of Cortical Microtubules through Cellulose Synthase-Microtubule Uncoupling Proteins. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. 2020; 32 (8):1.

Chicago/Turabian Style

Sabine Müller; Pantelis Livanos; Ganguly A; Zhu C; Chen W; Dixit R. 2020. "Faculty Opinions recommendation of FRA1 Kinesin Modulates the Lateral Stability of Cortical Microtubules through Cellulose Synthase-Microtubule Uncoupling Proteins." Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 32, no. 8: 1.

Dataset
Published: 12 March 2020 in Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
Reads 0
Downloads 0

The organization of cellulose microfibrils is critical for the strength and growth of plant cell walls. Microtubules have been shown to play a key role in controlling microfibril organization by guiding cellulose synthase complexes [1-4]. However, cellulose synthase trajectories can be maintained when microtubules are removed by drugs, suggesting a separate guidance mechanism is also at play [1, 5, 6]. By slowing down microtubule dynamics, we reveal such a mechanism by showing that cellulose synthase complexes can interact with the trails left by other complexes, causing them to follow the trails or disappear. The stability of the trails, together with the sensitivity of their directions to cellulase treatment, indicates they most likely reflect nascent cellulose microfibrils. Over many hours, this autonomous mechanism alone can lead to a change in the dominant orientation of cellulose synthase trajectories. However, the mechanism can be overridden by the microtubule guidance system. Our findings suggest a dual guidance model, in which an autonomous system, involving interaction between cellulose synthases and microfibrils, can maintain aligned cellulose synthase trajectories, while a microtubule guidance system allows alignments to be steered by environmental and developmental cues.Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.

ACS Style

Sabine Müller; Pantelis Livanos. Faculty Opinions recommendation of Interaction between Autonomous and Microtubule Guidance Systems Controls Cellulose Synthase Trajectories. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 2020, 1 .

AMA Style

Sabine Müller, Pantelis Livanos. Faculty Opinions recommendation of Interaction between Autonomous and Microtubule Guidance Systems Controls Cellulose Synthase Trajectories. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. 2020; ():1.

Chicago/Turabian Style

Sabine Müller; Pantelis Livanos. 2020. "Faculty Opinions recommendation of Interaction between Autonomous and Microtubule Guidance Systems Controls Cellulose Synthase Trajectories." Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature , no. : 1.

Dataset
Published: 08 November 2019 in Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
Reads 0
Downloads 0
ACS Style

Sabine Müller. Faculty Opinions recommendation of Allelic Variation in the Chloroplast Division Gene FtsZ2-2 Leads to Natural Variation in Chloroplast Size. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 2019, 1 .

AMA Style

Sabine Müller. Faculty Opinions recommendation of Allelic Variation in the Chloroplast Division Gene FtsZ2-2 Leads to Natural Variation in Chloroplast Size. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. 2019; ():1.

Chicago/Turabian Style

Sabine Müller. 2019. "Faculty Opinions recommendation of Allelic Variation in the Chloroplast Division Gene FtsZ2-2 Leads to Natural Variation in Chloroplast Size." Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature , no. : 1.

Review article
Published: 19 September 2019 in Current Opinion in Plant Biology
Reads 0
Downloads 0

Many decisions made during plant development depend on the placement of the cytokinetic wall. Cytokinesis involves the biogenesis of the cell plate that progresses centrifugally and until the fusion of the cell plate with the parental cell wall. The phragmoplast facilitates the growth of the cell plate and directs it’s insertion at the cell cortex by a mechanism known as phragmoplast guidance. Communication between the phragmoplast and its destination, the cortical division zone, however, is not well understood. The preprophase band predicts the site of cell plate fusion, seemingly controlling the site of the cortical division zone establishment, but recent results suggest the role of this cytoskeletal array to be rather subtle. This is indirectly supported by certain types of phragmoplast-driven cell division in mosses and algae, which lack preprophase bands. In this review article, we summarize recent insight concerning phragmoplast expansion and guidance.

