Biogenic and biogenic-thermogenic coalbed methane (CBM) are important energy reserves for unconventional natural gas. Thus, to investigate biogenic gas formation mechanisms, a series of fresh coal samples from several representative areas of China were analyzed to detect hydrogen-producing bacteria and methanogens in an in situ coal seam. Complete microbial DNA sequences were extracted from enrichment cultures grown on coal using the Miseq high-throughput sequencing technique to study the diversity of microbial communities. The species present and differences between the dominant hydrogen-producing bacteria and methanogens in the coal seam are then considered based on environmental factors. Sequences in the Archaea domain were classified into four phyla and included members from Euryarchaeota, Thaumarchaeota, Woesearchaeota, and Pacearchaeota. The Bacteria domain included members of the phyla: Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Acidobacteria, Verrucomicrobia, Planctomycetes, Chloroflexi, and Nitrospirae. The hydrogen-producing bacteria was dominated by the genera: Clostridium, Enterobacter, Klebsiella, Citrobacter, and Bacillus; the methanogens included the genera: Methanorix, Methanosarcina, Methanoculleus, Methanobrevibacter, Methanobacterium, Methanofollis, and Methanomassiliicoccus. Traces of hydrogen-producing bacteria and methanogens were detected in both biogenic and non-biogenic CBM areas. The diversity and abundance of bacteria in the biogenic CBM areas are relatively higher than in the areas without biogenic CBM. The community structure and distribution characteristics depend on coal rank, trace metal elements, temperature, depth and groundwater dynamic conditions. Biogenic gas was mainly composed of hydrogen and methane, the difference and diversity were caused by microbe-specific fermentation of substrates; as well as by the environmental conditions. This discovery is a significant contribution to extreme microbiology, and thus lays the foundation for research on biogenic CBM.

The identification of the dust characteristics in coal mine working faces is essential for preventing coal dust explosion and occupational diseases. In this paper, dust samples from the coal mines in southern Shanxi province and Henan province, central North China, were selected as the research objects. The results show that the dust contains primarily organic matter, as well as considerable amounts of minerals. The chemical composition of dust at the working faces is the most complex. According to the proportion of PM₁₀, the dust composition can be divided into three types: symmetrical, fine-dominated, and coarse-dominated. The wettability of dust increases with the increase of the oxygen–carbon ratio on its surface, increase of ash content, decrease of fixed carbon content, and decrease of particle size. In addition, the great variety of harmful elements in dust, some with a high content, can harm the human body. An explosion index is proposed to evaluate the likeliness tendency of coal dust explosion based on several key affecting factors. The surfactant (0.05% AN solution) adopted in this paper can significantly increase the wettability of coal dust and inhibit the generation of dust greatly, showing good ability in preventing coal dust explosion and occupational diseases.