This page has only limited features, please log in for full access.
Award for the best research paper in the 10th Arab Congress of Plant Protection, 26-30 October, 2009, Beirut, Lebanon
10th Arab Congress of Plant Protection
Award of SCIENCEDIRECT TOP 25 research article provided by Elsevier B.V., Amsterdam, The Netherlands 2014 (Sodium carbonate and bicarbonate treatments induce resistance to postharvest green mold on citrus fruit. Postharvest Biology and Technology, 87: 61-69) http://dx.doi.org/10.1016/j.postharvbio.2013.08.006
Elsevier B.V., Amsterdam, The Netherlands
Dr. Youssef Khamis was born in Egypt in 1980, he obtained his BSc in 2002 of Agriculture Sciences, Plant Pathology from Faculty of Agriculture, Assiut University, Egypt. In 2005, he got the DSPU in “IPM of Mediterranean Fruit Tree Crops”. In 2007, he obtained his MSc at CIHEAM-MAIB, Italy. In 2010, he had a training course at Cranfield University (UK). In 2011, he got his PhD in Plant Pathology from Bari University Aldo Moro, Italy. Now he is working as a Research Plant Pathologist in Egypt.
RNAi technique was used to regulate Fusarium crown and root rot in tomatoes caused by F. oxysporum f. sp. radicis-lycopersici (FORL), by targeting three essential genes namely FoCYP51, FoChs1, and FoEF2, considering the fungicide site of action. The designed naked dsRNA was delivered on a hexagonal mono-dispersed and biodegradable layered double hydroxide (LDH) nanosheets with 30–90 nm diameter, providing high protection and stability for dsRNA with long term sustained release manner inside plant cells. The laboratory findings revealed the functional role of the three selected genes in reducing FORL’s invasive growth on tomato fruits, indicating their involvement in FORL pathogenesis on tomatoes, and could be good targets for disease control using RNAi. Three different practical methods were applied for topical delivery of dsRNA into the plant cells: i) leaves spray; ii) petiole adsorption; and iii) root dipping, demonstrating three significant levels of spray-induced gene silencing (SIGS) efficacy in controlling FORL virulence infecting tomato plants. Significantly, topical spray of dsRNA delivered on LDH nanosheets provided Fusarium crown and root rot protection for at least 60 days. Obtained findings introduce critical information data on the potential to exploit spray-induced gene silencing (SIGS) as an effective alternative sustainable strategy for plant disease management. Given the ease design and production of biodegradable nano-delivery systems with high specificity and sustainability, and ease of applicability against many disease caused fungi. The use of target-specific dsRNA as an anti-fungal agent has a lot of promise and provide exceptional potential as a unique disease control and plant protection strategy.
Mosa A. Mohamed; Khamis Youssef. Topical delivery of host induced RNAi silencing by layered double hydroxide nanosheets: an efficient tool to decipher pathogenicity gene function of Fusarium crown and root rot in tomato. Physiological and Molecular Plant Pathology 2021, 101684 .
AMA StyleMosa A. Mohamed, Khamis Youssef. Topical delivery of host induced RNAi silencing by layered double hydroxide nanosheets: an efficient tool to decipher pathogenicity gene function of Fusarium crown and root rot in tomato. Physiological and Molecular Plant Pathology. 2021; ():101684.
Chicago/Turabian StyleMosa A. Mohamed; Khamis Youssef. 2021. "Topical delivery of host induced RNAi silencing by layered double hydroxide nanosheets: an efficient tool to decipher pathogenicity gene function of Fusarium crown and root rot in tomato." Physiological and Molecular Plant Pathology , no. : 101684.
The two-spotted spider mite (TSSM) Tetranychus urticae is a polyphagous pest which infests several wild and cultivated species of plants worldwide. To date, this mite is the most deleterious pest attacking the strawberry plant under a protected environment. Exogenously applied salicylic acid (SA) has been found to induce resistance to biotic and abiotic stresses. Morpho-anatomical changes in strawberry leaflets and TSSM preference tests were investigated in response to SA treatment. Plants grown in a greenhouse were foliar sprayed with different concentrations of SA (0 mg · L−1, 25 mg · L−1, 50 mg · L−1, 75 mg · L−1 and 100 mg · L−1). After the third application, certain parameters including the number of glandular and non-glandular trichomes, thickness of leaflet, abaxial cell wall, adaxial cell wall, palisade and lacunous parenchyma and mesophyll were measured using scanning electronic microscopy (SEM). Two-choice and non-choice assays were employed to verify the TSSM preference. Exogenous treatment with SA promoted morpho-anatomical changes in the following parameters, namely: thickness of the leaflets, mesophyll, lacunous and palisade parenchyma, cell wall (abaxial and adaxial) and the number of glandular and non-glandular trichomes in strawberry leaflets. In general, TSSM preferred less leaflets treated with SA compared with the control in a two-choice assay. A lesser number of TSSM eggs and live females were also recorded in leaflets treated with SA compared with the control plants in no-choice assays. The values of the number of eggs and live females correlated negatively with those obtained for the morpho-anatomical traits induced by exogenous SA.
Juliano T. Vilela de Resende; Rafael Matos; Douglas M. Zeffa; Leonel Vinicius Constantino; Silas M. Alves; Maurício U. Ventura; Nathalia C. V. Resende; Khamis Youssef. Relationship between salicylic acid and resistance to mite in strawberry. Folia Horticulturae 2021, 1 .
AMA StyleJuliano T. Vilela de Resende, Rafael Matos, Douglas M. Zeffa, Leonel Vinicius Constantino, Silas M. Alves, Maurício U. Ventura, Nathalia C. V. Resende, Khamis Youssef. Relationship between salicylic acid and resistance to mite in strawberry. Folia Horticulturae. 2021; ():1.