ACS Style

Henrik Buschmann; Sabine Müller. Update on plant cytokinesis: rule and divide. Current Opinion in Plant Biology 2019, 52, 97 -105.

AMA Style

Henrik Buschmann, Sabine Müller. Update on plant cytokinesis: rule and divide. Current Opinion in Plant Biology. 2019; 52 ():97-105.

Chicago/Turabian Style

Henrik Buschmann; Sabine Müller. 2019. "Update on plant cytokinesis: rule and divide." Current Opinion in Plant Biology 52, no. : 97-105.

Review
Published: 28 August 2019 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Kinesin-12 family members are characterized by an N-terminal motor domain and the extensive presence of coiled-coil domains. Animal orthologs display microtubule plus-end directed motility, bundling of parallel and antiparallel microtubules, plus-end stabilization, and they play a crucial role in spindle assembly. In plants, kinesin-12 members mediate a number of developmental processes including male gametophyte, embryo, seedling, and seed development. At the cellular level, they participate in critical events during cell division. Several kinesin-12 members localize to the phragmoplast midzone, interact with isoforms of the conserved microtubule cross-linker MICROTUBULE-ASSOCIATED PROTEIN 65 (MAP65) family, and are required for phragmoplast stability and expansion, as well as for proper cell plate development. Throughout cell division, a subset of kinesin-12 reside, in addition or exclusively, at the cortical division zone and mediate the accurate guidance of the phragmoplast. This review aims to summarize the current knowledge on kinesin-12 in plants and shed some light onto the heterogeneous localization and domain architecture, which potentially conceals functional diversification.

ACS Style

Sabine Müller; Pantelis Livanos. Plant Kinesin-12: Localization Heterogeneity and Functional Implications. International Journal of Molecular Sciences 2019, 20, 4213 .

AMA Style

Sabine Müller, Pantelis Livanos. Plant Kinesin-12: Localization Heterogeneity and Functional Implications. International Journal of Molecular Sciences. 2019; 20 (17):4213.

Chicago/Turabian Style

Sabine Müller; Pantelis Livanos. 2019. "Plant Kinesin-12: Localization Heterogeneity and Functional Implications." International Journal of Molecular Sciences 20, no. 17: 4213.

Dataset
Published: 22 May 2019 in Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
Reads 0
Downloads 0

Root hairs are tubular protrusions of the root epidermis that significantly enlarge the exploitable soil volume in the rhizosphere. Trichoblasts, the cell type responsible for root hair formation, switch from cell elongation to tip growth through polarization of the growth machinery to a predefined root hair initiation domain (RHID) at the plasma membrane. The emergence of this polar domain resembles the establishment of cell polarity in other eukaryotic systems [1-3]. Rho-type GTPases of plants (ROPs) are among the first molecular determinants of the RHID [4, 5], and later play a central role in polar growth [6]. Numerous studies have elucidated mechanisms that position the RHID in the cell [7-9] or regulate ROP activity [10-18]. The molecular players that target ROPs to the RHID and initiate outgrowth, however, have not been identified. We dissected the timing of the growth machinery assembly in polarizing hair cells and found that positioning of molecular players and outgrowth are temporally separate processes that are each controlled by specific ROP guanine nucleotide exchange factors (GEFs). A functional analysis of trichoblast-specific GEFs revealed GEF3 to be required for normal ROP polarization and thus efficient root hair emergence, whereas GEF4 predominantly regulates subsequent tip growth. Ectopic expression of GEF3 induced the formation of spatially confined, ROP-recruiting domains in other cell types, demonstrating the role of GEF3 to serve as a membrane landmark during cell polarization.Copyright © 2019 Elsevier Ltd. All rights reserved.

