Due to unique physicochemical properties, magnesium oxide nanoparticles (MgO NPs) have shown great potential for various applications, including biomedical and environmental remediation. Moreover, the physiochemical properties of MgO NPs can be tailored by metal ion doping that can be utilized in photocatalytic performance and in the biomedical field. There is limited study on the photocatalytic activity and biocompatibility of silver (Ag)-doped MgO NPs. This study was planned for facile synthesis, characterization, and photocatalytic activity of pure and silver (Ag)-doped MgO NPs. In addition, cytotoxicity of pure and Ag-doped MgO NPs was assessed in human normal umbilical vein endothelial cells (HUVECs). Pure MgO NPs and Ag-doped (1, 2, 5, and 7.5 mol%) MgO NPs were prepared via a simple sol-gel procedure. X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), photoluminescence (PL), and X-ray photoelectron spectroscopy (XPS) were used to characterize the prepared samples. XRD results showed the preparation of highly crystalline NPs with no impurity peaks. TEM and SEM studies indicate smooth surfaces with almost spherical morphology of MgO NPs, and Ag-doping did not change the morphology. Elemental composition study suggested that Ag is uniformly distributed in MgO particles. Intensity of the PL spectra of MgO NPs decreased with increasing the concentration of Ag dopants. In comparison to pure MgO NPs, Ag-MgO NPs showed higher degradation of methylene blue (MB) dye under UV irradiation. The improved photocatalytic activity of Ag-MgO NPs was related to the effect of dopant concentration on reducing the recombination between electrons and holes. Cytotoxicity studies showed good biocompatibility of pure and Ag-doped MgO NPs with human normal umbilical vein endothelial cells (HUVECs). These results highlighted the potential of Ag-doped MgO NPs in environmental remediation.

Metal oxide and graphene derivative-based nanocomposites (NCs) are attractive to the fields of environmental remediation, optics, and cancer therapy owing to their remarkable physicochemical characteristics. There is limited information on the environmental and biomedical applications of tin oxide-reduced graphene oxide nanocomposites (SnO₂-rGO NCs). The goal of this work was to explore the photocatalytic activity and anticancer efficacy of SnO₂-rGO NCs. Pure SnO₂ NPs and SnO₂-rGO NCs were prepared using the one-pot hydrothermal method. X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR), UV–Vis spectrometry, photoluminescence (PL), and Raman scattering microscopy were applied to characterize the synthesized samples. The crystallite size of the SnO₂ NPs slightly increased after rGO doping. TEM and SEM images show that the SnO₂ NPs were tightly anchored onto the rGO sheets. The XPS and EDX data confirmed the chemical state and elemental composition of the SnO₂-rGO NCs. Optical data suggest that the bandgap energy of the SnO₂-rGO NCs was slightly lower than for the pure SnO₂ NPs. In comparison to pure SnO₂ NPs, the intensity of the PL spectra of the SnO₂-rGO NCs was lower, indicating the decrement of the recombination rate of the surfaces charges (e⁻/h⁺) after rGO doping. Hence, the degradation efficiency of methylene blue (MB) dye by SnO₂-rGO NCs (93%) was almost 2-fold higher than for pure SnO₂ NPs (54%). The anticancer efficacy of SnO₂-rGO NCs was also almost 1.5-fold higher against human liver cancer (HepG2) and human lung cancer (A549) cells compared to the SnO₂ NPs. This study suggests a unique method to improve the photocatalytic activity and anticancer efficacy of SnO₂ NPs by fusion with graphene derivatives.

Graphene-based nanocomposites have attracted enormous interest in nanomedicine and environmental remediation, owing to their unique characteristics. The increased production and widespread application of these nanocomposites might raise concern about their adverse health effects. In this study, for the first time, we examine the cytotoxicity and oxidative stress response of a relatively new nanocomposite of cerium oxide-reduced graphene oxide (CeO₂-RGO) in human lung epithelial (A549) cells. CeO₂-RGO nanocomposites and RGO were prepared by a simple hydrothermal method and characterized by relevant analytical techniques. Cytotoxicity data have shown that RGO significantly induces toxicity in A549 cells, evident by cell viability reduction, membrane damage, cell cycle arrest, and mitochondrial membrane potential loss. However, CeO₂-RGO nanocomposites did not cause statistically significant toxicity as compared to a control. We further observed that RGO significantly induces reactive oxygen species generation and reduces glutathione levels. However, CeO₂-RGO nanocomposites did not induce oxidative stress in A549 cells. Interestingly, we observed that CeO₂ nanoparticles (NPs) alone significantly increase glutathione (GSH) levels in A549 cells as compared to a control. The GSH replenishing potential of CeO₂ nanoparticles could be one of the possible reasons for the biocompatible nature of CeO₂-RGO nanocomposites. Our data warrant further and more advanced research to explore the biocompatibility/safety mechanisms of CeO₂-RGO nanocomposites in different cell lines and animal models.