Chicago/Turabian StyleJuliano T. Vilela de Resende; Rafael Matos; Douglas M. Zeffa; Leonel Vinicius Constantino; Silas M. Alves; Maurício U. Ventura; Nathalia C. V. Resende; Khamis Youssef. 2021. "Relationship between salicylic acid and resistance to mite in strawberry." Folia Horticulturae , no. : 1.
Chemical insecticides comprise the most widely used method of tomato pest control. Genotypes resistant to pests such as the silver leaf whitefly Bemisia tabaci may contribute towards the decreased use of insecticides to protect the environment. Leaf trichomes play a role in imparting resistance to pests, and exploiting intraspecific and interspecific variability for leaf trichomes in the tomato gene pool is an alternative to speed up introgression of resistance into improved lines or cultivars. We assessed the levels of whitefly resistance and trichome morphology/density in an array of accessions of the tomato gene pool and their hybrids with a tomato cultivar. A commercial cultivar (S. lycopersicum cv. Redenção), six wild species accessions, two S. lycopersicum var. cerasiforme genotypes, and eight intra and interspecific F1 hybrids were tested. The accessions LA 1401 (S. galapagense), AF 19684 (S. peruvianum), PI 127826 (S. habrochaites var. hirsutum), PI 134417 (S. habrochaites var. glabratum), LA 716 (S. pennellii), and RVTC 03 (S. lycopersicum var. cerasiforme) genotypes showed a higher density of glandular trichomes and carried the highest levels of whitefly resistance than the commercial cultivar. Segregating generations from their crosses with the cultivar may be promising sources to select plants with both higher density of glandular trichomes and resistance to whiteflies. Populations derived from S. lycopersicum var. cerasiforme’ RVTC 03’, which is part of intraspecific S. lycopersicum variability, maybe a more convenient source of pest resistance than those derived from interspecific crosses to obtain pest-resistant tomato cultivars.
André Ricardo Zeist; Juliano Tadeu Vilela de Resende; Amanda Carvalho Perrud; André Gabriel; Wilson Roberto Maluf; José Henrique Verhalem Arantes; Khamis Youssef. Resistance to Bemisia tabaci in tomato species and hybrids and its association with leaf trichomes. Euphytica 2021, 217, 1 -10.
AMA StyleAndré Ricardo Zeist, Juliano Tadeu Vilela de Resende, Amanda Carvalho Perrud, André Gabriel, Wilson Roberto Maluf, José Henrique Verhalem Arantes, Khamis Youssef. Resistance to Bemisia tabaci in tomato species and hybrids and its association with leaf trichomes. Euphytica. 2021; 217 (5):1-10.
Chicago/Turabian StyleAndré Ricardo Zeist; Juliano Tadeu Vilela de Resende; Amanda Carvalho Perrud; André Gabriel; Wilson Roberto Maluf; José Henrique Verhalem Arantes; Khamis Youssef. 2021. "Resistance to Bemisia tabaci in tomato species and hybrids and its association with leaf trichomes." Euphytica 217, no. 5: 1-10.
The aim of this work was to evaluate different SO2-generating pads and liners to control gray mold in ventilated clamshell-packaged ‘Rubi’ table grapes grown under a two-cropping per year system. The treatments consisted of SO2-generating pads (slow release or dual release) and plastic liners with different perforations (microperforated; 2.0; 4.0 or 5.0 mm in diameter) and a control, only with the standard microperforated plastic liner. The packaged grapes were stored in a cold chamber at 1.0 ± 1.0 °C and 95% relative humidity. After 45 days, the grapes were removed from cold storage and placed, without liners and SO2-generating pads, for 3 days at room temperature (22.0 ± 1.0 °C). The evaluations occurred at 30 and 45 days after the beginning of cold storage, and the following variables were assessed: incidence of gray mold, mass loss, stem browning and shattered berries. At 3 days of shelf-life, the same variables were assessed, except mass loss. The completely randomized design was used as a statistical model with four replications, and each plot consisted of five bunches individually stored in ventilated clamshell-packaged. The dual release SO2-generating pads are efficient in controlling the gray mold in ‘Rubi’ table grapes regardless of the type of perforation of the plastic liners, with low mass loss and shattered berries, with good conservation of the freshness of the rachis. The disease was efficiently controlled in both annual crops. The slow-release SO2-generating pads, regardless of the type of perforation of the plastic liners, resulted in intermediate efficiency of gray mold control, with good physical quality of the bunches. Thus, the use of dual release SO2-generating pads is recommended to control gray mold in ventilated clamshell-packaged ‘Rubi’ table grapes.
Débora Thaís Mühlbeier; Universidade Estadual de Londrina; Luana Tainá Ribeiro; Maíra Tiaki Higuchi; Youssef Khamis; Osmar José Chaves Junior; Renata Koyama; Sergio Ruffo Roberto. SO2-generating pads reduce gray mold in clamshell-packaged ‘Rubi’ table grapes grown under a two-cropping per year system. Semina: Ciências Agrárias 2021, 42, 1069 -1086.
AMA StyleDébora Thaís Mühlbeier, Universidade Estadual de Londrina, Luana Tainá Ribeiro, Maíra Tiaki Higuchi, Youssef Khamis, Osmar José Chaves Junior, Renata Koyama, Sergio Ruffo Roberto. SO2-generating pads reduce gray mold in clamshell-packaged ‘Rubi’ table grapes grown under a two-cropping per year system. Semina: Ciências Agrárias. 2021; 42 (3):1069-1086.
Chicago/Turabian StyleDébora Thaís Mühlbeier; Universidade Estadual de Londrina; Luana Tainá Ribeiro; Maíra Tiaki Higuchi; Youssef Khamis; Osmar José Chaves Junior; Renata Koyama; Sergio Ruffo Roberto. 2021. "SO2-generating pads reduce gray mold in clamshell-packaged ‘Rubi’ table grapes grown under a two-cropping per year system." Semina: Ciências Agrárias 42, no. 3: 1069-1086.