ACS Style

Sabine Müller; Denninger P; Reichelt A; Schmidt Vaf; Mehlhorn Dg; Asseck Ly; Stanley Ce; Keinath Nf; Evers Jf; Grefen C; Grossmann G. Faculty Opinions recommendation of Distinct ropgefs successively drive polarization and outgrowth of root hairs. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 2019, 1 .

AMA Style

Sabine Müller, Denninger P, Reichelt A, Schmidt Vaf, Mehlhorn Dg, Asseck Ly, Stanley Ce, Keinath Nf, Evers Jf, Grefen C, Grossmann G. Faculty Opinions recommendation of Distinct ropgefs successively drive polarization and outgrowth of root hairs. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. 2019; ():1.

Chicago/Turabian Style

Sabine Müller; Denninger P; Reichelt A; Schmidt Vaf; Mehlhorn Dg; Asseck Ly; Stanley Ce; Keinath Nf; Evers Jf; Grefen C; Grossmann G. 2019. "Faculty Opinions recommendation of Distinct ropgefs successively drive polarization and outgrowth of root hairs." Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature , no. : 1.

Journal article
Published: 29 April 2019 in Annual Review of Plant Biology
Reads 0
Downloads 0

Plant cells divide their cytoplasmic content by forming a new membrane compartment, the cell plate, via a rerouting of the secretory pathway toward the division plane aided by a dynamic cytoskeletal apparatus known as the phragmoplast. The phragmoplast expands centrifugally and directs the cell plate to the preselected division site at the plasma membrane to fuse with the parental wall. The division site is transiently decorated by the cytoskeletal preprophase band in preprophase and prophase, whereas a number of proteins discovered over the last decade reside continuously at the division site and provide a lasting spatial reference for phragmoplast guidance. Recent studies of membrane fusion at the cell plate have revealed the contribution of functionally conserved eukaryotic proteins to distinct stages of cell plate biogenesis and emphasize the coupling of cell plate formation with phragmoplast expansion. Together with novel findings concerning preprophase band function and the setup of the division site, cytokinesis and its spatial control remain an open-ended field with outstanding and challenging questions to resolve.

ACS Style

Pantelis Livanos; Sabine Müller. Division Plane Establishment and Cytokinesis. Annual Review of Plant Biology 2019, 70, 239 -267.

AMA Style

Pantelis Livanos, Sabine Müller. Division Plane Establishment and Cytokinesis. Annual Review of Plant Biology. 2019; 70 (1):239-267.

Chicago/Turabian Style

Pantelis Livanos; Sabine Müller. 2019. "Division Plane Establishment and Cytokinesis." Annual Review of Plant Biology 70, no. 1: 239-267.

Review article
Published: 15 April 2019 in Current Opinion in Cell Biology
Reads 0
Downloads 0

The plant microtubules form unique arrays using acentrosomal microtubule nucleation pathways, yet utilizing evolutionary conserved centrosomal proteins. In cytokinesis, a multi-component cytoskeletal apparatus, the phragmoplast mediates the biosynthesis of the new cell plate by dynamic centrifugal expansion, a process that demands exquisite coordination of microtubule turnover and endomembrane trafficking. At the same time, the phragmoplast is guided to meet with the parental wall at a cortical site that is predefined before mitotic entry and transiently marked by the preprophase band of microtubules. The cortical division zone maintains positional information of the selected division plane for the entire duration of cell division and for the guidance of the phragmoplast during cytokinesis. Its establishment is an essential requirement for normal plant organogenesis, due to the confinement of cells by rigid cell walls.

ACS Style

Sabine Müller. Plant cell division — defining and finding the sweet spot for cell plate insertion. Current Opinion in Cell Biology 2019, 60, 9 -18.

AMA Style

Sabine Müller. Plant cell division — defining and finding the sweet spot for cell plate insertion. Current Opinion in Cell Biology. 2019; 60 ():9-18.

