Jeff Lines

Dr. Jeff Lines


Research Focus & Metrics

Academic Career Length

16Years (2011-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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12 October 2011 in Fish Physiology and Biochemistry
Verified
Journal Article
Closed access
PDF file
JLJeff Lines
Jeff Lines
JSJ. Spence
J. Spence

20 April 2018 in Animals
Verified
Journal Article
Open access
PDF file
AGAndrew Grist
Andrew Grist
JLJeff Lines
Jeff Lines
TKToby G. Knowles
Toby G. Knowles
CMCharles W. Mason
Charles W. Mason
SWSteve Wotton
Steve Wotton
A non-penetrating captive bolt device, powered by a 1 grain 0.22″ cartridge delivering a calculated kinetic energy of 47 Joules was tested as a euthanasia method on 200 neonate goats (Capra aegagrus hircus) of mean dead weight = 4.425 kg (SD (Standard deviation) ± 0.4632), to assess effectiveness and shot position. Evaluation of the method was conducted using behavioural indicators of brain dysfunction followed by post mortem examination of the heads. Once correct shot position had been established, 100% of 158 kids (95% confidence interval 97.5% to 100%) were successfully stunned/killed with a shot positioned on the midline, between the ears, with the chin tucked into the neck. The use of the Accles and Shelvoke CASH Small Animal Tool can therefore be recommended for the euthanasia of neonate goats with a 1 grain cartridge and a specific shooting position.

2 April 2018 in Animals
Verified
Journal Article
Open access
PDF file
AGAndrew Grist
Andrew Grist
JLJeff Lines
Jeff Lines
TKToby G. Knowles
Toby G. Knowles
CMCharles W. Mason
Charles W. Mason
SWSteve Wotton
Steve Wotton
The most common method for the on-farm euthanasia of neonate piglets is reported to be manual blunt force trauma. This paper presents the results of research to evaluate a mechanical non-penetrating captive bolt (the Accles and Shelvoke CASH small animal tool, Birmingham, UK) to produce an immediate stun/kill with neonate piglets. One hundred and forty-seven piglets (average dead weight = 1.20 kg ± 0.58 (standard deviation, SD), mean age = 5.8 days (median = 3)) were euthanized with the device and demonstrated immediate loss of consciousness, subjectively assessed by behavioural signs and no recovery. The result that 147 out of 147 animals were effectively stun/killed gives a 95% confidence interval for the true percentage of animals that would be effectively stun/killed of 97.5–100% with the use of the CASH small animal tool under the conditions of the current study. This research concludes that the CASH small animal tool, using a 1 grain brown coded cartridge, is suitable for producing a stun/kill in neonate piglets when applied in a frontal/parietal position.

2 April 2018 in Animals
Verified
Journal Article
Open access
PDF file
AGAndrew Grist
Andrew Grist
JLJeff Lines
Jeff Lines
TKToby G. Knowles
Toby G. Knowles
CMCharles W. Mason
Charles W. Mason
SWSteve Wotton
Steve Wotton
A non-penetrating captive bolt device, powered by a 1-grain 0.22″ cartridge delivering a calculated kinetic energy of 47 Joules was tested as a euthanasia method on 200 neonate lambs (Ovis aries) of 4.464 kg (SD (Standard deviation) ± 1.056) mean dead weight, to assess effectiveness and shot position. Every lamb (n = 200) was effectively stunned when the weapon was applied powered by a brown, 1-grain cartridge but 10/200 (5%) of the lambs displayed rhythmic or agonal breathing and were subsequently euthanased using euthatal (Merial, UK, GTIN: 03661103015550). Evaluation of the method was conducted using behavioural indicators of brain dysfunction followed by post-mortem examination of the heads. A second trial was conducted using a higher velocity 1.25-grain cartridge and a specific shot position on 48 lambs (mean dead weight = 6.21 kg, SD ± 1.24) averaging 5 days old. One hundred percent of the lambs in the second trial were immediately stun-killed. Given this complete kill rate and the sample size of the study, the study provides a statistical 95% confidence interval of 92.6% to 100%. The use of the Accles & Shelvoke “CASH” Small Animal Tool (Birmingham, UK) can therefore be recommended for the euthanasia of neonate lambs with a 1.25-grain cartridge and a specific shooting position.

1 August 2011 in British Poultry Science
Verified
Journal Article
Closed access
JLJeff Lines
Jeff Lines
ARA.B.M. Raj
A.B.M. Raj
SWS.B. Wotton
S.B. Wotton
MOM. O’cAllaghan
M. O’cAllaghan
TKT.G. Knowles
T.G. Knowles
1. The objective of this work was to investigate the feasibility of head only waterbath stunning as a means of generating immediate and long lasting unconsciousness while preventing wing flapping and avoiding carcass damage. 2. EEG measurements showed that immersion of the heads of the broilers for one second in a waterbath containing water of conductivity 2·5 mS/cm and a 50 Hz electric field of 10 V/cm resulted in immediate unconsciousness, and that increasing the electric field strength extended the duration of unconsciousness. 3. The passage of a 25–30 mA alternating current of frequency 2000 Hz through the broilers’ bodies suppressed the wing flapping that followed a stun. 4. When the body current and electric field were applied simultaneously, wing flapping was prevented and EEG signals were suppressed for over 30 seconds indicating that the immediate unconsciousness lasted long enough to facilitate humane slaughter.

1 August 2011 in British Poultry Science
Verified
Journal Article
Closed access
JLJeff Lines
Jeff Lines
SWS.B. Wotton
S.B. Wotton
RBR. Barker
R. Barker
JSJ. Spence
J. Spence
LWL. Wilkins
L. Wilkins
TKT.G. Knowles
T.G. Knowles
1. The objective was to assess carcass quality of broilers when they were stunned by immersing their heads in a waterbath with an electric current flowing from one side of it to the other, while a second small current passed through the body to the waterbath to prevent involuntary wing flapping. 2. The prevalence of wing, shoulder and breast fillet haemorrhages and of broken bones in the pectoral region was not greater than that resulting from the normal stunning practice in that plant (63 mA, 610 Hz pDC). 3. These results imply that carcass damage using this technique will be significantly lower than that which will result from the application of higher stunning currents required by the new EU slaughter poultry slaughter regulations.