The main objective of this study was to evaluate Bacillus velezensis strain CMRP 4490 regarding its ability to inhibit soil-borne plant pathogens and to increase plant growth. The study included evaluation of in vitro antifungal control, sequencing the bacterial genome, mining genes responsible for the synthesis of secondary metabolites, root colonization ability, and greenhouse studies for the assessment of plant growth–promoting ability. The strain was obtained from soil samples in the north of Paraná in Brazil and was classified as a B. velezensis, which is considered a promising biological control agent. In vitro assay showed that B. velezensis CMRP 4490 presented antagonistic activity against Sclerotinia sclerotiorum, Macrophomina phaseolina, Botrytis cinerea, and Rhizoctonia solani with a mycelial growth inhibition of approximately 60%, without any significant difference among them. To well understand this strain and to validate its effect on growth-promoting rhizobacteria, it was decided to explore its genetic content through genome sequencing, in vitro, and greenhouse studies. The genome of CMRP 4490 was estimated at 3,996,396 bp with a GC content of 46.4% and presents 4,042 coding DNA sequences. Biosynthetic gene clusters related to the synthesis of molecules with antifungal activity were found in the genome. Genes linked to the regulation/formation of biofilms, motility, and important properties for rhizospheric colonization were also found in the genome. Application of CMRP 4490 as a coating film on soybean increased from 55.5 to 64% on germination rates when compared to the control; no differences were observed among treatments for the maize germination. The results indicated that B. velezensis CMRP 4490 could be a potential biocontrol agent with plant growth–promoting ability.
Gustavo Manoel Teixeira; Mirela Mosela; Maria Luiza Abreu Nicoletto; Renan Augusto Ribeiro; Mariangela Hungria; Khamis Youssef; Allan Yukio Higashi; Silas Mian; André Sampaio Ferreira; Leandro Simões Azeredo Gonçalves; Ulisses De Padua Pereira; Admilton Gonçalves de Oliveira. Genomic Insights Into the Antifungal Activity and Plant Growth-Promoting Ability in Bacillus velezensis CMRP 4490. Frontiers in Microbiology 2021, 11, 1 .
AMA StyleGustavo Manoel Teixeira, Mirela Mosela, Maria Luiza Abreu Nicoletto, Renan Augusto Ribeiro, Mariangela Hungria, Khamis Youssef, Allan Yukio Higashi, Silas Mian, André Sampaio Ferreira, Leandro Simões Azeredo Gonçalves, Ulisses De Padua Pereira, Admilton Gonçalves de Oliveira. Genomic Insights Into the Antifungal Activity and Plant Growth-Promoting Ability in Bacillus velezensis CMRP 4490. Frontiers in Microbiology. 2021; 11 ():1.
Chicago/Turabian StyleGustavo Manoel Teixeira; Mirela Mosela; Maria Luiza Abreu Nicoletto; Renan Augusto Ribeiro; Mariangela Hungria; Khamis Youssef; Allan Yukio Higashi; Silas Mian; André Sampaio Ferreira; Leandro Simões Azeredo Gonçalves; Ulisses De Padua Pereira; Admilton Gonçalves de Oliveira. 2021. "Genomic Insights Into the Antifungal Activity and Plant Growth-Promoting Ability in Bacillus velezensis CMRP 4490." Frontiers in Microbiology 11, no. : 1.
Disinfection after harvest is an essential step to maintain commodities and facilities free of fungal and bacterial postharvest pathogens, responsible of storage decay and economic losses. Electrolyzed water (EW) has gained considerable interest over the last decades as a novel broad-spectrum sanitizer. EW is sustainable and cost effective since it can be produced on-site utilizing tap water and different inexpensive salts and is healthy for both the environment and human beings. Its effectiveness in controlling fungi, yeasts, and bacteria within a wide range of pH is due to multiple mode of actions. Furthermore, its strong oxidizing potential is capable to reduce the amount of pesticide residues on fruit and vegetable surfaces and to avoid pathogen resistance. Properties of EW are related to salts employed for production, being those with low chlorine content preferable. Lastly, EW has no negative effect on the organoleptic properties and features of treated commodities. The present chapter highlights recent developments in EW generation, factors affecting its effectiveness for controlling postharvest decay of fruits and vegetables, mechanism of action on microbes and hosts, and advantages and disadvantages on its use.
Antonio Ippolito; Annamaria Mincuzzi; Antony Surano; Khamis Youssef; Simona Marianna Sanzani. Electrolyzed Water as a Potential Agent for Controlling Postharvest Decay of Fruits and Vegetables. Postharvest Pathology 2020, 181 -202.
AMA StyleAntonio Ippolito, Annamaria Mincuzzi, Antony Surano, Khamis Youssef, Simona Marianna Sanzani. Electrolyzed Water as a Potential Agent for Controlling Postharvest Decay of Fruits and Vegetables. Postharvest Pathology. 2020; ():181-202.
Chicago/Turabian StyleAntonio Ippolito; Annamaria Mincuzzi; Antony Surano; Khamis Youssef; Simona Marianna Sanzani. 2020. "Electrolyzed Water as a Potential Agent for Controlling Postharvest Decay of Fruits and Vegetables." Postharvest Pathology , no. : 181-202.