Chicago/Turabian Style

Sabine Müller. 2019. "Plant cell division — defining and finding the sweet spot for cell plate insertion." Current Opinion in Cell Biology 60, no. : 9-18.

Conference paper
Published: 21 February 2019 in Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
Reads 0
Downloads 0

In most flowering plants, the asymmetric cell division of the zygote is the initial step in establishing the apical-basal axis of the mature plant. The zygote is polarized, possessing the nucleus at the apical tip and large vacuoles at the basal end. Despite their known polar localization, whether the positioning of the vacuoles and the nucleus is coordinated and what the role of the vacuole is in the asymmetric zygotic division remain elusive. In the present study, we utilized a live-cell imaging system to visualize the dynamics of vacuoles during the entire process of zygote polarization in Arabidopsis Image analysis revealed that the vacuoles formed tubular strands around the apically migrating nucleus. They gradually accumulated at the basal region and filled the space, resulting in asymmetric distribution in the mature zygote. To assess the role of vacuoles in the zygote, we screened various vacuole mutants and identified that shoot gravitropism2 (sgr2), in which the vacuolar structural change was impaired, failed to form tubular vacuoles and to polarly distribute the vacuole. In sgr2, large vacuoles occupied the apical tip and thus nuclear migration was blocked, resulting in a more symmetric zygotic division. We further observed that tubular vacuole formation and asymmetric vacuolar distribution both depended on the longitudinal array of actin filaments. Overall, our results show that vacuolar dynamics is crucial not only for the polar distribution along actin filaments but also for adequate nuclear positioning, and consequently zygote-division asymmetry.

ACS Style

Sabine Müller; Kimata Y; Kato T; Higaki T; Kurihara D; Yamada T; Segami S; Morita Mt; Maeshima M; Hasezawa S; Higashiyama T; Tasaka M; Ueda M. Faculty Opinions recommendation of Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 2019, 116, 1 .

AMA Style

Sabine Müller, Kimata Y, Kato T, Higaki T, Kurihara D, Yamada T, Segami S, Morita Mt, Maeshima M, Hasezawa S, Higashiyama T, Tasaka M, Ueda M. Faculty Opinions recommendation of Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. 2019; 116 (6):1.

Chicago/Turabian Style

Sabine Müller; Kimata Y; Kato T; Higaki T; Kurihara D; Yamada T; Segami S; Morita Mt; Maeshima M; Hasezawa S; Higashiyama T; Tasaka M; Ueda M. 2019. "Faculty Opinions recommendation of Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes." Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 116, no. 6: 1.

Articles
Published: 13 December 2018 in Entrepreneurship & Regional Development
Reads 0
Downloads 0

Balancing social and economic missions in the pursuit of growth is one of the greatest challenges faced by social ventures. Although social ventures strive for growth to scale their social impact, pursuing growth often results in mission drift and the sacrifice of social objectives, which in turn eventually undermine the ventures’ raison d’être. In this study, we investigate how and with what outcomes social ventures that pursue growth can manage the balance of social and economic missions. Through a comparative case study of six for-profit social ventures, we find significant differences in how dual missions are selected, connected, and intertwined, leading to varying degrees of mission spillover effects between social and economic missions. Our findings show that two-sided mission spillover effects are a central mechanism in dual mission management, enabling social ventures to pursue balanced growth, avoid mission drift, and achieve social impact. With these findings, this study adds to the emergent literature on social entrepreneurship, dual mission management, and social venture growth.

ACS Style

Nicole Siebold; Franziska Günzel-Jensen; Sabine Müller. Balancing dual missions for social venture growth: a comparative case study. Entrepreneurship & Regional Development 2018, 31, 710 -734.

AMA Style

Nicole Siebold, Franziska Günzel-Jensen, Sabine Müller. Balancing dual missions for social venture growth: a comparative case study. Entrepreneurship & Regional Development. 2018; 31 (9-10):710-734.

