At high altitudes, hypoxia, oxidative stress or both compromise sheep fertility. In the present work, we tested the relative effect of short- or long-term exposure to high altitude hypobaric hypoxia and oxidative stress on corpora luteal structure and function.

The aim of the present study was to assess the reliability of fecal indices as predictors of nutrient intake in sheep under extensive grazing conditions. Fecal concentrations of 2,6-diaminopimelic acid (DAPAf), nitrogen (FN) and phosphorous (FP) were determined in four sheep kept in an extensive grazing system on annual Mediterranean grassland in the vegetative, reproductive and dry phenological stages. Metabolizable energy (MEI), crude protein (CPI) and phosphorus (PI) intake were calculated using the botanical composition, metabolizable energy, crude protein and phosphorus concentrations in each vegetal species making up the animal’s diet. Significant differences were observed in the nutrient intake for each phenological stage (p < 0.0001). The highest MEI, CPI and PI were observed during the vegetative stage (p < 0.0001). FN and FP were different in each phenological stage (p < 0.0001), with significant correlations observed between these variables (r = 0.916; p < 0.0001). Regressions among nutrient intake and fecal indices were significant, except in the cases of DAPAf and MEI, and DAPAf and CPI. Based on these results, fecal indices could be used to estimate nutrient intake in sheep under extensive grazing on annual Mediterranean grassland.

For an adequate management of natural grasslands, the knowledge and understanding of the dietary habits of herbivores and their trophic interactions are fundamental. During two summer seasons, in a mountain range of a sector of the Coquimbo Region, Chile, the botanical composition, diversity, and similarity of the diets of horses, European brown hares, and guanacos were studied, as was the selectivity of the main grassland plant species, using feces microhistology. The contribution of hydromorphic grasses was similar in the diets of guanacos (35.90 ± 7.27%) and horses (32.25 ± 4.50%), differing from that found in hares (16.32 ± 5.32%). Dryland grassland grasses contributed similarly to the diets of horses (13.21 ± 3.22%), guanacos (22.53 ± 5.21%) and hares (18.35 ± 3.81%), as well as graminoids, which averaged 47.79 ± 6.66%, 35.63 ± 10.76% and 38.94 ± 7.88%, in diets of horses, guanacos, and hares, respectively, without significant differences. The contribution of herbaceous dicotyledons was only important in hares (23.76 ± 3.76%), while that of shrubs was low (<3%) and similar among the three herbivores. Dietary diversity was similar among the three herbivore species (73–79%), with a higher degree of dietary overlap between horses and guanacos (55.7%), which was higher than that obtained between hares and guanacos (50%) and between horses and hares (48%), for which there would be a potential trophic competition between them. The most abundant species of dryland and wet grasslands generally fulfill a functional role of subsistence and a nutritional role of maintenance; however, for the three herbivores studied, a different selective behavior was evidenced, according to their physiological differences, with the selection process little affected by the relative abundance of these species in the grasslands. Due to the above, herbivores resort to the selection of certain species that, despite being not very abundant in grasslands, play an important nutritional and functional role, improving the quality of their diets.

Understanding the botanical composition of herbivores’ diets and their nutritional quality is an important question in the development of sustainable strategies for the management of natural resources. In Chilean highland vicuña-grazed grasslands, there is little information in this regard and, therefore, this study aimed to determine the year-round profile of the diet’s botanical composition and quality. In highland grasslands, on an area of 21.9 ha, continuously grazed for 3.06 VU/ha/year (18°03′ S, 69°13′ W; 4425 m.o.s.l), twelve feces piles were sampled monthly and were analyzed through microhistology, and the nitrogen concentration [NF, OM basis] was determined. The botanical composition, diversity (J) and selectivity index (E_i) of the main species were estimated. Diets were composed of dry–grassland grasses (37.7%), wet–grassland grasses (36.6%), graminoids (14.3%) and forbs (10.2%). The diet diversity ranged from 0.79 (dry–winter) to 0.87 (wet–summer). The main dominant grassland species obtained negative E_i values. The annual mean value of [NF] was estimated as 1.82%, with a higher value in summer months (2.21%), which coincides with the physiological states of higher nutritional demand. The vicuñas behave like generalist ungulates, having a high degree of selectivity towards grass species, which mostly fulfill a nutritional role in subsistence and a functional role in survival, applying foraging strategies that allow them to obtain a better quality diet during the season of greatest nutritional demand.

The aim of the present study was to evaluate the effect of two types of nutritional supplementation during late gestation on the chemical composition, energy value, and IgG concentration in the colostrum and the IgG concentration in the blood serum of lambs. Pregnant Merino Precoz ewes (n = 36) carrying single fetuses were used. Animals were kept grazing on the Mediterranean annual grassland. From day ~90 of pregnancy, animals were allocated into three groups: daily supplementation with oat grain or lupine grain and a control group without supplementation. Immediately after parturition, colostrum was collected from each ewe, and a blood sample was taken from the lambs 24 h after birth. For the evaluation of the chemical composition of the colostrum, an EKOMILK^® milk analyzer was used. The energy value of the colostrum was calorimetrically evaluated. IgG concentrations were measured by simple radial immunodiffusion. Data were analyzed by analysis of variance. Colostrum content of protein and non-fat solids was higher in the group supplemented with oat grain than in the lupine grain supplemented and control groups (p ≤ 0.05). In contrast, ewes supplemented with lupine grain had the highest concentration of fat in their colostrum (p ≤ 0.05). Oat grain supplementation resulted in higher concentrations of IgG, both in sheep colostrum and in the blood serum of their lambs (p ≤ 0.05), being higher than those observed in the lupine grain and control groups. Ewes that gave birth to male lambs had significantly higher concentrations of IgG in their colostrum compared to ewes that gave birth to females (p ≤ 0.05). The colostral IgG concentration positively correlated with the serum IgG concentration of the lambs (r = 0.32; p ≤ 0.05). The results indicate that the quality of colostrum and the immunological status of the newborn lambs can be improved by supplementation with oat grain.