The main purpose of this study was to explore the ability of a novel silica/polysaccharide polymer-based formulation, namely, chitosan/silica nanocomposites (CSNs), to directly affect Botrytis cinerea in vitro and in inoculated berries and indirectly to induce natural host resistance via enzymatic and nonenzymatic antioxidants against gray mold of table grapes. The results indicated a positive correlation in in vitro tests in terms of radial growth, spore germination and germ tube elongation, where those parameters were completely inhibited by CSN at 1%. SEM and TEM investigations showed that morphological and internal structural damage was observed in B. cinerea-hyphae/spores treated with CSN. Additionally, most of the treated spores were affected, and cellular vacuolization and cytoplasmic disorganization were observed. The results revealed that CSN reduced gray mold incidence and severity on inoculated berries directly and indirectly. The results revealed that CSN reduced gray mold incidence and severity on inoculated berries directly and indirectly. In direct activity, CSN (1%) reduced mold incidence and severity by 100% compared to the control. In indirect activity, mold incidence and severity was reduced by 51% and 64%, respectively. CSN significantly increased superoxide dismutase, ascorbate peroxidase, peroxidase, total phenol and flavonoid at 48 h posttreatment by 1.2-, 1.6-, 1.3-, 1.3- and 1.6-fold, respectively, in grape-treated tissues. It could be concluded that CSN, as a promising alternative control method against gray mold of table grapes, can directly affect the pathogen and indirectly enhance the natural host resistance of the antioxidant system.
Khamis Youssef; Sergio Ruffo Roberto. Chitosan/silica nanocomposite-based formulation alleviated gray mold through stimulation of the antioxidant system in table grapes. International Journal of Biological Macromolecules 2020, 168, 242 -250.
AMA StyleKhamis Youssef, Sergio Ruffo Roberto. Chitosan/silica nanocomposite-based formulation alleviated gray mold through stimulation of the antioxidant system in table grapes. International Journal of Biological Macromolecules. 2020; 168 ():242-250.
Chicago/Turabian StyleKhamis Youssef; Sergio Ruffo Roberto. 2020. "Chitosan/silica nanocomposite-based formulation alleviated gray mold through stimulation of the antioxidant system in table grapes." International Journal of Biological Macromolecules 168, no. : 242-250.
To obtain a clear understanding of the mode of action of potassium bicarbonate (PB), sodium silicate (SSi) and calcium chelate (CCh) solutions (1%) in inducing resistance to gray mold disease in table grapes, enzymatic and nonenzymatic investigations were carried out. In particular, changes in the activity of the enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POD), total phenolic content and total flavonoid content were studied. As indirect action, PB, SSi and CCh reduced the incidence of gray mold by 43%, 50% and 41%, respectively. The highest activity of SOD was detected at 48 h in SSi-treated tissue, PB-treated tissue and CCh-treated tissue, and it was 1.7-, 1.4- and 1.2-fold higher, respectively, compared to the control. The APX activity was significantly higher in SSi-treated tissue than in the control at 24, 48 and 72 h and showed an increase in activity 2-fold for all times. Additionally, PB, SSi and CCh increased the activity of POD by 1.4-, 1.2- and 2.7-fold at 48 h posttreatment, respectively. The results showed that CCh was the most pronounced salt to increase both total phenol and flavonoid contents by 1.3 and 2.1, respectively. Additionally, the three tested salts induced an increase in total phenols and total flavonoids at 48 h posttreatment. The obtained result is one more movement towards an overall understanding of the mechanism by which salt solutions act as antimicrobial agents against gray mold of table grapes.
Khamis Youssef; Sergio Ruffo Roberto; Angélica Nunes Tiepo; Leonel Vinicius Constantino; Juliano Tadeu Vilela De Resende; Kamal A.M. Abo-Elyousr. Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes. Journal of Fungi 2020, 6, 179 .
AMA StyleKhamis Youssef, Sergio Ruffo Roberto, Angélica Nunes Tiepo, Leonel Vinicius Constantino, Juliano Tadeu Vilela De Resende, Kamal A.M. Abo-Elyousr. Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes. Journal of Fungi. 2020; 6 (3):179.
Chicago/Turabian StyleKhamis Youssef; Sergio Ruffo Roberto; Angélica Nunes Tiepo; Leonel Vinicius Constantino; Juliano Tadeu Vilela De Resende; Kamal A.M. Abo-Elyousr. 2020. "Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes." Journal of Fungi 6, no. 3: 179.
The aim of this study is to assess the effect of extracts of Nerium oleander, Eucalyptus chamadulonsis and Citrullus colocynthis against bacterial spot disease of tomato and to investigate the induction of resistance by tomato (Solanum lycopersicum) in order to promote a sustainable management system. The antibacterial activity of aqueous and ethanol plant extracts was tested against Xanthomonas axonopodis pv. vesicatoria, isolate PHYXV3, in vitro and in vivo. The highest antibacterial activity in vitro was obtained with C. colocynthis, N. oleander and E. chamadulonsis, respectively. In vivo, ethanol extracts of N. oleander and E. chamadulonsis were more effective than aqueous extracts in reducing pathogen populations on tomato leaves. Under greenhouse conditions, application of the plant extracts at 15% (v/v) to tomato plants significantly reduced disease severity and increased the shoot weight of ‘Super Marmande’ tomato. In most cases, plant extracts significantly increased total phenol and salicylic acid content of tomato plants compared to either healthy or infected ones. In addition, C. colocynthis and E. chamadulonsis extracts significantly increased peroxidase activity while only E. chamadulonsis increased polyphenol oxidase after infection with the causal agent. The results indicated that the plant extracts showed promising antibacterial activity and could be considered an effective tool in integrated management programs for a sustainable system of tomato bacterial spot control.
Kamal A. M. Abo-Elyousr; Najeeb Almasoudi; Ahmed W. M. Abdelmagid; Sergio R. Roberto; Khamis Youssef. Plant Extract Treatments Induce Resistance to Bacterial Spot by Tomato Plants for a Sustainable System. Horticulturae 2020, 6, 36 .
AMA StyleKamal A. M. Abo-Elyousr, Najeeb Almasoudi, Ahmed W. M. Abdelmagid, Sergio R. Roberto, Khamis Youssef. Plant Extract Treatments Induce Resistance to Bacterial Spot by Tomato Plants for a Sustainable System. Horticulturae. 2020; 6 (2):36.