Chicago/Turabian Style

Nicole Siebold; Franziska Günzel-Jensen; Sabine Müller. 2018. "Balancing dual missions for social venture growth: a comparative case study." Entrepreneurship & Regional Development 31, no. 9-10: 710-734.

Original article
Published: 20 November 2018 in Journal of Mental Health
Reads 0
Downloads 0

Background: Many adolescents with mental illness (MI) struggle with the decision whether to disclose their condition. They may decide to keep their MI secret, whether due to fear of public stigma or due to self-stigma and shame. Secrecy may protect against discrimination, but has often negative long-term consequences such as social isolation. Aim: To explore personal views of adolescents with MI on secrecy and disclosure of their MI. Method: Six focus groups consisting of 39 adolescents with MI were recorded, transcribed, and analyzed by qualitative content analysis to identify major themes deductively and inductively. Results: Participants described MI as a stigmatized condition and stressed both the benefits and risks of secrecy. Disadvantages included fear of stigma and loss of friendships, benefits included emotional support and relief. Adolescents underlined the importance of individual disclosure decisions (DDs). The majority preferred selective disclosure. Additionally, the role of other adolescents with and without MI was emphasized. Conclusions: DDs are individual and depend on social factors since stigma and fear of discrimination remain significant concerns for adolescents with MI. Implications for future interventions to support adolescents with MI are discussed.

ACS Style

Nadine Mulfinger; Nicolas Rüsch; Philipp Bayha; Sabine Müller; Isabel Böge; Vehbi Sakar; Silvia Krumm. Secrecy versus disclosure of mental illness among adolescents: I. The perspective of adolescents with mental illness. Journal of Mental Health 2018, 28, 296 -303.

AMA Style

Nadine Mulfinger, Nicolas Rüsch, Philipp Bayha, Sabine Müller, Isabel Böge, Vehbi Sakar, Silvia Krumm. Secrecy versus disclosure of mental illness among adolescents: I. The perspective of adolescents with mental illness. Journal of Mental Health. 2018; 28 (3):296-303.

Chicago/Turabian Style

Nadine Mulfinger; Nicolas Rüsch; Philipp Bayha; Sabine Müller; Isabel Böge; Vehbi Sakar; Silvia Krumm. 2018. "Secrecy versus disclosure of mental illness among adolescents: I. The perspective of adolescents with mental illness." Journal of Mental Health 28, no. 3: 296-303.

Dataset
Published: 19 November 2018 in Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
Reads 0
Downloads 0

Cell wall appositions (CWAs) are produced reactively by the plant immune system to arrest microbial invasion through the local inversion of plant cell growth. This process requires the controlled invagination of the plasma membrane (PM) in coordination with the export of barrier material to the volume between the plant PM and cell wall. Plant actin dynamics are essential to this response, but it remains unclear how exocytosis and the cytoskeleton are linked in space and time to form functional CWAs. Here, we show that actin-dependent trafficking to immune response sites of Arabidopsis thaliana delivers membrane-integrated FORMIN4, which in turn contributes to local cytoskeletal dynamics. Total internal reflection fluorescence (TIRF) microscopy combined with controlled induction of FORMIN4-GFP expression reveals a dynamic population of vesicular bodies that accumulate to form clusters at the PM through an actin-dependent process. Deactivation of FORMIN4 and its close homologs partially compromises subsequent defense and alters filamentous actin (F-actin) distribution at mature CWAs. The localization of FORMIN4 is stable and segregated from the dynamic traffic of the endosomal network. Moreover, the tessellation of FORMIN4 at the PM with meso-domains of PEN3 reveals a fine spatial segregation of destinations for actin-dependent immunity cargo. Together, our data suggest a model where FORMIN4 is a spatial feedback element in a multi-layered, temporally defined sequence of cytoskeletal response. This positional feedback makes a significant contribution to the distribution of actin filaments at the dynamic CWA boundary and to the outcomes of pre-invasion defense.Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

ACS Style

Sabine Müller; Sassmann S; Rodrigues C; Milne Sw; Nenninger A; Allwood E; Littlejohn Gr; Talbot Nj; Soeller C; Davies B; Hussey Pj; Deeks Mj. Faculty Opinions recommendation of An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 2018, 28, 1 .

