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Background: The prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales (ESBL-E) and carbapenemase-producing Enterobacterales (CPE) is now disseminated worldwide. This study aims to describe the prevalence of ESBL and CPE fecal carriage in colorectal cancer patients.Methods: All patients admitted to the oncology service of Amizour hospital (Algeria) for colorectal cancer chemotherapy from March to May 2019 were screened for ESBL-E or CPE fecal carriage. After culturing on chromogenic media, the presumptive colonies were identified by mass spectroscopy. Antibiotic susceptibility testing was performed according to the European Committee on Antimicrobial Susceptibility Testing. The β-lactamases encoding genes and plasmid-mediated quinolone-resistant genes were screened by PCR and sequencing.Results: ESBL-E strains were recovered from rectal swabs in 6 patients (14.3%) and only 1 patient (2.4%) was found a carrier for OXA-48-producing Klebsiella pneumoniae. The most frequently encountered species among ESBL-E was Escherichia coli (n = 5), followed by K. pneumoniae (n = 1). PCR and sequencing showed that four isolates harbored the blaCTX-M-15 gene and two strains harbored the blaCTX-M-14 gene. Also, one strain of K. pneumoniae was found to harbor both qepA and qnrS genes.Conclusion: This study highlighted the fecal carriage of ESBL-E and OXA-48-producing Enterobacterales strains in colorectal cancer patients.
Abdelaziz Touati; Melissa Talbi; Assia Mairi; Abdelaziz Messis; Ahmed Adjebli; Mustapha Louardiane; Jean Philippe Lavigne. Fecal Carriage of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Enterobacterales Strains in Patients with Colorectal Cancer in the Oncology Unit of Amizour Hospital, Algeria: A Prospective Cohort Study. Microbial Drug Resistance 2020, 26, 1383 -1389.
AMA StyleAbdelaziz Touati, Melissa Talbi, Assia Mairi, Abdelaziz Messis, Ahmed Adjebli, Mustapha Louardiane, Jean Philippe Lavigne. Fecal Carriage of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Enterobacterales Strains in Patients with Colorectal Cancer in the Oncology Unit of Amizour Hospital, Algeria: A Prospective Cohort Study. Microbial Drug Resistance. 2020; 26 (11):1383-1389.
Chicago/Turabian StyleAbdelaziz Touati; Melissa Talbi; Assia Mairi; Abdelaziz Messis; Ahmed Adjebli; Mustapha Louardiane; Jean Philippe Lavigne. 2020. "Fecal Carriage of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Enterobacterales Strains in Patients with Colorectal Cancer in the Oncology Unit of Amizour Hospital, Algeria: A Prospective Cohort Study." Microbial Drug Resistance 26, no. 11: 1383-1389.
This review assessed the molecular characterization of the methicillin-resistant Staphylococcus aureus (MRSA)-ST80 clone with an emphasis on its proportion of total MRSA strains isolated, PVL production, spa-typing, antibiotic resistance, and virulence. A systematic review of the literature was conducted on MRSA-ST80 clone published between 1 January 2000 and 31 August 2019. Citations were chosen for a review of the full text if we found evidence that MRSA-ST80 clone was reported in the study. For each isolate, the country of isolation, the sampling period, the source of isolation (the type of infection, nasal swabs, or extra-human), the total number of MRSA strains isolated, number of MRSA-ST80 strains, antibiotic resistance patterns, PVL production, virulence genes, and spa type were recorded. The data from 103 articles were abstracted into an Excel database. Analysis of the data showed that the overall proportion of MRSA-ST80 has been decreasing in many countries in recent years. The majority of MRSA-ST80 were PVL positive with spa-type t044. Only six reports of MRSA-ST80 in extra-human niches were found. This review summarizes the rise of MRSA-ST80 and the evidence that suggests that it could be in decline in many countries.
Assia Mairi; Abdelaziz Touati; Jean-Philippe Lavigne. Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review. Toxins 2020, 12, 119 .
AMA StyleAssia Mairi, Abdelaziz Touati, Jean-Philippe Lavigne. Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review. Toxins. 2020; 12 (2):119.
Chicago/Turabian StyleAssia Mairi; Abdelaziz Touati; Jean-Philippe Lavigne. 2020. "Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review." Toxins 12, no. 2: 119.