Chicago/Turabian StyleKamal A. M. Abo-Elyousr; Najeeb Almasoudi; Ahmed W. M. Abdelmagid; Sergio R. Roberto; Khamis Youssef. 2020. "Plant Extract Treatments Induce Resistance to Bacterial Spot by Tomato Plants for a Sustainable System." Horticulturae 6, no. 2: 36.
The aim of this research was to determine the incidence and possible causal pathogen(s) of premature apple fruit drop (PAFD), and also to assess some fungicides for controlling the disease organisms, in order to promote a sustainable system in orchards. The prevalence and natural incidence of apple fruit drop in cv. Anna was assessed during the 2017–2018 growing seasons in Nubaria and Cairo–Alexandria regions, Egypt. Phytopathogenic fungi were isolated from dropped fruit, and four fungicides, pyraclostrobin + boscalid, difenoconazole, carbendazim, and thiophanate methyl, were tested against the diseases in vitro and under naturally occurring infections in the field. Several phytopathogenic fungi, including Alternaria alternata, Cladosporium cladosporioides, Fusarium semitectum, and Penicillium spp., were associated with apple fruit drop. A. alternata was the most frequently isolated fungus occurring during the investigation. Pathogenicity tests confirmed that the maximum percentage of apple fruit drop was noted when petioles and fruits were inoculated with mixed fungal pathogens using branch sections with fruit. In vitro tests showed that the fungicides had a variable effect against the fungal isolates depending on the concentration used. All fungicides completely inhibited the growth of A. alternata, C. cladosporioides, and F. semitectum at 400 mg·L−1. Under naturally occurring infections, thiophanate methyl applied at fruit set had the greatest effect (81.68%) against PAFD, followed by difenoconazole (73.76%), pyraclostrobin + boscalid (70.29%), and carbendazim (66.34%). The results indicated that PAFD may in part be a result of diseases caused by certain phytopathogenic fungi, which could be controlled using a number of fungicides applied at the beginning of fruit set.
Khamis Youssef; Sergio Ruffo Roberto. Premature Apple Fruit Drop: Associated Fungal Species and Attempted Management Solutions. Horticulturae 2020, 6, 31 .
AMA StyleKhamis Youssef, Sergio Ruffo Roberto. Premature Apple Fruit Drop: Associated Fungal Species and Attempted Management Solutions. Horticulturae. 2020; 6 (2):31.
Chicago/Turabian StyleKhamis Youssef; Sergio Ruffo Roberto. 2020. "Premature Apple Fruit Drop: Associated Fungal Species and Attempted Management Solutions." Horticulturae 6, no. 2: 31.
Present sterilization practices involve the use of chlorine/hypochlorite, chlorine dioxide, peracetic acid, hydrogen peroxide, quaternary ammonium salts, ozone, and UV radiation. A new generation of techniques for ensuring food security and quality, particularly microbial control medication, is seemingly within easy reach that can empower the food industry, address current insufficiencies and losses, and reach new levels of well-being, sustainability and economic growth (Eleftheriadou et al. 2017). Another innovation demonstrates that microorganisms on fresh produce and production surfaces can be inactivated by electrolyzed water and water nanostructures produced by electrospraying water vapor (Glaser 2015). Acidic electrolyzed water (AEW) is being studied as a promising disinfecting agent in the food, medical and agricultural industries (Wang et al. 2014a). Results suggest that AEW treatment could altogether decrease populations of Escherichia coli O157 H7, Salmonella 118typhimurium, and Listeria monocytogenes pathogens from the surfaces of lettuce and spinach leaves with increasing time of exposure, while alkaline electrolyzed water did not decrease pathogen levels even after a 5 min treatment on lettuce and spinach (Park et al. 2008). Acidic electrolyzed water has been recognized to be an effective disinfectant for inactivating food-borne pathogens including Escherichia coli, Vibrio parahaemolyticus, and Listeria monocytogenes (Wang et al. 2014b).
Kamel A. Abd-Elsalam; Khamis Youssef; Farah K. Ahmed; Hassan Almoammar. Microbially Inspired Nanostructures for Management of Food-Borne Pathogens. Microbial Nanotechnology 2020, 117 -134.
AMA StyleKamel A. Abd-Elsalam, Khamis Youssef, Farah K. Ahmed, Hassan Almoammar. Microbially Inspired Nanostructures for Management of Food-Borne Pathogens. Microbial Nanotechnology. 2020; ():117-134.
Chicago/Turabian StyleKamel A. Abd-Elsalam; Khamis Youssef; Farah K. Ahmed; Hassan Almoammar. 2020. "Microbially Inspired Nanostructures for Management of Food-Borne Pathogens." Microbial Nanotechnology , no. : 117-134.
The potential integration of the benefits of inorganic materials together with those of natural polymers can empower a wide scope of utilization for these nanocomposites. These materials have been comprehensively investigated because of the sole integration of properties that can take place, especially when the inorganic areas have a nanoscale measurement. Hybrid inorganic polymer-based molecules are generally utilized as safe and ecofriendly tools to improve crop production in various agricultural produce. This chapter summarizes the preparation, characterization, and plant-protection applications of hybrid polymer nanocomposites. Several plans have been formed about the synthesis of polymer-based inorganic nanocomposites, which will be discussed in this chapter. The improvement and commercialization of these items require profoundly specialized item advancement, advertising advancement, and market instruction. Hybrid inorganic-polymer nanocomposites against plant pathogenic fungi and bacteria will be reviewed in detail.
Ayat F. Hashim; Khamis Youssef; Sergio Ruffo Roberto; Kamel A. Abd-Elsalam. Hybrid inorganic-polymer nanocomposites: Synthesis, characterization, and plant-protection applications. Multifunctional Hybrid Nanomaterials for Sustainable Agri-Food and Ecosystems 2020, 33 -49.