AMA Style

Sabine Müller, Sassmann S, Rodrigues C, Milne Sw, Nenninger A, Allwood E, Littlejohn Gr, Talbot Nj, Soeller C, Davies B, Hussey Pj, Deeks Mj. Faculty Opinions recommendation of An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. 2018; 28 (13):1.

Chicago/Turabian Style

Sabine Müller; Sassmann S; Rodrigues C; Milne Sw; Nenninger A; Allwood E; Littlejohn Gr; Talbot Nj; Soeller C; Davies B; Hussey Pj; Deeks Mj. 2018. "Faculty Opinions recommendation of An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants." Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 28, no. 13: 1.

Journal article
Published: 12 July 2018 in EMBO reports
Reads 0
Downloads 0

Kinesins are versatile nano‐machines that utilize variable non‐motor domains to tune specific motor microtubule encounters. During plant cytokinesis, the kinesin‐12 orthologs, PHRAGMOPLAST ORIENTING KINESIN (POK)1 and POK2, are essential for rapid centrifugal expansion of the cytokinetic apparatus, the phragmoplast, toward a pre‐selected cell plate fusion site at the cell cortex. Here, we report on the spatio‐temporal localization pattern of POK2, mediated by distinct protein domains. Functional dissection of POK2 domains revealed the association of POK2 with the site of the future cell division plane and with the phragmoplast during cytokinesis. Accumulation of POK2 at the phragmoplast midzone depends on its functional POK2 motor domain and is fine‐tuned by its carboxy‐terminal region that also directs POK2 to the division site. Furthermore, POK2 likely stabilizes the phragmoplast midzone via interaction with the conserved microtubule‐associated protein MAP65‐3/PLEIADE, a well‐established microtubule cross‐linker. Collectively, our results suggest that dual localized POK2 plays multiple roles during plant cell division.

ACS Style

Arvid Herrmann; Pantelis Livanos; Elisabeth Lipka; Astrid Gadeyne; Marie‐Theres Hauser; Daniël Van Damme; Sabine Müller. Dual localized kinesin‐12 POK 2 plays multiple roles during cell division and interacts with MAP 65‐3. EMBO reports 2018, 19, e46085 .

AMA Style

Arvid Herrmann, Pantelis Livanos, Elisabeth Lipka, Astrid Gadeyne, Marie‐Theres Hauser, Daniël Van Damme, Sabine Müller. Dual localized kinesin‐12 POK 2 plays multiple roles during cell division and interacts with MAP 65‐3. EMBO reports. 2018; 19 (9):e46085.

Chicago/Turabian Style

Arvid Herrmann; Pantelis Livanos; Elisabeth Lipka; Astrid Gadeyne; Marie‐Theres Hauser; Daniël Van Damme; Sabine Müller. 2018. "Dual localized kinesin‐12 POK 2 plays multiple roles during cell division and interacts with MAP 65‐3." EMBO reports 19, no. 9: e46085.

Journal article
Published: 01 July 2018 in Biophysical Journal
Reads 0
Downloads 0

Plant development and morphology relies on the accurate insertion of new cell walls during cytokinesis. However, how a plant cell correctly orients a new wall is poorly understood. Two kinesin class-12 members, phragmoplast orienting kinesin 1 (POK1) and POK2, are involved in the process, but how these molecular machines work is not known. Here, we used in vivo and single-molecule in vitro measurements to determine how Arabidopsis thaliana POK2 motors function mechanically. We found that POK2 is a very weak, on average plus-end-directed, moderately fast kinesin. Interestingly, POK2 switches between processive and diffusive modes characterized by an exclusive-state mean-squared-displacement analysis. Our results support a model that POK motors push against peripheral microtubules of the phragmoplast for its guidance. This pushing model may mechanically explain the conspicuous narrowing of the division site. Together, our findings provide mechanical insight into how active motors accurately position new cell walls in plants.