The aim of the present study is to enhance the anticancer and biocompatibility performance of TiO ₂ and ZnO nanoparticles, and, ZnO-TiO ₂ and ZnO-TiO ₂ /reduced graphene oxide nanocomposites, against two types of human cancer (HCT116) and normal (HUVCE) cells.

The incorporation of graphene with metal oxide has been widely explored in various fields, including energy storage devices, optical applications, biomedical applications, and water remediation. This research aimed to assess the impact of reduced graphene oxide (RGO) doping on the photocatalytic and anticancer properties of In₂O₃ nanoparticles. Pure and In₂O₃/RGO nanocomposites were effectively synthesized using the single-step microwave hydrothermal process. XRD, TEM, SEM, EDX, XPS, Raman, UV–Vis, and PL spectroscopy were carefully utilized to characterize the prepared samples. XRD data showed that synthesized In₂O₃ nanoparticles had high crystallinity with a decreased crystal size after RGO doping. TEM and SEM images revealed that the In₂O₃ NPs were spherical and uniformly embedded onto the surface of RGO sheets. Elemental analysis of In₂O₃/RGO NC confirmed the presence of In, O, and C without impurities. Raman analysis indicated the successful fabrication of In₂O₃ onto the RGO surface. Uv–Vis analysis showed that the band gap energy was changed with RGO addition. Raman spectra confirmed that In₂O₃ nanoparticles were successfully anchored onto the RGO sheet. PL results indicated that the prepared In₂O₃/RGO NCs can be applied to enhance photocatalytic activity and biomedical applications. In the degradation experiment, In₂O₃/RGO NCs exhibited superior photocatalytic activity compared to that of pure In₂O₃. The degradation efficiency of In₂O₃/RGO NCs for MB dye was up to 90%. Biological data revealed that the cytotoxicity effect of In₂O₃/RGO NCs was higher than In₂O₃ NPs in human colorectal (HCT116) and liver (HepG2) cancer cells. Importantly, the In₂O₃/RGO NCs exhibited better biocompatibility against human normal peripheral blood mononuclear cells (PBMCs). All the results suggest that RGO addition improves the photocatalytic and anticancer activity of In₂O₃ NPs. This study highlights the potential of In₂O₃/RGO NCs as an efficient photocatalyst and therapeutic material for water remediation and biomedicine.

This study aimed to generate a comparative data on biological response of yttrium oxide nanoparticles (Y₂O₃ NPs) with the antioxidant CeO₂ NPs and pro-oxidant ZnO NPs. Sizes of Y₂O₃ NPs were found to be in the range of 35±10 nm as measured by TEM and were larger from its hydrodynamic sizes in water (1004 ± 134 nm), PBS (3373 ± 249 nm), serum free culture media (1735 ± 305 nm) and complete culture media (542 ± 108 nm). Surface reactivity of Y₂O₃ NPs with bovine serum albumin (BSA) was found significantly higher than for CeO₂ and ZnO NPs. The displacement studies clearly suggested that adsorption to either BSA, filtered serum or serum free media was quite stable, and was dependent on whichever component interacted first with the Y₂O₃ NPs. Enzyme mimetic activity, like that of CeO₂ NPs, was not detected for the NPs of Y₂O₃ or ZnO. Cell viability measured by MTT and neutral red uptake (NRU) assays suggested Y₂O₃ NPs were not toxic in human breast carcinoma MCF-7 and fibroblast HT-1080 cells up to the concentration of 200 μg/mL for a 24 h treatment period. Oxidative stress markers suggested Y₂O₃ NPs to be tolerably non-oxidative and biocompatible. Moreover, mitochondrial potential determined by JC-1 as well as lysosomal activity determined by lysotracker (LTR) remained un-affected and intact due to Y₂O₃ and CeO₂ NPs whereas, as expected, were significantly induced by ZnO NPs. Hoechst-PI dual staining clearly suggested apoptotic potential of only ZnO NPs. With high surface reactivity and biocompatibility, NPs of Y₂O₃ could be a promising agent in the field of nanomedicine.