AMA StyleAyat F. Hashim, Khamis Youssef, Sergio Ruffo Roberto, Kamel A. Abd-Elsalam. Hybrid inorganic-polymer nanocomposites: Synthesis, characterization, and plant-protection applications. Multifunctional Hybrid Nanomaterials for Sustainable Agri-Food and Ecosystems. 2020; ():33-49.
Chicago/Turabian StyleAyat F. Hashim; Khamis Youssef; Sergio Ruffo Roberto; Kamel A. Abd-Elsalam. 2020. "Hybrid inorganic-polymer nanocomposites: Synthesis, characterization, and plant-protection applications." Multifunctional Hybrid Nanomaterials for Sustainable Agri-Food and Ecosystems , no. : 33-49.
The selection of superior strawberry genotypes is a complex process due to the high variability after hybridization that is caused by the octoploid nature and the heterozygosis, making the selection of multiple traits difficult. This study aimed to select strawberry hybrids with the potential for fresh consumption and/or processing by applying multivariate analysis to obtain traits of interest simultaneously. Hybrids were obtained from the crossing among seven commercial cultivars, defining a selection of 10% of them. The experimental design consisted of an augmented block design, with two commercial cultivars, Camarosa and Camino Real, as the controls. Different variables, including the number and average mass of commercial fruits, total fruit mass, pH, soluble solids (SS), titratable acidity (TA), SS/TA ratio, reducing sugars, pectin, ascorbic acid, phenolic compounds, and anthocyanin’s, were assessed. The selection of hybrids was based on the Mulamba and Mock rank-summation index, principal component analysis, and Ward’s hierarchical cluster analysis. The selection index was based on different weights being adopted for fresh market and processing. The assessed traits had high variability between hybrids. The highest selection gains were obtained for production traits, but the different weight assignment resulted in different classifications of hybrids for both fresh consumption and processing. Most of the hybrids selected by the index remained in the same group in the principal component and hierarchical cluster analyses, which indicates that multivariate analysis is a valuable tool for assisting in the selection of superior hybrids in the strawberry crop.
Eneide Barth; Juliano Tadeu Vilela De Resende; Aline Fabiana Paladini Moreira; Keny Henrique Mariguele; André Ricardo Zeist; Mayara Barbosa Silva; Gianne Caroline Guidoni Stulzer; João Gabriel Macedo Mafra; Leandro Simões Azeredo Gonçalves; Sergio Ruffo Roberto; Khamis Youssef. Selection of Experimental Hybrids of Strawberry Using Multivariate Analysis. Agronomy 2020, 10, 598 .
AMA StyleEneide Barth, Juliano Tadeu Vilela De Resende, Aline Fabiana Paladini Moreira, Keny Henrique Mariguele, André Ricardo Zeist, Mayara Barbosa Silva, Gianne Caroline Guidoni Stulzer, João Gabriel Macedo Mafra, Leandro Simões Azeredo Gonçalves, Sergio Ruffo Roberto, Khamis Youssef. Selection of Experimental Hybrids of Strawberry Using Multivariate Analysis. Agronomy. 2020; 10 (4):598.
Chicago/Turabian StyleEneide Barth; Juliano Tadeu Vilela De Resende; Aline Fabiana Paladini Moreira; Keny Henrique Mariguele; André Ricardo Zeist; Mayara Barbosa Silva; Gianne Caroline Guidoni Stulzer; João Gabriel Macedo Mafra; Leandro Simões Azeredo Gonçalves; Sergio Ruffo Roberto; Khamis Youssef. 2020. "Selection of Experimental Hybrids of Strawberry Using Multivariate Analysis." Agronomy 10, no. 4: 598.
The purpose of this research is to test the efficacy of different types of SO2-generating pads on the incidence of gray mold, and on the physicochemical properties of quality of ‘BRS Nubia’ seeded table grapes grown under protected cultivation. Four types of SO2-generating pads, 5 or 8 g of sodium metabisulfite dual release pads, and 4 or 7 g of sodium metabisulfite slow release pads, were used. Grapes bunches were harvested from a vineyard covered with plastic mash and stored in a cold room at 1 ± 1 °C for 45 days followed by 6 days of shelf life at 22 ± 1 °C at a high relative humidity (>95%). The results showed that SO2-generating pads with a dual release of 5 or 8 g completely inhibited the development of gray mold at all evaluation times. Also, a high reduction of the disease incidence was achieved by using a slow release of 4 g. The study confirmed that SO2-generating pads did not alter the physicochemical properties of ‘BRS Nubia’ seeded table grapes including mass loss, berry firmness, color index, total anthocyanin concentration, total soluble solids (TSS), titratable acidity (TA), and the TSS/TA ratio. Slow release pads at 4 and 7 g reduced the percentage of shattered berries by 56 and 48% as compared to control only after 6 days of shelf life. Also, all types of SO2-generating pads reduced the stem browning score at the end of cold storage. The 5 or 8 g dual release pads and 4 g slow release pads can be considered for effective controlling of gray mold for ‘BRS Nubia’ table grapes grown under protected cultivation while maintaining grape quality.
Khamis Youssef; Osmar Jose Chaves Junior; Débora Thaís Mühlbeier; Sergio Ruffo Roberto. Sulphur Dioxide Pads Can Reduce Gray Mold While Maintaining the Quality of Clamshell-Packaged ‘BRS Nubia’ Seeded Table Grapes Grown under Protected Cultivation. Horticulturae 2020, 6, 20 .
AMA StyleKhamis Youssef, Osmar Jose Chaves Junior, Débora Thaís Mühlbeier, Sergio Ruffo Roberto. Sulphur Dioxide Pads Can Reduce Gray Mold While Maintaining the Quality of Clamshell-Packaged ‘BRS Nubia’ Seeded Table Grapes Grown under Protected Cultivation. Horticulturae. 2020; 6 (2):20.
