Interest in improving plant nitrogen use efficiency (NUE) in conjunction with reduced usage of nitrogen (N) fertilizers in forestry management is growing. Although biochar amendment is widely applied to increase soil nutrient availability and NUE, the mechanism underlying their positive effects remains little understood. We treated the economically important eucalypt species with biochar (BC), N-enriched fertilizer with ¹⁵N isotope labeling (NF), and biochar plus ¹⁵N-labeled fertilizer (NFB). Moreover, we determined plant N absorption and soil N availability, soil bacterial community composition and its putative keystone taxa, and plant NUE and competition index under different treatments. Our results indicated that NF and NFB significantly increased plant atom % ¹⁵N in both eucalypt stem, root, and foliar, as well as the competition index of eucalypt to forbs for acquiring N. NF and BC increased the network complexity of keystone taxa by shifting putative keystone taxa, including phylum Proteobacteria, Chloroflexi, Actinobacteria, Acidobacteria, and Firmicutes. Piecewise structural equation modeling indicated that variations in plant performance were best directly and positively predicted by soil Proteobacteria. This study highlights the importance of interactive effects between biochar and N fertilizer on plant performance mediated by soil microbial community. The change in soil putative keystone taxa has the potential to be a suitable predictor for plant performance in terms of biochar. Our findings may provide important implications for improving fertilization and afforestation management.

Biochar and plant growth-promoting rhizobacteria (PGPR) are widely used as an amendment for soil physicochemical properties and soil bacterial community diversity. In Guangxi, China, we carried out a study to determine how PGPR and biochar influence the soil’s environmental stability in an Eucalypt plantation. We applied biochar and PGPR in a contrasting application manner to an acidic red loam soil. Thus, three treatments were set up as 5 × 10¹⁰ CFU·mL⁻¹ PGPR-only (MB0), 20 t·hm⁻² biochar-only (B20), and co-application of 20 t·hm⁻² biochar and 5 × 10¹⁰ CFU·mL⁻¹ PGPR (MB20), as well as no biochar and no PGPR (M0B0). Our results indicated that MB20 significantly decreased the soil total nitrogen (TN) and increased the soil total phosphorus (Soil TP), soil ammonium nitrogen (NH₄⁺), and soil water content (SWC) when compared with the control. The MB20 also significantly increased the Simpson, ACE, and Chao indices of the soil bacterial community’s diversity relative to the control. We also observed a significant effect of the Soil TN on both the bacterial community and the functional diversity in soil. These findings may indicate that assessing the soil N status is expected to be an essential indicator of the soil microenvironment’s response to biochar and PGPR applications.

Plant growth-promoting rhizobacteria (PGPR) and filtered sludge are widely used to improve soil fertility and plant yields. In this study, we evaluated the impact of sludge and/or PGPR application on the nutrient contents and enzyme activities of the soil as well as on plant growth. We planted bare-root eucalyptus seedlings in (1) soil amended with filtered sludge from Nanning sugar factory (FS), (2) soil amended with filtered sludge + PGPR (BF), and (3) non-amended soil (control). Soil fertility and eucalyptus growth were determined after 3, 6, 9, and 12 months. Results demonstrated that FS treatment significantly increased eucalyptus growth compared to the control, particularly after six months. Bacterial fertilizer (BF) also increased soil urease and sucrase activities, although differences diminished over the study period. Our findings suggest that the integration of bacterial fertilizers and filtered sludge can serve as an effective and environmentally friendly strategy to improve soil health and promote sustainable eucalyptus cultivation. This research contributes to the growing body of evidence supporting the use of bio-fertilizers in forestry practices, highlighting their potential to reduce or replace the use of chemical fertilizers while increasing plant productivity.