The diffusion of Panton-Valentine leukocidin (PVL)-positive methicillin-resistant S. aureus (MRSA) is a health problem in Algeria. The objectives of the study were to investigate the global distribution of methicillin-susceptible S. aureus (MSSA) and MRSA isolates in different ecological niches in this country. In total, 2246 samples were collected from humans, livestock, wild animals, pets, food products and the aquatic environment, from 12 Algerian provinces. A total of 312 S. aureus were detected from 2446 samples (12.7%) in the screened niches. We observed the emergence of toxinogenic S. aureus representing 41% of the isolates. Among them, we noted the diffusion of ST80-IV CA-MRSA PVL + strains isolated in human, animals, and food and genetic diversity of MSSA PVL + isolates. This study suggests an alarming dissemination of MRSA-ST80 PVL + in both human and extra-human sources in Algeria. Moreover, MSSA may become a permanent reservoir of the PVL genes necessary for human infections.
Assia Mairi; Abdelaziz Touati; Alix Pantel; Karima Zenati; Alex Yahiaoui Martinez; Catherine Dunyach-Remy; Albert Sotto; Jean-Philippe Lavigne. Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria. Toxins 2019, 11, 500 .
AMA StyleAssia Mairi, Abdelaziz Touati, Alix Pantel, Karima Zenati, Alex Yahiaoui Martinez, Catherine Dunyach-Remy, Albert Sotto, Jean-Philippe Lavigne. Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria. Toxins. 2019; 11 (9):500.
Chicago/Turabian StyleAssia Mairi; Abdelaziz Touati; Alix Pantel; Karima Zenati; Alex Yahiaoui Martinez; Catherine Dunyach-Remy; Albert Sotto; Jean-Philippe Lavigne. 2019. "Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria." Toxins 11, no. 9: 500.
Introduction: The spread of carbapenemase-producing Enterobacteriaceae (CPE) represents a major public health issue. Methods allowing rapid detection of carbapenemases in developing countries are therefore urgently needed. In the current study, we developed a new in-house medium for the rapid detection of CPE isolates, especially OXA-48 producers. Methodology: A panel of 144 clinical strains previously characterized was tested on in-house Carba MTL-broth medium using four different concentrations of ertapenem (0.5 to 2 mg/L), and compared to chromID® OXA-48 and chromID® CARBA (BioMérieux) media. Results: Comparative evaluation of the Carba MTL-broth with chromID® OXA-48 and chromID® CARBA showed that chromID® OXA-48 and Carba MTL-broth had the highest sensitivity for detection of OXA-48 producers (93.9% and 100%, respectively) comparatively to chromID® CARBA (21.2%). The chromID® OXA-48 had the highest specificity (100%), as compared to the Carba MTL-broth (65.5%) and chromID® CARBA (84.4%) for the detection of OXA-48 producers. Conclusions: The in-house Carba MTL-broth developed in this study is sensitive, inexpensive, an easy-to-use phenotypic method for the detection of OXA-48-producing enterobacteria. Given the burden of pan-drug resistance, its implementation in the microbiology laboratory of developing countries could be a useful tool for rapid detection of these bacteria.
Assia Mairi; Abdelaziz Touati; Alix Pantel; Catherine Dunyach-Remy; Albert Sotto; Christophe De Champs; Jean-Philippe Lavigne. Performance of a new in-house medium Carba MTL-broth for the rapid detection of carbapenemase-producing Enterobacteriaceae. The Journal of Infection in Developing Countries 2019, 13, 591 -602.
AMA StyleAssia Mairi, Abdelaziz Touati, Alix Pantel, Catherine Dunyach-Remy, Albert Sotto, Christophe De Champs, Jean-Philippe Lavigne. Performance of a new in-house medium Carba MTL-broth for the rapid detection of carbapenemase-producing Enterobacteriaceae. The Journal of Infection in Developing Countries. 2019; 13 (7):591-602.
Chicago/Turabian StyleAssia Mairi; Abdelaziz Touati; Alix Pantel; Catherine Dunyach-Remy; Albert Sotto; Christophe De Champs; Jean-Philippe Lavigne. 2019. "Performance of a new in-house medium Carba MTL-broth for the rapid detection of carbapenemase-producing Enterobacteriaceae." The Journal of Infection in Developing Countries 13, no. 7: 591-602.