Chicago/Turabian StyleKhamis Youssef; Osmar Jose Chaves Junior; Débora Thaís Mühlbeier; Sergio Ruffo Roberto. 2020. "Sulphur Dioxide Pads Can Reduce Gray Mold While Maintaining the Quality of Clamshell-Packaged ‘BRS Nubia’ Seeded Table Grapes Grown under Protected Cultivation." Horticulturae 6, no. 2: 20.
Khamis Youssef; Zaki M. M. Mustafa; Mohamed A. M. Kamel; Gehan A. Mounir. Cigar end rot of banana caused by Musicillium theobromae and its control in Egypt. Archives Of Phytopathology And Plant Protection 2020, 53, 162 -177.
AMA StyleKhamis Youssef, Zaki M. M. Mustafa, Mohamed A. M. Kamel, Gehan A. Mounir. Cigar end rot of banana caused by Musicillium theobromae and its control in Egypt. Archives Of Phytopathology And Plant Protection. 2020; 53 (3-4):162-177.
Chicago/Turabian StyleKhamis Youssef; Zaki M. M. Mustafa; Mohamed A. M. Kamel; Gehan A. Mounir. 2020. "Cigar end rot of banana caused by Musicillium theobromae and its control in Egypt." Archives Of Phytopathology And Plant Protection 53, no. 3-4: 162-177.
The two-spotted spider mite (Tetranychus urticae) is the main pest encountered in strawberry cultivation in protected environments, causing damage and increasing the cost of production associated with chemical control. Breeding programs are essential for obtaining new cultivars that are more tolerant to two-spotted spider mites. The objective was to evaluate whether the leaf trichomes mediatethe resistance of intraspecific strawberry hybrids to two-spotted spider mites. Preselected hybrids were subjected to infestation by two-spotted spider mites and evaluated via the no-choice assay and the travel test. Resistance was correlated with the density of trichomes, both glandular and non-glandular, present on the surface of the leaflets of strawberry genotypes. The results indicated that there is an influence of hybrids and cultivars on the behavior of the pest, allowing the verification of the existence of resistant genotypes (RVDA#16, RVCS#44 and Camino Real). These treatments resulted in low numbers of eggs deposited and hatched compared with the other treatments and shorter distances traveled by two-spotted spider mites on the surface of leaflets. It was also verified that the glandular trichomes presented a significant negative correlation with the average distance traveled by two-spotted spider mites on the surface of the leaf discs, indicating that these structures alter the behavior of these arthropods. Genotype resistance was associated with the presence of glandular trichomes. In addition to the superiority of the agronomic and postharvest attributes of the preselected hybrids (RVDA#16 and RVCS#44) identified in the previous selection efforts, they proved to be promising materials for achieving resistance to the spider mite, revealing important genotypes for use as cultivars and particularly as parents in breeding programs.
Juliano Tadeu Vilela De Resende; Renato Barros De Lima Filho; Luana Karolline Ribeiro; Jéssica Vanessa Wosniak Corrêa; Cleber Daniel De Goes Maciel; Khamis Youssef. Strawberry genotypes with resistance to Tetranychus urticae mediated by leaf trichomes. Ciência e Agrotecnologia 2020, 44, 1 .
AMA StyleJuliano Tadeu Vilela De Resende, Renato Barros De Lima Filho, Luana Karolline Ribeiro, Jéssica Vanessa Wosniak Corrêa, Cleber Daniel De Goes Maciel, Khamis Youssef. Strawberry genotypes with resistance to Tetranychus urticae mediated by leaf trichomes. Ciência e Agrotecnologia. 2020; 44 ():1.
Chicago/Turabian StyleJuliano Tadeu Vilela De Resende; Renato Barros De Lima Filho; Luana Karolline Ribeiro; Jéssica Vanessa Wosniak Corrêa; Cleber Daniel De Goes Maciel; Khamis Youssef. 2020. "Strawberry genotypes with resistance to Tetranychus urticae mediated by leaf trichomes." Ciência e Agrotecnologia 44, no. : 1.
Khamis Youssef; Ayat F. Hashim; Sergio Ruffo Roberto; Said Fatouh Hamed; Kamel A. Abd-Elsalam. Graphene-based nanocomposites: Synthesis, characterizations, and their agri-food applications. Carbon Nanomaterials for Agri-Food and Environmental Applications 2020, 33 -57.
AMA StyleKhamis Youssef, Ayat F. Hashim, Sergio Ruffo Roberto, Said Fatouh Hamed, Kamel A. Abd-Elsalam. Graphene-based nanocomposites: Synthesis, characterizations, and their agri-food applications. Carbon Nanomaterials for Agri-Food and Environmental Applications. 2020; ():33-57.
Chicago/Turabian StyleKhamis Youssef; Ayat F. Hashim; Sergio Ruffo Roberto; Said Fatouh Hamed; Kamel A. Abd-Elsalam. 2020. "Graphene-based nanocomposites: Synthesis, characterizations, and their agri-food applications." Carbon Nanomaterials for Agri-Food and Environmental Applications , no. : 33-57.
Post-harvest diseases of fruit and vegetables have to be controlled because of the high added value of commodities and the great economic loss related to spoilage. Synthetic fungicides are the first choice worldwide to control post-harvest diseases of fruit and vegetables. However, several problems and constraints related to their use have forced scientists to develop alternatives control means to prevent post-harvest diseases. Physical and biological means, resistance inducers, and GRAS (generally recognized as safe) compounds are the most important alternatives used during the last 20 years. Recently, nanomaterial treatments have demonstrated promising results and they are being investigated to reduce the utilization of synthetic fungicides to control post-harvest rot in fruit and vegetables. The collective information in this review article covers a wide range of nanomaterials used to control post-harvest decays related to each selected fruit crop including grape, citrus, banana, apple, mango, peach, and nectarine. Other examples also used are apricot, guava, avocado, papaya, dragon, pear, longan, loquat, jujubes, and pomegranate fruits.