ACS Style

Mayank Chugh; Maja Reißner; Michael Bugiel; Elisabeth Lipka; Arvid Herrmann; Basudev Roy; Sabine Mueller; Erik Schäffer. Phragmoplast Orienting Kinesin 2 Is a Weak Motor Switching between Processive and Diffusive Modes. Biophysical Journal 2018, 115, 375 -385.

AMA Style

Mayank Chugh, Maja Reißner, Michael Bugiel, Elisabeth Lipka, Arvid Herrmann, Basudev Roy, Sabine Mueller, Erik Schäffer. Phragmoplast Orienting Kinesin 2 Is a Weak Motor Switching between Processive and Diffusive Modes. Biophysical Journal. 2018; 115 (2):375-385.

Chicago/Turabian Style

Mayank Chugh; Maja Reißner; Michael Bugiel; Elisabeth Lipka; Arvid Herrmann; Basudev Roy; Sabine Mueller; Erik Schäffer. 2018. "Phragmoplast Orienting Kinesin 2 Is a Weak Motor Switching between Processive and Diffusive Modes." Biophysical Journal 115, no. 2: 375-385.

Dataset
Published: 04 June 2018 in Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
Reads 0
Downloads 0

Microtubule dependent motor proteins dynein and kinesin accomplish diverse intracellular functions, contributing to intracellular transport and cytoskeletal organization. Cytoplasmic dynein is a highly processive motor protein facilitating long distance transport by walking long distances towards microtubule minus ends before detaching. Although most kinesins move towards microtubule plus-ends, the kinesin-14 class comprises mainly...

ACS Style

Sabine Muller; Tseng Kf; Wang P; Lee Yj; Bowen J; Gicking Am; Guo L; Liu B; Qiu W. Faculty Opinions recommendation of The preprophase band-associated kinesin-14 OsKCH2 is a processive minus-end-directed microtubule motor. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 2018, 9, 1 .

AMA Style

Sabine Muller, Tseng Kf, Wang P, Lee Yj, Bowen J, Gicking Am, Guo L, Liu B, Qiu W. Faculty Opinions recommendation of The preprophase band-associated kinesin-14 OsKCH2 is a processive minus-end-directed microtubule motor. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. 2018; 9 (1):1.

Chicago/Turabian Style

Sabine Muller; Tseng Kf; Wang P; Lee Yj; Bowen J; Gicking Am; Guo L; Liu B; Qiu W. 2018. "Faculty Opinions recommendation of The preprophase band-associated kinesin-14 OsKCH2 is a processive minus-end-directed microtubule motor." Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 9, no. 1: 1.

Dataset
Published: 14 March 2018 in Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
Reads 0
Downloads 0

In this study, the authors report on the interaction between the nuclear transport receptor importin β (IMB) and the kinesin-4 motor protein FRA1. While importins function to shuttle proteins into the nucleus, the kinesin-4 FRA1 is involved in rapid cell growth. Importins usually sequester their targets in the nucleus until their activity is required, as demonstrated for metazoan kinesins. However, FRA1...

ACS Style

Sabine Muller; Ganguly A; DeMott L; Zhu C; Mcclosky Dd; Anderson Ct; Dixit R. Faculty Opinions recommendation of Importin-β Directly Regulates the Motor Activity and Turnover of a Kinesin-4. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 2018, 44, 1 .

AMA Style

Sabine Muller, Ganguly A, DeMott L, Zhu C, Mcclosky Dd, Anderson Ct, Dixit R. Faculty Opinions recommendation of Importin-β Directly Regulates the Motor Activity and Turnover of a Kinesin-4. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. 2018; 44 (5):1.