Sergio Ruffo Roberto; Khamis Youssef; Ayat Farghily Hashim; Antonio Ippolito. Nanomaterials as Alternative Control Means Against Postharvest Diseases in Fruit Crops. Nanomaterials 2019, 9, 1752 .
AMA StyleSergio Ruffo Roberto, Khamis Youssef, Ayat Farghily Hashim, Antonio Ippolito. Nanomaterials as Alternative Control Means Against Postharvest Diseases in Fruit Crops. Nanomaterials. 2019; 9 (12):1752.
Chicago/Turabian StyleSergio Ruffo Roberto; Khamis Youssef; Ayat Farghily Hashim; Antonio Ippolito. 2019. "Nanomaterials as Alternative Control Means Against Postharvest Diseases in Fruit Crops." Nanomaterials 9, no. 12: 1752.
The use of vented clamshells has become popular in the packaging of grapes for local and international markets. The aim of this study is to evaluate the postharvest preservation of ‘Benitaka’ table grapes individually packaged in vented clamshells using different types of SO2-generating pads and perforated plastic liners during cold storage. A completely randomized design with four replications in a two-factor arrangement with an additional treatment [(4 × 3) + 1] was used. The trials were carried out under two situations: Artificial or natural infections with Botrytis cinerea, which is the causal agent of gray mold on table grapes. The incidence of gray mold, shattered berries, and stem browning were evaluated at 30 and 45 days of cold storage at 1 ± 1 °C and 3 days of shelf-life at 22 ± 1 °C after the period of cold storage. Mass loss and berry firmness were also examined at the end of the cold storage period. The use of dual-release SO2-generating pads containing 5 or 8 g of a.i. and slow-release pads with 7 g of a.i. was effective in controlling the incidence of gray mold in grapes packaged in vented clamshells and kept under cold storage for up to 45 days. Under these storage conditions, perforated plastic liners with 0.3% ventilation area or micro-perforated liners with 1.0% ventilation area reduced the percentage of mass loss and shattered berries.
Osmar Jose Chaves Junior; Khamis Youssef; Renata Koyama; Saeed Ahmed; Allan Ricardo Dominguez; Débora Thaís Mühlbeier; Sergio Ruffo Roberto. Control of Gray Mold on Clamshell-Packaged ‘Benitaka’ Table Grapes Using Sulphur Dioxide Pads and Perforated Liners. Pathogens 2019, 8, 271 .
AMA StyleOsmar Jose Chaves Junior, Khamis Youssef, Renata Koyama, Saeed Ahmed, Allan Ricardo Dominguez, Débora Thaís Mühlbeier, Sergio Ruffo Roberto. Control of Gray Mold on Clamshell-Packaged ‘Benitaka’ Table Grapes Using Sulphur Dioxide Pads and Perforated Liners. Pathogens. 2019; 8 (4):271.
Chicago/Turabian StyleOsmar Jose Chaves Junior; Khamis Youssef; Renata Koyama; Saeed Ahmed; Allan Ricardo Dominguez; Débora Thaís Mühlbeier; Sergio Ruffo Roberto. 2019. "Control of Gray Mold on Clamshell-Packaged ‘Benitaka’ Table Grapes Using Sulphur Dioxide Pads and Perforated Liners." Pathogens 8, no. 4: 271.
Potassium bicarbonate (PB), calcium chelate (CCh), and sodium silicate (SSi) have been extensively used as antifungal generally recognized as safe (GRAS) compounds against plant pathogenic fungi. In this research, in in vitro tests, the radial growth, conidial germination, and germ tube elongation of Botrytis cinerea was completely inhibited at 0.3% of PB, SSi, and CCh. In in vivo tests, application of PB, SSi, and CCh completely inhibited the occurrence of gray mold incidence of inoculated ‘Italia’ grape berries at concentrations of 1.0, 0.8, and 0.8%, respectively. In order to investigate the detailed mechanisms by which salts exhibited antifungal activity, we analyzed their influence on morphological changes by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) and also on reactive species of oxygen (ROS), mitochondrial membrane potential (MMP), and adenosine triphosphate (ATP) content. Defects such as malformation and excessive septation were detected on salt-treated hyphae morphology observed by SEM. The internal structure of conidia treated or not with salt solutions was examined by TEM. In treated conidia, most of the conidia were affected and cellular vacuolization and cytoplasmic disorganization was observed. For ROS accumulation, a higher increase was observed in fluorescent conidia in presence of PB, SSi, and CCh by 75, 68, and 70% as compared to control, respectively. MMP was significantly decreased after salt application indicating a loss of mitochondria function. Also, luminescence showed that B. cinerea-conidia treated with salts contained less ATP than the untreated conidia. The results obtained herein are a step towards a comprehensive understanding of the mode of action by which salts act as antifungal agents against B. cinerea.
Khamis Youssef; Sergio Ruffo Roberto; Admilton G. De Oliveira. Ultra-Structural Alterations in Botrytis cinerea—The Causal Agent of Gray Mold—Treated with Salt Solutions. Biomolecules 2019, 9, 582 .
AMA StyleKhamis Youssef, Sergio Ruffo Roberto, Admilton G. De Oliveira. Ultra-Structural Alterations in Botrytis cinerea—The Causal Agent of Gray Mold—Treated with Salt Solutions. Biomolecules. 2019; 9 (10):582.
Chicago/Turabian StyleKhamis Youssef; Sergio Ruffo Roberto; Admilton G. De Oliveira. 2019. "Ultra-Structural Alterations in Botrytis cinerea—The Causal Agent of Gray Mold—Treated with Salt Solutions." Biomolecules 9, no. 10: 582.