Chicago/Turabian Style

Sabine Muller; Ganguly A; DeMott L; Zhu C; Mcclosky Dd; Anderson Ct; Dixit R. 2018. "Faculty Opinions recommendation of Importin-β Directly Regulates the Motor Activity and Turnover of a Kinesin-4." Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 44, no. 5: 1.

Randomized controlled trial
Published: 05 December 2017 in Journal of Child Psychology and Psychiatry
Reads 0
Downloads 0

Due to public stigma or self-stigma and shame, many adolescents with mental illness (MI) struggle with the decision whether to disclose their MI to others. Both disclosure and nondisclosure are associated with risks and benefits. Honest, Open, Proud (HOP) is a peer-led group program that supports participants with disclosure decisions in order to reduce stigma's impact. Previously, HOP had only been evaluated among adults with MI. This two-arm pilot randomized controlled trial included 98 adolescents with MI. Participants were randomly assigned to HOP and treatment as usual (TAU) or to TAU alone. Outcomes were assessed pre (T0/baseline), post (T1/after the HOP program), and at 3-week follow-up (T2/6 weeks after T0). Primary endpoints were stigma stress at T1 and quality of life at T2. Secondary outcomes included self-stigma, disclosure-related distress, empowerment, help-seeking intentions, recovery, and depressive symptoms. The trial is registered on ClinicalTrials (NCT02751229; http://www.clinicaltrials.gov). Compared to TAU, adolescents in the HOP program showed significantly reduced stigma stress at T1 (d = .92, p < .001) and increased quality of life at T2 (d = .60, p = .004). In a longitudinal mediation model, the latter effect was fully mediated by stigma stress reduction at T1. HOP further showed significant positive effects on self-stigma, disclosure-related distress, secrecy, help-seeking intentions, attitudes to disclosure, recovery, and depressive symptoms. Effects at T1 remained stable or improved further at follow-up. In a limited economic evaluation HOP was cost-efficient in relation to gains in quality of life. As HOP is a compact three-session program and showed positive effects on stigma and disclosure variables as well as on symptoms and quality of life, it could help to reduce stigma's negative impact among adolescents with MI.

ACS Style

Nadine Mulfinger; Sabine Müller; Isabel Böge; Vehbi Sakar; Patrick W. Corrigan; Sara Evans-Lacko; Luise Nehf; Julia Djamali; Anna Samarelli; Michael Kempter; Christian Ruckes; Gerhard Libal; Nathalie Oexle; Michele Noterdaeme; Nicolas Rüsch. Honest, Open, Proud for adolescents with mental illness: pilot randomized controlled trial. Journal of Child Psychology and Psychiatry 2017, 59, 684 -691.

AMA Style

Nadine Mulfinger, Sabine Müller, Isabel Böge, Vehbi Sakar, Patrick W. Corrigan, Sara Evans-Lacko, Luise Nehf, Julia Djamali, Anna Samarelli, Michael Kempter, Christian Ruckes, Gerhard Libal, Nathalie Oexle, Michele Noterdaeme, Nicolas Rüsch. Honest, Open, Proud for adolescents with mental illness: pilot randomized controlled trial. Journal of Child Psychology and Psychiatry. 2017; 59 (6):684-691.

Chicago/Turabian Style

Nadine Mulfinger; Sabine Müller; Isabel Böge; Vehbi Sakar; Patrick W. Corrigan; Sara Evans-Lacko; Luise Nehf; Julia Djamali; Anna Samarelli; Michael Kempter; Christian Ruckes; Gerhard Libal; Nathalie Oexle; Michele Noterdaeme; Nicolas Rüsch. 2017. "Honest, Open, Proud for adolescents with mental illness: pilot randomized controlled trial." Journal of Child Psychology and Psychiatry 59, no. 6: 684-691.